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Science. Author manuscript; available in PMC Feb 14, 2012.
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PMCID: PMC3279122
NIHMSID: NIHMS349106
Mutations in the RNA Granule Component TDRD7 Cause Cataract and Glaucoma
Salil A. Lachke,1* Fowzan S. Alkuraya,1,2,3,4* Stephen C. Kneeland,5* Takbum Ohn,6 Anton Aboukhalil,1,7 Gareth R. Howell,5 Irfan Saadi,1 Resy Cavallesco,1 Yingzi Yue,1 Anne C-H. Tsai,8 K. Saidas Nair,5 Mihai I. Cosma,5,9 Richard S. Smith,5 Emily Hodges,10 Suad M. AlFadhli,11 Amal Al-Hajeri,11 Hanan E. Shamseldin,2 AbdulMutalib Behbehani,12 Gregory J. Hannon,10 Martha L. Bulyk,1,13,14 Arlene V. Drack,15 Paul J. Anderson,6 Simon W. M. John,5,16§ and Richard L. Maas1§
1Division of Genetics, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA 02115, USA
2Department of Genetics, King Faisal Specialist Hospital and Research Center, Riyadh, 11211, KSA
3Department of Anatomy and Cell Biology, College of Medicine, Alfaisal University, Riyadh 11533, KSA
4Department of Pediatrics, King Khalid University Hospital and College of Medicine, King Saud University, Riyadh 11461, KSA
5Howard Hughes Medical Institute and The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA
6Division of Rheumatology, Immunology and Allergy, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA 02115, USA
7Department of Aeronautics and Astronautics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
8Department of Pediatrics, University of Colorado-Denver, Aurora, CO 80045, USA
9Department of Medicine, Maine Medical Center, Portland, Maine, ME 04102, USA
10Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, Cold Spring Harbor, NY 11724, USA
11Medical Laboratory Medicine, Faculty of Allied Health Sciences, Kuwait University, Kuwait City 13060, KW
12Department of Surgery, Faculty of Medicine, Kuwait University, Alsafat 13110, KW
13Department of Pathology, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA 02115, USA
14Harvard-Massachusetts Institute of Technology Division of Health Sciences and Technology (HST), Harvard Medical School, Boston, MA 02115, USA
15Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, IA 52242, USA
16Department of Ophthalmology, Tufts University School of Medicine, Boston, MA 02115 USA
§ To whom correspondence should be addressed. maas/at/genetics.med.harvard.edu (R.L.M.); simon.john/at/jax.org (S.W.M.J.)
*These authors contributed equally to this work.
Present address: Division of Natural Sciences, Chosun University, Gwangju 501-759, South Korea.
Present address: Center for Regenerative and Developmental Biology, The Forsyth Institute, Cambridge, MA 02142, USA.
The precise transcriptional regulation of gene expression is essential for vertebrate development, but the role of posttranscriptional regulatory mechanisms is less clear. Cytoplasmic RNA granules (RGs) function in the posttranscriptional control of gene expression, but the extent of RG involvement in organogenesis is unknown. We describe two human cases of pediatric cataract with loss-of-function mutations in TDRD7 and demonstrate that Tdrd7 nullizygosity in mouse causes cataracts, as well as glaucoma and an arrest in spermatogenesis. TDRD7 is a Tudor domain RNA binding protein that is expressed in lens fiber cells in distinct TDRD7-RGs that interact with STAU1-ribonucleoproteins (RNPs). TDRD7 coimmunoprecipitates with specific lens messenger RNAs (mRNAs) and is required for the posttranscriptional control of mRNAs that are critical to normal lens development and to RG function. These findings demonstrate a role for RGs in vertebrate organogenesis.
In eukaryotic cells, cytoplasmic RNA granules (RGs) function in determining whether mRNAs undergo degradation, stabilization, or intracellular localization (13). Lower eukaryotes such as yeast harbor RGs that are classified as either processing bodies (PBs) or stress granules (SGs) (3, 4), whereas metazoan cells harbor additional classes of RGs (57). Somatic cells contain both PBs and transport ribonucleoprotein (RNP) particles and accumulate SGs in response to environmental stress (8, 9). PBs contain components of mRNA decay processes like Xrn1-mediated 5′ to 3′ degradation, nonsense-mediated decay (NMD), and microRNA-mediated silencing, and serve as sites where mRNAs can be either stored or degraded (10). In neuronal cells and fibroblasts, RNPs function in the transport and localized translation of mRNAs involved in synapse formation or motility (8, 11). SGs store bulk mRNA during conditions of stress and can interact with PBs to exchange mRNAs that are then directed to translational reinitiation or degradation (9, 12). Lastly, germ cells contain germ cell–specific granules (GCG) that have been implicated in germ cell specification (2, 7).
Proteins with roles in development have been associated with RGs (13), but the importance of this association and that of somatic cell RGs in metazoan organogenesis remains unknown. For example, the precise spatial and temporal expression of key transcription factors is essential for normal transcriptional regulation during development. However, whether a similar level of developmental control exists over the expression of factors involved in posttranscriptional mRNA regulation, and whether this control is required for organogenesis, is unclear. We report the identification of Tudor domain–containing 7 protein, or TDRD7, as an RG component with a highly enriched and conserved pattern of developmental expression in the vertebrate ocular lens. In lens, we identify TDRD7 as a component of a unique class of RNPs and show that these TDRD7-RNA granules (TDRD7-RGs) differentially associate with lens PBs and RNPs that contain a known RG component, STAU1 (STAU1-RNPs). Furthermore, human TDRD7 mutations result in cataract formation via the misregulation of specific, developmentally critical lens transcripts, and Tdrd7 null mutant mice develop cataract as well as glaucoma, the latter defined by elevated intraocular pressure (IOP) and optic nerve damage.
The study of patients with balanced chromosomal rearrangements represents an important entry point to understanding disease mechanisms, and numerous examples exist of disease genes that have been identified by virtue of being disrupted by rearrangement breakpoints (14). As part of the Developmental Genome Anatomy Project (DGAP) (www.bwhpathology.org/dgap), we ascertained a male patient, designated DGAP186, with juvenile cataract and hypospadias who has a de novo balanced paracentric inversion of chromosome 9, 46,XY,inv(9)(q22.33q34.11) (Fig. 1, A and B).
Fig. 1
Fig. 1
TDRD7 mutations in human pediatric cataract. (A) Cataract in DGAP186 (left eye, white arrowhead). (B) Ideogram of normal and inverted chromosome 9 [inv(9)]. Inversion breakpoints are shown by red lines, with a schematic below of TDRD7. Dotted black line (more ...)
We determined that the 9q34.11 breakpoint disrupts the gene NR5A1 (fig. S1). NR5A1 encodes a steroid nuclear receptor protein implicated in hypospadias in mouse and human mutants without cataract (1517). NR5A1 disruption is therefore likely to explain the reproductive tract phenotype and unlikely to account for the cataract phenotype in DGAP186. Analysis of the 9q22.33 breakpoint revealed that TDRD7 is disrupted (Fig. 1, B and C, and fig. S1), and TDRD7 haploinsufficiency as a direct result of the allelic disruption was observed in DGAP186 lymphoblastoid cells at both the RNA and protein levels, (Fig. 1D and fig. S1).
To independently confirm the involvement of TDRD7 in pediatric cataract, we identified a family, F3R, with autosomal recessive congenital cataract. Homozygosity mapping (18) identified a single block of shared homozygosity between the four affected siblings that spanned the TDRD7 locus (Fig. 1E and fig. S2). Furthermore, bidirectional sequencing of TDRD7 uncovered a novel in-frame 3–base pair deletion that removes a highly conserved amino acid, V618 (Fig. 1F and fig. S2). This V618del variant, which was not detected in 320 ethnically matched controls (640 chromosomes), is predicted to disrupt the structure of TDRD7 and is therefore likely to represent a loss-of-function mutation (fig. S2).
To assay the endogenous expression of TDRD7, we turned to mouse and chick embryos. In situ hybridization revealed strong and highly specific expression of Tdrd7 transcripts in the developing mouse lens (Fig. 2A and fig. S3). At embryonic day E12.5, Tdrd7 is expressed in differentiating fiber cells in the posterior lens, whereas the anterior epithelium of the lens (AEL) lacks detectable expression. Expression of chick TDRD7 is also high in the developing lens (Fig. 2B). To confirm a direct causal link between TDRD7 haplo-insufficiency and cataract formation, we used a replication-competent avian sarcoma (RCAS) viral vector (19) to deliver short hairpins that specifically targeted chick TDRD7 to achieve RNA interference–mediated gene knockdown. TDRD7-knockdown short hairpin–mediated RNA interference (shRNA) retroviruses were injected in E2 [Hamburger and Hamilton stage 11 (HH st. 11)] optic vesicles, and the embryos were analyzed for the presence of cataract at E16 (HH st. 42). Injections of green fluorescent protein–expressing control RCAS virus led to highly efficient uptake by lens cells (fig. S3). At E16, a cataract phenotype was observed at significant frequency (17/115, 15%) in chick embryos injected with TDRD7-shRNA RCAS virus but not in those that received control virus (1/48, 2%; P < 0.05) (Fig. 2C and fig. S3). Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis indicated that cataract formation was observed in lenses in which TDRD7 transcripts were reduced to approximately 40% or less of control levels (fig. S3).
Fig. 2
Fig. 2
Tdrd7 deficiency causes cataract and glaucoma. (A) In situ hybridization of E12.5 mouse embryo (coronal section) demonstrates Tdrd7 RNA expression in differentiating lens fiber cells (FC) (arrowhead), and its absence in the AEL (arrow). (B) In situ of (more ...)
To gain further insight into TDRD7 function, we analyzed mutant mice with an N-ethyl-N-nitrosourea (ENU)–induced recessive mutation in Tdrd7. These mice, identified during a screen for glaucoma phenotypes, develop cataracts and high IOP. A nonsense mutation c.2187C>T (Q723X) produces a Tdrd7 null allele, as determined by the absence of TDRD7 protein in homozygous mutants (fig. S4). Within 4 weeks of birth, all Tdrd7 homozygous mutants developed a posterior cataract that became severe with age (Fig. 2, D and E). At later stages, the lens fiber cell compartment developed vacuoles with lens capsule rupture and extrusion of fiber cell mass into the vitreous (Fig. 2, F and G). This feature of the mouse lens phenotype precisely recapitulates the unusual posterior lenticonus (a conical projection of the lens surface) and posterior capsule defects observed in the DGAP186 proband (15). In addition, in some Tdrd7 mutant mice, the mass of fiber cells passed through the pupil into the anterior chamber of the eye (fig. S4). By 4 months of age, iris flattening was detected and anterior chamber depth increased (fig. S5). By 6 months of age, the IOP was elevated in some Tdrd7 mutants, and the incidence of elevated IOP increased with age (Fig. 2H and fig. S5). The ocular drainage structures Schlemm’s canal and the trabecular meshwork normally influence IOP and are located in the angle of the anterior chamber—the angle formed between the iris and the cornea, where the aqueous humor flows out of the anterior chamber. If the egress of aqueous humor is impeded, the accumulated fluid leads to increased IOP, which in turn contributes to retinal ganglion cell (RGC) death and optic nerve atrophy, all of which are hallmarks of glaucoma. In Tdrd7 mutants, the angles are largely normal in morphology with an open-angle configuration, defined by absence of morphologic obstruction (Fig. 2, I and J); the open-angle configuration also predominates in human glaucoma. In Tdrd7 mutant mice, severe optic nerve atrophy characterized by RGC axon loss and excavative remodeling of the optic nerve were observed (Fig. 2, K to N, and fig. S5). Notably, in family F3R, two of the four affected individuals developed glaucoma with open angles and increased IOP after cataract extraction, pointing to the Tdrd7 mutant mouse as a potential model for certain aspects of human glaucoma.
TDRD7 contains five conserved Tudor class domains and three OST-HTH (Oskar-TDRD7-Helix-Turn-Helix)/LOTUS domains (Fig. 1B), which bind methylated arginine residues and RNA, respectively (20). TDRD1, TDRD6, and TDRD7 have been associated with a GCG known as the chromatoid body (CB) that is found in mammalian male germ cells (21, 22). To gain insight into TDRD7’s cellular function, we generated a mouse TDRD7 antibody and analyzed protein expression during mouse embryogenesis. TDRD7 expression between E11.0 and E12.5 becomes markedly enriched in lens fiber cells, where it is expressed in numerous cytoplasmic granules of 0.3 to 0.8 μm diameter (Fig. 3, A and B, and fig. S6). A similar pattern was observed with a second independent TDRD7 antibody (fig. S6). TDRD7 granules were also found in differentiating secondary fiber cells in postnatal day 1 (P1) lens (fig. S6). To determine whether these TDRD7 positive granules contained RNA, we stained mouse E12.5 lens sections with SYTO RNASelect or Pyronin Y, two RNA-specific stains (Fig. 3C and fig. S7). Co-staining with TDRD7 antibody revealed that TDRD7 granules colocalize with RNA in lens fiber cell cytoplasm, and thus constitute bona fide RGs, denoted TDRD7-RGs (Fig. 3, C to E).
Fig. 3
Fig. 3
TDRD7 RNA granules interact with STAU1-RNPs in lens fiber cells. (A) Immunofluorescence (IF) of mouse E12.5 lens (coronal section) with TDRD7 antibody demonstrates highly specific punctate expression in lens FCs and absence in AEL. (B) Higher magnification (more ...)
To establish whether TDRD7 is associated with a specific class of RGs in mouse embryonic lens, we examined the expression of protein markers for different classes of somatic cell RGs (23). Immunostaining of mouse embryonic lens sections with antibodies against the PB markers DCP1A and Ge-1 (23) demonstrated the presence of numerous cytoplasmic PBs in lens fiber cells (Fig. 3F and fig. S8). These structures were also found to contain DDX6/RCK and were dissociated by cycloheximide (CHX) (fig. S9), indicating that they are functional PBs. Costaining these sections with TDRD7 antibody demonstrated that TDRD7-RGs interact with PBs (Fig. 3F and fig. S8). In addition to colocalization, PBs and TDRD7-RGs were occasionally juxtaposed to each other, in an arrangement similar to the docking-type interactions observed for DCP1A and STAU1-RNPs in neurons (fig. S8) (24). Quantification of both colocalizing and docking configurations between TDRD7 granules and PBs indicated a modest but significant degree of overall interaction [12 ± 4%, mean ± SEM, n = 250 PBs; P < 1.5 × 10−5, compared to a model of random distribution throughout the lens (15)].
STAU1 and STAU2, mammalian homologs of the Drosophila RNA-binding protein Staufen, are components of transport RNPs (2527). Staining with STAU1 antibody revealed the presence of numerous STAU1-positive RNPs in lens fiber cells (Fig. 3G). These colocalized to a high degree (29 ± 5%, mean ± SEM, n = 258 granules) with TDRD7 (Fig. 3G and fig. S10). Expression of TDRD7 and STAU1 proteins could be detected as early as E10.5 at the lens vesicle stage (fig. S10). When tested at E11.5, the extent of TDRD7-STAU1 colocalization was highest along the anterior edge of the elongating fiber cell compartment (Fig. 3H), which apposes the AEL at later developmental stages. Similar results were obtained with the second TDRD7 antibody (fig. S10). We also tested for other known components of RGs, namely TIA-1, TIAL1 (TIAR), STAU2, and HuR (ELAVL1) (23), and found that these were not components of mouse lens RGs at E12.5 (fig. S11).
Previous studies have indicated that RGs dynamically interact with each other, and they may share components or participate in mRNA exchange (12). STAU1 is especially relevant, as it is a component of all four RG classes and functions in diverse aspects of RNA metabolism, including mRNA transport and mRNA decay (24, 28, 29). We therefore hypothesized that TDRD7 granules, either alone or through their interaction with STAU1-RNPs and PBs, might regulate the number of RGs in the cell as well as the expression levels of specific lens transcripts (fig. S12). We further postulated that the misregulation of specific mRNAs due to reduced TDRD7 dosage might cause cataract formation. To test these hypotheses, we first performed TDRD7 knockdowns in a U2OS cell line–based assay previously developed to identify genes critical for RG formation (13). These experiments revealed that Tdrd7 knockdown led to significant reductions in the numbers of SGs and PBs (fig. S13). To determine whether this effect was conserved in lens cell lines with higher numbers of SGs and PBs, we examined the human lens cell line SRA01/04 and quantified the results. In these cells, TDRD7-knockdown produced a dramatic reduction in SGs, indicating that TDRD7 is critical for an appropriate response of these cells to stressful stimuli (Fig. 4A and fig. S14). In contrast, TDRD7 knockdown produced only minimal effects on PB numbers. Statistically significant reductions in SG numbers were also observed in Tdrd7-knockdown experiments in a mouse lens–derived epithelial cell line, 21EM15 (henceforth Tdrd7-KD 21EM15) (30), where the reduction in Tdrd7 expression of 60 ± 4% (mean ± SEM, n = 3) approximates that in DGAP186 lymphoblastoid cells due to haploinsufficiency (figs. S13 and S14).
Fig. 4
Fig. 4
Tdrd7 is critical for RNA granule formation and HSPB1 expression. (A) SRA01/04 cell line shows increased numbers of P-bodies (detected by DDX6/RCK antibody) and stress granules (detected by elF3b antibody) in response to oxidative stress (sodium arsenite, (more ...)
Next, to identify Tdrd7 deficiency–induced changes in gene expression, we performed micro-array analyses in biological triplicate on Tdrd7-KD 21EM15 cells and compared the in vitro results to those obtained for Tdrd7 null lenses. In initial microarray experiments, we observed that 21EM15 cells expressed 56 of the top 100 genes that are expressed during embryonic lens development, including Tdrd7. Thus, 21EM15 cells at least partly recapitulate the molecular events during endogenous lens development. In Tdrd7-KD 21EM15 cells, compared to 21EM15 cells infected with control vector, ~6% of expressed genes were differentially regulated at a threshold of a 1.3-fold or greater (table S1). Of particular interest, key genes that encode SG and PB components (G3bp, Hspb1, and Ddx6), mouse homologs of known human cataract genes (Crygs and Epha2), and genes involved in fiber cell differentiation (Prox1) were all significantly down-regulated (Fig. 5A and fig. S15).
Fig. 5
Fig. 5
TDRD7 regulates mRNAs critical to lens development and RG function. (A) Microarray analyses of Tdrd7-KD 21EM15 mouse lens cells reveals down-regulation of RNA granule genes and human cataract genes and up-regulation of other genes normally down-regulated (more ...)
To extend these results in vivo, we further analyzed the Tdrd7 null mouse lens. As expected from Western blot analyses (fig. S4), immunofluorescence confirmed a complete absence of TDRD7 in the lens (Fig. 4B). We then undertook a microarray expression analysis of isolated lenses from Tdrd7 null mutants and littermate controls, focusing on postnatal days P4, three weeks before overt cataract appearance, and P30, when cataracts are fully penetrant. To accurately identify Tdrd7-dependent genes that function in lens development, we compared the P4 and P30 Tdrd7 mutant mouse lens microarray data with that obtained from the Tdrd7-KD 21EM15 mouse lens cell line. Comparison of differentially regulated genes (DRGs) from the P4 and P30 Tdrd7 null lens microarray data sets (each versus control), and the DRGs in Tdrd7-KD 21EM15 cells (versus control) identified several biologically relevant genes that were concordantly regulated in all three data sets (Fig. 5, A and B). These DRGs were classified into five distinct functional categories, denoted Classes I to V: Class I, genes involved in SG assembly or function; Class II, genes involved in P-body function and/or encoding helicases; Class III, genes linked to cataract or other ocular phenotypes; Class IV, crystallin genes (apart from Class III); and Class V, genes normally down-regulated during lens fiber cell differentiation (Fig. 5A). Whereas Tdrd7 null lenses and Tdrd7-KD 21EM15 lens cells exhibit overlapping expression, each also exhibits unique categories of gene expression (e.g., Classes IV and V, respectively), and thus constitute complementary systems for revealing the full spectrum of DRGs attributable to Tdrd7 loss-of-function.
Of 12 DRGs whose expression was significantly altered in Tdrd7-KD 21EM15 lens cells and that were relevant to lens development or RG function, six (Hspb1, Ddx6, Ddx26, Epha2, Prox1, and Crygs) were also concordantly down-regulated in Tdrd7 null lens data sets. Two other genes that were down-regulated in both P4 and P30 Tdrd7 null lenses, Sparc and Crybb3, are associated with cataracts and are thus also of interest (31, 32) (Fig. 5B). Thus, from comparative analyses of the microarray data, we identified eight genes that were significantly down-regulated in the Tdrd7 null lens, Tdrd7-KD 21EM15 lens cells, or both, and that have biologically plausible links to lens development or RG function.
To determine whether the altered expression of these eight genes resulted from the direct or indirect action of TDRD7, we used qRT-PCR to confirm the microarray expression changes in P30 Tdrd7 null lens RNA. This analysis confirmed the down-regulation of Crybb3, Hspb1, Sparc, and Epha2 and of several other genes (fig. S15). We then performed RNA immunoprecipitation (RIP) experiments with TDRD7 antibody, followed by RNA isolation and RT-PCR. Because 21EM15 cells substantially recapitulate the expression profile of the developing lens, and there is considerable concordance in DRGs between the corresponding Tdrd7 loss-of-function state, we employed 21EM15 lens cells for these experiments. Crybb3 and Hspb1 transcripts were markedly enriched in the TDRD7 immunoprecipitations, whereas Epha2 transcripts were only modestly enriched and Sparc transcripts were not enriched (Fig. 5C). Thus, based on their strong Tdrd7-expression dependence and evidence for direct binding from the RIP experiments, we conclude that Crybb3 and Hspb1 mRNAs are likely to be direct targets of TDRD7 regulatory function in the lens.
An attractive model emerges from these results. During lens development, AEL cells migrate into the lens “equatorial zone,” where they differentiate into fiber cells. During fiber cell differentiation, because of the nuclear degradation process that helps promote lens clarity, most AEL-expressed genes cease transcription. By binding and stabilizing specific transcripts, TDRD7 and other RG components may facilitate the translation of crystallin mRNAs necessary to achieve the high protein levels and tight packing that provides for ocular transparency at high refractive index. TDRD7 also functions by maintaining mRNA expression levels of the heat shock gene Hspb1, which encodes a stress response chaperone protein that is a stress granule component that functions in mRNA decay (33, 34). Down-regulation of Hspb1 mRNA levels is one of the earliest and quantitatively most striking gene expression changes we detect in Tdrd7 null lenses. HSPB1 down-regulation at the protein level was detected in P4 and P22 lenses (Fig. 4B and fig. S15). Interestingly, HSPB1 also interacts with several lens crystallin proteins (35) and stabilizes αB-crystallin (36). Moreover, destabilizing mutations in αB-crystallin are a known cause of congenital cataract (OMIM ID 123590). Thus, Crybb3 and Hspb1 are excellent candidates to contribute to cataract formation in Tdrd7 loss-of-function mutants.
TDRD7 may also regulate certain transcripts indirectly. For example, TDRD7 deficiency results in reduction of Sparc transcripts, and Sparc null mice develop late-onset cataracts that resemble Tdrd7 null mutant cataracts (31). These results place Tdrd7 function upstream of Sparc mRNA expression and, because Tdrd7 is involved in the regulation of multiple cataract genes (fig. S15), can explain the earlier cataract onset in Tdrd7 mutants compared with Sparc mutants. In addition, Sparc and Epha2 mRNAs have been described as direct targets of STAU1-RNPs, and Epha2 mutations cause cataracts in both mouse and human (26, 37). Because STAU1-RNPs and TDRD7-RGs interact in the lens, Sparc and Epha2 down-regulation in Tdrd7 mutant lenses may reflect the disruption of this interaction between distinct RNPs and provide an additional mechanism of action for TDRD7 in the lens. A model encompassing both direct and indirect mechanisms of TDRD7 action in the developing lens is depicted in Fig. 5D.
In sum, TDRD7 is an RNA granule component that is highly enriched in the developing lens; TDRD7 perturbation in chick, mouse, and human causes cataract. In the lens, TDRD7-RGs play an essential role in the regulation of specific genes that are critical for lens development, including those responsive to stress, such as Hspb1. Tdrd7 deficiency disturbs this regulatory mechanism and leads to cataract. The RNA granule function of TDRD7 may also be deployed in other tissues and cell types at various developmental stages. For example, TDRD7 is a component of a testis-specific RNA granule, the chromatoid body (CB) (21), and we observe that Tdrd7 null mice exhibit male sterility due to an arrest in spermatogenesis at the round spermatid stage, likely due to a CB defect (fig. S16). Lastly, Tdrd7 null mice develop elevated IOP and other features of glaucoma as they age. IOP elevation and glaucoma can result from oxidative and other stresses that damage the aqueous humor drainage tissues (38). It is possible that the IOP elevation in Tdrd7 null mice and in the two human patients who developed glaucoma results, at least in part, from abnormalities in the protective stress response or in other TDRD7-related functions in the drainage tissues. Thus, the data demonstrate that human organogenesis defects can result from perturbation of a distinct, tissue-specific RG component that posttranscriptionally regulates the levels of developmentally critical mRNAs.
Supplementary Material
Materials and Methods, Figs. S1 to S16, Table S1, References
Acknowledgments
This work was supported by R01 EY10123, R01 HD060050, P01 GM061354, KACST 08-MED497-20, R01 EY11721, The Barbara and Joseph Cohen Foundation, The Peace by Pieces Fund, and the Dubai Harvard Foundation for Medical Research. S.M.A. is supported by a grant from Kuwait University (YM01/09). A.V.D. was supported by a Marjorie Carr Adams Career Development Award from the Foundation Fighting Blindness. A.A. is supported by an American Heart Association Predoctoral Fellowship. S.W.M.J. and G.J.H. are Investigators of the Howard Hughes Medical Institute. We thank C. Cepko for advice on the chick experiments, L. Reinholdt for assistance in assessing male sterility, A. Bell for technical assistance, J. Reddan and V. Reddy for their gifts of lens cell lines, and the patients and their families for participating in this research. All microarray data are deposited in the Gene Expression Omnibus database (www.ncbi.nih.gov/geo), and the accession number for the SuperSeries for all data files is GSE25812.
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