Search tips
Search criteria 


Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Neuron. Author manuscript; available in PMC 2012 February 20.
Published in final edited form as:
PMCID: PMC3282191

Automated analysis of cellular signals from large-scale calcium imaging data


Recent advances in fluorescence imaging permit studies of Ca2+ dynamics in large numbers of cells, in anesthetized and awake behaving animals. However, unlike for electrophysiological signals, standardized algorithms for assigning optically recorded signals to individual cells have not yet emerged. Here we describe an automated sorting procedure that combines independent component analysis and image segmentation for extracting cells’ locations and their dynamics with minimal human supervision. In validation studies using simulated data, automated sorting significantly improved estimation of cellular signals compared to conventional analysis based on image regions of interest. We used automated procedures to analyze data recorded by two-photon Ca2+ imaging in the cerebellar vermis of awake behaving mice. Our analysis yielded simultaneous Ca2+ activity traces for up to >100 Purkinje cells and Bergmann glia from single recordings. Using this approach, we found microzones of Purkinje cells that were stable across behavioral states and in which synchronous Ca2+-spiking rose significantly during locomotion.


Techniques for loading Ca2+-indicators into many cells have enabled recent imaging studies of the dynamics of hundreds of neurons and astrocytes (Gobel et al., 2007; Greenberg et al., 2008; Mrsic-Flogel et al., 2007; Nimmerjahn et al., 2009; Ohki et al., 2005; Orger et al., 2008; Stosiek et al., 2003). However, computational techniques for extracting cellular signals from Ca2+ imaging data lag behind and are mainly region of interest (ROI) analyses. These are typically manual (Dombeck et al., 2007; Gobel et al., 2007; Kerr et al., 2005; Niell and Smith, 2005), or semi-automated (Ozden et al., 2008) means of identifying cells and cannot be easily scaled to handle the largest data sets without undue human labor. Moreover, ROI analyses have largely been based on heuristic definitions of the morphology of specific cell types (Gobel et al., 2007; Ohki et al., 2005; Ozden et al., 2008), rather than general principles for decomposing a data set into constituent signal sources. Thus, current analyses are prone to cross talk in the signals extracted from adjacent cells and surrounding neuropil. The present mismatch between the capabilities for Ca2+ imaging and those for analyzing the data restricts the capacity to attain biological insights.

This situation partly resembles that of the early 1990’s, when multi-electrode techniques were blossoming but standardized spike sorting algorithms had yet to arise. Today, automated spike sorting is widely used to assign spikes to individual cells (Fee et al., 1996; Lewicki, 1998) and has enabled key advances in understanding neural coding (Batista et al., 2007; Csicsvari et al., 1998; Meister, 1996). An automated procedure for extracting cellular Ca2+ signals would be a similar enabler of scientific progress. However, the challenges in devising such a procedure are distinct from those in spike sorting.

Spike sorting routines tend to rely on two basic ideas. First, the temporal waveforms for spikes from different cells are often sufficiently dissimilar to provide a basis for spike classification. Second, the activity of individual cells is often recorded on multiple electrodes, aiding assignment of spikes based on their relative amplitudes on different recording channels. Neither approach works well for imaging data. First, Ca2+ activity waveforms are strongly dictated by intracellular Ca2+ buffering and the dye’s binding kinetics (Helmchen et al., 1996), which do not provide strong signatures of individual cells’ identities. Second, single image pixels can contain a complex mixture of signals from neuropil, neurons, astrocytes, and noise. It is nontrivial to disentangle these signals without suffering cross talk and to find the shapes and locations of each cell. A guiding principle is needed to help extract cells’ locations and activities.

We formulated such a principle by considering that intracellular [Ca2+] can transiently rise ~100-fold above background levels during cellular events such as action potentials. Brief periods of elevated [Ca2+] are typically sparsely interspersed among many more background-dominated time frames. Sparseness also holds in the spatial domain if each cell occupies only a small subset of pixels. Thus, Ca2+ signals’ sparseness should be a general attribute that is quantifiable by simple measures, such as the skewness of amplitude distributions.

This reasoning led us to an algorithm that estimates cells’ locations and activities by parsing data into a combination of statistically independent signals, each with a high sparseness. The algorithm requires no preconceptions of cells’ appearances and little user supervision, and it relies on an independent component analysis (ICA) (Bell and Sejnowski, 1995; Brown et al., 2001; Reidl et al., 2007) (Figure 1). ICA has been used previously for analyses of electroencephalography (EEG) (Makeig et al., 1997), magnetoencephalography (MEG) (Guimaraes et al., 2007) and functional magnetic resonance imaging (fMRI) (Beckmann and Smith, 2004; McKeown et al., 1998) data, but a challenge has concerned the physiological interpretation of the identified sources, which can be mixtures of signals from different recording channels or brain areas. We reasoned that for ICA analyses of Ca2+ imaging data, such interpretative issues should be much reduced, since cells’ properties can be corroborated by other experimental means, including in the same animals examined by imaging. In studies of human brain activity, corroborative data was much harder to obtain in living subjects.

Figure 1
Analytical Stages of Automated Cell Sorting

We validated our method using simulated movies mimicking Ca2+ imaging data acquired in cerebellar cortex. Our sorting procedure provided superior signal estimates and lower susceptibility to cross talk than reconstructions done by ROI analysis. We also tested our analysis on data recorded by two-photon microscopy in the cerebellar cortex of awake behaving mice, from which we extracted Ca2+ signals of up to >100 total Purkinje cells and Bergmann glia.

To illustrate our method’s utility, we applied it to study the spatiotemporal organization of Purkinje cells’ Ca2+-spiking activity in behaving mice. We found that synchronously active cells cluster into neighborhoods ~7–18 cells across in the medio-lateral dimension. We identify these as cerebellar microzones, small patches of Purkinje cells receiving similar climbing fiber input (Andersson and Oscarsson, 1978). Our data revealed that microzones of awake animals have sharply delineated medio-lateral boundaries, to a precision of about a single cell.

We addressed the longstanding question of whether microzones have stable anatomical boundaries (Andersson and Oscarsson, 1978), or are dynamic entities whose cellular constituents vary across behavioral states (Lang et al., 1999; Welsh et al., 1995). We found that during mouse locomotion microzones’ spatial organization was unchanged from that in awake but resting animals, consistent with the idea microzones are stationary anatomical units. These findings reveal basic features of cerebellar dynamics and highlight the impact of automated procedures for analyzing imaging data.


Principles for Extracting Cellular Signals

The purpose of cell sorting is to identify spatial filters and corresponding time traces that represent the locations and dynamics of individual cells (Figure 1A). Our procedure does this in four stages (Figure 1B and Supplemental Software Toolbox).

Physiological signals are buried in high-dimensional data that might contain, e.g. Nx ~105 pixels and Nt ~104 time frames. A first stage of analysis should reduce this dimensionality, since specifying cells’ spatial filters and activities would otherwise require a large set of parameters. In principle, these parameters could be found, but tuning many parameters can hamper signal extraction (Hastie et al., 2001). For dimensional reduction we used an established method, principal component analysis (PCA) (Table S1), to find and discard dimensions that mainly encoded noise (Mitra and Pesaran, 1999). Other means of dimensional reduction might also be suitable (Discussion).

PCA identifies a linear transformation of the data yielding basis vectors, the principal components, which are rank ordered by the variance of signals along each basis vector. Truncation of the highest ranked components with the smallest variances often does not interfere with an approximate reconstruction of the data. On the contrary, if ongoing background noise has smaller variance than transient but strong signals, then removing higher order components from the data can eliminate substantial noise. In our studies of cerebellum, after identifying the noise floor and truncating principal components representing noise (Figure S1) (Mitra and Pesaran, 1999), we achieved a reduced dimensionality, K, much less than Nx or Nt. However, PCA alone cannot isolate Ca2+ signals from individual cells.

PCA by itself is ill suited for cell sorting since it relies on differences in variance to identify data components; in practice, time variations in different cells’ fluorescence signals tend to be of similar amplitude. Thus, each principal component generally has a mix of signals from multiple cells. Instead, one seeks a set of signal sources reflecting individual cells. The expectation is that such sources should be localized, with a sparse or skewed spatial distribution of pixel weights for each cell, such that most pixels have almost no weight but a few are strongly weighted at the cell’s location. Skewness, a distribution’s third moment normalized by the cube of the standard deviation, provides a means of characterizing sparseness and searching for statistical signatures of individual cells. Similarly, if the intervals between cells’ brief rises in [Ca2+] are greater than their durations, the distributions of signals’ time variations will also be sparse and skewed (Discussion).

These expectations motivated our application of ICA following PCA. ICA is suited to extracting cellular signals since it seeks pairs of spatial filters and time traces that are sparse and statistically independent of one another. In practice, we found cells can exhibit significant signal correlations and ICA will still work well, even for cell pairs with moderately high correlation coefficients. We used a version of the FastICA algorithm that maximizes the sparseness of extracted signals (Hyvarinen and Oja, 2000), which we expressed by a combination of spatial and temporal skewness (Supplemental Experimental Procedures).

ICA Yields Ca2+ Signals That Are Statistically Sparse

We examined the sparseness of Ca2+ signals recorded by two-photon microscopy in the cerebellar vermis of awake, head-restrained mice (Figure 2). We used multi-cell bolus loading of the Ca2+-indicator Oregon Green 488 BAPTA-1-AM to label neurons and astrocytes in the cerebellar molecular layer (Nimmerjahn et al., 2009; Ozden et al., 2008) (Supplemental Experimental Procedures). The mice stood on an exercise ball and could walk or run at liberty during imaging (Dombeck et al., 2007; Nimmerjahn et al., 2009). This permitted recording of the dendritic Ca2+ spikes associated with complex (Na+ and Ca2+) action potentials in Purkinje cells (Flusberg et al., 2008; Ozden et al., 2008). We also observed Ca2+ activation in Bergmann glial fibers (Nimmerjahn et al., 2009). Application of PCA to the data yielded principal components that were generally not spatially localized and failed to isolate cells’ activities (Figures 2A and 2B, top row). Distributions of signal values along the principal component vectors were highly symmetric and approximately Gaussian (Figure 2C, right) and did not separate Ca2+ transients from background noise. ICA transformed these components into new basis vectors with more distinctive properties.

Figure 2
Independent Component Analysis Identifies Sparse, Intracellular Ca2+ Signals

A majority of independent components had spatial filters far more localized than those of principal components and could reasonably represent individual cells (Figures 2A and 2B, bottom row). Spatial filters found by ICA had skewed distributions (4.4 ± 2.7, mean skewness ± s.d., n = 300 independent components in 5 mice, versus −0.009 ± 0.26 for 504 principal components), with the weights of small subsets of pixels up to 19 standard deviations greater than background pixels (Figure 2C, left). Many filters resembled a stripe in the rostral-caudal direction, with the long axis perpendicular to the folium of the cerebellar cortex (Figure 2A). This is the expected optical cross-section through the dendritic tree of a single Purkinje cell, since these cells have nearly planar dendritic trees lying perpendicular to the field of view (Flusberg et al., 2008; Ozden et al., 2008). The stripes’ widths (7.0 ± 2.3 µm, mean ± s.d.; n=199 dendrites in 5 mice) and lengths (90 ± 40 µm) as estimated by twice the standard deviations of Gaussian fits were consistent with values derived anatomically (Llinas et al., 2004; Palay and Chan-Palay, 1974) or in prior in vivo Ca2+ imaging studies of Purkinje cells (Flusberg et al., 2008; Ozden et al., 2008).

In the temporal domain, Ca2+ activity traces of the independent components also had sparse statistics. Signal distributions were asymmetric and non-Gaussian, with sparse sets of time frames during which fluorescence signals rose by up to eight standard deviations (Figure 2C, right). For the striped independent components representing Purkinje cell dendritic trees, the time traces yielded Ca2+ spike rates and waveforms similar to those from prior manual or semi-automated analyses of cerebellar Ca2+ imaging data (Flusberg et al., 2008; Ozden et al., 2008).

After verifying the sparseness of signals found by ICA, we examined their independence by plotting joint distributions of signals from pairs of components, each representing one Purkinje cell. The data of Figure 2D (bottom) is typical of 276 pairs we studied. In most time frames signals were close to background levels. Each cell was active during a subset of time frames, but the subsets for the two cells were distinct, unlike with pairs of principal components (Figure 2D, top). Only a small number of time frames had high signal amplitudes in both cells, consistent with the cells’ activities being statistically independent. We did encounter some cell pairs whose activity was sufficiently correlated that the pair was extracted as a single independent component. We handled these instances in the third stage of our analysis (see below).

Interestingly, the skewness often provided a means for distinguishing components representing individual cells from those representing artifacts, such as due to brain motion. Nearly all independent components in experimental data had much higher spatial skewness than principal components (Figures 2E). Among the independent components those we recognized by eye to be Purkinje cell or Bergmann glial signals, based on our prior studies of these cells (Flusberg et al., 2008; Nimmerjahn et al., 2009), generally had higher temporal skewness than those representing noise or artifacts (Figure 2E). After sorting independent components by their temporal skewness, it was usually quick and straightforward to segregate and reject artifactual components by inspection. Alternatively, signal components could often be isolated via automated clustering of signals according to their skewness (Figures 2E and S1). To be conservative we regularly examined spatial filters and time traces visually before accepting them as cellular signals. When we inspected components with high skewness we found several types of signals in our cerebellar recordings.

Prominent signals extracted by ICA were those from one, sometimes two, and occasionally three or more of the striped regions that we interpreted to be cross-sections of the corresponding number of Purkinje cell dendritic trees (Figures 2A and and5).5). Signals from these stripes exhibited brief increases in fluorescence (<50 ms rise), followed by an approximately exponential decay with time constant 124 ± 63 ms (mean ± s.d., n=150 cells in 5 mice). In awake mice these events occurred at a spontaneous rate of 0.76 ± 0.15 Hz (n=199 cells in 5 mice) when the mice were not moving on the exercise ball. These rates were determined by performing a temporal deconvolution on the time traces to account for the Ca2+-indicator’s kinetics, followed by a threshold operation for spike detection (Figure 1B) (Yaksi and Friedrich, 2006). The rates are consistent with those from physiological recordings of complex spiking (Lang et al., 1999; Servais et al., 2004) and Ca2+ imaging studies of Purkinje cells (Flusberg et al., 2008; Gobel and Helmchen, 2007b; Ozden et al., 2008). Through simultaneous optical and single-unit electrophysiological recordings we verified that Ca2+ spikes found by our procedure corresponded to complex spikes (Figures 5C and S2). ICA also regularly yielded signals with slower dynamics consistent with Ca2+ activation in fibers of Bergmann glia (Nimmerjahn et al., 2009), the sole type of astrocyte in the cerebellar molecular layer (Figure 5). Independent components with skewness values lower than those for Bergmann glia or Purkinje cells generally resembled artifacts in having diffuse spatial weighting, strong correlations with brain movement, and kinetics too fast to be consistent with those of the Ca2+ indicator (Figure S6). We excluded such components from further analyses (Figure 2E).

Figure 5
Automated Cell Sorting Identifies Neuronal and Glial Ca2+ Dynamics from Large-Scale Two-photon Imaging Data

Combining Spatial and Temporal Information Can Improve Cell Sorting

After obtaining these results, we sought to fine tune ICA’s ability to isolate cells. Most prior usages of ICA in brain imaging have maximized spatial sparseness (Reidl et al., 2007), but it is reasonable to consider also temporal sparseness. We did this using spatio-temporal ICA, which optimizes a linear combination of spatial and temporal skewness (Stone et al., 2002). One parameter, µ, set the relative weight of the two. We sought µ values that improved estimation accuracies (Figure S3). To explore, we created artificial movies that mimicked our recordings from the cerebellum and contained signals from Purkinje cells and Bergmann glia. We included photon shot noise, the main noise source in two-photon microscopy, and background structures such as blood vessels to resemble those in the experiments. We defined the fidelity of extracted signals, F, as the correlation coefficient between each cell’s actual dynamics and its reconstructed signal, with F = 100% implying a perfect reconstruction.

Distributions of F as a function of µ depended on multiple parameters, including the field of view size and thus the number of cells, the numbers of pixels and time frames, cells’ mean spike rate relative to the frame rate, and the amplitudes of cells’ signals above background. In nearly all situations representative of our experiments, spatial ICA (µ = 0) was superior to temporal ICA (µ = 1), leading to higher median fidelities and components easily recognizable as Purkinje cells. Temporal ICA often failed to yield components recognizable as cells. Spatio-temporal ICA with µ < 0.5 nearly always led to the highest fidelities, although the gains over a purely spatial ICA were only weakly sensitive to µ and modest, with ~1–10% improvements in median fidelity and ~0–15% improvements in fidelities for individual cells (Figure S3). An exception was that if the frame rate was much higher than the spike rates, the time traces became very sparse and a temporal weighted ICA (µ ≈ 1) yielded the highest fidelities. Tuning µ yielded the most benefit in challenging cases with substantial mixing of cells’ signals in individual pixels. With real data we found by exploration that spatio-temporal ICA (µ ≈ 0.1–0.2) extracted the most components resembling Purkinje cells, so we habitually used this approach.

Comparison of Independent Component and Region of Interest Analyses

To compare ICA and ROI analyses we used a form of ROI analysis that is the best a human analyst could do if she correctly identified sets of example movie frames during which each cell is active. The most challenging situations in our simulations and experiments involved cells with overlapping pixel sets (Figures 3A and and5A)5A) or pixels with signals from cells and neuropil (Figure S4). In such cases ROI analysis had difficulty removing cross talk (Figure 3B and S4). With spatio-temporal ICA cross talk was much reduced, since independent components had negatively weighted surround regions in the spatial filters that subtracted signals from overlapping cells (Figures 3C and S4).

Figure 3
Automated Cell Sorting Outperforms Region of Interest (ROI) Analysis

We studied how signal amplitudes and the field of view area affected signal extraction (Figure 3D). For each pixel photon counts obeyed Poisson statistics, with the mean intensity set by a signal to noise ratio parameter expressing signals’ dynamic range compared to background fluorescence levels (Supplemental Materials). In simulations with a fixed field of view (0.09 mm2; ~100 cells) ICA used increases in signal to noise ratio to improve reconstruction fidelities and reduce cross talk between cells (Figure 3E). ICA’s performance approached an optimum for signal to noise ratios > 0.3, at which the median fidelity approached 95% and >80% of extracted signals had F > 75%. This fidelity was close to the theoretical limit set by a linear regression analysis, which defines the best achievable by any linear combination of principal components (Figure 3D and Supplemental Material). By comparison, ROI analysis did not improve much with increases in signal to noise ratio and failed to separate overlapping cells (Figure 3E). Alternatively, when we distributed a fixed number of pixels across variable field of view areas, ICA achieved high fidelities and low cross talk across a broad range of field sizes, whereas ROI analysis steadily degraded as increased overlap between cells led to greater cross talk (Figure 3F). With the largest fields of view both methods failed, yielding fidelities scarcely better than the cross talk values. Overall, ICA was superior to ROI analysis and much closer to performance limits set by linear regression (Figure 3D).

Image Segmentation for Separating Correlated Signals

An advantage of imaging is its ability to sample dense networks of cells, since the dynamical correlations between neighboring cells are often of prime interest. A key question is whether ICA, which relies on statistical independence to identify cells, is limited in its ability to separate cells with correlated signals?

In simulations with varying degrees of pairwise Purkinje cell synchrony, we found ICA could readily distinguish cells whose spike trains had correlation coefficients, r, as high as 0.8 at the spike (≤ 1 Hz) and frame (10 Hz) rates simulated. The correlated cells were far from independent, but sorting performed well, yielding high fidelity estimates comparable to those attained without correlations (r=0). This was because activity traces of individual cells were still more skew than those from cell mixtures, consistent with prior reports ICA is often robust to deviations from the underlying model assumptions (Hyvarinen, 1999). Still, when r > 0.8, ICA often extracted signals from strongly correlated cells in a single independent component.

Our sorting procedure thus augments ICA with an image segmentation step to disentangle signals from highly correlated cells that are spatially separated (Figures 1B and and4).4). Following ICA, we smoothed each component’s spatial filter and applied a binary threshold to find local regions with strong signal contributions. If a filter contained more than one such region, we created new filters, each of which contained only one of the image segments. These steps separated distinct cells with strongly correlated activity (Figure 4). After these tests on simulated data, we studied data from the cerebellar cortex of live mice.

Figure 4
Image Segmentation Improves Extraction of Signals from Correlated Cells

Sorting Cerebellar Ca2+ Signals From Live Mice

To illustrate cell sorting in an experimental context, we studied data from the molecular layer of cerebellar lobules V and VI of anesthetized and awake head-fixed mice. Using spatio-temporal ICA with µ = 0.1–0.2 followed by image segmentation, we extracted filters with shapes of Purkinje cell dendrites that showed spontaneous (Figures 5A and 5B) and evoked Ca2+-spiking activity (Figures 6 and S5).

Figure 6
Ca2+ Spiking in Purkinje Cells of the Cerebellar Vermis Depends on Behavioral State

There were also filters with more isotropic profiles and slower dynamics representing Ca2+ activation of Bergmann glial fibers (Figures 5A and 5B) (Nimmerjahn et al., 2009). Since Bergmann glial fibers entwine Purkinje cell dendrites (Grosche et al., 1999), many pixels contained the activity of both cell types. Thus, it is challenging to separate Purkinje cell and Bergmann glial signals cleanly by ROI analysis. Yet, by automated sorting we disentangled Purkinje cell Ca2+ spikes (Figure 5B, top four traces) from Bergmann glial Ca2+-transients (Figure 5B, bottom four traces) without noticeable cross talk. Spontaneous glial signals found by cell sorting covered ellipsoidal areas and had event rates much lower than neuronal Ca2+ spike rates in both awake and anesthetized mice. The time course of glial Ca2+ activation conformed closely (r = 0.9 ± 0.1, s.d.; n = 96 events in 11 mice) to a double exponential function with similar time constants for the rise (1.5 ± 0.7 s) and decay (1.8 ± 1.2 s). These kinetics resembled those of Ca2+ ‘bursts’ seen previously in Bergmann glia in cerebellar slices (Beierlein and Regehr, 2006; Piet and Jahr, 2007), anesthetized (Hoogland et al., 2009) and awake mice (Nimmerjahn et al., 2009).

After extracting Purkinje cells’ activities, we estimated the cells’ Ca2+ spike trains in binary format using a deconvolution and threshold approach (Yaksi and Friedrich, 2006) (Figure 1B). To quantify spike detection accuracies, we combined two-photon microscopy with simultaneous extracellular single unit electrical recordings of Purkinje cell activity in anesthetized mice (Figure 5C). On the assumption the electrophysiological traces yielded perfect records of complex spiking, we tested our ability to extract complex spikes from the optical data. (This assumption is in general false, as imperfect electrical traces could lead to underestimates of the accuracy of optical spike detection). We used receiver operating characteristic (ROC) analysis, which compares probabilities of correct and incorrect spike identification (Figure S2) (Fawcett, 2006).

These probabilities depend on the spike detection threshold, and the ROC curve summarizes the tradeoff between sensitivity and accuracy in a two-dimensional plot (Figure S2). The area under this curve equals the probability that an ideal classification algorithm would correctly discriminate a randomly selected time frame with a spike from one without a spike (Fawcett, 2006), a measure not dependent on the spike threshold. In our data, areas under the ROC curves were 0.84 ± 0.06 (mean ± s.d.; n = 7 cells) for image segments and 0.92 ± 0.05 for raw ICA signals. This implies ~85–90% discrimination accuracy. False positive and false negative spike detection rates (Figure S2) were comparable to those in recent imaging studies (Ozden et al., 2008; Sasaki et al., 2008) and tetrode recordings (Harris et al., 2000).

Following these validation studies in anesthetized mice, we studied awake mice and extracted up to ~100 Purkinje cells from fields up to ~500 µm wide (Figures 5D and 5E). Our algorithm extracted spike trains from cells tiling the entire field of view, many of which were closely adjacent and extremely difficult to separate by manual methods. Automated cell sorting also separated data components that seemed to represent movement artifacts (Figure S6), similarly to ICA’s isolation of movement artifacts in fMRI studies (Beckmann and Smith, 2004; McKeown et al., 1998). This meant our estimates of cellular activity were relatively uncorrupted by animal motion, showing that ICA can complement image registration techniques for removal of motion artifacts. As a test of ICA’s robustness, we compared cells identified across an entire data set to those identified when only periods of the mouse running were used for analysis. The latter analysis identified ~50% (range, 20–74%; 5 mice) of the cells from the full data set, but these cells appeared to be correctly identified since they were found in both cases. This test is extreme, for it assumes an entire experiment involves continuous running, and illustrates ICA’s ability to find cells despite the presence of motion artifacts.

Locomotor Behavior Increases Purkinje Cells’ Complex Spike Rates

A longstanding goal has been to determine how activity in the olivo-cerebellar circuit is modulated during motor behavior. By combining automated cell sorting and two-photon imaging in behaving mice we examined relationships between Purkinje cell complex spiking and locomotion.

Using automated sorting we extracted Ca2+ signals from Purkinje cells in cerebellar lobules V and VI in head-restrained mice allowed to run voluntarily on an exercise ball. Mean rates of complex spiking varied between periods of anesthesia, alert rest, and active locomotion for each mouse (Figure 6). For individual cells studied in all three conditions, spike rates rose from 0.48 ± 0.27 Hz (mean ± s.d., n = 199 cells in 5 mice) under isoflurane anesthesia, to 0.76 ± 0.15 in alert but resting mice, to 1.0 ± 0.18 Hz in awake mice during active locomotion (Figures 6C and 6D) (p<0.001 for all pairwise comparisons; Wilcoxon signed rank test). Thus, gross rates of complex spiking depended on behavioral state, consistent with recent optical studies (Flusberg et al., 2008). Prior electrophysiological studies of locomotion in decerebrated cats have been ambiguous on this point (Andersson and Armstrong, 1987).

In mice studied in both awake and isoflurane-anesthetized conditions, we occasionally saw adjoining groups of Purkinje cell dendritic trees that were identified in the full data set but had little or no complex spiking (rate < 0.01 Hz) during anesthesia. For example, in Figure 6B dendritic trees labeled 1–8 and 35–42 by medio-lateral position were largely silent under anesthesia. This was not so for all cells, as shown by dendrites 9–34 that spiked at 0.38 ± 0.15 Hz (n=25 dendrites). After cessation of anesthesia all Purkinje cells (1–42) in view resumed Ca2+ spiking (Figure 6B). This example highlights the combined utility of automated sorting and Ca2+ imaging by revealing phenomena in dense cellular networks inaccessible to electrical recordings.

Cerebellar Microzones Remain Anatomically Stable Across Behavioral States

Our studies of the vermis in behaving mice allowed us to reexamine the organization of parasagittal bands of correlated Purkinje cells noted previously by Ca2+ imaging in anesthetized animals (Ozden et al., 2008) and electrophysiological means (Andersson and Oscarsson, 1978; Lang et al., 1999). We examined if microzones have stable anatomical boundaries (Andersson and Oscarsson, 1978) or represent flexible ensembles that vary across behavioral states (Welsh et al., 1995).

By studying pairwise correlation coefficients for Ca2+-related fluorescence signals, or for the corresponding spike trains, we repeatedly observed enhanced correlations for multiple, closely situated pairs of Purkinje cell dendrites as compared to more distally separated pairs (Figures 7A and 7B). We looked for the anatomical boundaries of such microzones of highly correlated cells by clustering Ca2+-spike trains using pairwise correlation coefficients (Figure 7C and 7D) (Ozden et al., 2008). In alert but resting mice, cluster analysis partitioned cells into microzones with higher correlations for intra-zone pairs (r = 0.10 ± 0.08 mean ± s.d.; n=1418 pairs in 5 mice) than inter-zone pairs (0.02 ± 0.03, n = 2474; p<0.001 Wilcoxon rank sum test). Strikingly, the boundaries between microzones were sharply delineated, with the spatial transition between microzones generally occurring in one cell width rather than in a gradual manner over multiple Purkinje cell dendritic trees.

Figure 7
Cerebellar Microzones Exhibit Changes in Correlated Activity but Stable Anatomical Organization Across Different Behavioral States

Like spike rates, pairwise correlations between Ca2+ spike trains varied between anesthetized, alert but resting, and actively moving states. The correlations we saw among cells in each microzone grew stronger during active movement (p<0.001 Wilcoxon signed rank test) for intra-zone cell pairs (r = 0.20 ± 0.09), and to a statistically significant but far lesser extent for inter-zone pairs (r = 0.03 ± 0.05), as compared to alert rest. When considered together with the rises in spike rates across the field of view, this distinction in synchrony between intra- and inter-zone cell pairs shows there are different degrees of correlated input to intra-zone versus inter-zone pairs and that the rise in synchrony is likely not just due to increased spike rates in independent cells. Likewise, it would be hard to argue the rise in measured spike rates during locomotion was due to motion artifacts, since motion artifacts would not lead to precisely defined microzones of high pairwise synchrony.

Compared to rest or locomotion, isoflurane anesthesia reduced correlations dramatically (r = 0.03 ± 0.06 intra-zone; r = 0.001 ± 0.02 inter-zone). In each microzone nearly all dendrite pairs were significantly correlated (p<0.01, likelihood ratio test) in resting (86% of pairs) and actively moving mice (95%), but only 26% of intrazone pairs were significantly correlated under anesthesia. By comparison, less than a third of dendrite pairs from different microzones were significantly correlated in mice at rest (29%), during movement (33%) and under isoflurane anesthesia (7%). Taken together, these results show both Ca2+ spike rates and pairwise synchrony within microzones increased during active movement as compared to alert rest or anesthesia.

The organization of correlated Purkinje cell complex spiking was earlier proposed to be dynamically modulated during motor behavior (Welsh et al., 1995). We tested the temporal stability of microzones in two ways. First, we divided each experiment into 60 s epochs and performed cluster analysis separately for each epoch. We found each Purkinje cell was assigned to the same microzone during 96 ± 6% of epochs (n = 44 epochs). Second, we tested if microzones changed their organization across different behaviors. By comparing microzones found during locomotion and during alert rest, we found 98.5 ± 0.8% (mean ± 68% confidence interval; n = 199 cells in 5 mice) of cells fell in the same microzone in the two conditions (Figure 7D). Our results did not reveal a modulation of microzone boundaries.


Large-scale Ca2+ imaging studies pose analysis challenges of three main categories: identification of cells’ locations, extraction of Ca2+ signals, and detection of neuronal spikes. Only the third problem has received much attention, and several methods exist for detecting spikes in fluorescence traces using temporal deconvolution (Yaksi and Friedrich, 2006), template matching (Greenberg et al., 2008; Kerr et al., 2005), particle filters (Vogelstein et al., 2009) or machine learning (Sasaki et al., 2008). However, these algorithms rely on the assumption the first two challenges have already been addressed, yielding a paired spatial filter and time trace for each cell. To attain these pairs, we developed automated procedures based on general principles that permit a decomposition of data into constituent independent signals.

Growing Need for Automated Cell Sorting

Ca2+ imaging data has commonly been analyzed by manual identification of cell bodies based on their morphologies in static fluorescence images (Dombeck et al., 2007; Gobel et al., 2007; Greenberg et al., 2008). Some studies have used semi-automated methods that rely on morphological filters to find cellular boundaries (Ohki et al., 2005). After finding structures with the sizes and shapes expected of cell bodies, these procedures define ROIs over which the fluorescence is averaged to extract each cell’s dynamics. With the most commonly used AM-ester conjugated Ca2+ indicators this approach generally fails to identify neuronal dendrites or fine glial processes, since these do not stand out with high contrast and cannot be readily delineated by morphological filtering. In areas such as neocortex in which the neuropil displays Ca2+ activity (Kerr et al., 2005) that inherently contaminates somatic Ca2+ signals (Gobel and Helmchen, 2007a; Kerr and Denk, 2008), ROI and morphological analyses can have difficulty excluding neuropil signals from the cellular activity traces (Figure S4). For studies of cells’ receptive fields or sensory tuning curves, cross talk from neuropil has the potential to mask sharply tuned responses, since neuropil can exhibit untuned or broadly tuned signals (Ohki et al., 2005). Given these challenges, sorting approaches based on signal statistics are warranted.

A recent study extracted Ca2+ signals from Purkinje cell dendrites by a method using temporal cross-correlations to find sets of pixels contributing to each cell’s signal (Ozden et al., 2008). A user selects an area slightly larger than each dendrite and computes the cross-correlations among all pixels in this region. The final ROI contains all pixels highly correlated with many others in the selected area. This method used signal statistics to localize cells but required 2–3 hours of manual work for each data set. As the number of cells in Ca2+ imaging studies extends into the hundreds (Gobel et al., 2007; Ohki et al., 2005) or beyond, human selection of pixels will become increasingly prohibitive. Our procedure takes only a few minutes of user supervision to input the number of principal components and screen the results. We habitually scanned the independent components by eye, but for most data sets the selection of independent components representing cells could be automated by cluster analyses (Figures S1 and S6), especially with anesthetized mice, which had lower levels of brain displacement than behaving mice. To be cautious, users should compare the results of automated cell sorting to other data, such as from electrophysiological (Figure 5) or anatomical analyses (Nimmerjahn et al., 2009). We also recommend an initial, systematic testing of a range of µ values, to optimize the ability of spatio-temporal ICA to find cells.

Our use of ICA builds on its prior usage for analysis of optical data of other types, such as for extraction of voltage signals acquired in sea slugs by a photodiode array (Brown et al., 2001). Two studies have used PCA followed by ICA to find functional domains and hemodynamic signatures in optical imaging data (Reidl et al., 2007; Siegel et al., 2007). Signals came from large regions and so often only a few (e.g. ~6) principal components were retained (Reidl et al., 2007). ICA has been used several times to analyze human brain activity (Beckmann and Smith, 2004; Guimaraes et al., 2007; Makeig et al., 1997; McKeown et al., 1998).

The approach here builds on and differs from previous optical studies by combining spatial and temporal statistics and by following ICA with image segmentation. We preceded ICA with PCA, to reduce the dimensionality of the data and to help ICA find global optima. PCA is not the only means of dimensional reduction, and other approaches, such as dividing the field of view into subsets each of which is larger than individual cells, might provide viable alternatives. Our overall procedure is grounded in three suppositions: (1) cellular signals are mathematically separable into products of paired spatial and temporal components; (2) signals from different cells are statistically independent; (3) cells’ spatial filters and temporal signals have skewed distributions. Interestingly, our procedure proved effective under conditions with modest deviations from these assumptions.

Cell Sorting Separates Data Into Spatial and Temporal Components

The supposition that cellular signals are separable into spatial filters and time courses is based on the observation neuronal Ca2+ signals in large-scale imaging data generally arise from fixed locations and do not convey details at the shortest physiological time scales regarding intracellular propagation of [Ca2+] changes. For example, for Purkinje cell Ca2+ spikes any delay between Ca2+ activation in different cell parts is generally briefer than one image frame (50–100 ms), permitting a separation of space and time in describing these events. Astrocytic Ca2+ waves that propagate far more slowly than neuronal Ca2+ spikes violate the assumption of separability (Fiacco and McCarthy, 2006). Still, our procedure extracted a useful approximation of Ca2+ waves that expanded over a fraction of the field of view (Figures 5A and 5B). This suggests ICA remains a useful tool for analyzing weakly non-separable signals. For forms of glial Ca2+ activation that propagate across long distances (Nimmerjahn et al., 2009) other analyses seem more suitable.

Spatio-temporal separability may also be violated due to movement artifacts. Still, ICA can often separate components representing motion artifacts from physiological signals (Figure S6). This agrees with analyses of fMRI data showing ICA can remove moderate levels of subject motion (McKeown et al., 1998; Reidl et al., 2007). ICA cannot rescue data badly corrupted by motion. When motion artifacts were present at an intermediate level, the skewness of components representing motion were sometimes comparable to those representing cells, but motion components could still be identified based on their having kinetics inconsistent with those of the Ca2+-indicator (Figure S6).

Automated Cell Sorting is Robust to Correlations in Cells’ Activities

For cell pairs with modest correlations (r < 0.8), ICA was able to identify the individual cells. Since no approach to cell sorting can separate completely synchronized cells without considering geometric information, we used image segmentation following ICA. The result is a robust, hybrid procedure that handled strong levels of correlation (r ~ 0.9) in simulations and separated highly correlated dendrites in our cerebellar data that clearly belonged to different Purkinje cells based on their anatomical separation (Figure 4). High levels of complex spike synchrony are known to arise for distinct Purkinje cells with inputs from the same climbing fiber (Sugihara, 2005). Overall, our method’s ability to find individual cells in cases of weak or strong synchrony is a key aspect of the procedure’s utility.

Cell Sorting Based on Statistical Skewness

An assumption of spatial skewness is valid when individual cells occupy only a small fraction of the pixels in the field of view. In our studies of Purkinje cells, an assumption of skew temporal statistics was also satisfied, since the mean time between Ca2+ spikes was greater than the individual spikes’ durations. However, sparse activation in time is not the only means of achieving a skewed distribution of signal amplitudes. Cells with high rates of activity that are strongly modulated in time, such as during bursts, could also have skewed distributions of fluorescence amplitudes. Thus, our approach should be well suited to neurons that exhibit irregular spiking, such as high-frequency bursts interrupted by periods of quiescence (Dombeck et al., 2007; Greenberg et al., 2008; Margrie et al., 2002; Orger et al., 2008). Both PCA and ICA ignore cells with very low activity levels, so our method fails to identify cells that are totally inactive in the data. This could lead to underestimation of cell densities and overestimation of mean activity levels in areas where many cells are largely silent (Greenberg et al., 2008). Small fields of view aided the identification of cells with low activity levels (Figure S4), so with such cells it might be beneficial to reduce the dimensionality of the raw data by sub-dividing the field of view, rather than by PCA.

High rates of tonic, regular Ca2+ activation will also lead to signal distributions that are not skew, potentially leading ICA to ignore such cells. In our recordings we had difficulty extracting cerebellar interneurons (Sullivan et al., 2005), since these cells have high spontaneous spike rates compared to the image acquisition rate and do not show bursty Ca2+ dynamics. A purely spatial ICA combined with image segmentation might be better suited to extract these cells. Alternatively, a morphological analysis might work at the risk of increasing cross talk.

Cerebellar Microzones As Stable Anatomical Entities

Automated cell sorting allowed us to re-examine a debate on the organization of Purkinje cells’ complex spiking activity. One view holds there are flexible assemblies in the cerebellar cortex that alter their composition of Purkinje cells with synchronous complex spikes according to changing behavioral requirements (Llinas, 1991). Microelectrode array recordings of complex spiking in behaving rats suggested that different groups of Purkinje cells were synchronously activated at different phases of movement (Welsh et al., 1995). An opposing view holds cerebellar microzones provide a stable architecture for controlling different parts of the body and are defined by the anatomical organization of the climbing fibers that drive the complex spikes (Apps and Garwicz, 2005). Mapping studies have shown strips of neighboring Purkinje cells receive climbing fiber inputs with similar cutaneous receptive fields (Andersson and Oscarsson, 1978; Jorntell et al., 2000), and that these maps are reproducible across subjects (Ekerot et al., 1991). These data did not adjudicate whether adjacent microzones are precisely demarcated from one another or merge smoothly.

By imaging the Ca2+ spikes that represent the Ca2+ component of Purkinje cells’ complex (Na+ and Ca2+) spikes (Figure 5) (Flusberg et al., 2008; Ozden et al., 2008), we found microzones’ boundaries are sharply delineated in awake animals, to about the width of one Purkinje cell (Figure 7). Precise microzones were also seen by Ca2+ imaging in anesthetized rodents (Ozden et al., 2008). Our results complement prior studies in awake animals using electrode arrays, which showed complex spiking synchrony exists in bands extending millimeter-sized distances in the rostral-caudal dimension (Lang et al., 1999; Welsh et al., 1995). The 250-µm-spacing between electrodes used in those studies precluded fine resolution of microzone structure in the medio-lateral direction.

By extracting Ca2+ spike trains from large populations of Purkinje cells during locomotion and alert rest, we studied the stability of microzones. Microzone boundaries and cellular compositions were stable across the two behavioral states examined. During locomotion, Ca2+ spike rates rose and levels of synchronous activation increased within microzones but decreased between microzones. Thus, microzones not only retained stable boundaries across different behaviors but also increased in prominence during motor behavior. The discrepancy in synchrony between pairs of cells within and across microzones, as well as the sharpness of microzones’ boundaries, indicates that our results are not due to noise, which would not lead to precisely defined regions of high pairwise synchrony. Our results are consistent with data gathered by high-speed imaging of Purkinje cell Ca2+ spikes in freely behaving mice, which revealed increases in spike rates and pairwise synchrony during locomotion at the level of population statistics (Flusberg et al., 2008).

Our findings support the view that microzones are stable anatomical entities, for we obtained no evidence of their reorganization during motor behavior. Still, we only explored a single motor behavior and others will need to be examined. The evidence for dynamical reconfiguration of Purkinje cell assemblies (Fukuda et al., 2001; Welsh et al., 1995) should not be discounted, since the reported assemblies of cells were from larger brain areas than those studied here. An attractive possibility reconciling the two viewpoints is that correlated assemblies of different microzones, each stably defined, dynamically reconfigure in different behaviors.

Our data showed both pairwise correlations and spike rates were higher in awake than anesthetized animals. These results contrast with recent studies of neocortex. In rat visual cortex, correlations between layer 2/3 neurons fell during periods of activity as compared to rest (Greenberg et al., 2008). Further, ketamine/xylazine anesthesia reduced spike rates but increased correlations in neocortical neurons (Greenberg et al., 2008), which contrasts with our data on Purkinje cell correlations under isoflurane anesthesia. Ketatmine/xylazine anesthetized rats also exhibited significant correlations in Purkinje cell Ca2+ spiking (Ozden et al., 2008), suggesting different anesthetics might have distinct effects on Purkinje cells’ synchrony.

Technological Outlook

Refinement of automated sorting techniques should further increase the utility of optical imaging for studying dense cellular networks and allow cell sorting to assume a comparably important role as in electrophysiology. By introducing an automated method based on broadly applicable principles, we have created a framework for moving beyond heuristic and semi-automated approaches. Future refinements might incorporate a priori knowledge of various types or sparseness measures other than skewness. Other generalizations might add information about sensory stimuli or animal behavior to derive functional characterizations of cellular activity in an automated way.


Automated Cell Sorting Procedures

We analyzed Ca2+ imaging data using ImageJ plug-ins and custom MATLAB routines. Movies were corrected for lateral movement artifacts using TurboReg (Thevenaz et al., 1998). We then applied our cell sorting protocol comprising: (1) PCA for dimensional reduction; (2) spatio-temporal ICA for extraction of Ca2+ signals; (3) image segmentation to separate highly correlated cells; and, in the case of neuronal signals, (4) temporal deconvolution and spike detection to extract spike times. See Supplemental Toolbox.

Artificial Data

We simulated Ca2+ imaging data by combining artificial spike trains and glial transients with spatial filters designed to reflect the size, shape, and density of Bergmann glia and Purkinje cell dendritic trees, as viewed in optical sections in the mouse cerebellar molecular layer. Each simulation had 1,000 time frames at 10 Hz. We added a static image to the dynamic Ca2+ signals to represent background fluorescence from dye-labeled interneurons, as well as unlabeled regions representing blood vessels. The dynamic and background signals defined a noiseless data set. We then introduced Poisson-distributed photon shot noise. See Supplemental Material.

Animal Procedures

Animal procedures were approved by the Stanford Administrative Panel on Laboratory Animal Care. We used 16 male C57Bl/6 wild type mice (5.5–15 weeks old). Each experiment had two surgeries. Several days before recordings a custom metal head plate was attached to the skull with dental acrylic. This allowed habituation of the mice to head restraint while walking on the exercise ball and stable imaging under anesthetized or awake conditions. On the recording day, a craniotomy was opened over the cerebellar vermis or neocortex. Two-photon imaging in head-restrained mice was performed as in (Nimmerjahn et al., 2009) (Supplemental Experimental Procedures).

Two-photon Imaging

We loaded cortical tissue with the fluorescent Ca2+-indicator Oregon Green 488 BAPTA-1-AM (OGB-1-AM; Molecular Probes) as described (Nimmerjahn et al., 2009). We used a custom two-photon microscope equipped with an ultra-short pulsed Ti:sapphire laser tuned to 800 nm. See Supplemental Material for details.

Analysis of Cerebellar Microzones

We analyzed spiking correlations among all Purkinje cells during anesthetized, awake but resting, and actively moving conditions. We used the Pearson correlation coefficient to compare the binary Ca2+ spike trains for each pair of cells. We grouped cells with similar spike trains using k-means clustering (MacKay, 2003).

Supplementary Material



We thank David Profitt and Stanford’s Varian Machine Shop for expert machining, Andrea Lui for programming assistance and help habituating mice, Quoc-Thang Nguyen and David Kleinfeld for providing imaging software and electronics, Akirah Katoh for consultation on electrophysiological recordings of Purkinje cells, and Shaul Hestrin, Aapo Hyvärinen, Christropher Niell, Carlos Portera-Cailliau, Clay Reid, Krishna Shenoy, Haim Sompolinsky, Michael Stryker, and Byron Yu for helpful correspondence and discussions. Our work was supported by grants to MJS from ONR, NSF, NINDS, NIDCD, the UBC NIH Nanomedicine Center, the Stanford Bio-X program, and the Klingenstein, Sloan, and Packard Foundations, postdoctoral fellowships to AN from the Alexander von Humboldt-Foundation and the International Human Frontier Science Program Organization, and an NSF Graduate Fellowship to EAM.


Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.


  • Andersson G, Armstrong DM. Complex spikes in Purkinje cells in the lateral vermis (b zone) of the cat cerebellum during locomotion. J Physiol. 1987;385:107–134. [PubMed]
  • Andersson G, Oscarsson O. Climbing fiber microzones in cerebellar vermis and their projection to different groups of cells in the lateral vestibular nucleus. Exp Brain Res. 1978;32:565–579. [PubMed]
  • Apps R, Garwicz M. Anatomical and physiological foundations of cerebellar information processing. Nat Rev Neurosci. 2005;6:297–311. [PubMed]
  • Batista AP, Santhanam G, Yu BM, Ryu SI, Afshar A, Shenoy KV. Reference frames for reach planning in macaque dorsal premotor cortex. J Neurophysiol. 2007;98:966–983. [PubMed]
  • Beckmann CF, Smith SM. Probabilistic independent component analysis for functional magnetic resonance imaging. IEEE transactions on medical imaging. 2004;23:137–152. [PubMed]
  • Beierlein M, Regehr WG. Brief bursts of parallel fiber activity trigger calcium signals in bergmann glia. J Neurosci. 2006;26:6958–6967. [PubMed]
  • Bell AJ, Sejnowski TJ. An information-maximization approach to blind separation and blind deconvolution. Neural Comput. 1995;7:1129–1159. [PubMed]
  • Brown GD, Yamada S, Sejnowski TJ. Independent component analysis at the neural cocktail party. Trends Neurosci. 2001;24:54–63. [PubMed]
  • Csicsvari J, Hirase H, Czurko A, Buzsaki G. Reliability and state dependence of pyramidal cell-interneuron synapses in the hippocampus: an ensemble approach in the behaving rat. Neuron. 1998;21:179–189. [PubMed]
  • Dombeck DA, Khabbaz AN, Collman F, Adelman TL, Tank DW. Imaging large-scale neural activity with cellular resolution in awake, mobile mice. Neuron. 2007;56:43–57. [PMC free article] [PubMed]
  • Ekerot CF, Garwicz M, Schouenborg J. Topography and nociceptive receptive fields of climbing fibres projecting to the cerebellar anterior lobe in the cat. The Journal of Physiology. 1991;441:257–274. [PubMed]
  • Fawcett T. An introduction to ROC analysis. Pattern Recognition Letters. 2006;27:861–874.
  • Fee MS, Mitra PP, Kleinfeld D. Automatic sorting of multiple unit neuronal signals in the presence of anisotropic and non-Gaussian variability. J Neurosci Methods. 1996;69:175–188. [PubMed]
  • Fiacco T, McCarthy K. Astrocyte calcium elevations: Properties, propagation, and effects on brain signaling. Glia. 2006;54:676–690. [PubMed]
  • Flusberg BA, Nimmerjahn A, Cocker ED, Mukamel EA, Barretto RP, Ko TH, Burns LD, Jung JC, Schnitzer MJ. High-speed, miniaturized fluorescence microscopy in freely moving mice. Nat Methods. 2008;5:935–938. [PMC free article] [PubMed]
  • Fukuda M, Yamamoto T, Llinas R. The isochronic band hypothesis and climbing fibre regulation of motricity: an experimental study. Eur J Neurosci. 2001;13:315–326. [PubMed]
  • Gobel W, Helmchen F. In vivo calcium imaging of neural network function. Physiology (Bethesda) 2007a;22:358–365. [PubMed]
  • Gobel W, Helmchen F. New angles on neuronal dendrites in vivo. J Neurophysiol. 2007b;98:3770–3779. [PubMed]
  • Gobel W, Kampa BM, Helmchen F. Imaging cellular network dynamics in three dimensions using fast 3D laser scanning. Nat Methods. 2007;4:73–79. [PubMed]
  • Greenberg DS, Houweling AR, Kerr JN. Population imaging of ongoing neuronal activity in the visual cortex of awake rats. Nat Neurosci. 2008;11:749–751. [PubMed]
  • Grosche J, Matyash V, Möller T, Verkhratsky A, Reichenbach A, Kettenmann H. Microdomains for neuron-glia interaction: parallel fiber signaling to Bergmann glial cells. Nature Neuroscience. 1999;2:139–143. [PubMed]
  • Guimaraes MP, Wong DK, Uy ET, Grosenick L, Suppes P. Single-trial classification of MEG recordings. IEEE Trans Biomed Eng. 2007;54:436–443. [PubMed]
  • Harris KD, Henze DA, Csicsvari J, Hirase H, Buzsaki G. Accuracy of tetrode spike separation as determined by simultaneous intracellular and extracellular measurements. J Neurophysiol. 2000;84:401–414. [PubMed]
  • Hastie T, Tibshirani R, Friedman JH. The elements of statistical learning: data mining, inference, and prediction. New York: Springer; 2001. p. 22.
  • Helmchen F, Imoto K, Sakmann B. Ca2+ buffering and action potential-evoked Ca2+ signaling in dendrites of pyramidal neurons. Biophys J. 1996;70:1069–1081. [PubMed]
  • Hoogland TM, Kuhn B, Gobel W, Huang W, Nakai J, Helmchen F, Flint J, Wang SS. Radially expanding transglial calcium waves in the intact cerebellum. Proc Natl Acad Sci U S A. 2009;106:3496–3501. [PubMed]
  • Hyvarinen A. Fast and robust fixed-point algorithms for independent component analysis. IEEE Trans Neural Netw. 1999;10:626–634. [PubMed]
  • Hyvarinen A, Oja E. Independent component analysis: algorithms and applications. Neural Netw. 2000;13:411–430. [PubMed]
  • Jorntell H, Ekerot C, Garwicz M, Luo XL. Functional organization of climbing fibre projection to the cerebellar anterior lobe of the rat. The Journal of Physiology. 2000;522(Pt 2):297–309. [PubMed]
  • Kerr JN, Denk W. Imaging in vivo: watching the brain in action. Nat Rev Neurosci. 2008;9:195–205. [PubMed]
  • Kerr JN, Greenberg D, Helmchen F. Imaging input and output of neocortical networks in vivo. Proc Natl Acad Sci U S A. 2005;102:14063–14068. [PubMed]
  • Lang EJ, Sugihara I, Welsh JP, Llinas R. Patterns of spontaneous purkinje cell complex spike activity in the awake rat. J Neurosci. 1999;19:2728–2739. [PubMed]
  • Lewicki MS. A review of methods for spike sorting: the detection and classification of neural action potentials. Network. 1998;9:R53–R78. [PubMed]
  • Llinas R. In: Motor Control: Concepts and Issues. Humphrey DR, Freund H-J, editors. New York: Wiley; 1991. pp. 223–242.
  • Llinas RR, Walton KD, Lang EJ. Cerebellum. In: Shepherd GM, editor. The synaptic organization of the brain. Oxford ; New York: Oxford University Press; 2004. pp. 271–310.
  • MacKay DJC. Information theory, inference, and learning algorithms. Cambridge, UK ; New York: Cambridge University Press; 2003. pp. 284–292.
  • Makeig S, Jung TP, Bell AJ, Ghahremani D, Sejnowski TJ. Blind separation of auditory event-related brain responses into independent components. Proceedings of the National Academy of Sciences of the United States of America. 1997;94:10979–10984. [PubMed]
  • Margrie TW, Brecht M, Sakmann B. In vivo, low-resistance, whole-cell recordings from neurons in the anaesthetized and awake mammalian brain. Pflugers Archiv: European journal of physiology. 2002;444:491–498. [PubMed]
  • McKeown MJ, Makeig S, Brown GG, Jung TP, Kindermann SS, Bell AJ, Sejnowski TJ. Analysis of fMRI data by blind separation into independent spatial components. Hum Brain Mapp. 1998;6:160–188. [PubMed]
  • Meister M. Multineuronal codes in retinal signaling. Proc Natl Acad Sci U S A. 1996;93:609–614. [PubMed]
  • Mitra PP, Pesaran B. Analysis of dynamic brain imaging data. Biophys J. 1999;76:691–708. [PubMed]
  • Mrsic-Flogel TD, Hofer SB, Ohki K, Reid RC, Bonhoeffer T, Hubener M. Homeostatic regulation of eye-specific responses in visual cortex during ocular dominance plasticity. Neuron. 2007;54:961–972. [PubMed]
  • Niell CM, Smith SJ. Functional imaging reveals rapid development of visual response properties in the zebrafish tectum. Neuron. 2005;45:941–951. [PubMed]
  • Nimmerjahn A, Mukamel EA, Schnitzer MJ. Motor behavior activates Bergmann glial networks. Neuron. 2009;62:400–412. [PMC free article] [PubMed]
  • Ohki K, Chung S, Ch'ng YH, Kara P, Reid RC. Functional imaging with cellular resolution reveals precise micro-architecture in visual cortex. Nature. 2005;433:597–603. [PubMed]
  • Orger MB, Kampff AR, Severi KE, Bollmann JH, Engert F. Control of visually guided behavior by distinct populations of spinal projection neurons. Nat Neurosci. 2008;11:327–333. [PMC free article] [PubMed]
  • Ozden I, Lee HM, Sullivan MR, Wang SS. Identification and clustering of event patterns from in vivo multiphoton optical recordings of neuronal ensembles. J Neurophysiol. 2008;100:495–503. [PubMed]
  • Palay SL, Chan-Palay V. Cerebellar cortex: cytology and organization. Berlin, Heidelberg, New York: Springer; 1974. p. 32.
  • Piet R, Jahr CE. Glutamatergic and purinergic receptor-mediated calcium transients in Bergmann glial cells. J Neurosci. 2007;27:4027–4035. [PMC free article] [PubMed]
  • Reidl J, Starke J, Omer DB, Grinvald A, Spors H. Independent component analysis of high-resolution imaging data identifies distinct functional domains. Neuroimage. 2007;34:94–108. [PubMed]
  • Sasaki T, Takahashi N, Matsuki N, Ikegaya Y. Fast and accurate detection of action potentials from somatic calcium fluctuations. Journal of Neurophysiology. 2008;100:1668–1676. [PubMed]
  • Servais L, Bearzatto B, Hourez R, Dan B, Schiffmann SN, Cheron G. Effect of simple spike firing mode on complex spike firing rate and waveform in cerebellar Purkinje cells in non-anesthetized mice. Neuroscience letters. 2004;367:171–176. [PubMed]
  • Siegel RM, Duann JR, Jung TP, Sejnowski T. Spatiotemporal dynamics of the functional architecture for gain fields in inferior parietal lobule of behaving monkey. Cereb Cortex. 2007;17:378–390. [PMC free article] [PubMed]
  • Stone JV, Porrill J, Porter NR, Wilkinson ID. Spatiotemporal independent component analysis of event-related fMRI data using skewed probability density functions. Neuroimage. 2002;15:407–421. [PubMed]
  • Stosiek C, Garaschuk O, Holthoff K, Konnerth A. In vivo two-photon calcium imaging of neuronal networks. Proc Natl Acad Sci U S A. 2003;100:7319–7324. [PubMed]
  • Sugihara I. Microzonal projection and climbing fiber remodeling in single olivocerebellar axons of newborn rats at postnatal days 4–7. J Comp Neurol. 2005;487:93–106. [PubMed]
  • Sullivan MR, Nimmerjahn A, Sarkisov DV, Helmchen F, Wang SS. In vivo calcium imaging of circuit activity in cerebellar cortex. J Neurophysiol. 2005;94:1636–1644. [PubMed]
  • Thevenaz P, Ruttimann UE, Unser M. A pyramid approach to subpixel registration based on intensity. IEEE Trans Image Process. 1998;7:27–41. [PubMed]
  • Vogelstein JT, Watson BO, Packer AM, Yuste R, Jedynak B, Paninski L. Spike inference from calcium imaging using sequential Monte Carlo methods. Biophys J. 2009;97:636–655. [PubMed]
  • Welsh JP, Lang EJ, Suglhara I, Llinas R. Dynamic organization of motor control within the olivocerebellar system. Nature. 1995;374:453–457. [PubMed]
  • Yaksi E, Friedrich RW. Reconstruction of firing rate changes across neuronal populations by temporally deconvolved Ca2+ imaging. Nat Methods. 2006;3:377–383. [PubMed]