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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Neuron. Author manuscript; available in PMC 2013 January 26.
Published in final edited form as:
PMCID: PMC3269037
NIHMSID: NIHMS346595

Endocannabinoids shape accumbal encoding of cue-motivated behavior via CB1 receptor activation in the ventral tegmentum

SUMMARY

Transient increases in nucleus accumbens (NAc) dopamine concentration are observed when animals are presented with motivationally salient stimuli and are theorized to energize reward seeking. They arise from high frequency firing of dopamine neurons in the ventral tegmental area (VTA), which also results in the release of endocannabinoids from dopamine cell bodies. In this context, endocannabinoids are thought to regulate reward seeking by modulating dopamine signaling, although a direct link has never been demonstrated. To test this, we pharmacologically manipulated endocannabinoid neurotransmission in the VTA while measuring transient changes in dopamine concentration in the NAc during reward seeking. Disrupting endocannabinoid signaling dramatically reduced, whereas augmenting levels of the endocannabinoid 2-arachidonoylglycerol (2AG) increased, cue-evoked dopamine concentrations and reward seeking. These data suggest that 2AG in the VTA regulates reward seeking by sculpting ethologically relevant patterns of dopamine release during reward-directed behavior.

INTRODUCTION

The neural mechanisms responsible for the pursuit of rewards in the environment are essential for the survival of the organism (Nesse and Berridge, 1997; Schultz et al., 1997). Environmental stimuli that predict the availability of reward develop incentive-motivational properties that energize the seeking of future rewards (Bindra, 1968). The NAc is a neural substrate that is critically involved in integrating interoceptive and environmental information with emotional information to initiate reward seeking (Kelley, 1999; Mogenson et al., 1980). When reward seeking is maintained in a controlled experimental setting in which environmental stimuli predict reward-availability, transient dopamine surges in the NAc begin to occur in response to the predictive stimuli (i.e., conditioned cues) following the attribution of incentive salience (Berridge and Robinson, 1998; Flagel et al., 2011). These transient increases in dopamine have been detected in the NAc when animals are exposed to cues predicting various rewards – including drugs of abuse (Phillips et al., 2003), food (Roitman et al., 2004), and brain stimulation reward (Cheer et al., 2007a) – and are required to promote reward-directed behavior (Nicola, 2010).

The brain endocannabinoid system, formed by metabotropic cannabinoid receptors (CB1 and CB2) and their endogenous ligands (e.g. anandamide and 2AG), is important for the regulation of dopamine signaling during reinforcement processing (Lupica and Riegel, 2005; Solinas et al., 2008). When dopamine neurons in the VTA exhibit brief high frequency firing episodes they release endocannabinoids that act as retrograde messengers by binding to pre-synaptic CB1 receptors, thereby indirectly modulating the excitability of dopamine neurons by reducing pre-synaptic neurotransmitter release (Melis et al., 2004). Rather than being released through a vesicular mechanism, endocannabinoids are distinct from other neurotransmitters in that they are formed and released “on demand” during specific neural events (Freund et al., 2003). It is likely, therefore, that endocannabinoids regulate dopamine signaling during reward seeking exclusively in situations in which dopamine neurons fire at high frequencies – like when animals are presented with environmental cues predicting reward (Schultz et al., 1997).

To investigate whether endocannabinoids modulate the neural mechanisms of reward seeking, we measured changes in the concentration of cue-evoked dopamine transients in the NAc shell while pharmacologically altering endocannabinoid signaling during operant behavior. A pharmacological approach was chosen because we previously demonstrated that blocking CB1 receptors using rimonabant (a CB1 receptor antagonist) reduced drug-induced transient dopamine release into the NAc (Cheer et al., 2007b). Operant behavior was maintained by either brain stimulation reward or food reinforcement while an environmental cue signaled the availability of reward. We found that disrupting endocannabinoid signaling uniformly decreased the concentration of cue-evoked dopamine transients and reward seeking. These findings prompted us to investigate whether increasing endocannabinoid levels would facilitate reward seeking, and if so, which endocannabinoid is responsible. Using recently developed pharmacological tools designed to manipulate specific components of the endocannabinoid system, we found that augmenting 2AG, but not anandamide, levels by disrupting metabolic enzyme activity increased dopamine signaling during reward seeking – suggesting that 2AG sculpts ethologically relevant patterns of dopamine release during reward-directed behavior.

RESULTS

Transient dopamine concentrations time-locked to cue presentation develop across trials

Dopamine was measured in the NAc shell using fast-scan cyclic voltammetry (FSCV) while responding was maintained in a previously described intra-cranial self-stimulation (ICSS) task (Cheer et al., 2007a). As in our previous report (Cheer et al., 2007a), a compound cue predicted reward availability. This occurred across multiple sensory modalities; specifically, a house light turned off, an ongoing tone ceased, and then 1-s later a white stimulus light mounted above the lever was presented simultaneously with lever extension. A 10-s timeout followed each lever response. Under these conditions, electrically-evoked dopamine release occurred following a lever response and was temporally dissociable from cue-evoked dopamine release events, allowing for changes in the concentration of cue-evoked dopamine to be measured across trials. In agreement with previous studies (Day et al., 2007; Owesson-White et al., 2008), the concentration of dopamine occurring in response to the cue during this acquisition session increased across trials (Figure 1A, 1B). While the concentration of cue-evoked dopamine rapidly increased (Figure 1C; R2=0.85; n=5), the latency to respond from lever extension (a metric of reward seeking) decreased in a linear fashion (Figure 1D; R2=0.80; n=5; mean values: 7.18, 7.16, 6.91, 6.81-s), demonstrating that the strengthening of Pavlovian associations between the cue and unconditioned stimulus is accompanied by increased and cue-related dopamine signaling (Day et al., 2007). Importantly, increased recruitment of endocannabinoids in the VTA should develop in association with an increasing concentration of cue-evoked dopamine release. As dopamine neurons fire in high frequency bursts, voltage gated CA2+ ion channels open and the resulting CA2+ influx activates the enzymes responsible for the synthesis of endocannabinoids (Wilson and Nicoll, 2002). Thus, endocannabinoid levels should be highest in the VTA after periods of phasic dopamine neural activity. If endocannabinoids are indeed involved in modulating dopamine signaling during reward seeking, pharmacological disruption of endocannabinoids should decrease cue-evoked dopamine concentrations and cue-motivated responding in unison.

Figure 1
Reward seeking and cue-evoked dopamine release events are strengthened across trials

Disrupting endocannabinoid signaling during ICSS decreases cue-evoked dopamine concentrations and reward seeking

To assess the effects of disrupting endocannabinoid signaling on cue-evoked dopamine concentrations and reward seeking, we treated rats with the CB1 receptor antagonist rimonabant while responding was maintained by brain stimulation reward in an ICSS task. Following the establishment of stable baseline concentrations of cue-evoked dopamine release, animals were given access to 30 stimulations for each component of the session (i.e., baseline, vehicle and drug treatment). A high (0.3 mg/kg i.v.; MWU test, U=3, p<0.01; n=15; mean values: b=0.91, v=1.09, rimo=2.45-s) but not low (0.125 mg/kg i.v.) rimonabant dose increased the latency to respond for brain stimulation reward (Figure 2A) in comparison to vehicle treatment. The increase in response latency was accompanied by a decrease in the concentration of cue-evoked dopamine (Figure 2B; F(2,44)=5.40, p<0.01; 0.3 mg/kg versus vehicle, p=0.02; also see Supplemental Figure 1A for mean dopamine concentration traces). Cue-evoked dopamine concentrations were not effected by the lower rimonabant dose (Figure 2B; 0.125 mg/kg i.v.). Representative color plots and accompanying dopamine concentration traces (Figure 2C) show rimonabant (0.3 mg/kg i.v.) decreasing cue-evoked dopamine events during individual trials, whereas the representative surface plot (Figure 2D) illustrates the effect of rimonabant (0.3 mg/kg i.v.) on dopamine concentrations across trials. We further determined that the decreases in reward seeking and cue-evoked dopamine concentration could not be explained by a drug-induced effect on electrically-evoked dopamine release (Supplemental Figure 1B), consistent with an absence of CB1 receptors on dopamine terminals (Julian et al., 2003), and could be replicated using the more selective CB1 receptor antagonist AM251 (Supplemental Figure 1C). Next we sought to establish if disrupting the VTA endocannabinoid system alone is sufficient to decrease dopamine neurotransmission by infusing rimonabant directly into the VTA during reward seeking maintained in the ICSS task. As was found following systemic treatment, intrategmental rimonabant (200 ng i.c., unilateral) significantly increased the latency to respond for brain stimulation reward (Figure 2E; MWU test, U=0, p<0.01; n=8; mean values: b=0.94, v=1.10, rimo=1.96-s) and decreased cue-evoked dopamine concentrations (Figure 2F; F(2,14)=7.01, p<0.01; 200 ng versus vehicle, p=0.03; also see Supplemental Figure 4A for mean dopamine concentration traces). The representative dopamine concentration traces (Figure 2G) show the effect of intrategmental rimonabant on cue-evoked dopamine events in individual trials. Rimonabant-induced decreases in cue-evoked dopamine concentration during reward seeking maintained in the ICSS task can also be observed in audio-visual format (Supplemental Video 1). These data demonstrate that the VTA endocannabinoid system modulates dopamine signaling during the pursuit of brain stimulation reward.

Figure 2
Disrupting endocannabinoid signaling decreases cue-evoked dopamine concentrations during reward seeking in an ICSS task

Disrupting endocannabinoid signaling during food self-administration decreases cue-evoked dopamine concentrations and appetitive food seeking

To assess whether disrupting endocannabinoid signaling also decreases dopamine transmission during the pursuit of natural reward, we treated animals with rimonabant while responding was maintained in an appetitive food-seeking task (Supplemental Methods). Similar to the ICSS task, each lever response resulted in the delivery of food reinforcement and retraction of the lever for 10-s. After each 10-s time-out, a compound cue indicating reward availability was presented prior to lever extension. Rimonabant decreased food seeking, as both a low (0.125 mg/kg IV; MWU test, U=4, p=0.03; n=6) and high (0.3 mg/kg IV; MWU test, U=0, p<0.01; n=8; mean values: b=1.45, v=1.82, rimo=17.7-s) dose increased response latency in comparison to vehicle treatment (Figure 3A). Rimonabant was administered prior to 60 responses, before animals reached satiety levels (avg. of 200 reinforced responses). As in the ICSS task, an increase in response latency was accompanied by a decrease in the concentration of cue-evoked dopamine release (Figure 3C; F(2,14)=5.87, p<0.01; 0.3 mg/kg versus vehicle, p=0.04; also see Supplemental Figure 2A for mean dopamine concentration traces). Rimonabant-induced decreases in cue-evoked dopamine concentration during individual (Figure 3D) and repeated (Figure 3E) trials are illustrated in pseudo-color. Likewise, intrategmental rimonabant-induced increases in response latency (Figure 3F; MWU test, u=0, p<0.01; n=5; mean values: b=1.18, v=1.3, rimo=2.75-s) were accompanied by a decrease in cue-evoked dopamine concentration (Figure 3G; F(2,14)=9.86, p<0.01; 200 ng versus vehicle, p=0.014; also see Supplemental Figure 4B for mean dopamine concentration traces). Representative traces showing the effects of vehicle and intrategmental rimonabant (200 ng i.c., unilateral) on cue-evoked dopamine events are illustrated in Figure 3H. Rimonabant-induced decreases in food seeking can also be observed by viewing audio-visual material (Supplemental Video 2). Together, these data demonstrate that disrupting the VTA endocannabinoid system alone is sufficient to decrease natural reward seeking.

Figure 3
Disrupting endocannabinoid signaling decreases cue-evoked dopamine neurotransmission during appetitive food seeking

Cannabinoid receptors are abundantly expressed throughout the central and peripheral nervous system however, and are known to regulate consummatory behavior at a systems level (Gomez et al 2002; Berry and Mechoulam et al 2002). We therefore tested whether rimonabant-induced decreases in food seeking can be explained by a decrease in consummatory behavior rather than a decrease in appetitive food seeking by measuring preferred meal size in an intra-oral intake task (Supplemental Methods). Appetitive behavior involves a pursuit of reward in the environment and is influenced by the motivational state of the animal (Bindra, 1968; Kelley, 1999), whereas consummatory behavior involves the regulation of intake and is reflected by an animal’s preferred meal size (Foltin and Haney, 2007). Intrategmental CB1 receptor antagonists did not produce changes in cumulative intra-oral intake (Figure 3B right; t(6)=0.3, n.s.) but significantly decreased intake when administered systemically (Figure 3B left; t(6)=−3.4, p<0.01), suggesting that the VTA endocannabinoid system exclusively regulates appetitive aspects of feeding behavior.

Although the doses of rimonabant used in the present study are comparable to those previously shown to reduce the effects of environmental stimuli on motivated behavior without producing nonspecific effects on locomotor activity (Le Foll and Goldberg, 2004), we wanted to further assess whether our reported decreases in reward seeking resulting from CB1 receptor antagonism might be explained by a disruption in either attentional processing or motor performance by assessing the effects of rimonabant on behavior maintained in the 5-choice serial reaction time task. Rimonabant (0.3 mg/kg i.v.) failed to disrupt visuospatial attention, as assessed by accurate choice (Supplemental Figure 2B), or motor performance, as measured by the latency to respond to visual stimuli (Supplemental Figure 2C). These data support that the rimonabant-induced decreases presented herein are due to a specific effect on reward seeking rather than nonspecific behavioral effects on attention or operant performance.

Interval timing, dopamine release and endocannabinoids

In confirmation of our previous report (Cheer et al., 2007a), we observed increases in dopamine concentration preceding cue presentation (Figures 1B, ,2C,2C, 3D, 3H). These data support the theory that dopamine might function to encode information related to interval timing, defined as the duration of time required to organize a behavioral response, under conditions in which reward availability is temporally predictable (Buhusi and Meck, 2005; Matell et al., 2003; Meck, 1996). To directly test this theory, we compared changes in dopamine concentration during ICSS conditions in which cue-presentation was predictable (fixed time out=10-s; FTO) versus conditions in which cue-presentation occurred variably (variable time out; [x with macron] =30-s; VTO). As occurred in the ICSS-FTO task, reward availability was signaled to the animal by the presentation of a compound cue. This signaled reward availability across multiple sensory modalities; specifically, a house light turned off, an ongoing tone ceased and a white stimulus light mounted above the lever was presented. All stimuli were presented simultaneously with lever extension. As predicted, anticipatory dopamine (Figure 4A) was only observed under FTO conditions. Importantly also, the concentration of cue-evoked dopamine was significantly lower under VTO conditions (Figure 4C; MWU test, U=27.5, p=0.032; n=11), which likely reflects a decrease in value imposed by the longer, unpredictable delays in reward availability imposed by the ICSS-VTO task (Bromberg-Martin and Hikosaka, 2011; Day et al., 2010; Kobayashi and Schultz, 2008), while response latencies were significantly increased (Figure 4B; MWU test, U=24, p<0.01; n=14) due to greater operandum disengagement.

Figure 4
Disrupting endocannabinoid signaling decreases reward seeking under experimental conditions that do not recruit neural mechanisms of interval timing

The data presented in Figure 2 demonstrate that rimonabant decreased cue-evoked dopamine signaling and reward seeking in the ICSS-FTO task. Under these conditions however, rather than decreasing reward-directed behavior by interfering with the neural representation of an environmental cue, disrupting endocannabinoid neurotransmission might decrease reward-directed behavior by interfering with an interoceptive timing signal because pharmacological manipulation of either the endocannabinoid or mesolimbic dopamine system can modulate neural representations of time during behavioral tasks (Crystal et al., 2003; Meck, 1983, 1996; Taylor et al., 2007). To address this, we tested the effects of rimonabant using the ICSS-VTO procedure. Rimonabant significantly increased the latency to respond in the ICSS-VTO task (Figure 4D; MWU test, U=0, p<0.05; n=4) as occurred in the ICSS-FTO task, thereby supporting our hypothesis that endocannabinoids regulate reward directed behavior by modulating the encoding of environmental cues predicting reward availability rather than interfering with interval timing.

VDM11 decreases cue-evoked dopamine concentrations and reward seeking

We next sought to assess the effects of augmenting endocannabinoid levels on the neural mechanisms of reward seeking. The ICSS-VTO task was selected to eliminate potential floor effects involving response latency (as latencies to respond in the ICSS-FTO task can be in the subsecond range for well-trained animals). To increase endocannabinoid concentrations, animals were treated with the putative endocannabinoid uptake inhibitor VDM11 using a cumulative dosing approach. Contrary to our hypotheses, VDM11 dose-dependently (300–560 µg/kg i.v.) increased response latency (Figure 5A; F(2,23)=5.69, p<0.01; 560 µg/kg versus vehicle, p=0.013; mean values: b=1.25, v=1.26, 0.1=1.28, 0.3=4.47, 0.56=5.16-s) while decreasing the concentration of cue-evoked dopamine release in a manner similar to rimonabant (Figure 5B; F(4,29)=3.66, p=0.018; 560 µg/kg versus vehicle, p=0.047; also see Supplemental Figure 3A for mean dopamine concentration traces). Figure 5C shows representative color plots and dopamine concentration traces illustrating the effects of vehicle (top) and VDM11 (bottom) in individual trials. These findings suggest that, under these conditions, VDM11 impairs the neural mechanisms of reward seeking by functioning as an indirect CB1 receptor antagonist.

Figure 5
The putative endocannabinoid uptake inhibitor VDM11 decreases dopamine mechanisms of reward seeking in a manner that is consistent with indirect CB1 receptor antagonism

Repeated vehicle treatments fail to affect cue-evoked dopamine concentrations and reward seeking

In addition to observing drug-induced decreases in cue-evoked dopamine concentration however, we noted that the concentration of electrically-evoked dopamine also decreased across trials (Supplemental Figure 1A for Rimonabant; Supplemental Figure 3A for VDM11). This observation led us to test whether the decreases in cue- and electrically-evoked dopamine concentration were drug-induced, or rather, the result of repeated vehicle injections occurring in prolonged ICSS sessions. To address this, we measured changes in NAc dopamine concentration and response latency for brain stimulation reward in the ICSS-VTO task while administering vehicle every 30 responses. Prior to ICSS-VTO session onset, animals were first trained to criterion in the ICSS-FTO task to mimic experimental conditions. Thus, rather than assessing dopamine release events during acquisition (Figure 1), this experiment assessed dopamine concentrations over time as would occur during pharmacological experiments. Best-fit functions revealed that across trials cue-evoked dopamine concentrations quickly increased to an unvarying maximal level (Figure 6A; Exponential Rise to Maximum, Single, 2-Parameter; R2=0.35; F(1,19)=9.85, p<0.01), while response latencies quickly decreased to an unvarying minimal level (Figure 6B; Polynomial, Inverse Second Order; R2=0.25; F(2,39)=6.08, p<0.01). After the first 30 responses, both the concentration of cue-evoked dopamine and response latency remained statistically indistinguishable across binned responses. By contrast, electrically-evoked dopamine concentrations showed greater variability and decreased linearly across trials (Figure 6A; Polynomial, Linear; R2=0.31; F(1,19)=7.90, p<0.01). Representative mean color plots and accompanying dopamine concentration traces (Figure 6C) show dopamine concentrations changing across binned-responses. Identical trends were observed in untreated animals (data not shown). These observations are in agreement with previous reports (Garris et al., 1999; Nicolaysen et al., 1988; Owesson-White et al., 2008) that electrically-evoked dopamine concentrations, but not cue-evoked dopamine concentrations or response strength, decrease during ICSS sessions – an effect that has been attributed to the depletion of a readily releasable pool of dopamine by electrical stimulation (Nicolaysen et al., 1988; Owesson-White et al., 2008; Yavich and Tiihonen, 2000). Moreover, these data demonstrate that repeated vehicle injections fail to affect either cue-evoked dopamine concentrations or response latency. These findings, however, do not completely disprove that the endocannabinoid system might modulate electrically-evoked dopamine release. The variables (e.g., route of administration, pharmacological target) that might influence the actions of endocannabinoids on electrically-evoked dopamine release should be further addressed.

Figure 6
Electrically-evoked, but not cue-evoked, dopamine concentrations are partially depleted during ICSS sessions

2AG but not anandamide facilitates reward seeking

The VDM11 findings prompted us to investigate the specific effects of the endocannabinoids 2AG and anandamide on reward seeking. 2AG and anandamide levels are tightly regulated through distinct enzymatic degradation systems. 2AG is hydrolyzed by the enzyme monoacylglycerol lipase (MAGL), whereas anandamide is hydrolyzed by the enzyme fatty acid amide hydrolase (FAAH) (Cravatt et al., 1996; Long et al., 2009). Recent advances in pharmacology have led to the development of drugs that selectively inhibit either MAGL (JZL184; (Long et al., 2009)) or FAAH (URB597; ((Cravatt et al., 1996; Fegley et al., 2005)), thereby producing specific increases in 2AG or anandamide tissue levels respectively. We began testing the effects of these drugs in mice because JZL184 is known to exhibit reduced potency against MAGL in rats (Long et al., 2009). In mice, JZL184 (Figure 7A; F(2,14)=6.61 p=0.019; 40 mg/kg versus vehicle, p=0.029), but not URB597 (data not shown), increased break points (a metric of motivation) for food reinforcement maintained under a progressive ratio schedule (Supplemental Methods). Importantly, the JZL184-induced increase in break points was prevented by pre-treating mice with a subthreshold dose of AM251 (0.75 mg/kg i.p.), which demonstrates that the JZL184-induced increase in motivation occurred in a CB1 receptor dependent manner. In rats, we observed increased break points (Figure 7A MWU test, U=50.5, p=0.026; n=14) for food reinforcement only after altering the route of administration and unit-injection dose (10 mg/kg JZL184 i.v.). Using a cumulative dosing approach, JZL184 (3–10 mg/kg i.v.) also facilitated reward seeking as assessed by decreased response latency in the ICSS-VTO task (Figure 7B; F(3,15)=4.86 p<0.01; 10 mg/kg versus vehicle, p=0.027; mean values: b=4.02, v=3.93, 3=3.83, 5.6=3.62, 10=4.32-s). By contrast, URB597 treatment (10–56 µg/kg i.v.) was ineffective at altering response latency (Figure 7C; mean values: b=4.25, v=4.19, 10=4.19, 31=6.15, 56=5.63-s) in the ICSS-VTO procedure, or break points for food reinforcement maintained under a progressive ratio schedule (Figure 7A). VDM11 (5.6 mg/kg i.v.) also failed to affect break points for food reinforcement (data not shown). To verify that JZL184 was indeed increasing activation of CB1 receptors, we treated rats with cumulative doses of JZL184 (5.6–10 mg/kg i.v.) while monitoring core body temperature as cannabinoids produce hypothermia in rats (Garattini, 1965; Miras, 1965). As predicted (Long et al., 2009), JZL184 decreased core temperature across time (Figure 7D; F(3,34)=2.63, p<0.01). To definitively test whether JZL184 increases 2AG levels during reward seeking, we assessed lipid content in VTA tissue from JZL184 and vehicle-treated rats upon completion of the ICSS-VTO task and found that JZL184 significantly increased 2AG VTA tissue content in comparison to vehicle (Figure 7E; t(27)=2.07, p=0.048), thereby confirming that JZL184 augments 2AG levels in the VTA during reward directed behavior in the rat.

Figure 7
The endocannabinoid 2AG, but not anandamide, facilitates reward seeking

Increasing 2AG levels facilitates cue-evoked dopamine release and reward seeking

To assess the effects of increasing 2AG levels on the neural mechanisms of reward seeking we treated rats with JZL184 (10 mg/kg i.v.) while responding was maintained by brain stimulation reward in the ICSS-VTO task. As observed using a cumulative dosing approach, JZL184 (10mg/kg i.v.) decreased response latency (Figure 8A; t(14)=2.36, p=0.033; mean values: b=3.55, v=3.48, JZL=2.89-s). Enhanced reward seeking occurred in parallel with an increase in cue-evoked dopamine concentration (Figure 8B; F(2,14)=10.86 p<0.01; 10 mg/kg versus vehicle, p<0.01; also see Supplemental Figure 3B for mean dopamine concentration traces). The effect of JZL184 on dopamine signaling during individual trials is illustrated by the representative color plots and accompanying dopamine concentration traces (Figure 8C), while the effect of JZL184 on dopamine signaling across trials is shown by the representative surface plot (Figure 8D). To confirm that 2AG levels within the VTA are alone sufficient to facilitate the neural mechanisms of reward seeking, we infused JZL184 into the VTA while measuring dopamine concentrations and behavior maintained in the ICSS-VTO task. Although the required vehicle to achieve solubility [a 6µg/0.5µl solution required 100% dimethyl sulfoxide (DMSO)] increased response latency; remarkably, intrategmental JZL184 (6 µg, ipsilateral) reversed the DMSO-induced deficits in reward seeking (Figure 8E; t(6)=−2.51, p=0.046; mean values: b=3.75, DMSO=4.61, JZL=3.47-s) while increasing cue-evoked dopamine concentrations (Figures 8F; F(2,18)=10.84 p<0.01; 6 µg versus vehicle, p=0.023). To verify that the effects of intrategmental JZL184 on reward seeking were CB1 receptor dependent, we then treated rats with a subthreshold dose of rimonabant (1.25 mg/kg i.v.), which reverted response latencies to DMSO conditions. The effects of intrategmental DMSO and JZL184 on cue evoked dopamine events occurring in individual trials are illustrated by the representative traces in Figure 8G, whereas the effects across trials are depicted in a representative surface plot (Supplemental Figure 4E). JZL184-induced increases in cue-evoked dopamine concentration and reward seeking can also be observed by viewing audio-visual material (Supplemental Video 3). Taken together, these data suggest that augmenting 2AG within the VTA is sufficient to facilitate mesolimbic dopaminergic mechanisms of reward seeking.

Figure 8
The endocannabinoid 2AG facilitates dopaminergic mechanisms of reward seeking

DISCUSSION

It is well documented that transient dopamine concentrations in the NAc encode information regarding motivationally salient stimuli that predict reward availability (Day et al., 2007; Flagel et al., 2011; Phillips et al., 2003). Little is known however, regarding how these transient increases are modulated at dopamine cell bodies within the VTA. In the present study, we used a cutting-edge electrochemical monitoring technique to investigate how endocannabinoids in the VTA modulate transient dopamine release into the NAc shell during reward seeking. We found that disrupting endocannabinoid modulation of dopamine neurons reduced cue-evoked dopamine concentrations and reward seeking. Moreover, we identified that 2AG, rather than anandamide, is the primary endocannabinoid responsible for facilitating the neural mechanisms of reward seeking. Thus, our findings reveal that the VTA endocannabinoid system is critical for the fine-tuned regulation of dopamine signaling that mediates reward-directed behavior.

Our data demonstrate the existence of a single neural signaling mechanism through which CB1 antagonists can effectively diminish the influence that environmental cues exert over motivated behavior. A number of studies have shown that the endocannabinoid system is involved in the appetitive-motivational aspects of reward-directed behavior. For example, motivation for both palatable foods (Ward and Dykstra, 2005) and drugs of abuse (Solinas et al., 2003; Xi et al., 2007) is decreased by pharmacological disruption of endocannabinoid signaling as assessed by break points under a progressive ratio schedule. A current theory holds that endocannabinoids are specifically involved in modulating the secondary/environmental influences on motivated behavior (Le Foll and Goldberg, 2004; Vries and Schoffelmeer, 2005). In support of this, when responding is maintained by conditioned cues (i.e. under a second order schedule), pharmacological disruption of endocannabinoid signaling decreases responding (Justinova et al., 2008). Moreover, endocannabinoid disruption is particularly effective at reducing cue-induced reinstatement, a model of relapse in humans that incorporates the influence of conditioned environmental stimuli on reward seeking (Epstein et al., 2006). In this model, CB1 receptor antagonists decrease the propensity for conditioned cues to reinstate responding for appetitive food (Ward et al., 2007) and various drugs of abuse (Justinova et al., 2008; Vries and Schoffelmeer, 2005). Importantly, the finding that disrupting endocannabinoid signaling decreases reward seeking regardless of the reinforcer paired with the cue (Vries and Schoffelmeer, 2005) implies that a common neural mechanism is involved through which endocannabinoids regulate cue-motivated behavior. Our data suggest that this common neural mechanism involves endocannabinoid disinhibition of cue-evoked dopamine cell firing in the VTA, as pharmacological disruption of endocannabinoid signaling within this brain region was sufficient to decrease cue-evoked dopamine concentrations and reward seeking behavior in unison. It is likely that following systemic administration of CB1 receptor antagonists; however, diminished surges in dopamine concentration interact with altered accumbal glutamate concentrations (Xi et al., 2007), possibly arising from the prefrontal cortex (Alvarez-Jaimes et al., 2008), to decrease reward seeking. Such an interaction would be consistent with the theory that accumbal dopamine affects reward seeking by modulating convergent cortical, hippocampal and amygdalar input (Brady and O'Donnell, 2004; Floresco et al., 2001). Furthermore, CB1 receptors within the NAc likely contribute to decreased reward seeking following systemic administration of CB1 receptor antagonists (Alvarez-Jaimes et al., 2008). Nevertheless, our findings that intrategmental disruption of endocannabinoid signaling alone simultaneously decreased cue-evoked dopamine concentrations and reward seeking suggests that the VTA endocannabinoid system is critically involved in mediating cue-motivated reward directed behavior.

We therefore predicted that increasing endocannabinoid levels would facilitate the neural mechanisms of reward seeking. VDM11 however, dose-dependently decreased cue-evoked dopamine signaling and reward seeking in a manner that is more consistent with VDM11 reducing presynaptic CB1 receptor activation. These findings are in agreement with recent reports demonstrating that endocannabinoid uptake inhibitors can decrease cue-induced reinstatement of drug-seeking behavior in a manner similar to rimonabant when assessed using self-administration (Gamaleddin et al., 2011) or conditioned place preference paradigms (Scherma et al., 2011). One possible mechanism explaining these findings is that VDM11 decreases CB1 receptor activation by interfering with the bi-directional release of endocannabinoids through a putative transport mechanism (Hillard et al., 1997; Melis et al., 2004; Ronesi et al., 2004). Another mechanistic explanation is that VDM11 might selectively increase anandamide (Van der Stelt et al 2006), which could function as a competitive antagonist at CB1 receptors in the presence of 2AG because, in contrast to 2AG, anandamide is a partial agonist at CB1 receptors (Howlett and Mukhopadhyay, 2000). These findings led us to investigate the respective contributions of 2AG and anandamide. 2AG, but not anandamide, increased motivation, reward seeking and cue-evoked dopamine concentrations. These data demonstrate that 2AG is the primary endocannabinoid that enhances the neural mechanisms of cue-motivated reward seeking and agree with reports demonstrating that 2AG is the principal endocannabinoid for multiple forms of synaptic plasticity across several brain regions (Melis et al., 2004; Tanimura et al., 2010).

Based on our data, we speculate that 2AG might modulate cue-evoked dopamine release through disinhibition of dopamine neurons in the VTA. When dopamine neurons fire at high frequencies they release 2AG (Melis et al, 2004), which then retrogradely binds to CB1 receptors on presynaptic terminals within the VTA (Lupica and Riegel, 2005). Although 2AG would affect both GABAergic and glutamatergic synaptic input through CB1 receptor activation (Matyas et al., 2008) – cue-encoding VTA dopamine neurons are theorized to form discrete neural assemblies with GABAergic synapses, thereby allowing for the fine-tuned regulation of dopamine neural activity during reward seeking (Lupica and Riegel, 2005; Matyas et al., 2008). According to this conceptualization, 2AG activation of CB1 receptors located on GABAergic terminals might decrease GABA release onto VTA dopamine neurons. The reduced GABA tone theoretically would decrease activation of GABA receptors on VTA dopamine neurons, thus resulting in a disinhibition of dopamine neural activity (Lupica and Riegel, 2005). The resulting disinhibition of dopamine neural activity is theorized to facilitate the neural mechanisms of reward seeking. It is important to clarify that using this freely moving recording approach, other mechanisms within the VTA may account for the observed findings.

We further speculate that endocannabinoid modulation of dopamine release from the VTA might affect NAc neural activity through a D1 receptor dependent mechanism. While recent evidence indicates that dopamine does not directly change postsynaptic excitability in the NAc (Stuber et al., 2010; Tecuapetla et al., 2010), it remains well accepted that dopamine can modulate input into the striatum, as occurs during reward seeking, to affect neural responses in a D1 receptor dependent manner (Cheer et al., 2007a; Goto and Grace, 2005; Reynolds et al., 2001). It is possible therefore, that the VTA endocannabinoid system might affect NAc neural activity by increasing D1 receptor occupancy. Recently developed computational models of dopamine signaling offer insight into how dopamine transients might influence NAc neural activity specifically through a D1 receptor-mediated mechanism (Dreyer et al., 2010). When dopamine neurons exhibit regular pacemaker firing, low concentrations (i.e. tonic) of dopamine are released throughout the NAc (Floresco et al., 2003). The computational model predicts that during tonic dopamine signaling, D2 receptors approach maximal occupancy whereas D1 receptors remain relatively unaffected (Dreyer et al., 2010). By contrast, when dopamine neurons fire at high frequency, transient bursts of dopamine are heterogeneously released into discrete microcircuits of the NAc (Dreyer et al., 2010; Wightman et al., 2007). When these higher concentration transients occur – D1 receptor occupancy theoretically increases precipitously whereas D2 receptors, which are already approaching maximal occupancy, remain relatively unaffected (Dreyer et al., 2010). Thus, we hypothesize that endocannabinoid disruption in the VTA might decrease NAc neural activity by preventing sufficient D1 receptor occupancy.

The present study offers previously unseen insights regarding the neural mechanisms underlying reward seeking motivated by conditioned cues. Our data demonstrate for the first time that 2AG within the VTA can modulate cue-evoked dopamine transients, which are theorized to promote reward seeking (Nicola, 2010; Phillips et al., 2003). While we (Cheer et al., 2007b) and others (Perra et al., 2005) have demonstrated that disrupting the VTA endocannabinoid system decreases drug-induced dopamine release, this is the first demonstration that the endocannabinoid system modulates cue-evoked dopamine transients during the pursuit of reward. Furthermore, our data suggest that drugs designed to specifically manipulate 2AG levels may prove to be effective pharmacotherapies for the treatment of neuropsychiatric disorders involving a maladaptive motivational state.

EXPERIMENTAL PROCEDURES

Subjects

Male Sprague-Dawley rats, ~90–120 days old (300–350g), fitted with back mounted jugular vein catheters at vendor (Charles River) were used as subjects. Subjects were anesthetized with isoflurane (5% isoflurane induction, 2% maintenance) in a Kopf stereotaxic apparatus and implanted with a microdialysis guide cannula (BAS) aimed at the NAc shell (+1.7 AP, +0.8 ML), an ipsilateral bipolar stimulating electrode (Plastics One) in the VTA (−5.4 AP, +0.5 ML, −8.7 DV), and a contralateral Ag/AgCl reference electrode. All procedures were performed in accordance to the University of Maryland, Baltimore’s Institutional Animal Care and Use Committee protocols.

Fast-scan cyclic voltammetry

Dopamine was detected from fast-scan cyclic voltammograms collected at the carbon fiber electrode every 100ms (initial waveform: −0.4V to 1.3V, 400V/s (Heien et al., 2003)). Principal component regression (PCR) was used as previously described to extract the dopamine component from the raw voltammetric data (Heien et al., 2005).

Dopamine signal calibration

Principal component regression (PCR) was used as previously described to extract the dopamine component from the raw voltammetric data (Heien et al., 2005). A calibration set of stimulations was obtained for each experiment varying number of stimulation pulses (6, 12, or 24) and frequency (30 or 60 Hz). Scaling factors for both DA and pH were obtained post experiment by placing the electrode into a flow injection system and injecting known concentrations of DA and pH into artificial cerebro-spinal fluid. These scaling factors related current values to concentration values.

Microinfusions

For experiments involving intrategmental infusions, rats were unilaterally treated with vehicle (DMSO; 0.5µl), rimonabant 200ng/0.5µl or JZL184 6µg/0.5µl. Infusions occurred in the experimental chamber using a microprocessor-controlled infusion pump (Harvard Apparatus). An infusion needle was inserted through a guide cannula ending 1mm above the tip of a bilateral stimulating electrode (Plastics One); the needle was cut to extend 1mm beyond the cannula tip.

Histology

Rats were placed under deep anesthesia (2mg/kg urethane). A high amplitude current (500 µA) was applied through a stainless steel electrode to verify working electrode placement. Rats were then intracardially perfused with saline, potassium ferrocyanide stain, and 10% formalin. Brains were removed, cryoprotected and coronally sectioned using a cryostat. See Supplemental Figure 5 for representative illustrations confirming electrode placement.

Statistics

Behavioral analyses were statistically evaluated using the Shapiro-Wilk test for normality. If not normally distributed, data were analyzed with either the Mann-Whitney U (MWU) test or Kruskal-Wallis ANOVA on ranks. If normally distributed, data were analyzed with either the Student’s t-test or ANOVA. Dopamine concentrations occurring during the first second of cue presentation were analyzed with ANOVA and Bonferroni post-hoc tests. All statistical analyses were performed with SigmaPlot (version 11).

Highlights

  • VTA endocannabinoid tone regulates neural mechanisms of cue-motivated reward seeking
  • CB1 receptor antagonists decrease the neural mechanisms of reward seeking
  • VTA levels of 2AG not anandamide facilitate the neural mechanisms of reward seeking
  • Drugs targeting these systems might successfully be used in disorders of motivation

Supplementary Material

01

02

Acknowledgments

Funding for this study was provided by NIH grants R01DA022340 (JFC), R01DA025634 (MFR), P01DA009789 (AHL), F32DA032266 and T32NS007375 (EBO), and a Rubicon Fellowship (CSL). We also thank Geoff Schoenbaum, Peter Shizgal, Yolanda Mateo and Joshua Jones for helpful comments in the preparation of this manuscript, John Peterson for instrumentation assistance and Merce Masana, Niels Vos, Ronny Gentry and David Bernstein for technical assistance.

Footnotes

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