Search tips
Search criteria 


Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Cell. Author manuscript; available in PMC 2012 February 5.
Published in final edited form as:
PMCID: PMC3272504

Breaking the Diffraction Barrier: Super-Resolution Imaging of Cells


Anyone who has used a light microscope has wished that its resolution could be a little better. Now, after centuries of gradual improvements, fluorescence microscopy has made a quantum leap in its resolving power due, in large part, to advancements over the past several years in a new area of research called super-resolution fluorescence microscopy. In this Primer, we explain the principles of various super-resolution approaches, such as STED, (S)SIM, and STORM/(F)PALM. Then, we describe recent applications of super-resolution microscopy in cells, which demonstrate how these approaches are beginning to provide new insights into cell biology, microbiology, and neurobiology.

For centuries, light microscopy has greatly facilitated our understanding of how cells function. In fact, entire fields of biology have emerged from images acquired under light microscopes. For instance, more than 300 hundred years ago, Antonie van Leeuwenhoek used his self-ground optical lenses to discover bacteria and commence the field of microbiology. Then, ~200 years later, Ramón y Cajal used light microscopes to visualize Golgi-stained brain sections and create beautiful drawings of neurons, which led to his ingenious vision of how information flows in the nervous systems and helped to form modern neurobiology.

Indeed, one major element that makes light microscopy so powerful in biological research is the development of various staining methods that permit the labeling of specific molecules and cells. For example, fluorescence in situ hybridization (FISH) detects DNA and RNA molecules with specific sequences, whereas immunofluorescence labels and fluorescent proteins allow the imaging of particular proteins in cells (Giepmans et al., 2006). Even single molecules within a living cell can be visualized when these labeling strategies are combined with highly sensitive optical schemes and detectors (Lord et al., 2010; Xie et al., 2008).

The unrivaled combination of molecule-specific contrast and live-cell imaging capability makes fluorescence microscopy the most popular imaging modality in cell biology. Browse through any cell biological journal, and the impact of fluorescent microscopy is obvious, with > 80% of the images of cells in the book usually acquired with a fluorescent microscope. However, the application of fluorescence microscopy to many areas of biology is still hindered by its moderate resolution of several hundred nanometers. This resolution is approximately the size of an organelle and thus is inadequate for dissecting the inner architecture of many subcellular structures.

The resolution for optical microscopy is limited by the diffraction, or the “spreading out,” of the light wave when it passes through a small aperture or is focused to a tiny spot. Because this property is intrinsic to all waves, breaking the diffraction barrier of light microscopy has been deemed impossible for a long time. However, such limitations have not deterred a small group of scientists from pursuing “super-resolution” fluorescence microscopy that breaks through this seemingly impenetrable barrier.

The risk has paid off abundantly. Recently, these research teams have developed several optical microscopy techniques that have shattered the diffraction barrier, improving spatial resolution by an order of magnitude or more over the diffraction limit. Most importantly, these techniques are beginning to provide insights into biological processes at the cellular and molecular scale that were hitherto unattainable. In this Primer, we review the technological advances in the burgeoning field of “super-resolution fluorescence microscopy.” Then, we describe the application of these techniques to various areas of biology, which have quickly demonstrated the great promise of this exciting new area of bioimaging.

Beating the Diffraction Limit of Resolution

When light is focused by the objective of a microscope, the notion of light “rays” converging to an infinitely sharp “focal point” does not happen. Instead, the light wave forms a blurry focal spot with a finite size due to diffraction (Figure 1Afig1). The size of the spot depends on the wavelength of the light and the angle at which the light wave converges; the latter is, in turn, determined by the numerical aperture of the objective. The width of the spot at half-maximum intensity (i.e., full width at half maximum) is ~0.6 λ/NA, wherein λ is the wavelength of the light and NA is the numerical aperture of the lens. Similarly, a point emitter, such as a single fluorescent molecule, also appears as a blurry spot with a finite size when imaged through a microscope. The intensity profile of this spot, which defines the point spread function (PSF) of the microscope, has approximately the same width as that of the focal spot described above. Consequently, two identical emitters separated by a distance less than the width of the PSF will appear as a single object, making them unresolvable from each other (Figure 1A).

Figure 1
Diffraction-Limited Resolution of Conventional Light Microscopy

This resolution limit was originally recognized by Ernst Abbe ~150 years ago, and thus, it is also called the Abbe limit (Abbe, 1873). The diffraction-limited image resolution of objective lens with a high numerical aperture is ~250 nm perpendicular to the direction of light propagation (i.e., in the lateral dimensions) and 550 nm parallel to the direction of light propagation (i.e., in the axial dimension) (Figure 1A). Many subcellular structures are smaller than these resolution limits, and therefore, they are unresolvable by light microscopes (Figure 1B).

For many years, several imaging techniques have pushed the boundary of the diffraction limit of light microscopy. Among these methods, confocal microscopy and multiphoton fluorescence microscopy not only enhance the image resolution, but also reduce the out-of-focus fluorescence background, allowing optical sectioning and thus three-dimensional imaging. In addition, infrared light experiences a lower amount of scattering from tissues, allowing deep tissue imaging with two-photon microscopy (Zipfel et al., 2003). 4Pi microscopy and I5M use two opposing objective lenses to increase the effective numerical aperture of the microscope and thereby improve the image resolution (Gustafsson et al., 1995; Hell and Stelzer, 1992; Hell, 2003). Although these methods significantly improve the resolution, they are still fundamentally limited by diffraction and, in practice, achieve resolutions of ~100 nm in all three dimensions (Hell, 2003).

The diffraction-limited resolution occurs only to light that has propagated for a distance substantially larger than its wavelength (i.e., in the far field). Therefore, one route to bypass this constraint is to place the excitation source or detection probe (usually an optical fiber, a metal tip, or simply a small aperture) near the sample (i.e., in the near field) (Synge, 1928). Indeed, near-field microscopy has achieved resolution substantially below 100 nm (Betzig et al., 1986; Lewis et al., 1984; Novotny and Hecht, 2006; Pohl et al., 1984). However, the requirement that the excitation source or detection probe be physically close to the target object (often within tens of nanometers) has made it difficult to look “into” a cell or a piece of tissue with near field microscopy, limiting the applications of this technique in biology.

It was not until recently that several novel fluorescence microscopy approaches completely shattered the diffraction limit of image resolution in the far field. In general, all of these approaches generate “diffraction-unlimited images” by using the physical properties of fluorescent probes to distinguish emissions from two nearby molecules within a diffraction-limited region. These super-resolution approaches can be divided into two primary classes. The first category is ensemble imaging approaches that use patterned illumination to spatially modulate the fluorescence behavior of molecules within a diffraction-limited region, such that not all of them emit simultaneously, thereby achieving subdiffraction limit resolution. This category includes stimulated emission depletion (STED) microscopy (Hell and Wichmann, 1994; Klar and Hell, 1999) and the related RESOLFT technology (Hofmann et al., 2005), as well as saturated structured illumination microscopy (SSIM) (Gustafsson, 2005; Heintzmann et al., 2002). The second category takes advantages of single-molecule imaging, using photoswitching or other mechanisms to stochastically activate individual molecules within the diffraction-limited region at different times. Images with subdiffraction limit resolution are then reconstructed from the measured positions of individual fluorophores. This second class has been termed stochastic optical reconstruction microscopy (STORM) (Rust et al., 2006), photoactivated localization microscopy (PALM) (Betzig et al., 2006), and fluorescence photoactivation localization microscopy (FPALM) (Hess et al., 2006).

Although these two categories of methods use different approaches to accomplish subdiffraction resolution, these techniques also share important commonalities. In both cases, a physical or chemical property of the fluorophore is used to maintain neighboring molecules in different states (i.e., “on” and “off”), enabling them to be resolved from each other (Hell, 2007).

Super-Resolution Fluorescence Microscopy by Patterned Illumination

In this category of techniques, a patterned field of light is applied to the sample to manipulate the fluorescence signal emitting from its fluorophores. This spatial modulation can be implemented either in a “positive” or “negative” manner. In the positive case, the light field used to excite the sample and generate fluorescence is directly patterned. In contrast, the negative patterning approach enlists the help of an additional patterned light field to suppress the population of molecules that can fluoresce in the sample. In both of these approaches, the spatial information encoded into the illumination pattern allows neighboring fluorophores to be distinguished from each other, leading to enhanced spatial resolution.

Negative Patterning: STED and RESOLFT Microscopy

In STED microscopy, the patterned illumination prevents fluorophores from emitting light (Hell, 2007; Hell and Wichmann, 1994; Klar and Hell, 1999). This suppression is achieved by stimulated emission, a process in which a light source, called the depletion light, brings an excited fluorophore down to the lowest energy state (i.e., the ground state) before it can emit fluorescence signal. In practice, the depletion light is applied as a pattern surrounding the focal spot of the excitation laser. This reduces the size of the region of molecules that fluoresce, as if the “focal spot” of the microscope is sharpened (Figure 2Afig2). Scanning this sharpened spot across a sample then allows a super-resolution image to be recorded. Thus, this negative patterning approach elegantly generates a positive image without the need of any postacquisition processing.

Figure 2
Super-Resolution Fluorescence Microscopy by Patterned Illumination

It is important to note that the depletion light pattern itself is created by the same diffraction-limited optics. Therefore, if the fluorophores respond to the depletion light in a linear manner, the resolution enhancement would be rather limited. STED microscopy surpasses this limit by taking advantage of the saturated response of fluorophores: once the depletion laser intensity is above the saturation level, the number of fluorophores remaining in the excited state (and thus capable of generating fluorescence) approaches zero. Thus, when a ring-shaped depletion light with peak intensity significantly above the saturation level is applied to the sample, only the molecules within a small region near the center of the ring can generate fluorescence (Figure 2A). The size of this region, and thus the resolution of the microscope, scales approximately with the inverse square root of the intensity of the depletion light (or δΔ1+IdepIsat, wherein δ is the resolution, Δ is the full width at half maximum of the diffraction-limited focal spot size, and Idep is the intensity of the depletion laser) (Hell, 2007).

In principle, this approach allows unlimited resolution improvement given an infinitely strong depletion light source. In practice, a number of factors influence the resolution of STED, including aberrations in the optics, scattering from the sample, and the photostability of the fluorophores. STED microscopy has reached a remarkable resolution of 6 nm using strong depletion intensity to image fluorescent defects in diamonds, which almost never photobleach (Rittweger et al., 2009). On biological samples, STED imaging has achieved a resolution of 20 nm when using organic dyes and 50–70 nm resolution when using fluorescent proteins (

In addition to stimulated emission, other saturable optical transitions that send the molecule to dark states can also be used to shrink the area of molecules that fluoresce in a focal spot (Bretschneider et al., 2007; Hofmann et al., 2005). This extension of the STED approach, called reversible saturable optically linear fluorescence transitions (RESOLFT) microscopy, allows super resolution to be implemented with a substantially lower-depletion light intensity, causing less damage to delicate biological samples (Hell, 2007; Hofmann et al., 2005).

Positive Patterning: Structured Illumination Microscopy

Structured illumination microscopy (SIM) improves image resolution by using positive patterning of the excitation light (Heintzmann and Gustafsson, 2009), which is typically a sinusoidal pattern created by combining (i.e., interfering) two light beams. As the result, an image snapshot of the sample becomes the product of the sample structure itself and this excitation pattern (Figure 2B). A final image is then computationally reconstructed from multiple snapshots collected by scanning and rotating the pattern. In this process, the additional spatial modulation from the excitation pattern brings enhanced spatial resolution into the reconstructed image (Gustafsson, 2000; Heintzmann and Cremer, 1999).

As is true for the depletion light pattern used in STED, the illumination pattern created by interference is also limited by diffraction. Therefore, when the fluorescence signal scales linearly with the intensity of the excitation light, SIM results only in a doubling of spatial resolution (Figure 2B), which is ~100 nm in the lateral dimensions(Gustafsson, 2000).

Like with the negative patterning approach, the saturating response of the fluorophore can also be exploited here to further enhance the resolution (Gustafsson, 2005; Heintzmann et al., 2002). With sufficiently strong excitation, the fluorescence emission from a fluorophore will saturate. Saturated SIM (SSIM) utilizes this phenomenon to create sharp dark regions where the excitation pattern has zero intensity, providing image resolution significantly beyond the diffraction limit (Figure 2B). With this approach, a resolution of 50 nm has been obtained for imaging fluorescence microspheres (Gustafsson, 2005).

Fluorescent Probes

The basic form of SIM (i.e., the linear form) does not rely on any special photophysics of the fluorophores but purely on the optics of the microscope. Therefore, any fluorescent probe that is compatible with conventional fluorescence imaging is compatible with SIM. In addition, multicolor imaging can be achieved with SIM as it is with conventional fluorescence microscopy. Up to four colors can be imaged in the visible to near infrared (IR) spectrum range without incurring much crosstalk between the different color channels. As an example, Figure 2D shows a three-color SIM image of DNA, lamin B, and nuclear pore complexes in the nucleus (Schermelleh et al., 2008). In contrast, SSIM requires special fluorophores that have high photostability because the fluorophores are maintained in the highly reactive excited state most of the time in this imaging scheme.

STED microscopy can also use a wide range of existing fluorescent probes because all fluorophores can undergo stimulated emission ( In practice, dyes that are photostable under a strong depletion light, such as Atto 647N, Atto 590, and Atto 565, provide greater resolution enhancement. Fluorescent proteins such as enhanced yellow fluorescent protein (EYFP) and Citrine have also been used. However, multicolor imaging with STED is less flexible compared to conventional fluorescence microscopy. To image one fluorophore, STED microscopy requires two lasers at the opposite ends of the absorption and emission spectra of the fluorophore for excitation and depletion, respectively. Therefore, the number of colors that can fit into the visible to near IR spectrum range is limited, and a maximum of two colors has been imaged simultaneously thus far (Donnert et al., 2007; Schmidt et al., 2008). Figure 2C shows a two-color STED image of the mitochondrial outer-membrane protein TOM20 and matrix protein HSP70 obtained using the latter approach (Schmidt et al., 2008).

Three-Dimensional Imaging

The depletion pattern in STED microscopy is created by inserting a spatial light modulator in the laser beam before it enters the microscope. As a ring-shaped pattern in the x-y plane improves the lateral resolution, a pattern having two maxima along the z axis improves the axial resolution (Hell, 2003). Overlaying these two patterns improves the resolution in both lateral and axial directions (Harke et al., 2008), allowing three-dimensional (3D) super-resolution imaging with a z resolution ~2.5 times the xy resolution. Subsequently, isoSTED has been realized with the z depletion pattern generated by two opposing objectives using the 4Pi configuration, reaching a resolution as high as ~30 nm in all three dimensions, as demonstrated by the 3D images of mitochondria as shown in Figure 2C (Schmidt et al., 2008, 2009). Recently, STED microscopy has also been demonstrated with two-photon excitation, allowing super-resolution imaging deep into tissue samples (Ding et al., 2009; Moneron and Hell, 2009).

In SIM, a 3D illumination pattern can be created by the interference of three excitation light beams. Similar to the 2D counterpart, 3D SIM can double the resolution in all three dimensions, resulting in ~100 nm resolution in the lateral directions and ~300 nm in the axial direction (Gustafsson et al., 2008; Schermelleh et al., 2008). Figure 2D shows such a 3D SIM image of the nucleus. This 3D illumination pattern can be further combined with I5M using two-opposing objectives to achieve an isotropic resolution of ~100 nm in all three dimensions (Gustafsson et al., 2008). 3D SSIM has not yet been implemented.

Live Imaging

For imaging dynamic events in living systems, time resolution is critical. In all imaging methods, there is an intrinsic trade-off between spatial and temporal resolution. This trade-off can be best understood from the Nyquist sampling theory. In the case of STED microscopy, which acquires an image by scanning the focal spot across the sample, the Nyquist criterion requires the scanning step size to be smaller than half of the desired resolution. The time resolution is then determined by the integration time per scanning step, the scanning step size, and the size of the imaging field. Better spatial resolution requires a smaller scanning step size and, consequently, a longer time to image the same field of view.

Therefore, to achieve high temporal resolution, a small field of view and a relative large spatial resolution can be used. For example, impressive video rate STED imaging (28 frames per second) with 62 nm spatial resolution has been demonstrated in a field of view of ~5 μm2, allowing the motion of individual synaptic vesicles in a dendritic spine to be followed (Westphal et al., 2007). More recently, replacing the traditional pulsed lasers in STED microscopy with continuous wave lasers permits substantially faster scanning and thus higher time resolution (Willig et al., 2007). Using this scheme, a ~70 μm2 image of the endoplasmic reticulum took only 0.19 s to acquire (Moneron et al., 2010).

To study very fast processes, STED microscopy can be combined with fluorescence correlation spectroscopy (FCS). FCS is traditionally used with confocal microscopy to probe the movement or dynamics of molecules by monitoring the fluctuation of the fluorescence signal. The smaller detection region in STED enables FCS to probe dynamics at a much smaller length scale. Indeed, STED-FCS has been used to monitor diffusion dynamics with microsecond time resolutions and at a length scale as small as 20 nm (Eggeling et al., 2009).

SIM acquires an image by scanning an illumination pattern that covers the entire image field. Thus, the image acquisition time of SIM is limited by how fast the illumination pattern can be modulated and by how fast the camera can read out a snapshot. For 2D SIM, which requires nine different illumination patterns to reconstruct a resolution-enhanced image, a time resolution of ~0.1 s has been demonstrated for live-cell imaging (Kner et al., 2009). Therefore, SIM is excellent for live-cell applications that require a large view field but not very high spatial resolution. Although live-cell imaging has not yet been demonstrated with SSIM, one would expect it to have higher spatial resolution at the cost of lower time resolution, as compared to SIM, because more illumination patterns are required to reconstruct an SSIM image (Gustafsson, 2005).

Super-Resolution Fluorescence Microscopy by Single-Molecule Switching

Localizing Single Molecules with High Precision

Fundamentally, a molecular assembly is fundamentally defined by the coordinates for each of its constituents. Therefore, if we could image each molecule individually and determine its position with high precision, we could reconstruct a high-resolution image of the assembly. After 20 years of development in the field of single-molecule imaging (Moerner, 2007), single fluorophores are now routinely detected in a variety of imaging modalities, such as epifluorescence, total-internal-reflection, confocal, and multiphoton microscopies.

Once each fluorescent probe in a sample can be imaged individually, its positions can be determined to high precision by finding the center of the single-molecule image (Thompson et al., 2002; Yildiz et al., 2003). The uncertainty in determining the molecule's position (i.e., the localization precision) scales approximately with the inverse square root of the number of photons detected from the molecule. It can be approximated by aΔN, wherein Δ denotes the width of the diffraction-limited point spread function and N is the number of photons detected (Thompson et al., 2002). For bright fluorescent dyes, about one million photons can be detected from a single molecule, leading to a localization precision of ≤ 1 nm (Pertsinidis et al., 2010; Yildiz et al., 2003). The relative position of multiple fluorophores can also be determined by taking advantage of different emission colors (Churchman et al., 2005; Lacoste et al., 2000; van Oijen et al., 1998), the sequential photobleaching of individual fluorophores (Gordon et al., 2004; Qu et al., 2004), or the stochastic blinking of quantum dots (Lagerholm et al., 2006; Lidke et al., 2005).

In the literature, the localization precision is often reported as the standard deviation of multiple localization measurements of a single object, whereas both standard deviation and full width at half maximum (=2.35 × standard deviation for a Gaussian distribution) have been used as measures of the resolution. In this Primer, we use full width at half maximum to describe resolution because it illustrates better the closest resolvable separation between objects.

Stochastic Switching Enables Super-Resolution Imaging

Being able to localize a single molecule does not directly translate into super-resolution imaging of a fluorescently labeled biological sample, which can contain thousands of fluorophores inside of the diffraction-limited region. The fluorescence emission from these molecules will overlap severely enough that the overall image appears as a completely featureless blur. At first sight, it might seem impossible to distinguish these molecules individually. However, if the fluorescence emission from these molecules is controlled such that only one molecule is emitting at a time, individual molecules can then be imaged and localized. This is the idea behind a recently developed super-resolution imaging method, called STORM (Rust et al., 2006), PALM (Betzig et al., 2006), and FPALM (Hess et al., 2006).

In this method, photoswitchable (or photoactivatable) fluorophores are used to achieve temporal control of the emission. These fluorophores can be converted between a fluorescent (or “on”) state and a dark (or “off”) state or states that fluoresce at different wavelengths. Therefore, when activation light of a sufficiently low intensity is applied to the sample, only a random, sparse subset of fluorophores is activated to the on state at any time, allowing these molecules to be imaged individually, precisely localized, and then deactivated by switching to a reversible dark state or permanent bleaching. Iterating this process—activation, imaging, and deactivation—then allows the locations of many fluorophores to be mapped and a super-resolution image constructed from these localizations (Figure 3A), either with synchronized activation (Betzig et al., 2006; Hess et al., 2006; Rust et al., 2006) or with asynchronous activation (Egner et al., 2007). The image resolution is then no longer limited by diffraction but instead by how precisely each fluorophore is localized. Using this approach, a lateral image resolution as high as ~20 nm has been achieved (Rust et al., 2006).

Figure 3
Super-Resolution Fluorescence Microscopy by Single-Molecule Switching

Although photoswitching offers the most versatile strategy for achieving the temporal control needed for super-resolution imaging, other strategies can also achieve similar control in certain biological samples, such as through the binding and dissociation of fluorescent molecules (Sharonov and Hochstrasser, 2006). It is possible to obtain subdiffraction limit image resolution even when the single-molecule imaging condition is not rigorously satisfied. When the density of fluorophores is not too high, such that temporal fluctuations of neighboring pixels are distinct, higher-order correlation analysis of the temporal fluctuations of individual pixels can be used to substantially improve the image resolution (Dertinger et al., 2009).

Fluorescent Probes

The requirements of STORM/(F)PALM put several constraints on the fluorescent probes. First, the probes should have a fluorescent state that emits light at a certain range of wavelengths and a “dark” state that does not emit light in this wavelength range. Second, to achieve high precision of localization, the probes should emit a large number of photons before going dark. Third, because only one fluorophore is activated within a diffraction-limited area at any time and the vast majority of fluorophores remain in the dark state, dark state emission should be minimal to ensure high-precision localization of the activated molecule. Finally, because all switchable fluorophores can be spontaneously activated by thermal energy or the imaging laser (as opposed to the activation laser), this spontaneous activation could cause more than one fluorophore in the diffraction limited area to be turned on even without activation light, preventing single-molecule detection. Therefore, a low spontaneous activation rate is also desired.

Despite these requirements, a large number of switchable fluorophores have been used for STORM/(F)PALM imaging (Huang et al., 2009; Patterson et al., 2010). These probes range from organic dyes to fluorescent proteins (Table S1 available online), allowing the labeling of biological samples with a variety of methods. To provide readers with general guidelines for choosing the appropriate fluorophore, we describe the advantages and disadvantages of several switchable fluorophores in the Supplemental Information.

For specific experiments, the decision of whether to use dyes or fluorescent proteins depends on a variety of factors, which are commonly applicable to all fluorescence imaging methods. In terms of labeling, fluorescent proteins are genetically encodable, allowing proteins in living cells to be readily labeled with fluorescent proteins. However, dyes are more versatile for labeling different molecular species, including proteins, nucleic acids, oligosaccharides, and even small molecules. Tagging cellular proteins with dyes is typically achieved using immunofluorescence, which permits endogenously expressed proteins to be labeled but prohibits most live-cell imaging applications. In terms of the optical properties, dyes generally have a significantly higher photon output, allowing higher image resolution than fluorescent proteins (Figure 3D). However, at extremely high image resolution, the bulky size of the antibody (10–15 nm) could limit image resolution when immunofluorescence labeling is used. Therefore, the recently developed hybrid fusion systems provide a promising solution that combines the merits of both genetic encoding and the superior fluorescence properties of organic dyes (Fernández-Suárez and Ting, 2008). In these approaches, the protein of interest is fused to a marker protein or peptide, which in turn exhibits a specific reactivity or affinity to fluorescent dyes with certain reactive groups.

3D Imaging

By determining the position of individual molecules in all three dimensions, super-resolution microscopy based on single-molecule switching can be extended to 3D. The first implementation of this approach uses a simple optical design that takes advantage of astigmatism in which light waves in perpendicular directions have different focal points. Specifically, a cylindrical lens is inserted in the imaging path, such that the shape of a single-molecule image becomes elliptical. This makes it possible to determine the axial position of the molecule from the ellipticity and the lateral position from the center position of the image (Huang et al., 2008).Figure 3B shows 3D images of clathrin-coated pits taken with this approach, resolving the nanomorphology of these structures (Huang et al., 2008). Other implementations have utilized a variety of 3D localization methods, such as capturing defocused images at two different focal planes (Juette et al., 2008), engineering a PSF with a double-helical shape (Pavani et al., 2009), and using a mirror to project the axial view to the lateral direction (Tang et al., 2010). Axial resolutions of 40–70 nm have been reported using these methods.

The highest axial resolution in 3D STORM/(F)PALM is achieved by interferometry using two opposing objectives in a similar fashion to 4Pi microscopy and I5M. Figure 2C shows the clear separation of the ventral and dorsal plasma membrane in a thin protrusion of the cell using this method, demonstrating a z resolution of 10 nm (Shtengel et al., 2009). The imaging depth of this approach is relatively small compared to the PSF-fitting approaches described in the previous paragraph, but sample scanning can increase the imaging depths of all of these approaches. In practice, the imaging depth is limited by spherical aberrations due to refractive index mismatch, scattering by turbid samples, and high-fluorescence background arising from thick samples. The combination of single-molecule switching with two-photon excitation/activation provides a promising solution to the latter two problems (Fölling et al., 2008; Vaziri et al., 2008).

Live-Cell Imaging

As with STED and SIM, there is also a trade-off between spatial and temporal resolution with STORM/(F)PALM imaging. In this case, the image is reconstructed from single-molecule localizations. When specifying the spatial resolution, a Nyquist resolution (defined as twice the average distance between neighboring localizations) should be considered in addition to the localization precision (Shroff et al., 2008). This consideration effectively limits the temporal resolution to the time required to collect enough localizations to give the desired Nyquist resolution.

A time resolution of 25–60 s per frame was obtained when imaging focal adhesion complexes labeled with Eos fluorescent protein (EosFP) at a Nyquist resolution of 60–70 nm (Shroff et al., 2008). Similar spatial and temporal resolutions have been achieved when studying the cytoskeleton structure in bacteria using enhanced yellow fluorescent protein (EYFP) (Biteen et al., 2008). This relatively slow imaging speed is due, in part, to the slow switching of fluorescent proteins to the dark states but also due to the decreasing photon output at high-excitation intensities. The use of brighter and faster-switching organic dyes could potentially increase the imaging speed. Various dyes have been combined with the hybrid fusion systems for live-cell super-resolution imaging (Lee et al., 2010; Testa et al., 2010; Wombacher et al., 2010). The spatiotemporal resolution that can be reached with photoswitchable dyes has yet to be characterized with the Nyquist criteria.

Photoactivation has also been combined elegantly with single-particle tracking to visualize dynamic events of living cells (Hess et al., 2007; Manley et al., 2008). Unlike conventional single-molecule tracking experiments, which require low density of target molecules, the use of photoswitchable probes allows a high density of target molecules to be labeled and tracked. Although it may still take a substantial amount of time to accumulate the large number of localizations required to define a structure with high resolution, the motion and dynamics of the molecules inside of the structures can be obtained with millisecond temporal resolution from these single-molecule traces. This imaging mode greatly extends the power of dynamic single-molecule imaging.

Applications of Super-Resolution Fluorescence Microscopy

Despite its relatively short history, super-resolution fluorescence microscopy has already been applied to many areas of biology, and it is beginning to have an impact on numerous fields. It is not possible to describe all of these applications in this Primer article. Instead, we discuss some representative examples in the areas of cell biology, microbiology, and neurobiology.

In Cell Biology

During the development of super-resolution microscopy techniques, a number of subcellular structures with well-characterized morphological features were frequently chosen as proof-of-principle model systems, including microtubules, actin, clathrin-coated pits, mitochondria, endoplasmic reticulum, and focal adhesion complexes. These studies have not only illustrated the resolving power of the new super-resolution techniques, but they also demonstrated these methods' potential for visualizing molecular structures and interactions in cells.

One particularly promising area of applying super-resolution microscopy techniques is the investigation of plasma membrane proteins and membrane microdomains. These domains are generally too small to be resolved by conventional light microscopy. Indeed, many of the controversies in this field, such as the size and the life time of lipid rafts, are due to the lack of direct observations of these microdomains. Super-resolution microscopy thus offers a powerful tool to resolve these debates.

For example, STED microscopy has resolved individual syntaxin 1 clusters in cells, allowing the quantification of the number of syntaxin molecules per cluster (~90) and cluster size (50–60 nm) (Sieber et al., 2007). In conjunction with diffusion measurements by fluorescence recovery after photobleaching (FRAP) and computer modeling, STED demonstrates that syntaxin self-association and steric repulsion fully explain the size and dynamics of syntaxin clusters. In contrast, the clustering of nicotinic acetylcholine receptors is possibly affected by additional mechanisms, including cholesterol-mediated protein-protein interactions and cytoskeleton membrane-protein interactions. These results provide an explanation for the observation that the depletion of cholesterol influences both short range (~50 nm) and long range (0.5–3.5 μm) organizations of nicotinic acetylcholine receptors (Kellner et al., 2007).

The dynamic nature of membrane components requires live-cell imaging with high time resolution. Photoactivation-facilitated high-density single-particle tracking provides a powerful approach to study these dynamics on millisecond to second time scales. Studies of influenza hemagglutinin (Hess et al., 2007), HIV Gag protein, vesicular stomatitis virus G protein (VSVG) (Manley et al., 2008), and epidermal growth factor receptor (Subach et al., 2009, 2010) have uncovered heterogeneous clustering and diffusion behaviors of these membrane proteins. Complementary to this single-particle tracking approach, STED-FCS probes the diffusion behavior of molecules within a subdiffraction focal spot with microsecond time resolution. Studies with STED-FCS discovered that sphingolipids and GPI-anchored proteins can be briefly (10–20 ms) trapped in cholesterol-associated membrane domains of < 20 nm in size, whereas phosphoglycerolipids exhibit free diffusion (Eggeling et al., 2009).

Super-resolution imaging also allows the visualization of fine structures within membrane organelles. For example, biochemical measurements found that human voltage-dependent anion channels (hVDAC) in mitochondria membranes associate with the cytosolic hexokinase-1 (Neumann et al., 2010). In contrast, two-color STED images of mitochondria revealed that a substantial fraction of hVDAC does not colocalize with the pool of hexokinase-I bound to mitochondria. The STED images also reveal that the three hVDAC subtypes exists in distinct domains on the mitochondria outer membrane. IsoSTED has resolved the cristae in the mitochondria inner membrane (Schmidt et al., 2009), suggesting the potential to study protein interactions in the interior of mitochondria.

STORM/(F)PALM has also provided new insights into the organizations of proteins associated with plasma membranes and intracellular membrane organelles. Three-dimensional super-resolution images taken with this approach resolved the hemispherical shape of the clathrin coat on nascent endocytic vesicles (Figure 3B) (Bates et al, 2007; Huang et al., 2008). In an in vitro endocytosis model system, two-color 3D imaging revealed that the two tubule-forming proteins, dynamin and the F-BAR domain protein FBP17, have distinct distributions along the membrane tubules connecting clathrin-coated pits and the basal plasma membrane (Figure 3B). In addition, STORM/(F)PALM, combined with electron microscopy and time-lapse conventional fluorescence imaging, uncovered an unexpected role of FBP17 in creating endocytic vesicles (Wu et al., 2010). Live-cell imaging with this approach has also revealed interesting dynamics of adhesion complexes during their initiation, maturation, and dissolution (Shroff et al., 2008). A recent 3D super-resolution study revealed a multilaminar molecular architecture of the focal adhesion core region that connects integrin and actin (Kanchanawong et al., 2010). This core region is comprised of an integrin-signaling layer, a force transduction layer, and an actin regulatory layer (Kanchanawong et al., 2010).

Super-resolution fluorescence microscopy is expected to expand our understanding of the structures inside of the nucleus. The highly condensed DNA packaging inside of the nucleus calls for super-resolution imaging with protein and nucleic acid sequence specificity, which is difficult to achieve by any other means. Super-resolution fluorescence microscopy provides the opportunity to resolve the interactions between nucleic acids and proteins inside of the nucleus. For example, studies with 3D SIM revealed morphological changes of the chromosomes and the nuclear lamin during early mitosis (Schermelleh et al., 2008). In addition, STORM/(F)PALM has shown promise in resolving chromatin fibers (Matsuda et al., 2010). We anticipate that, with further improved image resolution and sequence-specific nucleic acid labeling by FISH, DNA/RNA-binding proteins, or aptamers, the regulation of gene expression may be directly visualized inside of the nucleus using super-resolution imaging.

These are only a few examples that illustrate the impact that super-resolution fluorescence microscopy is having on cell biology. We look forward to seeing more questions answered by the rapid adoption of super-resolution microscopy tools, especially given this technique's ability to resolve subcellular structures beyond the organelle level.

In Microbiology

Super-resolution fluorescence microscopy may be the imaging tool for which microbiologists have long been waiting. In bacteria, life processes occur in a small, crowded volume of ~1 um3, in which thousands of different protein and RNA species reside. Our view of bacterial structure has undergone a major transformation in recent years. Instead of being viewed simply as a bag of randomly distributed molecules colliding with each other, we now realize that bacteria contain highly organized chromosomes, dynamic cytoskeletal structures, and specific subcellular regions involved in signaling and biosynthetic processes. However, our understanding of the molecular organization in bacterial cells is still primitive.

This gap in our knowledge stems primarily from the difficulty in imaging such a small cell, with a size not much larger than the diffraction limit of optical resolution. Indeed, under the light microscope, most structures inside of a bacterial cell look like a blur. Though electron microscopy (EM) often comes to the rescue when high image resolution is required, its application to bacterial imaging is rather limited. To obtain molecular-specific contrast with EM, immunogold labeling is typically used, and this labeling approach requires cell fixation and permeabilization. Unfortunately, this fixation and permeabilization process perturbs structures inside of bacteria substantially more than inside of eukaryotic cells. Furthermore, live imaging is so far impossible with EM.

The high molecular specificity offered by various fluorescent labeling approaches and the live-cell compatibility of super-resolution fluorescence microscopy offer an ideal solution to the bacterial imaging problem. Indeed, this approach has been used to study several different protein organizations in bacterial cells. For example, STORM/(F)PALM was used to determined the distributions of the chemotaxis proteins Tar receptor, CheY, and CheW in fixed Escherichia coli cells (Greenfield et al., 2009). These proteins were found to form clusters with exponentially distributed sizes. The cluster locations were consistent with a stochastic self-assembly model that does not require active transport. In Calobactur crescentus, live-cell STORM/(F)PALM imaging of MreB, an actin analog, revealed a helical organization of the protein (Biteen et al., 2008). A similar approach was also used to study the partitioning (Par) apparatus in C. crescentus. These studies found that the ATPase ParA forms a narrow, linear polymer structure that runs through the center of the cell body, functioning in a way that is similar to the mitotic spindles observed in eukaryotic cells (Ptacin et al., 2010).

These early applications of super-resolution imaging to bacterial cells have shown the great promise of the approach. Considering the primitive knowledge that we have of the organization of the chromosomes and proteins in bacteria, we expect super-resolution fluorescence microscopy to transform microbiology in the coming years.

In Neurobiology

Ever since Cajal observed Golgi-stained neurons under light microscopes more than a century ago, we have known that the brain functions by sending information from the axons to the dendrites of neurons. Therefore, a “wiring diagram” detailing how neurons are connected to each other will provide a structural foundation for understanding brain function, as well as its malfunction in neurological disorders. However, such a wiring diagram has not been obtained yet except for Caenorhabditis elegans (White et al., 1986). Why not? The small diameter of neurites (as small as tens of nanometers) and their high packing density require image resolution at the nanometer scale for wire tracing. Moreover, to annotate a wiring diagram, we need to identify the neuronal connections (i.e., the synapses) and characterize their properties. These properties depend on the molecular content of the synapses and thus require imaging with molecular specificity. In fact, synaptic function is orchestrated by an elaborate protein machinery with hundreds of protein species packed together in a structure of submicron size. Understanding synaptic function and plasticity requires a detailed characterization of the organization and dynamics of these molecules at specific synaptic sites. Thus, a method to map neural circuitry needs molecule-specific contrast, nanometer-scale resolution, and, ideally, live tissue imaging capability. Super-resolution fluorescence microscopy uniquely satisfies these requirements.

Indeed, neuroscience is one of the first disciplines to which super-resolution fluorescence microscopy was applied. One productive area of application is the study of subneuronal structures, such as synapses. STED images of the active zone protein Bruchpilot at the neuromuscular junction in Drosophila revealed a donut-shaped distribution centered at the active zones of the synapses. Furthermore, the molecules appear to adopt a preferred orientation along the transynaptic axis (Fouquet et al., 2009; Kittel et al., 2006). Using a similar approach, AMPA receptors have been shown to have a ring-shaped distribution in the ribbon synapses of inner hair cells (Meyer et al., 2009). Studies of synaptotagmin, a component of the synaptic vesicle component, show that these proteins remain clustered after exocytosis (Willig et al., 2006).

STORM/(F)PALM has also been used to investigate the molecular architecture of synapses. A systematic study of ten pre- and postsynaptic proteins in the central synapses of mouse brain tissue revealed oriented organization of the presynaptic scaffolding proteins bassoon and piccolo; compartmental distributions of the postsynaptic proteins PSD95, shank, and Homer; and heterogeneous neurotransmitter distributions, with some synapses showing central-synaptic and others showing perisynaptic receptor distribution (Dani et al., 2010). STORM/(F)PALM is able to count molecules, and this property allowed quantitative analysis of neurotransmitter receptor compositions in synapses (Dani et al., 2010). One recent study adapted photoactivation-facilitated high-density single-particle tracking to study actin dynamics at the dendritic spine and uncovered distinct loci of enhanced actin polymerization (Frost et al., 2010).

In addition to illuminating subcellular structures inside of neurons, super-resolution fluorescence microscopy has also been used to study neuronal morphology. Beautiful STED images revealed the shape and dynamics of dendritic spines in live cells (Nägerl et al., 2008). Two-photon STED allowed the visualization of spine morphology in deep tissue samples (Ding et al., 2009). The application of super-resolution imaging to map neuronal connectivity is also currently under way.

However, determining the complete neuronal wiring diagram may require even higher resolution than what is currently feasible by super-resolution microscopy. Although resolution of a few tens of nanometers is now routinely obtainable using various super-resolution fluorescence microscopy techniques, tracing thin and densely packed axons in the brain may likely require a resolution of a few nanometers. Studying subneuronal structures, such as synapses, could also benefit from substantially higher resolution than the current state of the art. A resolution of a few tens of nanometers allows the organization of molecular assemblies and organelles to be determined, whereas resolution at a few nanometers (i.e., a true molecular scale) would permit the direct visualization of interactions between molecules.

Concluding Remarks

Not that long ago, the diffraction limit was considered insurmountable. In recent years, the invention of various super-resolution techniques has shattered the diffraction barrier and allowed the resolution of images from light microscopy to improve by an order of magnitude over the diffraction limit. In fact, all of these super-resolution approaches have, in principle, made spatial resolution unlimited. That said, we believe that, practically, an additional order of magnitude in resolution improvement is feasible.

It is worth noting that, in addition to the intrinsic optical resolution, which often depends on the brightness and photostability of the fluorescent probes, the effective image resolution is also limited by the labeling density and the size of the fluorescent labels. Therefore, in addition to improvements in the design of microscopes, developments in fluorescent probes and labeling chemistry are also critical for further improving the resolution of light microscopy. Indeed, recent advances in super-resolution fluorescence microscopy have already inspired a large amount of research activity in these areas. We foresee that, together, these efforts will allow live-cell imaging at a truly molecular-scale resolution, thereby enabling the direct visualization of molecular interactions and biochemical events in living cells.

Supplementary Material


This work is in part supported by the Searle Scholarship, Packard Fellowship, and the UCSF Program for Breakthrough Biomedical Research (to B.H.) and the Howard Hughes Medical Institute (Collaborative Innovation Award), National Institutes of Health, and the Gatsby Charitable Foundation (to X.Z.). X.Z. is a Howard Hughes Medical Institute investigator.


Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Supplemental Information Supplemental Information includes Extended Discussion and one table and can be found with this article online at *bxs.


  • Abbe E. Beitrage zur Theorie des Mikroskops und der mikroskopischen Wahrnehmung. Arch. Mikroskop Anat. 1873;9:413–420.
  • Bates M, Huang B, Dempsey GT, Zhuang X. Multicolor super-resolution imaging with photo-switchable fluorescent probes. Science. 2007;317:1749–1753. [PMC free article] [PubMed]
  • Betzig E, Lewis A, Harootunian A, Isaacson M, Kratschmer E. Near-field scanning optical microscopy (NSOM) - Development and biophysical applications. Biophys. J. 1986;49:269–279. [PubMed]
  • Betzig E, Patterson GH, Sougrat R, Lindwasser OW, Olenych S, Bonifacino JS, Davidson MW, Lippincott-Schwartz J, Hess HF. Imaging intracellular fluorescent proteins at nanometer resolution. Science. 2006;313:1642–1645. [PubMed]
  • Biteen JS, Thompson MA, Tselentis NK, Bowman GR, Shapiro L, Moerner WE. Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFP. Nat. Methods. 2008;5:947–949. [PMC free article] [PubMed]
  • Bretschneider S, Eggeling C, Hell SW. Breaking the diffraction barrier in fluorescence microscopy by optical shelving. Phys. Rev. Lett. 2007;98:218103. [PubMed]
  • Churchman LS, Okten Z, Rock RS, Dawson JF, Spudich JA. Single molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time. Proc. Natl. Acad. Sci. USA. 2005;102:1419–1423. [PubMed]
  • Dani A, Huang B, Bergan J, Dulac C, Zhuang X. Super-resolution imaging of chemical synapses in the brain. Neuron. 2010;68:843–856. [PMC free article] [PubMed]
  • Dertinger T, Colyer R, Iyer G, Weiss S, Enderlein J. Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI) Proc. Natl. Acad. Sci. USA. 2009;106:22287–22292. [PubMed]
  • Ding JB, Takasaki KT, Sabatini BL. Supraresolution imaging in brain slices using stimulated-emission depletion two-photon laser scanning microscopy. Neuron. 2009;63:429–437. [PMC free article] [PubMed]
  • Donnert G, Keller J, Wurm CA, Rizzoli SO, Westphal V, Schönle A, Jahn R, Jakobs S, Eggeling C, Hell SW. Two-color far-field fluorescence nanoscopy. Biophys. J. 2007;92:L67–L69. [PubMed]
  • Eggeling C, Ringemann C, Medda R, Schwarzmann G, Sandhoff K, Polyakova S, Belov VN, Hein B, von Middendorff C, Schönle A, Hell SW. Direct observation of the nanoscale dynamics of membrane lipids in a living cell. Nature. 2009;457:1159–1162. [PubMed]
  • Egner A, Geisler C, von Middendorff C, Bock H, Wenzel D, Medda R, Andresen M, Stiel AC, Jakobs S, Eggeling C, et al. Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters. Biophys. J. 2007;93:3285–3290. [PMC free article] [PubMed]
  • Fernández-Suárez M, Ting AY. Fluorescent probes for super-resolution imaging in living cells. Nat. Rev. Mol. Cell Biol. 2008;9:929–943. [PubMed]
  • Fölling J, Belov V, Riedel D, Schönle A, Egner A, Eggeling C, Bossi M, Hell SW. Fluorescence nanoscopy with optical sectioning by two-photon induced molecular switching using continuous-wave lasers. ChemPhysChem. 2008;9:321–326. [PubMed]
  • Fouquet W, Owald D, Wichmann C, Mertel S, Depner H, Dyba M, Hallermann S, Kittel RJ, Eimer S, Sigrist SJ. Maturation of active zone assembly by Drosophila Bruchpilot. J. Cell Biol. 2009;186:129–145. [PMC free article] [PubMed]
  • Frost NA, Shroff H, Kong H, Betzig E, Blanpied TA. Single-molecule discrimination of discrete perisynaptic and distributed sites of actin filament assembly within dendritic spines. Neuron. 2010;67:86–99. [PMC free article] [PubMed]
  • Giepmans BN, Adams SR, Ellisman MH, Tsien RY. The fluorescent toolbox for assessing protein location and function. Science. 2006;312:217–224. [PubMed]
  • Gordon MP, Ha T, Selvin PR. Single-molecule high-resolution imaging with photobleaching. Proc. Natl. Acad. Sci. USA. 2004;101:6462–6465. [PubMed]
  • Greenfield D, McEvoy AL, Shroff H, Crooks GE, Wingreen NS, Betzig E, Liphardt J. Self-organization of the Escherichia coli chemotaxis network imaged with super-resolution light microscopy. PLoS Biol. 2009;7:e1000137. [PMC free article] [PubMed]
  • Gustafsson MGL. Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. J. Microsc. 2000;198:82–87. [PubMed]
  • Gustafsson MGL. Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution. Proc. Natl. Acad. Sci. USA. 2005;102:13081–13086. [PubMed]
  • Gustafsson MGL, Agard DA, Sedat JW. Sevenfold improvement of axial resolution in 3D wide-field microscopy using two objective lenses. Proc. SPIE. 1995;2412:147–156.
  • Gustafsson MGL, Shao L, Carlton PM, Wang CJR, Golubovskaya IN, Cande WZ, Agard DA, Sedat JW. Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination. Biophys. J. 2008;94:4957–4970. [PubMed]
  • Harke B, Ullal CK, Keller J, Hell SW. Three-dimensional nanoscopy of colloidal crystals. Nano Lett. 2008;8:1309–1313. [PubMed]
  • Heintzmann R, Cremer C. Lateral modulated excitation microscopy: improvement of resolution by using a diffraction grating. Proc. SPIE. 1999;3568:185–196.
  • Heintzmann R, Gustafsson MGL. Subdiffraction resolution in continuous samples. Nat. Photonics. 2009;3:362–364.
  • Heintzmann R, Jovin TM, Cremer C. Saturated patterned excitation microscopy—a concept for optical resolution improvement. J. Opt. Soc. Am. A Opt. Image Sci. Vis. 2002;19:1599–1609. [PubMed]
  • Hell SW. Toward fluorescence nanoscopy. Nat. Biotechnol. 2003;21:1347–1355. [PubMed]
  • Hell SW. Far-field optical nanoscopy. Science. 2007;316:1153–1158. [PubMed]
  • Hell SW, Stelzer EHK. Fundamental improvement of resolution with 4Pi-confocal fluorescence microscope using 2-photon excitation. Opt. Commun. 1992;93:277–282.
  • Hell SW, Wichmann J. Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Opt. Lett. 1994;19:780–782. [PubMed]
  • Hess ST, Girirajan TPK, Mason MD. Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. Biophys. J. 2006;91:4258–4272. [PubMed]
  • Hess ST, Gould TJ, Gudheti MV, Maas SA, Mills KD, Zimmerberg J. Dynamic clustered distribution of hemagglutinin resolved at 40 nm in living cell membranes discriminates between raft theories. Proc. Natl. Acad. Sci. USA. 2007;104:17370–17375. [PubMed]
  • Hofmann M, Eggeling C, Jakobs S, Hell SW. Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins. Proc. Natl. Acad. Sci. USA. 2005;102:17565–17569. [PubMed]
  • Huang B, Wang WQ, Bates M, Zhuang XW. Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy. Science. 2008;319:810–813. [PMC free article] [PubMed]
  • Huang B, Bates M, Zhuang X. Super-resolution fluorescence microscopy. Annu. Rev. Biochem. 2009;78:993–1016. [PMC free article] [PubMed]
  • Juette MF, Gould TJ, Lessard MD, Mlodzianoski MJ, Nagpure BS, Bennett BT, Hess ST, Bewersdorf J. Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples. Nat. Methods. 2008;5:527–529. [PubMed]
  • Kanchanawong P, Shtengel G, Pasapera AM, Ramko EB, Davidson MW, Hess HF, Waterman CM. nanoscle arthitecture of integrin-based cell adhesions. Nature. 2010;468:580–584. [PMC free article] [PubMed]
  • Kellner RR, Baier CJ, Willig KI, Hell SW, Barrantes FJ. Nanoscale organization of nicotinic acetylcholine receptors revealed by stimulated emission depletion microscopy. Neuroscience. 2007;144:135–143. [PubMed]
  • Kittel RJ, Wichmann C, Rasse TM, Fouquet W, Schmidt M, Schmid A, Wagh DA, Pawlu C, Kellner RR, Willig KI, et al. Bruchpilot promotes active zone assembly, Ca2+ channel clustering, and vesicle release. Science. 2006;312:1051–1054. [PubMed]
  • Klar TA, Hell SW. Subdiffraction resolution in far-field fluorescence microscopy. Opt. Lett. 1999;24:954–956. [PubMed]
  • Kner P, Chhun BB, Griffis ER, Winoto L, Gustafsson MG. Super-resolution video microscopy of live cells by structured illumination. Nat. Methods. 2009;6:339–342. [PMC free article] [PubMed]
  • Lacoste TD, Michalet X, Pinaud F, Chemla DS, Alivisatos AP, Weiss S. Ultrahigh-resolution multicolor colocalization of single fluorescent probes. Proc. Natl. Acad. Sci. USA. 2000;97:9461–9466. [PubMed]
  • Lagerholm BC, Averett L, Weinreb GE, Jacobson K, Thompson NL. Analysis method for measuring submicroscopic distances with blinking quantum dots. Biophys. J. 2006;91:3050–3060. [PubMed]
  • Lee HL, Lord SJ, Iwanaga S, Zhan K, Xie H, Williams JC, Wang H, Bowman GR, Goley ED, Shapiro L, et al. Superresolution imaging of targeted proteins in fixed and living cells using photoactivatable organic fluorophores. J. Am. Chem. Soc. 2010;132:15099–15101. [PMC free article] [PubMed]
  • Lewis A, Isaacson M, Harootunian A, Muray A. Development of a 500 Å spatial resolution light microscope. Ultramicroscopy. 1984;13:227–231.
  • Lidke KA, Rieger B, Jovin TM, Heintzmann R. Superresolution by localization of quantum dots using blinking statistics. Opt. Express. 2005;13:7052–7062. [PubMed]
  • Lord SJ, Lee HL, Moerner WE. Single-molecule spectroscopy and imaging of biomolecules in living cells. Anal. Chem. 2010;82:2192–2203. [PMC free article] [PubMed]
  • Manley S, Gillette JM, Patterson GH, Shroff H, Hess HF, Betzig E, Lippincott-Schwartz J. High-density mapping of single-molecule trajectories with photoactivated localization microscopy. Nat. Methods. 2008;5:155–157. [PubMed]
  • Matsuda A, Shao L, Boulanger J, Kervrann C, Carlton PM, Kner P, Agard D, Sedat JW. Condensed mitotic chromosome structure at nanometer resolution using PALM and EGFP- histones. PLoS ONE. 2010;5:e12768. [PMC free article] [PubMed]
  • Meyer AC, Frank T, Khimich D, Hoch G, Riedel D, Chapochnikov NM, Yarin YM, Harke B, Hell SW, Egner A, Moser T. Tuning of synapse number, structure and function in the cochlea. Nat. Neurosci. 2009;12:444–453. [PubMed]
  • Moerner WE. New directions in single-molecule imaging and analysis. Proc. Natl. Acad. Sci. USA. 2007;104:12596–12602. [PubMed]
  • Moneron G, Hell SW. Two-photon excitation STED microscopy. Opt. Express. 2009;17:14567–14573. [PubMed]
  • Moneron G, Medda R, Hein B, Giske A, Westphal V, Hell SW. Fast STED microscopy with continuous wave fiber lasers. Opt. Express. 2010;18:1302–1309. [PubMed]
  • Nägerl UV, Willig KI, Hein B, Hell SW, Bonhoeffer T. Live-cell imaging of dendritic spines by STED microscopy. Proc. Natl. Acad. Sci. USA. 2008;105:18982–18987. [PubMed]
  • Neumann D, Bückers J, Kastrup L, Hell SW, Jakobs S. Two-color STED microscopy reveals different degrees of colocalization between hexokinase-I and the three human VDAC isoforms. PMC Biophys. 2010;3:4. [PMC free article] [PubMed]
  • Novotny L, Hecht B. Principles of Nano-optics. Cambridge University Press; Cambridge: 2006.
  • Patterson G, Davidson M, Manley S, Lippincott-Schwartz J. Superresolution imaging using single-molecule localization. Annu. Rev. Phys. Chem. 2010;61:345–367. [PMC free article] [PubMed]
  • Pavani SR, Thompson MA, Biteen JS, Lord SJ, Liu N, Twieg RJ, Piestun R, Moerner WE. Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function. Proc. Natl. Acad. Sci. USA. 2009;106:2995–2999. [PubMed]
  • Pertsinidis A, Zhang Y, Chu S. Subnanometre single-molecule localization, registration and distance measurements. Nature. 2010;466:647–651. [PubMed]
  • Pohl DW, Denk W, Lanz M. Optical stethscopy - Image recording with resolution lembda/20. Appl. Phys. Lett. 1984;44:651–653.
  • Ptacin JL, Lee SF, Garner EC, Toro E, Eckart M, Comolli LR, Moerner WE, Shapiro L. A spindle-like apparatus guides bacterial chromosome segregation. Nat. Cell Biol. 2010;12:791–798. [PMC free article] [PubMed]
  • Qu XH, Wu D, Mets L, Scherer NF. Nanometer-localized multiple single-molecule fluorescence microscopy. Proc. Natl. Acad. Sci. USA. 2004;101:11298–11303. [PubMed]
  • Rittweger E, Han KY, Irvine SE, Eggeling C, Hell SW. STED microscopy reveals crystal colour centres with nanometric resolution. Nat. Photon. 2009;3:144–147.
  • Rust MJ, Bates M, Zhuang XW. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM) Nat. Methods. 2006;3:793–795. [PMC free article] [PubMed]
  • Schermelleh L, Carlton PM, Haase S, Shao L, Winoto L, Kner P, Burke B, Cardoso MC, Agard DA, Gustafsson MGL, et al. Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy. Science. 2008;320:1332–1336. [PMC free article] [PubMed]
  • Schmidt R, Wurm CA, Jakobs S, Engelhardt J, Egner A, Hell SW. Spherical nanosized focal spot unravels the interior of cells. Nat. Methods. 2008;5:539–544. [PubMed]
  • Schmidt R, Wurm CA, Punge A, Egner A, Jakobs S, Hell SW. Mitochondrial cristae revealed with focused light. Nano Lett. 2009;9:2508–2510. [PubMed]
  • Sharonov A, Hochstrasser RM. Wide-field subdiffraction imaging by accumulated binding of diffusing probes. Proc. Natl. Acad. Sci. USA. 2006;103:18911–18916. [PubMed]
  • Shroff H, Galbraith CG, Galbraith JA, Betzig E. Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics. Nat. Methods. 2008;5:417–423. [PubMed]
  • Shtengel G, Galbraith JA, Galbraith CG, Lippincott-Schwartz J, Gillette JM, Manley S, Sougrat R, Waterman CM, Kanchanawong P, Davidson MW, et al. Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure. Proc. Natl. Acad. Sci. USA. 2009;106:3125–3130. [PubMed]
  • Sieber JJ, Willig KI, Kutzner C, Gerding-Reimers C, Harke B, Donnert G, Rammner B, Eggeling C, Hell SW, Grubmüller H, Lang T. Anatomy and dynamics of a supramolecular membrane protein cluster. Science. 2007;317:1072–1076. [PubMed]
  • Subach FV, Patterson GH, Manley S, Gillette JM, Lippincott-Schwartz J, Verkhusha VV. Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nat. Methods. 2009;6:153–159. [PMC free article] [PubMed]
  • Subach FV, Patterson GH, Renz M, Lippincott-Schwartz J, Verkhusha VV. Bright monomeric photoactivatable red fluorescent protein for two-color super-resolution sptPALM of live cells. J. Am. Chem. Soc. 2010;132:6481–6491. [PMC free article] [PubMed]
  • Sun Y, McKenna JD, Murray JM, Ostap EM, Goldman YE. Parallax: high accuracy three-dimensional single molecule tracking using split images. Nano Lett. 2009;9:2676–2682. [PMC free article] [PubMed]
  • Synge EH. A suggested method for extending microscopic resolution into the ultra-microscopic region. Philos. Mag. 1928;6:356–362.
  • Tang J, Akerboom J, Vaziri A, Looger LL, Shank CV. Near-isotropic 3D optical nanoscopy with photon-limited chromophores. Proc. Natl. Acad. Sci. USA. 2010;107:10068–10073. [PubMed]
  • Testa I, Wurm CA, Medda R, Rothermel E, von Middendorf C, Fölling J, Jakobs S, Schönle A, Hell SW, Eggeling C. Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelength. Biophys. J. 2010;99:2686–2694. [PubMed]
  • Thompson RE, Larson DR, Webb WW. Precise nanometer localization analysis for individual fluorescent probes. Biophys. J. 2002;82:2775–2783. [PubMed]
  • van Oijen AM, Kohler J, Schmidt J, Muller M, Brakenhoff GJ. 3-Dimensional super-resolution by spectrally selective imaging. Chem. Phys. Lett. 1998;292:183–187.
  • Vaziri A, Tang J, Shroff H, Shank CV. Multilayer three-dimensional super resolution imaging of thick biological samples. Proc. Natl. Acad. Sci. USA. 2008;105:20221–20226. [PubMed]
  • Westphal V, Lauterbach MA, Di Nicola A, Hell SW. Dynamic far-field fluorescence nanoscopy. N.J. Phys. 2007;9:10.
  • White JG, Southgate E, Thomson JN, Brenner S. The structure of the ventral nerve cord of Caenorhabditis elegans. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 1986;275:327–348. [PubMed]
  • Willig KI, Rizzoli SO, Westphal V, Jahn R, Hell SW. STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis. Nature. 2006;440:935–939. [PubMed]
  • Willig KI, Harke B, Medda R, Hell SW. STED microscopy with continuous wave beams. Nat. Methods. 2007;4:915–918. [PubMed]
  • Wombacher R, Heidbreder M, van de Linde S, Sheetz MP, Heilemann M, Cornish VW, Sauer M. Live-cell super-resolution imaging with trimethoprim conjugates. Nat. Methods. 2010;7:717–719. [PubMed]
  • Wu M, Huang B, Graham M, Raimondi A, Heuser JE, Zhuang X, De Camilli P. Coupling between clathrin-dependent endocytic budding and F-BAR-dependent tubulation in a cell-free system. Nat. Cell Biol. 2010;12:902–908. [PMC free article] [PubMed]
  • Xie XS, Choi PJ, Li GW, Lee NK, Lia G. Single-molecule approach to molecular biology in living bacterial cells. Annu. Rev. Biophys. 2008;37:417–444. [PubMed]
  • Yildiz A, Forkey JN, McKinney SA, Ha T, Goldman YE, Selvin PR. Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization. Science. 2003;300:2061–2065. [PubMed]
  • Zipfel WR, Williams RM, Webb WW. Nonlinear magic: multiphoton microscopy in the biosciences. Nat. Biotechnol. 2003;21:1369–1377. [PubMed]