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Clin Infect Dis. Mar 1, 2012; 54(5): 694–700.
Published online Dec 9, 2011. doi:  10.1093/cid/cir896
PMCID: PMC3275758
Corticosteroid Therapy for Liver Abscess in Chronic Granulomatous Disease
Jennifer W. Leiding,1 Alexandra F. Freeman,1 Beatriz E. Marciano,1 Victoria L. Anderson,1 Gulbu Uzel,1 Harry L. Malech,2 SukSee DeRavin,2 David Wilks,3 Aradhana M. Venkatesan,4 Christa S. Zerbe,1 Theo Heller,5 and Steven M. Hollandcorresponding author1
1Laboratories of Clinical Infectious Diseases
2Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland
3Western General Hospital, Edinburgh, United Kingdom
4Radiology and Imaging Sciences, Clinical Center
5Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland
corresponding authorCorresponding author.
Correspondence: Steven M. Holland, MD, CRC B3-4141 MSC 1684, Bethesda, MD 20892-1684 (smh/at/nih.gov).
Received June 5, 2011; Accepted October 31, 2011.
Abstract
Liver abscesses in chronic granulomatous disease (CGD) are typically difficult to treat and often require surgery. We describe 9 X-linked CGD patients with staphylococcal liver abscesses refractory to conventional therapy successfully treated with corticosteroids and antibiotics. Corticosteroids may have a role in treatment of Staphylococcus aureus liver abscesses in CGD.
Chronic granulomatous disease (CGD) is a primary phagocyte defect complicated by a limited spectrum of bacterial and fungal infections as well as dysregulated inflammation. The latter often leads to granuloma formation affecting the gastrointestinal and genitourethral tracts. Five distinct genetic forms affect the components of nicotinamide adenine dinucleotide phosphate oxidase: gp91phox, p22phox, p47phox, p67phox, and p40phox, resulting in diminished phagocyte respiratory burst [1, 2]. The lungs, skin, lymph nodes, and liver are the most frequent sites of infection, with liver abscesses occurring in approximately 35% of patients. These abscesses are notoriously difficult to treat without surgery [3].
Corticosteroids effectively manage the obstructive and inflammatory disorders in CGD and are well tolerated with minimal infectious complications at low doses [46]. In addition, multiple case reports cite success in concomitant use of corticosteroids with antimicrobials for pneumonia [710] and in the syndrome of “mulch pneumonitis,” the acute inflammatory and necrotizing granulomatous lung disease following inhalation of organic matter such as mulch or hay [11]. In mulch pneumonitis, steroids are thought to decrease the acute severe inflammatory response evoked by fungal cell walls, allowing better ventilation and antifungal penetration into granulomatous lesions.
Over the last several years, the important role of liver dysfunction in CGD has been recognized [12, 13]. Contributing factors include nodular regenerative hyperplasia, portal hypertension, and liver abscesses. Yamazaki-Nakashimada et al first described the use of steroids in the treatment of refractory liver abscesses in 2 CGD patients [10]. While treating CGD liver abscesses refractory to or intolerant of standard surgery and parenteral antibiotics, we used steroids to ameliorate the aberrant inflammatory response. We report the dramatic effects steroid coadministration had on outcome of liver abscesses in CGD.
Patient 2
This 20-year-old Filipino man had X-linked CGD diagnosed in infancy. He had multiple pneumonias, including Aspergillus, and lymphadenitis. A liver abscess was resected at age 8 years. At age 19 years, he presented with fever, cough, and right upper quadrant pain. Computed tomography (CT) showed right lower lobe consolidation and a large heterogeneous fluid collection in the right lobe of the liver. Liver function tests showed alkaline phosphatase 322 U/L, aspartate aminotransferase 46 U/L, alanine aminotransferase 62 U/L, total bilirubin 1.1 mg/dL, and albumin 2.7 g/dL; percutaneous culture grew methicillin-sensitive Staphylococcus aureus (MSSA). Imipenem, fluconazole, and rifampin for 2 months were associated with progression of his liver disease. On transfer to the National Institutes of Health, he had abdominal pain, fever, and elevated inflammatory markers (erythrocyte sedimentation rate [ESR] 92 mm/h and C-reactive protein [CRP] 132 mg/L). A new lesion in the left hepatic lobe caused intrahepatic congestion and compression of the right hepatic vein, precluding resection (Figures 1A and 1B). In view of the patient’s extensive multiloculated abscesses, their location, his previous liver surgery, and the inability to safely preserve the right hepatic vein during surgery, we initiated vancomycin, levofloxacin, and methyprednisolone 1.5 mg/kg per day; rifampin was stopped. After 1 week, inflammatory markers had dramatically reduced (ESR 23 mm/h, CRP 4.9 mg/L), liver functions improved, and CT and magnetic resonance imaging showed improvement in liver abscesses. He was transitioned to oxacillin, levofloxacin, and prednisone, which was tapered to 0.2 mg/kg per day over 3 months. At 0.2 mg/kg per day, CT showed 2 new low-density lesions, but his clinical status was stable. Prednisone was increased to 0.4 mg/kg per day, and the patient was transitioned to oral cefadroxil. Over the next 3 months, his liver lesions completely resolved despite his inadvertent discontinuation of steroids and antibacterials (Figures 1C and 1D). Eleven months after initial presentation, he developed an unrelated MSSA submental abscess, which was successfully treated.
Figure 1.
Figure 1.
Computed tomography (CT) and magnetic resonance imaging (MRI) on patient 2. Axial (A) and coronal (B) contrast-enhanced CT images demonstrate a multiseptated abscess within the right lobe of liver measuring 8 cm in maximal craniocaudal dimension (white (more ...)
Figure 2.
Figure 2.
Computed tomography (CT) and magnetic resonance imaging (MRI) on patient 9. Axial (A) and coronal (B) contrast-enhanced CT images demonstrate a multiseptated abscess (white arrowheads [A]; white arrow [B]) within the right hepatic lobe measuring 12.4 (more ...)
We describe 9 cases of severe, life-threatening liver abscesses in X-linked CGD that progressed despite appropriate antibacterial therapy and drainage, where appropriate, that responded to moderately high doses of corticosteroids tapered over several months. All patients presented with nonspecific symptoms including fever, malaise, abdominal pain, and elevated inflammatory markers; 6 had prior histories of liver abscess (Table 1). This agrees with previous data that prior liver abscesses predict recurrence [12, 13]. The best results were obtained when the steroids were initiated at a moderately high dose and their administration was prolonged; local hepatic inflammation often flared when steroids were tapered. In those patients in whom extended steroids were used along with appropriate antibiotics, no disease worsened, and 8 of 9 patients achieved clinical cure without surgery.
Table 1.
Table 1.
Demographics and History
Hepatic abscesses are a common complication of CGD, are typically due to S. aureus, and are difficult to treat [14]. Lublin et al reported that 16 of 61 cases required multiple procedures with an overall surgical complication rate of 56% [3]. Despite the low mortality rates of liver abscesses treated with antibiotics and surgery, the morbidity is high and there is a high rate of recurrence. Perhaps more important, hepatic dysfunction with portal venopathy and nodular regenerative hyperplasia (NRH) are highly associated with liver abscesses and are major predictors of mortality in CGD [12, 13], independent of level of superoxide production [1]. The mechanism is presumed to be noncirrhotic portal hypertension caused by microvascular insults from repeated abscesses [12]. It is possible that hepatic regeneration after surgery causes venopathy and NRH. However, it remains unclear whether liver abscesses are causes, effects, or simply parallel markers of this predisposition to severe liver dysfunction.
Dysregulated inflammation in CGD is probably important in the late complications of the disease. Morgenstern et al demonstrated that both early and delayed inflammation was exaggerated in X-CGD mice independent of infection [15]. Proposed mechanisms of hyperinflammation include defective neutrophil apoptosis [16]; skewed nuclear factor-κB signaling [17]; upregulation of tumor necrosis factor α, interleukin (IL) 17, IL-6, and granulocyte colony-stimulating factor [18]; impaired degradation of leukotriene B4 and C5a [19]; prolonged IL-8 messenger RNA activation [20]; impaired activation of Nrf2 [21]; defective tryptophan catabolism in mice [22] but not humans with CGD [23]; and deficient peroxisome proliferator-activated receptor γ activation [24]. Regardless of the specific mechanisms of hyperinflammation, corticosteroids reduce the activation, proliferation, and differentiation of many cells involved in inflammation, including macrophages and lymphocytes, the key components in granuloma formation [25].
There are precedents for the successful use of steroids in CGD during acute infection when coadministered with appropriate antimicrobials. Mulch pneumonitis appeared to benefit from steroid therapy when administered along with adequate antifungals [11]. The clinical and radiographic pattern of CGD mulch pneumonitis patients is similar to that seen in hypersensitivity pneumonitis, in which exuberant host response to environmental pathogens is typically treated by steroids [26]. Similar results have been reported in patients with invasive pulmonary Nocardia [27], chronic cystitis [28], and in 2 cases of liver abscesses [10]. The overlap between this aspect of CGD and hypersensitivity reactions is provocative.
The patients in this report had either inoperable disease, were too ill to undergo hepatic resection, or refused surgery. Corticosteroids as adjuncts to antibiotics were able to overcome weeks to months of static or progressive liver abscesses in patients with CGD. Corticosteroids were started at a dose of approximately 1 mg/kg daily and continued for an average of 2–3 weeks before being tapered over several months (Table 2). Within days of initiating corticosteroid therapy, patients exhibited clinical improvement, substantial decrease in lesions on imaging, and decrease in inflammatory markers. There was a fast decline in CRP and ESR after initiation of corticosteroids that continued to slowly decline over the ensuing months. All patients were continued on organism-specific antimicrobials, and only 1 of 9 eventually required surgery. Interestingly, the patient who required surgery (patient 3) had received the least and shortest course of steroids. Although there was no uniform steroid taper, the average duration was 3 months. Faster taper was associated with symptom, sign, and radiographic exacerbation, resulting in addition of antimicrobials and increased corticosteroids. Complications of steroid therapy were transient sleep disturbances, typical hyperphagia, and mild weight gain, all of which were reversible.
Table 2.
Table 2.
Management and Outcome
As is often true in retrospective case series of patients managed by independent groups, the treatments, doses, and durations of therapy received by the patients varied. However, by pulling together 9 cases of liver abscess in CGD with somewhat different outcomes, we can infer some general features, recognizing that prospective trials of surgery versus steroid therapy are unlikely to be performed. The duration of steroid therapy appears to be important, with radiographic evidence of exacerbation as doses became very low. In our series, the only patient who required open surgical excision after treatment with corticosteroids was not the one with the largest abscess, but the one who received the least steroids. In contrast, long-term high-dose steroids have very real consequences, including other opportunistic infections and metabolic effects. The necessity for intravenous antibiotic therapy is also unclear, but the coadministration of antibiotics with steroids is sensible and probably required.
Liver abscesses in CGD have not been eliminated by antibiotic prophylaxis, are generally survivable, and often recur. However, adult-onset liver dysfunction is highly associated with adverse outcomes in CGD [12, 13]. Interestingly, the mortality curves between high-risk and low-risk forms of CGD do not diverge until adulthood, implying that accumulated toxicities of the disease may play a role in outcome [1]. We cannot know at this point whether the long-term consequences of steroid management of CGD liver abscesses are preferable to surgical management. However, it is clear that in the short term, at least in some cases, steroids coadministered with antibiotics allow cure of CGD liver abscesses without surgery.
Notes
Financial support.
This work was supported by the Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health.
Potential conflicts of interest.
All authors: No reported conflicts.
All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed.
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