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Nucleic Acids Res. 1982 November 11; 10(21): 6797–6808.
PMCID: PMC326965

Identification of splicing signals in introns of yeast mitochondrial split genes: mutational alterations in intron bI1 and secondary structures in related introns.


Four mitochondrial mutations are known to block excision of intron I1 of the cob gene in S.cerevisiae. The nucleotide sequence alteration of one of them, M4873, has been determined. It is a deletion of 1 bp in a run of five G's at a distance of 30 to 34 bp upstream to the 3' splice point. Reversion is found to occur by restoration of the run of five G's either by insertion of 1 G (wild type reversion) or by transition A leads to G next to this run of G's (pseudo-wild type reversion). The effect of mutation and reversion on RNA splicing indicates that the run of five G's is of critical importance for intron I1 excision, possibly in participating in the formation of a splice signal with a helical structure. This presumption is confirmed by the observation that this sequence is part of a larger sequence of some 80 bp next to the 3' splice point which is conserved to some extend in the four mitochondrial introns (bI1, aI1, aI2, aI5) that survive after excision as circular RNAs. Most striking is the conservation of this sequence at the level of secondary structure.

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Selected References

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  • De La Salle H, Jacq C, Slonimski PP. Critical sequences within mitochondrial introns: pleiotropic mRNA maturase and cis-dominant signals of the box intron controlling reductase and oxidase. Cell. 1982 Apr;28(4):721–732. [PubMed]
  • Lewin B. Alternatives for splicing: recognizing the ends of introns. Cell. 1980 Nov;22(2 Pt 2):324–326. [PubMed]
  • Halbreich A, Pajot P, Foucher M, Grandchamp C, Slonimski P. A pathway of cytochrome b mRNA processing in yeast mitochondria: specific splicing steps and an intron-derived circular DNA. Cell. 1980 Feb;19(2):321–329. [PubMed]
  • Lazowska J, Jacq C, Slonimski PP. Sequence of introns and flanking exons in wild-type and box3 mutants of cytochrome b reveals an interlaced splicing protein coded by an intron. Cell. 1980 Nov;22(2 Pt 2):333–348. [PubMed]
  • Schmelzer C, Schweyen RJ. Evidence for ribosomes involved in splicing of yeast mitochondrial transcripts. Nucleic Acids Res. 1982 Jan 22;10(2):513–524. [PMC free article] [PubMed]
  • Bechmann H, Haid A, Schweyen RJ, Mathews S, Kaudewitz F. Expression of the "split gene" COB in yeast mtDNA. Translation of intervening sequences in mutant strains. J Biol Chem. 1981 Apr 10;256(7):3525–3531. [PubMed]
  • Bailey JM, Davidson N. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Anal Biochem. 1976 Jan;70(1):75–85. [PubMed]
  • Heyting C, Meijlink FC, Verbeet MP, Sanders JP, Bos JL, Borst P. Fine structure of the 21S ribosomal RNA region on yeast mitochondria DNA. I. Construction of the physical map and localization of the cistron for the 21S mitochondrial ribosomal RNA. Mol Gen Genet. 1979 Jan 11;168(3):231–246. [PubMed]
  • Alwine JC, Kemp DJ, Stark GR. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350–5354. [PubMed]
  • Rigby PW, Dieckmann M, Rhodes C, Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. [PubMed]
  • Schmelzer C, Haid A, Grosch G, Schweyen RJ, Kaudewitz F. Pathways of transcript splicing in yeast mitochondria. Mutations in intervening sequences of the split gene COB reveal a requirement for intervening sequence-encoded products. J Biol Chem. 1981 Jul 25;256(14):7610–7619. [PubMed]
  • Van Ommen GJ, Boer PH, Groot GS, De Haan M, Roosendaal E, Grivell LA, Haid A, Schweyen RJ. Mutations affecting RNA splicing and the interaction of gene expression of the yeast mitochondrial loci cob and oxi-3. Cell. 1980 May;20(1):173–183. [PubMed]
  • Bonitz SG, Coruzzi G, Thalenfeld BE, Tzagoloff A, Macino G. Assembly of the mitochondrial membrane system. Structure and nucleotide sequence of the gene coding for subunit 1 of yeast cytochrme oxidase. J Biol Chem. 1980 Dec 25;255(24):11927–11941. [PubMed]
  • Arnberg AC, Van Ommen GJ, Grivell LA, Van Bruggen EF, Borst P. Some yeast mitochondrial RNAs are circular. Cell. 1980 Feb;19(2):313–319. [PubMed]
  • Schweyen RJ, Weiss-Brummer B, Backhaus B, Kaudewitz F. The genetic map of the mitochondrial genome in yeast: map positions of drug' and mit- markers as revealed from population analyses of rho- clones in Saccharomyces cerevisiae. Mol Gen Genet. 1978 Feb 16;159(2):151–160. [PubMed]

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