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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Neurobiol Aging. Author manuscript; available in PMC Apr 1, 2013.
Published in final edited form as:
PMCID: PMC3279603
NIHMSID: NIHMS334807
DJ-1 and αSYN in LRRK2 CSF do not correlate with striatal dopaminergic function
Min Shi,a* Amy Furay,a* Vesna Sossi,b Jan O. Aasly,c Jeff Armaly,a Yu Wang,ad Zbigniew K. Wszolek,e Ryan J. Uitti,e Kazuko Hasegawa,f Teruo Yokoyama,f Cyrus P. Zabetian,ghi James B. Leverenz,hijk A. Jon Stoessl,l and Jing Zhanga
aDepartment of Pathology, University of Washington School of Medicine, Seattle, WA, USA
bDepartment of Physics & Astronomy, University of British Columbia, Vancouver Hospital and Health Sciences Centre, Purdy Pavilion, Vancouver, BC V6T2B5 Canada
cDepartment of Neurology, St. Olavs Hospital, Trondheim, Norway
dDepartment of Neurosurgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
eDepartment of Neurology, Mayo Clinic Florida, Jacksonville, FL, USA
fDepartment of Neurology, National Hospital Organization, Sagamihara National Hospital, Kanagawa, Japan
gGeriatric Research, Education, and Clinical Center, Veterans Affairs Puget Sound Health Care System, Seattle, WA, USA
hParkinson’s Disease Research, Education, and Clinical Center, Veterans Affairs Puget Sound Health Care System, Seattle, WA, USA
iDepartment of Neurology, University of Washington School of Medicine, Seattle, WA, USA
jDepartment of Psychiatry and Behavioral Sciences, University of Washington School of Medicine, Seattle, WA, USA
kMental Illness Research, Education, and Clinical Center, Veterans Affairs Puget Sound Health Care System, Seattle, WA, USA
lPacific Parkinson’s Research Centre, University of British Columbia & Vancouver Coastal Health, Vancouver, BC V6T2B5 Canada
Address correspondence to: Jing Zhang, Department of Pathology, University of Washington School of Medicine, HMC Box 359635, 325 9th Ave, Seattle, WA 98104, USA. Phone: 1-206-897-5245, Fax: 1-206-897-5452, zhangj/at/uw.edu
*The authors have contributed equally.
Previous studies demonstrated decreased levels of DJ-1 and α-synuclein (αSYN) in human cerebrospinal fluid (CSF) in patients with Parkinson’s disease (PD), but neither marker correlated with PD severity, raising the possibility that they may be excellent progression markers during early or preclinical phases of PD. Individuals carrying the leucine-rich repeat kinase 2 (LRRK2) gene mutation are at increased risk for PD, and the phenotype of LRRK2 patients is almost identical to sporadic PD. To determine whether dopaminergic dysfunction in the basal ganglia, as determined by positron emission tomography (PET) scans, correlates with CSF levels of DJ-1 and αSYN during preclinical stages, Luminex assays were used to analyze CSF samples from asymptomatic LRRK2 mutation carriers, along with carriers who presented with a clinical diagnosis of PD. The data revealed no statistically significant relationship between PET scan evidence of loss of striatal dopaminergic function and the CSF biomarkers DJ-1 and αSYN, except for a weak correlation between DJ-1 and MP binding, suggesting that the use of these potential biomarkers on their own to screen LRRK2 gene mutation carriers for PD is not appropriate.
Keywords: Parkinson’s Disease, LRRK2, gene mutation, biomarker, DJ-1, α-synuclein
A majority of dopaminergic cell bodies in the substantia nigra are lost before clinical symptoms of PD are evident, so there is an urgent need for preclinical biomarkers that correlate with dopaminergic cell loss and other PD pathology. Previously, we and others reported decreased levels of DJ-1 and α-synuclein (αSYN) in human cerebrospinal fluid (CSF) in several large cohorts of PD patients. However, there was no statistically significant relationship between biomarker levels and PD severity in sporadic PD patients (Hong et al., 2010), suggesting that there might be a “floor” effect and these biomarkers may alter more significantly during early stages of the disease, i.e. correlate with early PD progression. Developing biomarkers that predict early phase disease onset and progression may help in the development of treatments designed to slow disease progression and enable treatment when the disease is most responsive to therapy. Since carriers of a mutated gene encoding leucine-rich repeat kinase 2 (LRRK2) are at increased risk for PD (Adams et al., 2005; Cookson, 2010), they are an ideal population for studying disease onset and progression.
Symptomatic (n=8) or asymptomatic (n=18) individuals from Japan, the United States, and Norway carrying the LRRK2 mutated gene were included in this study; all had standard informed consent, clinical examination, and tests. Subjects were PET scanned with three different radiolabeled tracers (18F-6-fluoro-L-dopa, 11C-(±)-α-dihydrotetrabenazine, and 11C –d-threo-methylphenidate) to determine the uptake and decarboxylation of levodopa in the striatum, as well as vesicular monoamine and plasmalemmal dopamine transporter binding. CSF samples were collected via lumbar puncture; Luminex assays measuring αSYN and DJ-1 were used to analyze biomarkers. Statistical analysis was performed using PASW Statistics software (SPSS Inc, Chicago, IL, USA). Correlation between CSF αSYN and DJ-1 levels and PET measurements was evaluated using Kendall’s rank correlation coefficient; overall group differences in CSF biomarkers were analyzed using Mann-Whitney Wilcoxon rank sum tests. For more detail, see the Supplemental Methods section.
Consistent with previous studies, abnormal (decreased) PET changes were observed in asymptomatic and symptomatic subjects (Adams et al., 2005). Since previous reports suggest that blood contamination of CSF (indexed by CSF hemoglobin levels) affects αSYN and DJ-1 levels (Hong et al., 2010), three subjects (2 w/PD, 1 asymptomatic) with hemoglobin levels >250 ng/mL were eliminated from further analysis. As graphically represented in Supplemental Figure 1 (DJ-1 only) and summarized in Supplemental Tables 1 and 2 (DJ-1 and αSYN), there was a slight trend toward a positive correlation between CSF DJ-1 and PET measurements, particularly DTBZ and MP. Next, to determine if such a relationship existed in carriers of the G2019S mutation, the most common form of LRRK2 genetic mutation, we analyzed only G2019S mutation carriers (3 w/PD, 8 asymptomatic) but found no relationship between PET scan results and CSF biomarkers, except for a weak correlation between DJ-1 and MP. Consistent with our previous study in sporadic PD patients (Hong et al., 2010), there was no significant correlation between DJ-1 or αSYN levels in CSF and PD severity (UPDRS motor scores and H&Y stage); there were also no differences between groups in either potential biomarker, probably due to limited case numbers (see Supplemental Data section).
The purpose of this study was to determine if CSF levels of αSYN and DJ-1 are suitable preclinical biomarkers for detecting early phase PD by correlating them with PET scan data measuring progressive loss of striatal dopaminergic function in primarily asymptomatic LRRK2 mutation carriers. These data indicate that CSF αSYN and DJ-1 are not significantly correlated with dopamine dysfunction/cell loss in all LRRK2 gene mutation carriers combined and only a weak correlation was found in the G2019S subset of carriers alone. Although previous studies have suggested that the total levels of αSYN and DJ-1 in CSF are potential diagnostic biomarkers for sporadic PD, with relatively high sensitivity and specificity (Hong et al., 2010), the results of the present study suggest that on their own, they may not be appropriate biomarkers for preclinical or early phase PD diagnosis and/or disease progression, at least in the population of LRRK2 gene mutation carriers, which might be different from typical sporadic PD patients. Although the sample size is small, the present data set strongly suggest that efforts should be directed toward identifying other potential biomarkers, including specific αSYN and DJ-1 species/isoforms, with more robust predicative validity.
Supplementary Material
01
Acknowledgments
The authors would like to thank patients for their generous participation and sample donations. We thank Sydney Thomas and Carmen Ginghina for research coordinating and CSF sample handling, Joshua Bradner for statistical analysis, and Jennifer Lash for her valuable assistance making the arrangements for some research subjects involved in this study for their travel to Seattle, WA and Vancouver, BC.
This research was supported by the NIH: ES004696-5897, P30ES007033-6364, ES016873, and ES019277 (J.Z.); NIEHS: NS060252 (J.Z.), P50NS062684 (C.P.Z., J.B.L. and J.Z.), NS065070 (C.P.Z.), NS057567 (Z.K.W., R.J.U., and J.Z.), and P50NS072187 (Morris K. Udall Center of Excellence for Parkinson’s Disease Research awarded to the Mayo Clinic Florida; Z.K.W. and R.J.U.); NINDS: AG025327 (J.Z.); NIA: AG033398 (J.Z.); Department of Veterans Affairs (J.B.L.; VA Merit Award 1I01BX000531 to C.P.Z.); Canadian Institutes of Health Research (A.J.S.); Michael Smith Foundation for Health Research (A.J.S.); Pacific Alzheimer Research Foundation (A.J.S.); Canada Research Chairs program (A.J.S.); TRIUMF (A.J.S.); Mayo Clinic Florida Research Committee CR program (Z.K.W. and R.J.U.); the gift from Carl Edward Bolch, Jr. and Susan Bass Bolch (Z.K.W. and R.J.U.); and Grants-in-Aid from the Research Committee of CNS Degenerative Diseases, the Ministry of Health, Labour and Welfare of Japan (K.H. and T.Y.).
Footnotes
Disclosure Statement: The authors report no actual or potential conflict of interest.
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5. Critical references list
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