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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Cell. Author manuscript; available in PMC Jan 20, 2013.
Published in final edited form as:
PMCID: PMC3268671
NIHMSID: NIHMS345845
Endoplasmic Reticulum PI(3)P lipid binding targets malaria proteins to the host cell
Souvik Bhattacharjee,1 Robert V. Stahelin,2 Kaye D. Speicher,3 David W. Speicher,3 and Kasturi Haldar1*
1Center for Rare and Neglected Diseases, University of Notre Dame, 103 Galvin Life Sciences, Notre Dame, IN 46556, USA
2Department of Biochemistry & Molecular Biology, Indiana University School of Medicine-South Bend, 143 Raclin-Carmichael Hall, 1234 Notre Dame Avenue, South Bend, IN 46617, and Department of Chemistry & Biochemistry University of Notre Dame, Notre Dame, IN 46556, USA
3Center for Systems and Computational Biology and Molecular and Cellular Oncogenesis Program (Room 151), Wistar Proteomics Core Facility (Room 154), The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
*Corresponding author Contact information: khaldar/at/nd.edu
Hundreds of effector proteins of the human malaria parasite Plasmodium falciparum constitute a `secretome', carrying a host-targeting (HT) signal, which predicts their export from the intracellular pathogen into the surrounding erythrocyte. Cleavage of the HT signal by a parasite endoplasmic reticulum (ER) protease, plasmepsin V, is the proposed export mechanism. Here we show that the HT signal exports by recognition of the lipid phosphatidylinositol-3-phosphate (PI(3)P) in the ER, prior to and independent of protease action. Secretome HT signals, including those of major virulence determinants bind PI(3)P with nanomolar affinity and amino acid specificities displayed by HT-mediated export. PI(3)P-enriched regions are detected within the parasite's ER, co-localize with endogenous HT signal on ER precursors, which also display high affinity binding to PI(3)P. A related, pathogenic oomycete's HT signal export is dependent on PI(3)P binding, without cleavage by plasmepsin V. Thus PI(3)P in the ER functions in mechanisms of secretion and pathogenesis.
Keywords: Plasmodium falciparum, pathogenic protein export, secretory PI(3)P regions
Malaria continues to extract a major toll on human health. The most virulent of human malarias is caused by the protozoan parasite Plasmodium falciparum. It is responsible for a million deaths in children each year, largely in sub-Saharan Africa (WHO, 2010). The symptoms and pathologies of disease are caused by blood stage parasites that infect erythrocytes (Miller et al., 2002). The intra-erythrocytic parasite resides and proliferates within a parasitophorous vacuolar membrane (PVM; Figure 1). Proteins are exported from the parasite, across the PVM into the cytoplasm and membrane of the erythrocyte to induce changes in transport and membrane properties of the host cell (Haldar and Mohandas, 2007; Maier et al., 2009; Nguitragool et al., 2011). Several hundred P. falciparum proteins are predicted to be released into the erythrocyte (Hiller et al., 2004; Marti et al., 2004; Sargeant et al., 2006; van Ooij et al., 2008) including adhesins directly linked to severe and fatal disease pathologies of cerebral malaria and placental malaria (Kyes et al., 2007; Duffy and Fried, 2003).
Figure 1
Figure 1
Schematic of intracellular infection of Plasmodium and targeting parasite proteins to the host erythrocyte
Parasite proteins destined for the erythrocyte are expected to be first recruited into the endoplasmic reticulum (ER) via an N-terminal signal sequence or a transmembrane domain (Lopez-Estrano et al., 2003). The presence of a consensus host (cell) targeting (HT) or PEXEL (Plasmodium Export Element) signal RxLxE/D/Q downstream of the signal peptide or transmembrane domain, is known to export proteins to the erythrocyte (Hiller et al., 2004; Marti et al., 2004; Sargeant et al., 2006; van Ooij et al., 2008). As shown schematically in Figure 1, the HT signal is cleaved in the parasite's ER (Chang et al., 2008; Osborne et al., 2010). In vitro data suggest that cleavage is due to a resident ER protease plasmepsin V (Boddey et al., 2010; Russo et al., 2010) that functions in lieu of signal peptidase to release newly synthesized protein from the ER membrane. Cleavage by plasmepsin V is also proposed to be the mechanism for host targeting (Boddey et al., 2010; Russo et al., 2010), but the underlying mechanisms remain unknown.
The malarial HT signal is related both in sequence and function to the host-targeting signal of another eukaryotic pathogen, the oomycete Phytophthora infestans, which infects plant cells and caused the Irish potato famine (Whisson et al., 2007). The P. infestans signal is composed of the sequence RxLR…DEER, which if expressed in P. falciparum, can target reporter proteins to the host erythrocyte (Bhattacharjee et al., 2006). Conversely malarial HT signal when expressed in P. infestans catalyzes protein export to the host plant cell (Dou et al., 2008). In addition, the oomycete signal RxLR…DEER has recently been shown to bind PI(3)P on the surface of eukaryotic cells as a means of penetrating the host (Kale et al., 2010) (Figure S1). The binding of this HT signal to PI(3)P was a surprising finding. Polyphosphoinositide binding and binding to the phosphomonoester phosphatidic acid have been attributed to small but cationic rich motifs (McLaughlin et al., 2002; Stace and Ktistakis, 2006). However, to date identified PI(3)P effectors have a well-defined PI(3)P-binding pocket in FYVE or PX domains and generally have at least four points of contact with PI(3)P (Kutateladze, 2010). Although the initial specificity of HT signal binding to PI(3)P was established, the underlying mechanism of binding remains undefined. The significance of HT signal binding to lipid for malarial parasites is unknown, because malarial HT signals are cleaved in the ER and no cell surface PI(3)P was detected on host erythrocytes (Kale et al., 2010). Thus malarial effector proteins cannot utilize PI(3)P to translocate the PVM into the erythrocyte (Figure 1).
PI(3)P binds the malarial HT with both affinity and specificity linked to export and is detected in the parasite's ER
To investigate the phosphoinositide (PI) specificity of the HT signal (RLLYE) of P. falciparum histidine-rich protein II (PfHRPII), green and red fluorescent protein (GFP and RFP) fusions were used in lipid sedimentation assays (Figures 2A, S2 and Table S2). Clearly, both constructs displayed high selectivity for PI(3)P containing vesicles, while mutation of the consensus sequence (ALAYA) or the introduction of a scrambled (YE) sequence significantly reduced PI(3)P binding. To further demonstrate the specific nature of PI(3)P binding, Ins1,3P2 was added to the lipid sedimentation assay to compete with PI(3)P vesicles for HT binding. In both instances (HT-GFP and HT-RFP), Ins1,3P2 greatly reduced the amount of HT bound to PI(3)P containing vesicles (Figure S2D). Additionally, HT-GFP was able to compete with the high affinity PI(3)P binding PX domain of p40-phox and displace p40-phox-PX from PI(3)P vesicles in a concentration dependent manner (Figure 2B).
Figure 2
Figure 2
Lipid Binding Properties of HT Signals
A lack of quantitative information on the affinity of PI(3)P binding by HT-targeting signals of oomycete/malaria, precludes understanding their relative mechanism of action. We therefore used surface plasmon resonance (SPR) and as shown in Figure 2C-E, discovered a high affinity interaction between the HT signal of PfHRPII and PI(3)P vesicles (36 nM). This was completely abolished by mutation ALAYA (Kd > 5 μM). The mutation ALLYE (Kd > 5 μM) also abrogated PI(3)P binding, while RLAYE and RLLYA reduced binding by 23- and 7-fold, respectively. The relative contribution of R, L and E residues in the HT signal to PI(3)P binding correlated with their relative utilization in the host targeting logo (Figure 2E), suggesting lipid binding and protein export may be linked. Additional HT signals from the major virulence determinant P. falciparum erythrocyte membrane protein 1 (PfEMP1) and other parasite effectors known to be targeted into the host erythrocyte, also bound PI(3)P vesicles with nanomolar affinity ranging from 20 to 110 nM. Mutation of R/K with alanine decreased binding by at least 20-fold (Figure 2E) and, in most cases, binding to PI(3)P was not detectable, suggesting that HT signals of multiple, important malarial effector proteins bind PI(3)P. Together these data led us to consider that PI(3)P binding may be a generalized property of a wide range of malaria secretome effectors exported to the erythrocyte.
However, PI(3)P is known to be a cytoplasmic lipid in cells. To bind the HT signal on newly synthesized parasite effectors exported to the erythrocyte, it must be present in the lumen of parasite's secretory pathway and act prior to processing by plasmepsin V in the ER. To the best of our knowledge, there are no reports of the presence of PI(3)P within the secretory pathway of cells. Thus, to investigate this possibility in the malarial secretory pathway, we expressed a secretory fusion of monomeric Cherry (mCherry) and the FYVE domain of early endosomal antigen 1 (EEA1), a protein with nanomolar affinity for PI(3)P (and is widely used to detect the location of PI(3)P in cells (Lee et al., 2005). As shown in Figure 3A, all of the cell associated red fluorescence is detected in punctate regions in a perinuclear location (upper panel), ascribed to a single fusion protein of the expected size (Figure 3B) and recruited to the secretory pathway (as proven by cleavage of the signal sequence, Figure 3C). A point mutation R1374A in the FYVE region of EEA1, known to abrogate PI(3)P binding when introduced into the mCherry fusion, redistributed red fluorescence to the parasite periphery (Figure 3A, lower panel), and was released into the supernatant when the red cell and PVM were permeabilized with saponin (Figure 3D), consistent with default secretion into the parasitophorous vacuole (PV). A secretory fusion of a second PI(3)P binding protein (p40-phox-PX) also showed perinuclear, punctate distribution (Figure S3A), while a single point mutant p40-phoxR58Q that abrogates PI(3)P binding, is secreted to the PV (Figures S3A and S3B); again suggesting that PI(3)P is present in highly localized secretory regions within the parasite. These PI(3)P enriched regions appear to reside in a subset of structures labeled by BiP, a resident ER protein (Figures 3E and S3C) and also show overlap with plasmepsin V (another parasite ER protein) but their distribution appears to be distinct from that of the early Golgi marker (ERD2) and a protein of the PVM surrounding the intracellular parasite (Figure 3E). This suggested that PI(3)P-enriched regions are likely to be present early in the secretory pathway, in the ER, but are not concentrated in the Golgi or periphery of the parasite. Immunoelectron microscopy studies revealed that PI(3)P is detected in regions of reticular membranes closely apposed to the nucleus (Figures 3F and S3D), and localizes to perinuclear, tubovesicular structures containing the ER marker, BiP, confirming its localization in the ER (Figure 3G).
Figure 3
Figure 3
PI(3)P is detected in P. falciparum endoplasmic reticulum
An endogenous, ER form of PfHRPII protein containing the HT signal recognizes PI(3)P prior to cleavage by plasmepsin V
Since PI(3)P is found in the malarial ER and displays nanomolar affinity for HT signal in in vitro assays, we investigated whether the HT signal on the endogenous ER form of an effector protein is associated with PI(3)P. Since plasmepsin V cleaves to separate the sequences upstream of the HT signal from the rest of the effector protein, we generated antibodies to recognize the uncleaved precursor protein form but not the processed mature protein.
As shown in Figures 4A–B, antibodies to the pre-HT region of PfHRPII protein (also called anti-(pre-HT)) recognized recombinant protein produced in E. coli from constructs 1–4, but not 5–6 that lacked pre-HT region. In immunolocalization assays, pre-HT antibodies recognize protein only within the parasite (Figure 4C, upper panel). In contrast, antibodies to sequences (YETQAHVDDVHHAHHADV) downstream of the HT signal, also called post-HT-antibodies, detect PfHRPII exported to the erythrocyte as well as within the parasite (Figure 4C, lower panel). In Western blots, pre-HT antibodies recognized a single, endogenous protein band above the 50-kDa marker (asterisk in Figure 4D, left panel) restricted to the parasite pellet upon saponin treatment. In contrast, post-HT antibodies also recognize a second exported form released into the supernatant (arrowhead in Figure 4D, right panel). These data confirm the presence of a precursor PfHRPII (pPfHRPII) protein containing the HT motif located within the parasite. Further, pPfHRPII is in the ER and serves as its marker, since plasmepsin V (which cleaves the HT motif of pPfHRPII) resides in the ER.
Figure 4
Figure 4
Endogenous HT signal on precursor PfHRPII (pPfHRPII) in the ER associates with PI(3)P both in vitro and in vivo
It should be noted that these experiments were undertaken with parasites ~12 h in the intraerythrocytic infection cycle, when PfHRPII is maximally synthesized, to facilitate detection of endogenous precursors. Endogenous pPfHRPII was immunopurified using pre-HT antibodies, and found to contain ~ ten-fold higher levels of PI(3)P relative to complexes immunopurified with post-HT antibodies, on a per mole protein basis (Figures 4E and S4). The extent of concentration was comparable to the level of PI(3)P binding observed with secretory EEA1WT relative to binding by the EEA1R1374A mutant (Figures 4F and S4). SPR analyses confirmed that the endogenous HT-signal bearing precursor displayed high affinity for PI(3)P (Figure 4G). The interaction of the pPfHRPII with PI(3)P was specific since the interaction with a second phosphoinositide, PI(4)P was undetectable (Figure 4H). Together, these biochemical analyses firmly establish that endogenous HT signal can be detected on protein prior to cleavage by plasmepsin V and thus resident in the ER. This ER precursor binds PI(3)P with nanomolar affinity. Major sites of PI(3)P/EEA1WT accumulation, showed partial co-association with pPfHRPII in indirect immunofluorescence assays (Figure 4I). It should be noted that in cells endogenous pPfHRPII will compete with (potentially) hundreds of additional effectors for PI(3)P in the ER. Nonetheless colocalization of pPfHRPII and EEA1WT was also seen in perinuclear regions by immunoelectron microscopy (Figure 4J), strongly supporting that pPfHRPII and PI(3)P come together in the ER (or ER-derived membranes).
HT-signal-dependent and -independent export of parasite proteins to the host erythrocyte, in absence of cleavage by plasmepsin V
In the parasite ER, PI(3)P binding by the malarial HT signal must precede HT signal cleavage by plasmepsin V. However, since the relative importance of the individual residues in PI(3)P binding are the same as those for protease cleavage, it is difficult to resolve the relative importance of each to host targeting. To do so, we turned to the oomycete HT signal that we have previously shown to mediate protein export from the parasite to the host erythrocyte (Bhattacharjee et al., 2006). Since the susceptibility of the RQLR…DEER motif to the malarial protease was unknown, we engineered a signal peptidase cleavage site by insertion of AAAA, immediately after the signal sequence and prior to the HT motif of the oomycete Phytophthora infestans effector protein Nuk10, fused to GFP (see Extended Experimental Procedures). Expression of this construct in P. falciparum, resulted in export of GFP from the malaria parasite to the erythrocyte (Figure 5A, top panel and Figure 5B), which was blocked by mutation of the RQLR motif (Fig. 5A, bottom panel).
Figure 5
Figure 5
P. infestans Nuk10 HT signal shows PI(3)P binding- dependent export and is not cleaved by plasmepsin V
To investigate the relative contribution of plasmepsin V cleavage to export, we undertook mass spectrometric analysis of purified Nuk10-WT-GFP and mutant. Analysis of the most N-terminal peptide, suggested cleavage at the AAAA site for both (Figures 5C–D), as expected for signal peptidase action. Quantitative analysis of all released peptides confirmed that the efficiency of signal peptide cleavage was comparable for both (Figure 5E and Table S3). Further, intact RQLR containing peptide was recovered from immunopurified Nuk10 protein (Figure 5F). This was observed even with the Nuk10 fraction exported to the erythrocyte (Figures S5A–D). These data establish that both the Nuk10-WT and the mutant are released from the ER membrane by signal peptidase and that the oomycete HT motif was not cleaved, likely because it is not a substrate for malarial plasmepsin V. This was unexpected, since cleavage by plasmepsin V was proposed as the export mechanism, yet the oomycete HT signal appears to catalyze export in absence of cleavage. SPR measurements confirm that Nuk10 HT signal bound PI(3)P containing vesicles with 28 nM affinity, while the mutant greatly reduced PI(3)P binding (Kd > 2 μM, Figures 5G, S5E), suggesting that PI(3)P binding is the mechanism of export to the host cell.
The principal reason that plasmepsin V is thought to target proteins for export, was the evidence that cleavage of a signal anchor by signal peptidase failed to export protein to the erythrocyte. To further investigate the idea, we engineered an efficient signal peptidase cleavage site (AAAA) in two distinct malaria protein chimeras in which the HT motif was abrogated but the flanking sequences were kept intact (Figure 6). For PfHRPII, we found that mutation of HT signal did not block export of the GFP reporter to the erythrocyte (Figure 6A, middle panel). However, replacement of all 9 downstream charged residues blocked export (Figure 6A, bottom panel). Mass spectrometric analysis confirmed all three chimeras were released from the signal anchor either through cleavage of the HT signal or the AAAA signal peptidase site (Figure S6). Hence, the block in export seen in Fig. 6A bottom panel, is not due to failure to release protein from the membrane. These data suggest that there are HT-independent, peptidic signals of parasite protein export to the erythrocyte that may be dependent on charge.
Figure 6
Figure 6
Evidence for HT-independent protein export to the red cell
For a second protein, we examined P. falciparum erythrocyte membrane protein 3 (PfEMP3), utilized in earlier studies implicating plasmepsin V as the export mechanism (Boddey et al., 2010). As previously reported, replacement of RSLAQ motif with A-AQ abrogated protein export (Figure 6B, top and middle panel) and resulted in a shift from cleavage by plasmepsin V to cleavage by signal peptidase (Figure S6). However, we show that insertion of the AAAA signal peptidase site followed by replacement of RSLAQ by ASAAA, results in export of GFP to the erythrocyte (Figure 6B, bottom panel). For this reporter, the primary site for cleavage is within the AAAA sequence, suggesting that it too is recognized by signal peptidase and released from the membrane. Although we do not understand the basis of GFP export in Figures 6A middle panel and 6B lower panel, the data affirm mutations in the HT signal system may shift transport by an HT- independent export pathway. Thus, the earlier conclusion (Boddey et al., 2010) that failure to export in Fig. 6B middle panel, is due to loss of the HT signal alone, may have been premature.
The HT signal functions in context of a ~40 amino acid vacuolar translocation signal needed to mediate parasite protein export to the erythrocyte (Hiller et al., 2004; Lopez-Estrano et al., 2003). Thus the HT signal may be one (likely the most important) region necessary for selective PI(3)P recognition. In malarial HT signals, PI(3)P binding is critically dependent on the high value R in the plasmodial HT logo, providing one of the first clues that lipid binding may play a major role in targeting malaria parasite proteins to the host erythrocyte. The nanomolar affinity displayed by the HT signal of virulence determinants containing the R/KxLxE motif, including the major virulence adhesin PfEMP1, suggests a new mode of PI(3)P binding. Since nanomolar phosphoinositide (PI) binding is primarily attributed to at least four points of contact, it is likely that there are other cationic residues residing in the HT signal and flanking sequences that contribute to PI(3)P recognition. Additionally, hydrophobic residues such as L or others within the HT signal may provide further membrane anchorage through membrane penetration (Lemmon, 2008), while the consensus E could form H-bonds with the 5-OH and discriminate against other PIs as previously reported for the FYVE domain (Dumas et al., 2001). Further structural and biophysical studies will be necessary to fully appreciate this novel mechanism of PI(3)P recognition.
The presence of PI(3)P in the malarial ER and its affinity for endogenous HT signals, strongly suggests that HT-signal lipid interactions occur early in the ER. Importantly, with the development of specific antibody reagents, we are able to detect ER precursors carrying the HT signal, suggesting that even at steady state a detectable amount of endogenous precursor carrying the HT signal has not been processed by plasmepsin V. Our finding that PI(3)P binding is required for export even when plasmepsin V fails to cleave the oomycete HT signal, leads us to propose that the export mechanism is an efficient, early sorting event in the ER (Figure 7) dependent on high affinity binding to PI(3)P in the lumen of the ER. When it occurs, HT signal cleavage by plasmepsin V is expected to be restricted to an emerging and/or possibly sealed vesicle. The convergence of amino acid specificity of PI(3)P binding and plasmepsin V cleavage, may suggest that association by PI(3)P may facilitate protease cleavage by plasmepsin V. However cleavage per se (whether by plasmepsin V or signal peptidase) may not provide specificity for host targeting, rather both release protein from the ER membrane.
Figure 7
Figure 7
A model for PI(3)P dependent export from the ER of P. falciparum-infected erythrocytes
Our data also suggest that HT-independent export of parasite protein reporters to the host can occur. HT-independent pathways of export have been proposed for major parasite proteins such as P. falciparum Skeleton binding protein 1 (PfSBP1) as well as other proteins, which lack the HT signal but which are resident in the secretory structures called Maurer's clefts in the erythrocyte cytoplasm and are thought to promote protein delivery to the host erythrocyte membrane and other destinations in the erythrocyte (Cooke et al., 2006; Spielmann and Gilberger, 2010). Our data suggest that non HT-dependent export to the erythrocyte is based on charge. Chaperones that interact with charged residues may well distinguish pathways of protein export to the erythrocyte relative to default secretion of well folded secretory proteins to the PV but these need not be linked to chaperones reported to be associated with plasmepsin V (Goldberg and Cowman, 2010; Russo et al., 2010).
In summary, the parasite likely sorts newly synthesized proteins into two, possibly three different export pathways that emerge from the ER, thus, separating cargo for the erythrocyte from the PV (Figure 7), akin to early models of secretion proposed over a decade ago (Elmendorf and Haldar, 1993; Wiser et al., 1997). Wiser et al (1997) proposed a 'secondary ER' for protein export to the erythrocyte and this was supported by rapid kinetics of secretory protein exit (Crary and Haldar, 1992), but evidence of specialized ER domains in parasite protein export was lacking. The contribution of the Golgi to the PI(3)P/HT-signal ER exit pathway, remains unknown. A translocon mediating export of secretome proteins into the erythrocyte has been proposed (de Koning-Ward et al., 2009). How it recognizes putative protein cargo is unclear, since the HT signal in most plasmodial proteins is largely abrogated in ER exit.
Based on the steady state distribution of secretory PI(3)P and plasmepsin V, we propose that both recycle back to the ER once HT signal sorting and cleavage are completed (Figure 7), at least in early intraerythrocytic stages called ring and early trophozoite stages investigated in this study. Recent studies have reported the export of a PI3kinase into the erythrocyte and in association with membrane structures in the red cell (Vaid et al., 2010), but how this export occurs and whether PI3kinase is recruited in the secretory pathway is not known. An alternate mechanism for concentration of PI(3)P in the ER, could be trans-bilayer import of PI(3)P synthesized on the cytoplasmic face. A recent study suggested that blood cell infection increases PI(3)P levels (Tawk et al., 2010), although the overall ratio of PI(3)P in the ER to total cellular PI(3)P is unknown.
The presence of PI(3)P in the lumen of ER regions and its binding to HT signals, is consistent with a sorting function for this lipid in malaria parasites. Given that malarial PI(3)P is utilized by the oomycete HT signal to target proteins to the host erythrocyte and malarial signals and oomycete signals are functionally equivalent in both Plasmodium and Phytophthora, it is possible that PI(3)P also functions in the P. infestans ER. Thus PI(3)P binding in the ER may be a generalized mechanism for pathogenic secretion in eukaryotic pathogens, aspects of which may be targeted to disrupt pathogen-host interactions that underlie disease.
Plasmid construction
All the primers used for vector construction are described in the Extended Experimental Procedures and Table S1.
Expression and purification of recombinant proteins in E. coli
All recombinant proteins were expressed in E. coli BL21 (DE3) cells. His-tagged proteins were purified over Pro-Bond resin (Invitrogen), MBP-tagged proteins were purified over Amylose resin (New England Biolabs) and GST-fusions were purified over Glutathione resin (Clontech). The sequences of recombinant proteins are shown in Table S2. See Extended Experimental Procedures for additional details.
Generation of anti-peptide antibodies
We used peptides NNLCSKNAKGLNLNKRLL (pre-HT) and YETQAHVDDVHHAHHADV (post-HT), based on sequence N-terminal or C-terminal, respectively to the deduced plasmepsin V cleavage site of PfHRPII. Rabbit polyclonal antibodies raised to each were designated as anti-(pre-HT) and anti-(post-HT), respectively.
Parasite culture and live cell imaging
P. falciparum 3D7 parasites and transgenic parasites were propagated in culture in A+ human erythrocytes in RPMI containing Albumax II (Gibco) and were imaged live in a biological chamber using DeltaVision Deconvolution microscopy (Applied Precision). See Extended Experimental Procedures for additional details.
Immunoprecipitation and Western blotting of tagged transgenic proteins
P. falciparum parasitized erythrocyte lysates were prepared from cultures at 8–10% parasitemia. Proteins were extracted and immunopurified using anti-GFP or anti-RFP beads (MBL), separated by SDS-PAGE and either processed for LC-MS/MS or Western blotting. See Extended Experimental Procedures for details.
Selective permeabilization of infected erythrocytes
Saponin was used to selectively permeabilize the infected erythrocyte membrane and the PVM (but not the parasite plasma membrane) of P. falciparum-infected erythrocytes. For selective permeabilization of just the infected erythrocyte membrane, 100 U of tetanolysin was used. See Extended Experimental Procedures for additional details.
Mass Spectrometry and peptide quantitation
Protein bands of interest were excised, digested with AspN and analyzed by LC-MS/MS using reverse phase capillary HPLC with a Thermo Electron LTQ OrbiTrap XL mass spectrometer. The peptide identities of integrated peaks were verified based on retention times of matching MS/MS spectra. See Extended Experimental Procedures for additional details.
Immunolocalization by indirect immunofluorescence assays (IFA) and immunoelectron microscopy
IFAs were performed on parasites fixed with glutaraldehyde/paraformaldehyde, using indicated primary and secondary antibodies and slides were stained with Hoechst 33342. See Extended Experimental Procedures for additional details.
For immunoelectron microscopy, infected erythrocytes were fixed and embedded in LR white. Thin sections were probed with rabbit anti-mCherry (single labeling) or mouse anti-mCherry and rabbit anti-BIP/ anti-pre-HT, followed by gold conjugated secondary antibodies (10 nm and/or 15 nm). See Extended Experimental Procedures for additional details
Liposome Pelleting Assay
The liposome sedimentation assays were performed by generating liposomes with POPC, POPE, and/or phosphoinositides. Liposomes were then mixed with proteins to yield solutions containing 1 mM total lipid and 5 μM proteins. Bound proteins were eluted with Laemmli buffer, resolved by SDS-PAGE and developed with Coomassie dye. See Extended Experimental Procedures for additional details.
SPR Binding Protein-Lipid Interactions
All SPR measurements were performed at 25°C in PBS. Sensor chip surfaces were injected with POPC/POPE/Phosphoinositide (77:20:5) and POPC/POPE (80:20) vesicles at 5 μl/min onto the active surface and the control surface, respectively, to give the same resonance unit (6000 RU) values. Equilibrium SPR measurements were done at the flow rate of 5 μl/min. Each data set was repeated three times to calculate the mean ± standard deviation. See Extended Experimental Procedures for additional details.
Immunoprecipitation using Pre-HT and Post-HT antibodies and detection of PI(3)P by ELISA
P. falciparum 3D7 infected cells were cultured with 50 μM HIV protease inhibitor Lopinavir (Selleckchem), to inhibit plasmepsin V activity, for 7 h and cell lysates were subjected to immunoprecipitation. The relative amounts of pPfHRPII (immunoprecipitated by pre-HT antibodies), and mPfHRPII (immunoprecipitated by post-HT antibodies) was determined by Western blotting with a mouse anti-PfHRPII and densitometry (data not shown). Equal amounts of pPfHRPII and mPfHRPII were used to detect PI(3)P binding by SPR and estimate bound cellular PI(3)P. See Extended Experimental Procedures for additional details.
HIGHLIGHTS
  • [triangle]
    PI(3)P-enriched regions are detected in the malaria endoplasmic reticulum.
  • [triangle]
    signals of a P. falciparum `secretome' bind to PI(3)P with nanomolar affinity.
  • [triangle]
    Endogenous, non-cleaved HT signal on ER precursors binds PI(3)P in the ER.
  • [triangle]
    PI(3)P binding in the ER and not HT signal cleavage is needed for host cell export.
Supplementary Material
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ACKNOWLEDGEMENTS
This project was conceived by K.H, R.V.S and S.B., and the experiments were planned by K.H, R.V.S, S.B, D.W.S and K.D.S. S.B, R.V.S and K.D.S conducted the experiments. K.H, R.V.S and S.B wrote the paper with input from all authors. We thank Yi Xue and Jordan L. Scott for excellent technical assistance, Andrew Osborne (University College of London) for helpful discussions and Caroline Furtado Junqueira for help in figure representation. We would also like to thank Lennell Reynolds Jr., the Cell Imaging Facility at Northwestern University and William Archer at the University of Notre Dame for help with electron microscopy This work was partially supported by NIH grants HL069630, AI039071, HL078826 (K.H); AI081077 (R.V.S); HL038794 (D.W.S) and CA10815 (Wistar Proteomics Core Facility).
Footnotes
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SUPPLEMENTAL INFORMATION Supplementation information includes Extended Experimental Procedures, Supplemental References, three Supplemental Tables and six Supplemental Figures.
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