PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of arthresbiomed central web sitesearch.manuscript submission.see also journal with issn 1478-6354registration.reference to the article.journal front page.
 
Arthritis Res. 2001; 3(Suppl A): P048.
Published online Jan 26, 2001. doi:  10.1186/ar217
PMCID: PMC3273181
In vitro autoreactivity against autologous keratinocytes in patients with rheumatoid and juvenile idiopathic arthritis
K Štechová,1 P Vavrincová,1 H Reitzová,1 and I Hromadníková1
12nd Dep. of Paediatrics, University Hospital Motol, Prague, Czech Republic
Supplement
21st European Workshop for Rheumatology Research
Conference
21st European Workshop for Rheumatology Research
1-4 March 2001
Vienna, Austria
Received January 15, 2001
 
In patients with rheumatoid arthritis or juvenile idiopathic arthritis we observed the tendency of peripheral blood mononuclear cells to induce graft versus host disease like histhopathological changes of grade II or above (evaluated according to standard Lerner's classification) when co-cultured in vitro with autologous skin explants. The aim of this study was to verify if observed skin damage was really of an autoimmune origin and we also tried to compare results with autoreactivity directed against autologous synovium. We suppose that humoral as well as cellular autodestructive mechanisms may be involved in the pathogenesis of observed skin damage.
Methods
To prove this hypothesis (cellular autodestruction)we used 51Cr release cytotoxic test where peripheral blood mononuclear cells were co-cultured with autologous synovial cells as well as with autologous keratinocytes.
Results
We found that patient's peripheral blood mononuclear cells lysed both autologous keratinocytes (specific lysis 60%) as well as autologous synovial cells (59% specific lysis). No specific lysis of autologous keratinocytes and synovial cells was observed in healthy controls.
We suppose that peripheral blood mononuclear cells might recognise similar autoantigen(s) expressed on epidermal cells that might give rise an autoimmune response in synovium.
Articles from Arthritis Research are provided here courtesy of
BioMed Central