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Adhesion phenotype analysis of the Σ1278b (labeled Sigma) and S288c transformants used in the transcriptome analysis. Wild-type, MSS11 deletion (mss11), and overexpression (MSS11) strains were analyzed as indicated. (A) “Mat” formation on 0.3% YPD agar after 7 days of growth. (B) Measurement of “Mat” growth at day 5, 7, and 12, respectively. (C) Invasive growth of transformants. Transformants were grown in selective media and spotted on YPD plates: Total growth after 6 days incubation (left) and invaded cells revealed following subsequent plate washing (right). Transformants grown to stationary phase in liquid minimal media (SCD) assayed for (D) the degree of culture hydrophobicity and (E) their ability to flocculate.