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Nucleic Acids Res. 1981 April 10; 9(7): 1707–1721.
PMCID: PMC326792

Monoclonal antibodies showing sequence specificity in their interaction with single-stranded DNAs.

Abstract

Six hybridoma cell lines which secrete monoclonal antibodies binding to nucleic acids were produced from autoimmune NZB/NZW mice. Four of the antibodies were IgG's and the other two were IgM's. Using a solid phase radioimmunoassay (SPRIA) the binding of the antibodies to over thirty different nucleic acids was estimated. All the antibodies were extremely specific. There was no detectable interaction with various RNAs, and single-stranded DNAs bound more antibodies than duplex or multi-stranded DNAs. In every case the antibodies also showed considerable sequence preferences. For example one monoclonal antibody bound to d(TTC)n but not to d(TCC)n while another interacted strongly with D(TG)n and d(CA)n but not with d(TC)n, d(GA)n or homopolymers. In other cases the patterns of sequence specificity were extremely difficult to interpret although it seems clear that monoclonal antibodies have the potential to distinguish between any two nucleic acids however similar.

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Selected References

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  • Koffler D, Agnello V, Kimkel HG. Polynucleotide immune complexes in serum and glomeruli of patients with systemic lupus erythematosus. Am J Pathol. 1974 Jan;74(1):109–124. [PubMed]
  • Politz SM, Glitz DG. Ribosome structure: localization of N6,N6-dimethyladenosine by electron microscopy of a ribosome-antibody complex. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1468–1472. [PubMed]
  • Rudkin GT, Stollar BD. High resolution detection of DNA-RNA hybrids in situ by indirect immunofluorescence. Nature. 1977 Feb 3;265(5593):472–473. [PubMed]
  • Stollar BD. The specificity and applications of antibodies to helical nucleic acids. CRC Crit Rev Biochem. 1975 May;3(1):45–69. [PubMed]
  • Talal N, Steinberg AD. The pathogenesis of autoimmunity in New Zealand black mice. Curr Top Microbiol Immunol. 1974;64(0):79–103. [PubMed]
  • Mosmann TR, Baumal R, Williamson AR. Mutations affecting immunoglobulin light chain secretion by myeloma cells. I. Functional analysis by cell fusion. Eur J Immunol. 1979 Jul;9(7):511–516. [PubMed]
  • Mosmann TR, Gravel Y, Williamson AR, Baumal R. Modification and fate of J chain in myeloma cells in the presence and absence of polymeric immunoglobulin secretion. Eur J Immunol. 1978 Feb;8(2):94–101. [PubMed]
  • McConahey PJ, Dixon FJ. Radioiodination of proteins by the use of the chloramine-T method. Methods Enzymol. 1980;70(A):210–213. [PubMed]
  • Morgan AR, Lee JS, Pulleyblank DE, Murray NL, Evans DH. Review: ethidium fluorescence assays. Part 1. Physicochemical studies. Nucleic Acids Res. 1979 Oct 10;7(3):547–569. [PMC free article] [PubMed]
  • Lee JS, Evans DH, Morgan AR. Polypurine DNAs and RNAs form secondary structures which may be tetra-stranded. Nucleic Acids Res. 1980 Sep 25;8(18):4305–4320. [PMC free article] [PubMed]
  • Harwood SJ, Wells RD. Micrococcus luteus deoxyribonucleic acid polymerase. Studies on the initiation of deoxyribonucleic acid synthesis in vitro. J Biol Chem. 1970 Nov 10;245(21):5625–5634. [PubMed]
  • Khan SA, Humayun MZ, Jacob TM. Antibodies specific to a deoxyribodinucleotide sequence. Nucleic Acids Res. 1977 Sep;4(9):2997–3006. [PMC free article] [PubMed]
  • Eilat D, Asofsky R, Laskov R. A hybridoma from an autoimmune NZB/NZW mouse producing monoclonal antibody to ribosomal-RNA. J Immunol. 1980 Feb;124(2):766–768. [PubMed]
  • Tron F, Charron D, Bach JF, Talal N. Establishment and characterization of a murine hybridoma secreting monoclonal anti-DNA autoantibody. J Immunol. 1980 Dec;125(6):2805–2809. [PubMed]
  • Andrzejewski C, Jr, Stollar BD, Lalor TM, Schwartz RS. Hybridoma autoantibodies to DNA. J Immunol. 1980 Mar;124(3):1499–1502. [PubMed]

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