Search tips
Search criteria 


Logo of narLink to Publisher's site
Nucleic Acids Res. 1979 September 11; 7(1): 179–192.
PMCID: PMC328004

Site specific enzymatic cleavage of RNA.


The hybridization of a DNA oligonucleotide a specific tetramer or longer) will direct a cleavage by RNase H (EC to a specific site in RNA. The resulting fragments can then be labeled at their 5' or 3' ends, purified, and sequenced directly. This procedure is demonstrated with two RNA molecules of known sequence: 5.8S rRNA from yeast (158 nucleotides) and satellite tobacco necrosis virus (STNV) RNA (1240 nucleotides).

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.5M), or click on a page image below to browse page by page.

Images in this article

Click on the image to see a larger version.

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press