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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Neurosci. Author manuscript; available in PMC Jun 7, 2012.
Published in final edited form as:
PMCID: PMC3270580
NIHMSID: NIHMS347943
Sleep spindles in humans: insights from intracranial EEG and unit recordings
Thomas Andrillon,1,2* Yuval Nir,1* Richard J. Staba,3 Fabio Ferrarelli,1 Chiara Cirelli,1 Giulio Tononi,1 and Itzhak Fried3,4
1Department of Psychiatry, University of Wisconsin-Madison, 6001 Research Park Blvd, Madison, WI, 53719, USA
2Department of Cognitive Studies, École Normale Supérieure, 29 rue d’Ulm, 75005 Paris, France
3David Geffen School of Medicine, University of California-Los Angeles, 710 Westwood Plaza, Los Angeles, CA 90095, USA
4Functional Neurosurgery Unit, Tel Aviv Medical Center and Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
Corresponding author: Yuval Nir, PhD, University of Wisconsin – Madison, School of Medicine, Department of Psychiatry, 6001 Research Park Blvd, Madison, WI 53719, ynir/at/wisc.edu
*contributed equally to this work.
Sleep spindles are an electroencephalographic (EEG) hallmark of non-rapid eye movement (NREM) sleep and are believed to mediate many sleep-related functions, from memory consolidation to cortical development. Spindles differ in location, frequency, and association with slow waves, but whether this heterogeneity may reflect different physiological processes and potentially serve different functional roles remains unclear. Here we utilized a unique opportunity to record intracranial depth EEG and single-unit activity in multiple brain regions of neurosurgical patients to better characterize spindle activity in human sleep. We find that spindles occur across multiple neocortical regions, and less frequently also in the parahippocampal gyrus and hippocampus. Most spindles are spatially restricted to specific brain regions. In addition, spindle frequency is topographically organized with a sharp transition around the supplementary motor area between fast (13-15Hz) centroparietal spindles often occurring with slow wave up-states, and slow (9-12Hz) frontal spindles occurring 200ms later on average. Spindle variability across regions may reflect the underlying thalamocortical projections. We also find that during individual spindles, frequency decreases within and between regions. In addition, deeper sleep is associated with a reduction in spindle occurrence and spindle frequency. Frequency changes between regions, during individual spindles, and across sleep may reflect the same phenomenon, the underlying level of thalamocortical hyperpolarization. Finally, during spindles neuronal firing rates are not consistently modulated, although some neurons exhibit phase-locked discharges. Overall, anatomical considerations can account well for regional spindle characteristics, while variable hyperpolarization levels can explain differences in spindle frequency.
Sleep spindles constitute an EEG hallmark of NREM sleep (Loomis et al., 1935; Andersen and Andersson, 1968; Achermann and Borbely, 1998; Destexhe and Sejnowski, 2001; De Gennaro and Ferrara, 2003; Steriade, 2003). In humans, spindles are classically defined as waxing-and-waning 10-16Hz oscillations lasting 0.5-2s (Gibbs, 1950). Comparable sleep spindles have been found in all tested mammalian species (Zepelin et al., 1994) and similar phenomena occur during barbiturate anesthesia (Contreras et al., 1997c) and in vitro (McCormick and Bal, 1997).
The neurophysiological mechanisms of spindle generation reflect the intrinsic properties and interactions between inhibitory cells in the thalamic reticular nucleus (RE) and bursting thalamocortical (TC) relay neurons (Steriade, 2000; Steriade, 2003). The GABAergic neurons in the highly interconnected thalamic RE act as pacemakers and are both necessary and sufficient for spindle generation (Morison and Bassett, 1945; Steriade et al., 1985; Steriade et al., 1987; Marini et al., 1992; Bal et al., 1995; Destexhe et al., 1998; Steriade and Timofeev, 2003; Halassa et al., 2011). Spindle waxing is attributed to gradual cell recruitment in RE-TC-RE loops (Steriade et al., 1993b; von Krosigk et al., 1993). The source of spindle waning and termination is less clear but possibly involves the depolarizing action of the thalamic IH current and/or corticothalamic influence (Bal et al., 1995; Luthi and McCormick, 1998; Timofeev et al., 2001; Bonjean et al., 2011). Spindle synchronization is controlled by neocortical feedback, as decortication or spreading depression lead to asynchronous spindles (Contreras and Steriade, 1996; Contreras et al., 1997c).
Increasing evidence suggests that sleep spindles in humans are a diverse rather than prototypical phenomenon. One distinction involves fast (13-15Hz) centroparietal versus slow (11-13Hz) frontal spindles (Gibbs, 1950; Anderer et al., 2001; Schabus et al., 2007). Another distinction is between spindles that occur on a background of low-amplitude EEG in stage 2 sleep and are easily identified visually (Iber et al., 2007), and spindles associated with slow waves (Steriade et al., 1993a; Molle et al., 2002). The functional role of spindles remains unclear, although they may play a role in memory consolidation (Schabus et al., 2004), cortical development (Khazipov et al., 2004), regulation of arousal (Destexhe and Sejnowski, 2002), and in maintaining a disconnection from the external environment through a “thalamic gate” (Steriade, 2003). In addition, spindles have been recently explored as a potential biomarker for psychiatric disorders such as schizophrenia (Ferrarelli et al., 2010). To be able to identify the role(s) spindles could play during normal states of vigilance and to exploit them as a biomarker for pathology, it is necessary to first better understand spindle properties through a comprehensive investigation of their characteristics when they occur most prominently, i.e. human sleep. To this aim, here we characterize in detail sleep spindles in humans using simultaneous recordings of intracranial depth EEG and unit spiking activities in multiple brain regions in the cortex and hippocampus of thirteen individuals undergoing presurgical localization of pharmacologically resistant epilepsy.
Subjects and polysomnographic sleep studies
Thirteen patients (6 males, 7 females) with pharmacologically intractable epilepsy (ages 19-52) underwent monitoring with depth electrodes (Figure 1A) for seizure foci identification and potential surgical treatment (Fried et al., 1999). Patients provided written informed consent to participate in the research study, under the approval of the Medical Institutional Review Board at UCLA. Electrode location was based only on clinical criteria, and all surgery was performed by Itzhak Fried.
Figure 1
Figure 1
Data overview and spindle detection
Sleep recordings were conducted 48-72 hr after surgery, at a minimal interval of 12 hours from identifiable seizures, and lasted for about 7 hours between 2300 and 0600. In addition to continuous video monitoring, the montage included two electrooculogram (EOG), two electromyogram (EMG), and four scalp electrodes (positioned at C3, C4, Pz, Fz) as well as two earlobe electrodes for referencing. Sleep-wake stages were scored according to established guidelines (Iber et al., 2007).
Data Acquisition
Electrode location varied between patients based on their clinical profiles. Electrodes were placed in frontal, parietal, and cingulate cortices, as well as temporal lobe regions such as hippocampus, amygdala, entorhinal cortex and parahippocampal gyrus (Figure 1B). For each patient, 8-12 flexible polyurethane depth electrodes (1.25 mm diameter) were implanted orthogonal to the lateral surface of the skull with the distal tip of each electrode typically positioned in medial limbic brain structures. Each electrode position was identified using post-implant computed tomography (CT) co-registered with pre-implant magnetic resonance (MR) imaging (Brain Navigator, Grass-Telefactor Corp., Philadelphia, PA). Scalp and intracranial depth EEG data were continuously recorded, sampled at 2kHz, bandpass-filtered in hardware between 0.1Hz and 500Hz and re-referenced offline to the mean signal of the earlobes electrodes.
Each depth electrode terminated in a set of eight insulated 40-μm platinum-iridium microwires (impedances 200 to 500 kΩ) (Fried et al., 1999) (Figure 1A). Microwire signals were simultaneously recorded continuously (Cheetah Recording System; Neuralynx, Tucson, AZ for 10 patients; Neuroport Recording System; Blackrock, Salt Lake City, UT for 3 patients), sampled at 28 kHz (10 patients) or 30 kHz (3 patients), band-pass filtered in hardware between 1Hz and 9kHz, and referenced locally to a ninth non-insulated microwire.
Detection of spindles and their separation from pathological events
Spindles were detected automatically following previously published algorithms (Clemens et al., 2007; Ferrarelli et al., 2007) based on a sequential three-step process (see Figure 1E-G) implemented in Matlab (MathWorks) as follows. First, to minimize false detections, only channels with robust spindle activity in NREM sleep were chosen for further analysis. In each individual channel, spindle (9-16Hz) power in NREM sleep was compared with a fitted 1/f α model (both were estimated across multiple 10s epochs) and channels with a difference that was statistically significant at p < 0.001 (unpaired t-test for maximal peak) were further considered (Figure 1E). Second, putative spindles were selected based on their power and duration. EEG signals were band-pass filtered between 9 and 16Hz (-3dB at 8.8 and 17.3Hz) using a zero-phase 4th order Butterworth filter. The instantaneous amplitude was computed via the Hilbert transform and two thresholds were defined based on this amplitude time-course across artifact-free sleep epochs. A detection threshold was set at mean + 3SD and amplitudes exceeding this threshold were considered potential spindles. A start/end threshold was set at mean + 1SD and events whose duration was between 0.5s and 2s were further considered (Figure 1F). Detections within 1s were merged as single events. Third, since transient events can introduce power increases across a wide range of frequencies including the spindle range (De Gennaro and Ferrara, 2003), only events in which power increases were specific to the spindle range rather than broadband were chosen for final analysis. For each channel, power of detected events was compared via an unpaired t-test with power of random 1s segments in NREM sleep (Figure 1G). Channels exhibiting weaker diffuse broadband power increases (p >= 0.0001 at maximal difference) were excluded. We further verified the spectral specificity of each spindle by excluding any detection that coincided with control events that were above mean+5SD in the 20-30Hz range.
Sleep spindles were separated from pathological interictal epileptiform events as follows. As was recently done for slow waves (Nir et al., 2011), epileptiform interictal spikes were separately detected automatically in depth EEG by high-pass filtering offline above 100Hz and by identifying events whose amplitude exceeded mean + 5SD and whose duration was under 70ms (de Curtis and Avanzini, 2001). We verified visually that such automatic detection was effective in identifying interictal spikes in each channel separately. Based on this detection we discarded post-hoc any putative spindles within ±1s of interictal spikes. In addition, the parameters of the spindle detection algorithm (e.g. frequency range, filter settings, thresholds) were optimized after extensive visual inspection to minimize false detections of epileptiform activities as spindles, although a wide range of parameters yielded similar detections (not shown). Naturally, the density and duration of spindles reported throughout the paper depend on the specific detection parameters employed.
In the hippocampus, we visually selected for further analysis a subset of the automatically detected events that also exhibited a clear waxing-and-waning spindle shape. We computed in each hippocampal electrode separately the probability of such spindles to co-occur with detected events in other locations as p = number of concomitant spindles for a given pair of structures / number of hippocampal spindles.
Timing and frequency of spindles
For each detected spindle, several parameters were stored for further analysis including start and end times, frequency, and amplitude. The timing of individual spindles was determined as the mean of their start and end times (center), thereby avoiding influence by the choice of the start/end threshold.
The frequency of each detected spindle was estimated by computing the spectrogram in 10s intervals (short-time Fourier transform, 744ms window, 95% overlap) and identifying the maximal power for each event within the spindle frequency range with a resolution of 0.2Hz. Next, distribution of spindle frequencies were computed per channel and later averaged for each brain region (Figure 2B).
Figure 2
Figure 2
Fast centroparietal spindles differ from slow frontal spindles
Spectral dynamics during individual spindles in specific regions (e.g. Figure 2C) were evaluated by computing spectrograms (as above) in 2s windows centered on the time of maximal amplitude. Differences in frequency between the beginning and end of spindles (e.g. Figure 2C) were evaluated in each event separately by comparing the instantaneous frequency (in spectrograms) between start and end times via a paired t-test (α=0.05).
Comparing timing and frequency across regions
For each pair of channels in the same individual, concomitant spindles were selected if there was temporal overlap in their occurrence. Both channels were considered as the “seed” and “target” alternatively and spectrograms (as above) were aligned to the time of maximal amplitude in the seed channel (e.g. Figure 3C). For each spindle event separately, the timing of maximal amplitude and the frequency at maximal amplitude were compared across the two channels and the mean difference in timing and frequency across spindles was computed for each pair (Figure 3). Next, electrode locations in frontal and parietal locations were grouped into ten regions: bilateral orbitofrontal cortices, anterior cingulate cortices, pre-supplementary motor areas, supplementary motor areas, and posterior cingulate cortices (Figure 3B). In twelve individuals in whom there was more than one such location, the typical delay between all pairs of spindles for any given pair of regions (e.g. left orbitofrontal cortex vs. right posterior cingulate cortex) were averaged (edge color in Figure 3B). Furthermore, each region was ranked in each individual from 0 (earliest detection) to 1 (latest detection) and such ranks were similarly averaged across individuals (node colors in Figure 3B). We also examined whether the timing differences between regions (pooled in 50ms bins) were proportional to frequency differences via linear regression (Figure 3D).
Figure 3
Figure 3
Fast centroparietal spindles precede slow frontal spindles
Association of spindles with slow waves
Individual slow waves (positive and negative peaks in the EEG filtered below 4Hz that were separated by 0.25s to 1s) were automatically detected as described previously (Riedner et al., 2007) and separated from abnormal paroxysmal events (Nir et al., 2011). The highest amplitude waves in each channel (top 20%) were used to examine spindle occurrence ±2s around down-states (depth positive and surface negative peaks) or up-states (depth negative and surface positive peaks) in 100ms bins (Figure 4B). Confidence intervals (α=0.05) were estimated by computing the mean and SD of spindle occurrence across the 4s detection interval and significant deviations from chance were identified via a two-tailed t-test for each time bin separately.
Figure 4
Figure 4
Association of spindles with slow wave up-states
Local sleep spindles
For each detected spindle, we examined whether other channels recorded a spindle that temporally overlapped with the spindle in the “seed” channel, using each channel as seed in turn. The presence or absence of coincident spindles between channels was labeled ‘concordant’ or ‘non-concordant’, respectively (Figure 5B). In both seed and target regions we computed the spectral dynamics (spectrograms as above) in ± 1sec intervals aligned to the peak spindle power in the seed channel. Spectrograms were normalized in each channel separately relative to power modulations in random intervals with no detected spindles in the seed channel (Sirota et al., 2003). Statistical comparison of spindle power in concordant and non-concordant cases was carried out via a t-test based on peak power. To determine the percent of cases in which “entirely local” spindles occurred (i.e. seed channel showed detected spindle while nothing different from chance occurred in a target channel), we reduced our detection criteria (above) to include spindles that exceeded a confidence interval of 95% (rather than mean + 3SD).
Figure 5
Figure 5
Local sleep spindles
Dynamics of spindles throughout sleep
In a subset of five individuals exhibiting a clear homeostatic decline of slow wave activity (SWA) during sleep, early and late NREM sleep were analyzed separately. We compared the density, frequency, amplitude, and locality of spindles between early and late NREM sleep via non-parametric Mann-Whitney U-tests (α=0.01) separately for different spindle categories (e.g. all spindles, fast vs. slow, frontal and centroparietal, see Figure 6A). The dynamics of spindle frequency within NREM sleep cycles was further examined. To this end, NREM sleep cycles were identified visually as continuous time intervals in NREM sleep preceding REM episodes (N=16 cycles in 8 individuals). Figure 6B shows four of these NREM episodes (the third one being discarded since it is disrupted by a brief arousal). To compile results across individuals and cycles that varied in their duration, each cycle was divided into shorter epochs (n=10 bins per sleep cycle) and the mean SWA and spindle parameters were computed for each bin (Figure 6C). Bins were sorted based on their SWA and the frequency and density of spindles was compared across varying depths of sleep (as indicated by SWA levels).
Figure 6
Figure 6
Deep sleep and high SWA are associated with lower spindle frequencies
Spiking activities during spindles
Units were identified using the ‘wave_clus’ software package (Quiroga et al., 2004) as described previously (Nir et al., 2008). Briefly, a threshold was set at 5SD above the median noise level of the high-pass filtered signal (above 300Hz), detected events were clustered using superparamagnetic clustering, and categorized as noise, single- or multi-unit clusters. Classification of putative single-units and multi-unit clusters was based on the consistency of action potential waveforms, and by the presence of a refractory period for putative single units, i.e. less than 1% of inter-spike-intervals (ISIs) within 3ms. The stability of unit recordings was confirmed throughout long (~7h) sleep recordings by comparing action potential waveforms and ISI distributions of detected units separately in 1 hour intervals, and verifying that both waveforms and ISI distributions were indeed consistent and separable between different clusters throughout the night.
Neuronal action potential discharges recorded on individual microwires were examined in relation to sleep spindles detected in the depth EEG (macro-electrode ~4mm away) in the same brain structure (see Figure 1A), as was recently done for slow waves (Nir et al., 2011). To quantify changes in firing rate during spindles, action potentials were binned in 100ms bins ±2s around the middle of each spindle. Confidence intervals (α=0.05) were estimated across the 4s interval and significant deviations from chance were identified via a paired t-test for each bin separately (Figure 7A).
Figure 7
Figure 7
Unit discharges during sleep spindles
Phase-locked unit discharges during spindles (Figure 7B) were examined as follows. Depth EEG was filtered in the spindle (9-16Hz) range and individual spindles were detected as above. For each spindle, we extracted the instantaneous phase of the filtered EEG via the Hilbert transform. We examined the distribution of phase values at action potential times (“real distribution” in Figure 7C) and computed a p-value using Rayleigh’s test for nonuniformity (‘circ_mean’ function, Circular Statistics Toolbox for Matlab). Since non-uniformity can be partially attributed to asymmetries in the EEG waveforms (Siapas et al., 2005), the critical p-values for significance were determined using bootstrapping for each unit separately as the 95th percentile after examining p-values across 1,000 iterations while shuffling action potential timings within each segment (“shuffled spikes” in Figure 7C). Finally, the preferred phase was determined for each phase-locked unit.
Statistical Analysis
Error bars in figures denote standard error of the mean (SEM = SD/√(n-1). Student T-tests were performed after confirming normal distributions via Kolmogorov-Smirnov tests or the Mann-Whitney U-test (non-parametric) when normality could not be established.
Polysomnographic sleep studies
Polysomnographic sleep studies were conducted in 13 neurosurgical patients with intractable epilepsy. Continuous overnight recordings lasted 421 ± 20 minutes (mean ± SEM). Polysomnography included electrooculogram (EOG), electromyogram (EMG), scalp EEG, and video monitoring. Figure 1A-D summarizes the experimental recording setup during sleep. Sleep-wake stages were scored following established guidelines as waking, NREM sleep stages 1 through 3 and REM sleep (Iber et al., 2007). Intracranial depth EEG was recorded in 129 medial brain regions bilaterally in frontal and parietal cortices as well as multiple limbic structures in the medial temporal lobe (Figure 1B). While it should be noted that our sampling was mostly limited to medial brain areas, scalp topography suggests that human spindle activity is maximal at midline regions (Ferrarelli et al., 2007), thereby making our data particularly well suited to examine sleep spindles. We simultaneously recorded scalp EEG, depth EEG, and spiking activity from a total of 600 units (355 putative single units, 245 multiunit clusters).
Measures of overnight sleep in patients were in general agreement with typical findings in healthy young adults (Riedner et al., 2007). Sleep efficiency, time spent in different sleep stages, NREM-REM sleep cycles, and power spectra of scalp EEG resembled normal sleep characteristics. Moreover, in every subject, power spectra of scalp EEG data in NREM sleep (Figure 1C) revealed robust slow wave activity (<4Hz) and spindle (9-16Hz) power. These results indicate that sleep measures were similar to those of normal sleep in individuals without epilepsy.
Spindle occurrence across multiple regions in the human brain
Having characterized sleep using standard non-invasive polysomnography, we proceeded to identify individual spindles in NREM sleep in the scalp EEG and in the depth EEG of each brain region separately using an automatic algorithm (see Methods and Figure 1E-G). Channels with robust spindle activity in NREM sleep (Figure 1E) in which power increases of detected events were specific to the spindle range rather than broadband (Figure 1G) were identified. Table 1 provides the results of spindle detection across multiple brain regions. In the scalp EEG, spindles were robustly observed (90 ±4.7% of electrodes showed spindle detections; mean ± SEM across individuals) and their density (1.9 ±0.13 spindles/min) was consistent with densities reported in healthy individuals (Wei et al., 1999; Ferrarelli et al., 2010). Spindle density was significantly higher during sleep stage N2 than N3 (1.9 ±0.14 vs. 1.6 ±0.14 spindles/min respectively, p < 10E-3 via paired t-test, n = 46 channels). In the depth EEG, frontal and parietal cortices exhibited robust spindle detections (82% and 89% respectively) with densities comparable to those observed in scalp EEG (1.8 ±0.16 and 1.3 ±0.29 spindles/min, respectively).
Table 1
Table 1
Spindle occurrence across multiple regions in the human brain Columns (left to right) show the brain region, the number of channels with spindle detections (over the total number of electrodes in that region), and the mean density of spindles per minute (more ...)
Spindle occurrence was further examined in the medial temporal lobe (MTL), where the presence of physiological spindles is still debated (Malow et al., 1999; Nakabayashi et al., 2001; Pare et al., 2002). The current results indicate reliable spindle occurrence in the parahippocampal gyrus (PHG, 69% of channels; 1.3 ±0.25 spindles/min) and hippocampus (44% of channels; 1.1 ±0.13 spindles/min), while spindle occurrence was lower in entorhinal cortex and amygdala (31% and 21% of channels; 0.74 ±0.11 and 0.89 ±0.18 spindles/min, respectively). Given the variability in spindle occurrence in the MTL that could be due in part to epileptogenicity, we analyzed spindles in MTL separately from spindles in frontal and parietal cortices, and present data from the MTL in the final section of the results.
Fast centroparietal spindles differ from slow frontal spindles
Since scalp EEG studies have long suspected a distinction between slow (11-13Hz) and fast (13-16Hz) spindles (Gibbs, 1950; Anderer et al., 2001; De Gennaro and Ferrara, 2003; Schabus et al., 2007), we computed the distribution of frequencies across all spindles in each channel separately across multiple brain regions (see Methods). In scalp EEG, centroparietal electrodes (C3, C4, Pz) showed a significant albeit diffuse dominance of fast spindles, whereas slow spindles prevailed in frontal derivations (Fz). Average spindle frequencies in Pz and Fz were 12.6 ±0.23 Hz vs. 11.3 ±0.30 Hz, respectively (mean ± SEM across individuals) and these differences were statistically significant (p < 0.01, non-parametric Mann-Whitney U-test across 24 channels). Next, the mean frequency of spindles was mapped across individual depth electrodes (Figure 2A). In contrast to the small, yet statistically significant, differences in frequency observed with scalp EEG, the intracranial results reveal a topographical organization with a clear difference between fast (>12.5Hz) centroparietal spindles and slow (<12.5Hz) frontal spindles. “Centroparietal” was defined as brain areas from the SMA proper back to posterior cingulate and parietal cortex, although some of these locations are in the frontal lobe. The distributions of spindle frequencies were averaged within and compared between the following five regions: orbitofrontal cortex (OF), anterior cingulate cortex (AC), pSMA, SMA, and posterior cingulate cortex (PC). The results (Figure 2B) confirm the distinction between slow and fast spindles captured in part by scalp EEG. Furthermore, a difference between adjacent pSMA and SMA was observed in the same patient, and it nearly reached statistical significance across patients despite the limited sample (10.9 ±0.72Hz vs. 12.6 ±0.19Hz respectively, p = 0.057, non-parametric Mann-Whitney U-test, n=7 channels) supporting the notion of two spindle groups rather than a continuous cortical gradient (see also Discussion). In addition, while frontal spindles were exclusively slow, centroparietal spindles had a bimodal distribution with a majority of fast spindles and fewer slow spindles, and this was also evident in the data of individual channels.
We also found that in the same brain region, frequency decreases during individual spindles. Figure 2C shows the average frequency dynamics in a representative SMA electrode (fast spindles, left) and an OF electrode (slow spindles, right). As can be seen, the instantaneous frequency of fast SMA spindles dropped on average from 13.8 to 12.4Hz, and slow OF spindles showed a similar deceleration (from 11.5 to 10.4Hz on average). A quantitative analysis across the entire dataset (2,851 fast and 10,607 slow spindles in 50 channels) revealed a significant decrease of −0.8Hz/s in instantaneous frequency between spindle start and end times (-0.8 ±0.04Hz/s and −0.8 ±0.02Hz/s for fast and slow spindles, respectively, p < 10E-12 between start and end times via two-tail t-test).
Fast centroparietal spindles precede slow frontal spindles
To further understand the distinction between fast centroparietal and slow frontal spindles, we examined whether these occurred independently or with consistent temporal relations. Figure 3A shows examples of individual spindles recorded simultaneously across several brain regions. As can be seen, fast centroparietal spindles occurred before slow frontal spindles, and the successive temporal occurrence across channels was accompanied by a decrease in spindle frequency. Next, a quantitative analysis of time offsets across all pairs of brain regions was conducted and the order in which spindles appeared was computed (Figure 3B). The results indicate that centroparietal spindles (in SMA and PC) occurred before slow frontal spindles (pSMA, AC, and OF) with a time difference of 203 ±16ms (mean ± SEM across 2,024 pairs of spindles, p < 10E-12 via one-tail t-test). Sorting regions according to the order in which their spindles were detected revealed that centroparietal regions typically preceded frontal locations (Figure 3B). In contrast, within centroparietal regions and within frontal regions no significant time differences were found, supporting the notion of a discontinuous transition in timing and frequency between fast centroparietal and slow frontal spindles (see Discussion). Figure 3C provides an example of the average timing relation between all spindle pairs (n=139) detected concomitantly in the PC and AC in one individual. As can be seen, timing and frequency differences appear closely linked. Indeed, across the entire dataset timing and frequency of spindles were found to be tightly related in pairs of regions such that larger frequency deceleration was associated with longer time delays (Figure 3D, r = −0.90, n = 9,741 spindles). Overall, the reduction in spectral frequency with respect to time delay between spindles was −1.3 ±0.5Hz/s, and this negative rate was comparable to that found for spindles within the same regions (-0.8 ±0.02Hz/s, see previous section).
Association of spindles with slow wave up-states
Since spindles are often associated with slow wave up-states (Steriade et al., 1993a; Steriade and Amzica, 1998; Molle et al., 2002), we examined how this association may vary for different spindles detected across different brain structures. We have recently characterized in detail slow waves and underlying unit activities in the same dataset (Nir et al., 2011). Here we used the same automatic detection of large slow waves in each individual channel and examined the occurrence of spindles around positive and negative peaks in depth EEG, corresponding to down and up states, respectively. Figure 4A depicts an example of an individual slow wave detected in the anterior cingulate followed by a spindle, whereas Figure 4B presents the quantitative analysis of spindle detections in relation to slow waves across the entire dataset. Higher spindle occurrence (Figure 4B, right) was found in the 0-500ms interval following negative peaks in depth EEG (up states), in line with previous reports (Steriade et al., 1993a; Molle et al., 2002).
Next, association of spindles with slow wave up-states was compared between different brain regions in the 1s interval immediately following the transition to up-states when spindle occurrence was maximal (Figure 4C). Fast centroparietal spindles were found to be more tightly associated with up states compared with slow frontal spindles. Spindles exhibited the shortest delay with up-states in the SMA, the region where spindles were also detected earliest. More generally, typical time differences between regions were maintained for spindles associated with slow wave up-states. Specifically, the average delays from negative peaks in the depth EEG to maximal spindle density in the SMA and PC were 267 ±11ms and 363 ±11ms, respectively, while frontal regions exhibited longer delays (AC, 460 ±8ms; OF, 425 ±6ms; pSMA, 449 ±14ms). The mean difference in delays between centroparietal and frontal sites was statistically significant (p < 10E-12, unpaired two-tailed t-test). Thus, the tighter association between centroparietal spindles and slow wave up-states is consistent with their earlier occurrence compared with slow frontal spindles (see also Discussion).
Local sleep spindles
Although concomitant spindles were observed across multiple recording sites (above), we recently found that both sleep spindles and slow waves often occur independently in separate brain regions (Nir et al., 2011). We present these findings here as well to place them in the context of a comprehensive examination of sleep spindles in the human brain. Examination of local vs. simultaneous spindles was carried out only in cortical sites that had regular spindle occurrences, thereby excluding the possibility that local occurrence of spindles arises merely from their total absence in remote brain structures. Numerous examples of local sleep spindles occurring in specific brain regions were found (Figure 5A). Local spindles occur without spindle activity in other regions, including homotopic regions across hemispheres and regions with equivalent signal-to-noise ratio (SNR) showing global slow waves. We set out to quantitatively establish to what extent local sleep spindles occur across the entire dataset. We determined for each spindle in a given region whether spindles were present or not in other brain structures (Methods). The spectral power changes in concordant sites (45% of cases where a spindle was detected in both “seed” and “target” channels, Figure 5B top row) were compared with those at non-concordant sites (55% of cases where a spindle was detected in the seed channel but not in the target, Figure 5B bottom row). The results revealed a clear difference between peak spectral power values of concordant and non-concordant conditions across spindles in target channels (p < 10E-48, paired t-test), indicating significant differences in underlying neuronal activity, and that non-concordant cases are indeed local spindles. Furthermore, the analysis of those cases where target channels did not exhibit any increase in spindle spectral power above the noise level (Methods) revealed that 32% of all non-concordant cases were local in the strongest sense, i.e. a full-fledged spindle was detected in the seed channel while spectral power in the target channel was not different from chance. Importantly, the occurrence of local spindles was independent of local slow waves, since “isolated” spindles without a slow wave within ± 1.5s (35% of all spindles) were likewise mostly local (Figure 5C). In addition, comparing homotopic regions revealed that 36 ±2% of spindles were observed only in one hemisphere (mean ± SEM across 12 pairs), indicating that differences between anterior and posterior regions could not account for spindle locality.
Next, we quantified the involvement of multiple regions in spindles by computing the number of brain areas in which spindles were observed. The majority of sleep spindles involved a limited number of brain regions (Figure 5C), indicating that local spindles were more frequent than global spindles. Mean involvement in individual spindles was 48 ±0.7% of monitored brain regions (mean ± SEM across 49 depth electrodes), indicating that most spindles were predominantly local. Finally, the spatial extent of spindles correlated with spindle amplitude (Figure 5D, r = 0.52, p < 0.0001, n = 212).
Deep sleep is associated with lower spindle frequencies
The variability in spindle spectral frequency between brain structures and during individual spindles described in the preceding paragraphs could be due to changes in levels of thalamocortical polarization. Since the degree of thalamic hyperpolarization dictates the period of spindle oscillations (McCormick and Bal, 1997; Steriade, 2003), we hypothesized that spindles during deep sleep (early in the night or in the middle of NREM cycles when SWA is highest), would contain lower spectral frequencies than spindles during lighter sleep (late in the night or closer to REM transitions when SWA is lower).
To examine this possibility, we first compared spindle frequencies in five individuals showing a typical homeostatic decline of SWA, i.e. a progression from deep early sleep with powerful slow waves to lighter sleep late in the night. Spindle frequency was significantly lower in early deep sleep when SWA was highest (Figure 6A, reduction of 0.63 ± 0.12Hz, mean ± SEM across 16 channels, p = 0.0004, non-parametric Mann-Whitney U-test). The modulation of spindle frequency in early vs. late sleep was specific to slow spindles (p = 0.002 for slow frontal spindles vs. p = 0.62 for fast spindles, Mann-Whitney U-test).
Along the same line, spindle frequency changed within NREM cycles in accord with changes in SWA. Figure 6B shows an example of dynamics in spindle frequency and SWA across sleep in one individual, illustrating that in the middle of each NREM cycle when SWA was highest, spindle frequency was lowest. Next, this relation was examined in all NREM cycles followed by REM epochs (16 cycles in 29 depth EEG channels and 8 individuals, see Methods). Each NREM cycle was divided into 10 intervals with equal duration and SWA, spindle frequency and spindle density were computed separately for each interval (Figure 6C). Both spindle frequency and spindle density were negatively correlated with SWA during NREM episodes (r= −0.81, p = 0.005 and r = −0.73 p = 0.02). These results indicate that deeper sleep and higher SWA are associated with lower spindle frequencies and fewer events.
Neuronal discharges during sleep spindles
Given that spiking activity across the human brain is tightly locked to EEG slow waves (Nir et al., 2011) we examined whether cortical neurons consistently modulated their firing rate during spindles. Examination of firing rates in putative single-units from individual subjects revealed little evidence for consistent modulations in relation to spindles. On average across all 207 cortical units, we found that spindles occurring within 500ms of transitions from inactive to active (UP) periods (8.5% of events) were accompanied by robust firing rate modulations (Figure 7A, left), as expected (Nir et al., 2011). In contrast, when considering all spindles (n=63,724) firing rate modulations were largely absent (Figure 7A, right), although a small (~4%) yet significant reduction in neuronal discharges was observed 100-400ms following the middle point of spindles, likely reflecting a diffuse tendency for an inactive (DOWN) state to occur at that time.
Next, we evaluated the phase-locking of unit discharges during spindles to determine if units preferably fire at a specific phase of spindle oscillations (Figure 7B). Figure 7C presents an example unit in which spikes preferably occurred at a particular phase (-30 degrees) during spindles. Overall, 19.5% of neurons showed significant phase locking (p < 0.05, see Methods) with a tendency to fire more at the ascending phase of the spindle oscillation (preferred phase = −35 ±8.5 degrees, SEM across units) as measured with depth EEG (Figure 7D).
On average, phase-locked units had a higher firing rate than the average firing rate (5.9 ±1.2Hz vs. 3.1 ±0.3Hz, p = 0.004, Mann-Whitney U-test for putative single units). It could be argued that the firing of many neurons is phase-locked during spindles, but only high firing rate neurons allow enough statistical power to reveal this phenomenon. However, 46% of phase locked neurons had firing rates that were below the mean rate across the entire population. Alternatively, higher firing rates in phase-locked units may reflect a bias towards specific cell types such as interneurons (see also Discussion).
Spindles in the Medial Temporal Lobe (MTL)
Spindle occurrence in the MTL and its possible relation to pathology in epilepsy patients is a matter of continued debate (Malow et al., 1999; Nakabayashi et al., 2001; Pare et al., 2002). Our results (Table 1) revealed reliable spindle occurrence in the PHG, a dependable albeit lower occurrence in the hippocampus, and lower spindle occurrence in the entorhinal cortex and amygdala. Could such events reflect epileptiform activity? To address this, spindle detections in MTL were further analyzed in relation to the seizure onset zone (SOZ) based on the medical records of the patients. We examined whether inclusion in the epileptic focus (25 of 64 MTL channels) could predict spindle detection and spindle density in each region separately. If spindles recorded in MTL solely reflect abnormal activity, then it might be expected that the number of channels and rates of spindles would be higher inside than outside the SOZ, spindles in hippocampus would occur more regularly with spindles in adjacent MTL than remote neocortical sites, or unit firing would be more strongly correlated with MTL than neocortical spindles. Analysis found that in PHG and hippocampus, the majority of channels where spindles were recorded were outside the SOZ (64% and 63% respectively), and there was a trend for higher rates of hippocampal spindles outside than inside the SOZ (1.1 vs. 0.8 spindles/min outside and inside the SOZ, respectively). In the entorhinal cortex and amygdala, spindle occurrence was comparable within and outside the SOZ. In addition, we found that detected events in hippocampus (Methods) co-occur more often with frontal/parietal spindles (remote from the SOZ) than with events detected in amygdala or entorhinal cortex (p < 0.05, Mann-Whitney U-test). Finally, analysis did not find a stronger correlation between unit discharges and spindles in MTL than neocortical sites. It should be noted that due to possible volume conduction and lack of clear firing rate modulations our results cannot resolve the long-lasting controversy of potential epileptiform nature of hippocampal spindles. Nevertheless, these results indicate that although MTL spindles are less frequent and more variable, PHG and hippocampus can exhibit physiological spindles that may transcend possible links to pathology.
Sleep spindles and epilepsy
Our data were recorded in medicated epilepsy patients in whom epileptiform events during seizure-free periods (i.e. interictal episodes) may affect sleep, and sleep spindles in particular (Dinner and Lüders, 2001; Steriade, 2005). Therefore, it was imperative to confirm that our results could be generalized to the healthy population, and multiple observations strongly suggest that this is the case. First, overnight recordings were carried out prior to routine tapering of anti-epileptic drugs to ensure a less significant contribution of epileptiform activities. Second, sleep measures were within the expected normal range, including distribution of sleep stages, NREM-REM cycles, and EEG power spectra of each sleep stage. Third, we specifically detected paroxysmal discharges and separated them from physiological sleep spindles, and we also confirmed that pathological events were not falsely detected as spindles through extensive visual inspection of each individual’s data (Methods). Fourth, the occurrence rate of paroxysmal discharges was highly variable across channels, limited in its spatial extent, and entirely absent in some channels. By contrast, all the results reported here could be observed in every individual despite highly idiosyncratic clinical profiles. Fifth and most importantly, previous analysis revealed significant firing rate modulations in the same neurons during paroxysmal discharges (Nir et al., 2011), whereas unit discharges were not robustly modulated during sleep spindles, attesting to a good separation between pathological and physiological spindles. Since interictal EEG spikes and sleep spindles may be confounded in a more complex manner in the MTL, we adopted a conservative approach and analyzed those spindles separately from data obtained in frontal and parietal cortices based on a visual detection. Overall, we are confident that the current results can be generalized to individuals without epilepsy.
Spindle occurrence and thalamic projections
Spindles were observed most frequently in neocortical regions, less often in the hippocampus and parahippocampal gyrus, and rarely in amygdala and entorhinal cortex (Malow et al., 1999; Nakabayashi et al., 2001; Pare et al., 2002; Sirota et al., 2003; Clemens et al., 2011). Such regional differences are consistent with the extent of thalamic projections to these structures. For example, many thalamic nuclei that are reciprocally connected with the RE project robustly to frontal and parietal cortices where spindles are abundant. By contrast, dorsal thalamic nuclei projecting to the MTL (where spindles occur less frequently) are not reciprocally connected with the RE in cats (Steriade et al., 1984; Pare et al., 1987; Jones, 2007). The lack of spindles in amygdala is consistent with sparse thalamocortical projections that only target the lateral nucleus (Jones, 2007). The difference in spindle occurrence between the hippocampus and entorhinal cortex is less clear and could reflect variability in recording from different layers in these structures.
Slow and fast spindles
Our intracranial results confirm and refine the distinction between slow (9-12Hz) frontal and fast (13-16Hz) centroparietal spindles (Loomis et al., 1935; Jankel and Niedermeyer, 1985; Jobert et al., 1992; Anderer et al., 2001; De Gennaro and Ferrara, 2003; Nakamura et al., 2003; Clemens et al., 2011). We find that (a) this distinction is present in each individual (b) spindle frequency shows a sharp transition between SMA and pSMA, (c) centroparietal spindles show a bimodal distribution whereas frontal spindles are exclusively slow, (d) frontal spindles often have frequencies as low as 9-10Hz.
What gives rise to slow and fast spindles? Since spindles arise from RE-TC-RE loops, and because TC projections from different nuclei terminate in cortical regions that exhibit slow vs. fast spindles, differences in spindle frequency could reflect the preserved topographical organization between thalamic nuclei and sub-regions in the RE sheet (Guillery and Harting, 2003; Steriade and Timofeev, 2003; Jones, 2007; Lam and Sherman, 2011). Posterior and lateral dorsal nuclei project to centroparietal cortex, whereas ventral and anterior dorsal nuclei mainly project to prefrontal regions (Jones, 2007; Cappe et al., 2009; Zhang et al., 2010). Some anterior nuclei project diffusely along the cingulate cortex (Jones, 2002) and could explain occasional slow centroparietal spindles. Finally, the difference in spindle frequency between SMA and pSMA suggests that these adjacent cortical regions receive input from different thalamic nuclei (Akkal et al., 2007).
Faster spindle frequencies are associated with earlier time onsets
Fast centroparietal spindles precede slow frontal spindles with a significant difference between adjacent SMA and pSMA, as was the case for spindle frequency. Differences in spindle timing and frequency are correlated across regions in individual spindles. Furthermore, within each brain region spindles decelerate such that frontal spindles show a hint of an early fast component aligned with centroparietal spindles and vice versa. Thus, individual spindles exhibit different frequencies and timings in distinct regions.
Differences in spindle timing and frequency were noted previously, but their close relation during individual spindles had yet to be established (Zygierewicz et al., 1999; Anderer et al., 2001; Nakamura et al., 2003; Clemens et al., 2011; Dehghani et al., 2011). Earlier studies suggested that timing differences among cortical spindles reflect propagation along the RE rather than through intracortical pathways (Kim et al., 1995; Contreras et al., 1997c). Accordingly, spindles may propagate rostrally along the RE, progressively innervating different dorsal nuclei which project topographically to the cortex, generating spindles with different frequencies at different times in different locations.
Sleep spindles are mostly local
Spindles have been traditionally regarded as global thalamocortical events, but local spindles were reported during cortical spreading depression and barbiturate anesthesia (Andersen and Andersson, 1968; Contreras et al., 1996, 1997c; Contreras et al., 1997b). The current results demonstrate that most sleep spindles occur locally in natural sleep as do slow waves (Nir et al., 2011), and that local spindle occurrence does not simply reflect associations with slow waves or distinctions between slow and fast events. These findings suggest that spindles may occur or be regulated locally (Caderas et al., 1982; Nishida and Walker, 2007; Dehghani et al., 2010a; Halassa et al., 2011) and support the notion that sleep arises from activities of local circuits (Werth et al., 1997; Vyazovskiy et al., 2000; Huber et al., 2004; Esser et al., 2006; Huber et al., 2006; Krueger et al., 2008; Nir et al., 2011). It is an open question whether local and global spindles may be mediated by different mechanisms such as laminar-specific corticothalamic projections, or thalamocortical projections via the core and matrix cells (Zikopoulos and Barbas, 2007; Jones, 2009; Rubio-Garrido et al., 2009).
The relation of spindles with sleep slow waves
With respect to slow waves, spindles tend to occur preferentially at up-states (Molle et al., 2002; Steriade, 2003). Such association has also been termed K-complex but this terminology is still under debate (Steriade and Amzica, 1998; Cash et al., 2009). Centroparietal spindles tend to occur shortly after the transition to up-states. Since up-states are believed to trigger spindles through corticothalamic projections, centroparietal cortex may exert particularly powerful corticothalamic feedback. Given that slow waves propagate from prefrontal cortex towards posterior regions (Massimini et al., 2004; Nir et al., 2011), and spindles occur sooner in posterior regions (above), the net result is that frontal spindles occur later during slow wave up-states.
Our results extend the notion of reciprocal relation between SWA and spindle frequency and density (Steriade and Amzica, 1998; Wei et al., 1999; Himanen et al., 2002; De Gennaro and Ferrara, 2003). In stage N3 when SWA is maximal, spindle density is reduced. In early NREM sleep when SWA is highest, spindle density and spindle frequency are significantly lower. Moreover, spindle density and frequency are lower in the middle of NREM cycles when SWA is maximal and higher towards the transition to REM sleep when SWA tapers off.
Unit discharges during sleep spindles
We found that firing rate modulations of cortical neurons during spindles are weak and unreliable compared with those observed during slow waves (Nir et al., 2011), largely consistent with previous reports (Evarts, 1964; Steriade et al., 1974; Sirota et al., 2003). This seems at odds with suggestions that sleep spindles play an important role in disconnection from the external environment or in memory consolidation (Diekelmann and Born, 2010) but perhaps specific neuronal populations are more strongly modulated during spindles (Dehghani et al., 2010b). Another possibility is that inhibition in NREM sleep may occur concomitantly with spindle-driven post-synaptic potentials in dendrites and prevent reliable spike firing (Contreras et al., 1997a).
In addition, we found that spindles affected spike timing in cortical neurons. The discharges of 20% of neurons were significantly phase-locked, firing maximally in the ascending slope of spindles in the depth EEG as in animal studies (Contreras and Steriade, 1996; Sirota et al., 2003). Interpretation of entrained neurons and their preferred phase should consider that unit discharges were recorded ~4mm away from depth EEG and their laminar profiles remain unknown, which could explain minor differences with other species (Sirota et al., 2003; Dehghani et al., 2010b). That spike timing is modulated by sleep spindles and is also implicated in plasticity processes (Caporale and Dan, 2008) supports the notion that spindles may have a role in synaptic plasticity (Khazipov et al., 2004). A trend for higher firing rates in phase-locked units could suggest that specific cell types (e.g. inhibitory interneurons) preferentially modulate their spike timing with spindles, but our data could not resolve this intriguing possibility.
Spindle density and frequency as reflections of thalamic hyperpolarization
We suggest that the level of thalamic hyperpolarization may be a key factor governing multiple spindle properties such as their spectral frequency, density, regional variability, dynamics during individual events, and across sleep. In TC cells, the degree of hyperpolarization dictates the period of spindle oscillations (McCormick and Bal, 1997; Steriade, 2003). Along this line, we find that deeper sleep (higher SWA and presumably stronger thalamic hyperpolarization) is associated with lower frequency cortical spindles. In addition, at the level of individual thalamic neurons, spindles and slow waves arise at different hyperpolarization levels and are mutually exclusive (Nunez et al., 1992). Indeed, we find that high SWA is associated with fewer spindles, presumably since strong hyperpolarization reduces the recruitment of thalamic neurons that support spindle generation. Regional differences in spindle density and frequency may stem from differential hyperpolarization levels in specific thalamic sub-regions. For example, posterior thalamic nuclei (projecting to centroparietal cortices) receive stronger cholinergic innervations (Jones, 2007). Alternatively, intrinsic cellular properties may differ regionally in thalamic and RE neurons (e.g. distributions of neurotransmitter receptors or ionic channels). Finally, deceleration during each spindle could reflect increasing hyperpolarization. Cortical neurons are increasingly more involved in spindle termination (Contreras et al., 1996; Steriade, 2003), and these corticothalamic projections are believed to exert an overall inhibitory influence through their strong influence on RE cells (Golshani et al., 2001). If this hypothesis is correct, a simple non-invasive EEG measure of spindle frequency could provide an important proxy for the level of thalamocortical polarization at any given time.
Acknowledgements
We thank the patients for their cooperation; E. Behnke, T. Fields, A. Tankus, N. Suthana and K. Shattuck for assistance with data acquisition and software; B. Salaz and I. Wainwright for administrative help; S. Sarasso, V. Vyazovskiy and B. Hung for discussions. This work was supported by the Human Frontier Science Program Organization long-term fellowships (Y.N.), the Brainpower for Israel Fund (Y.N.), the NIH Director’s Pioneer Award (G.T.), and NINDS grants (R.S., I.F.)
Footnotes
Conflict of interest: None to declare
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