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Logo of bttDove Medical PressThis ArticleSubscribeSubmit a ManuscriptSearchFollowDovepressBiologics: Targets and Therapy
 
Biologics. 2012; 6: 1–4.
Published online Dec 22, 2011. doi:  10.2147/BTT.S27582
PMCID: PMC3266860
Prevalence of OmpK35 and OmpK36 porin expression in beta-lactamase and non-betalactamase- producing Klebsiella pneumoniae
Pegah Shakib,1,2 Sobhan Ghafourian,1 Mohammad Reza Zolfaghary,2 Reza Hushmandfar,3 Reza Ranjbar,4 and Nourkhoda Sadeghifard1
1Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam
2Department of Microbiology, Qom Branch, Islamic Azad University of Qom, Qom
3Faculty of Veterinary Medicine, Ilam University, Ilam
4Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Correspondence: Nourkhoda Sadeghifard, Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran, Tel +98 91 8746 5523, Email sadeghifard/at/gmail.com
Abstract
Background
The aims of this study were to confirm the presence of OmpK35 and OmpK36 in extended-spectrum beta-lactamase-producing and nonextended-spectrum beta-lactamase-producing Klebsiella pneumoniae and to determine the relationship between porin expression and resistance to third-generation cephalosporins.
Methods
Fifty-two K. pneumoniae isolates were obtained and analyzed for extended-spectrum beta-lactamase and for OmpK35 and OmpK36.
Results
Twenty-two (42.3%) isolates of K. pneumoniae were extended-spectrum beta-lactamase producers. The OmpK35 profile in K. pneumoniae producing extended-spectrum beta-lactamase showed the presence of porin protein in ceftazidime-sensitive K. pneumoniae (six isolates), and the OmpK36 profile in K. pneumoniae producing extended-spectrum beta-lactamase revealed isolates sensitive to cefotaxime (n = 8) and ceftriaxone (n = 6). All nonextended-spectrum beta-lactamase-producing K. pneumoniae showed the presence of OmpK35 and OmpK36 porin proteins.
Conclusion
The presence of OmpK35 is mostly related to ceftazidime susceptibility and less to cefotaxime and ceftriaxone susceptibility, while OmpK36 expression is seen more often in cefotaxime-sensitive isolates. OmpK35 and OmpK36 indicate nonextended-spectrum beta-lactamase producing strains, and their presence is important when selecting an antimicrobial agent.
Keywords: Klebsiella pneumoniae, extended-spectrum beta-lactamase producing strains, porin expression
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