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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Cell Host Microbe. Author manuscript; available in PMC Jan 19, 2013.
Published in final edited form as:
PMCID: PMC3266541
NIHMSID: NIHMS345631
The Legionella pneumophila effector DrrA is sufficient to stimulate SNARE-dependent membrane fusion
Kohei Arasaki,1,2 Derek K. Toomre,3 and Craig R. Roy1*
1Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06526
2School of Life Sciences, Tokyo University of Pharmacy and Life Sciences Horinouchi, Hachioji, Tokyo 192-0392, Japan
3Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06535
*Correspondence: craig.roy/at/yale.edu
The intracellular bacterial pathogen Legionella pneumophila subverts host membrane transport pathways to promote fusion of vesicles exiting the endoplasmic reticulum (ER) with the pathogen-containing vacuole. During infection there is non-canonical pairing of the SNARE protein Sec22b on ER-derived vesicles with plasma membrane (PM)-localized syntaxin proteins on the vacuole. We show that the L. pneumophila Rab1-targeting effector DrrA is sufficient to stimulate this non-canonical SNARE association and promote membrane fusion. DrrA activation of the Rab1 GTPase on PM-derived organelles stimulated the tethering of ER-derived vesicles with the PM-derived organelle resulting in vesicle fusion through the pairing of Sec22b with the PM syntaxin proteins. Thus, the effector protein DrrA stimulates a host membrane transport pathway that enables ER-derived vesicles to remodel a PM-derived organelle, suggesting that Rab1 activation at the PM is sufficient to promote the recruitment and fusion of ER-derived vesicles.
Keywords: Legionella pneumophila, Dot/Icm, DrrA/SidM, SNARE, membrane fusion, syntaxin, Sec22b, plasma membrane and ER-derived vesicle
Legionella pneumophila is a gram-negative bacterium that has the ability to replicate in phagocytes by creating a vacuole that avoids endocytic maturation (Horwitz, 1983). ER-derived vesicles are rapidly recruited and remodel the PM-derived organelle harboring Legionella by a process mediated by the type IV secretion system called Dot/Icm (Berger and Isberg, 1993; Roy et al., 1998; Segal et al., 1998; Swanson and Isberg, 1995; Tilney et al., 2001; Vogel et al., 1998) }.
The Dot/Icm apparatus delivers bacterial proteins known as effectors into the host cell (Nagai et al., 2002). The Legionella genome encodes over 200 proteins translocated into host cells by the Dot/Icm system (Ensminger and Isberg, 2009). Although the biochemical activities of most effectors remain to be determined, the few effectors that have been studied in detail include proteins that modulate the function of GTPases that regulate host membrane transport. These include the protein RalF, which is an Arf guanine nucleotide exchange factor (GEF) (Nagai et al., 2002), and several proteins that modulate Rab1 function (Ingmundson et al., 2007; Machner and Isberg, 2006, 2007; Murata et al., 2006).
The DrrA protein (also called SidM) is a well-characterized effector targeting Rab1 (Ingmundson et al., 2007; Machner and Isberg, 2006, 2007; Murata et al., 2006). Three functional domains in DrrA (Figure 1A) have been defined biochemically and resolved structurally (Muller et al., 2010; Schoebel et al., 2009; Suh et al., 2009; Zhu et al., 2010). The carboxyl-terminal region of DrrA contains a phosphatidylinositol 4-phosphate (PI4P) binding domain (Brombacher et al., 2009; Schoebel et al., 2010; Zhu et al., 2010) that targets DrrA to the host PM (Murata et al., 2006). The central region of DrrA contains a Rab1-specific GEF domain that can displace Rab-GDI bound to inactive Rab1 (Schoebel et al., 2009; Suh et al., 2009; Zhu et al., 2010). The amino-terminal region of DrrA has an adenylyltransferase (ATase) domain that uses ATP as a substrate to attach an AMP residue to a conserved tyrosine residue in the switch 2 region of activated Rab1-GTP, making Rab1 insensitive to GTPase-activating proteins (GAPs) (Muller et al., 2010). Thus, DrrA mediates the recruitment and activation of Rab1 on the PM-derived vacuole that harbors Legionella.
Figure 1
Figure 1
DrrA associates with PM syntaxins
Although mutations that eliminate the DrrA protein reduce the amount of Rab1 recruited to the LCV, the ability of Legionella to traffic correctly and remodel the Legionella-containing vacuole (LCV) is not compromised to a measurable degree in a drrA mutant, presumably because of the functional redundancy inherent in such a large repertoire of bacterial effectors (Ingmundson et al., 2007; Machner and Isberg, 2007). Functional redundancy has made it difficult to assess the contribution of individual effectors in LCV transport using standard genetic techniques. Thus, it is unclear why Legionella has acquired DrrA.
The molecular details of how ER-derived vesicles fuse with the LCV remain unknown, but host proteins involved in the transport of early secretory vesicles are involved. In addition to a possible role for Rab1 (Derre and Isberg, 2004; Kagan et al., 2004), membrane fusion between the LCV and ER-derived vesicles involves interactions between the v-SNARE Sec22b on the ER-derived vesicles and a PM t-SNARE complex containing host syntaxins (Arasaki and Roy, 2010). Here, we show that the DrrA protein promotes the tethering of ER-derived vesicles with the PM-derived organelle, which leads to membrane fusion through Sec22b interactions with PM-localized syntaxins.
DrrA interacts with PM syntaxins
When coexpressed in mammalian cells the GFP-tagged DrrA61-647 protein (Figure 1A) was detected in a coprecipitate with 3x-FLAG-tagged PM syntaxins (Stx2, Stx3, and Stx4) at higher levels compared to SNAREs SNAP23, Sec22b or the Golgi-localized syntaxin 3x-FLAG-Stx5 (Figure 1B). The DrrA61-647 protein was used because it does not display host toxicity associated with the ATase domain but retains GEF and PI4P-binding activities (Figure 1A). The C-terminal region of DrrA containing a PI4P-binding domain that mediates PM localization was sufficient for the syntaxin interaction, as GFP-DrrA451-647 coprecipitated more efficiently with the PM syntaxins compared to the control SNARE proteins SNAP23 or Sec22b (Figure 1C). GFP-tagged DrrA451-647 coprecipitated with cytosolic derivatives of Stx2, Stx3 and Stx4 lacking their transmembrane domain (Figure 1D). By contrast, GFP-DrrA61-647 protein did not efficiently interact with the cytosolic derivatives of these syntaxins (Figure 1D), suggesting an interaction between the DrrA61-647 protein and these syntaxins requires additional factors at the membrane surface.
After infection of host cells by wild-type Legionella, endogenous DrrA was detected in association with 3x-FLAG-Stx3, whereas, DrrA was not detected in association with 3x-FLAG-Stx3 in cells infected with a dotA mutant strain that is unable to translocate DrrA into cells or in cells infected with the ΔdrrA mutant (Figure 2A). Consistent with results using GFP-DrrA61-647 expressed ectopically (Figure 1A), endogenous DrrA translocated during infection showed the highest affinity for 3x-FLAG-Stx3 followed by Stx4 (Figure 2B). An interaction between 3x-FLAG-Stx2 and translocated DrrA was below the threshold of detection in this assay (Figure 2B), consistent with DrrA binding to Stx2 being less efficient than Stx3 and Stx4 binding. Additionally, translocated DrrA was not detected in association with control proteins SNARE proteins SNAP23 or VAMP7. Thus, after translocation into host cells by Legionella the DrrA proteins associates with a complex containing PM syntaxins.
Figure 2
Figure 2
DrrA translocated into host cells during infection associates with PM syntaxins
Rab1 facilitates binding of DrrA to Stx3
The ectodomain of syntaxin proteins contains a regulatory Habc region that binds the SNARE motif through an intramolecular interaction and chaperone proteins such as Munc18 bind to the Habc region to regulate SNARE interactions (Dietrich et al., 2003). To define regions required for interaction and understand the mechanisms that regulate these interactions, binding of DrrA to deletion derivatives of PM syntaxin proteins were examined by coprecipitation. These in vivo data revealed that the SNARE motif in the PM syntaxins was required for formation of a complex containing the GFP-DrrA451-647 protein (Figure S1). To determine if DrrA interacts directly with the SNARE motif, MBP-tagged derivatives of Stx3 and His-tagged derivatives of DrrA were purified and tested for interaction in vitro. A direct interaction was observed between DrrA451-647 and Stx3 derivatives containing the SNARE motif (Figure 3A, MBP-Stx3 ΔHab/TMD and MBP-Stx3 ΔHabc/TMD). DrrA1-500 did not interact with the Stx3 proteins, indicating that the C-terminal domain of DrrA is both sufficient and necessary for binding. The intact Habc region in Stx3 prevented this in vitro interaction (Figure 3A, MBP-Stx3 ΔTMD), similar to what was observed with the positive control protein SNAP23, which is a cognate SNARE that interacts with Stx3 through SNARE motif interactions. This suggests that the Habc region and DrrA451-647 have overlapping binding sites in the SNARE motif, and that chaperone proteins inside the cell that bind to the Habc domain can expose the SNARE motif for DrrA recognition in vivo, enabling DrrA to interact with the full-length Stx3 protein in cells. The interaction between DrrA451-647 and MBP-Stx5 ΔHab/TMD and MBP-Stx5 ΔHabc/TMD was lower compared to the analogous Stx3 interactions, indicating that DrrA binds preferentially to the SNARE motif of Stx3 (Figure 3B).
Figure 3
Figure 3
Rab1 regulates DrrA binding to the SNARE motif in Stx3
When compared to DrrA451-647, the full-length DrrA1-647 protein was less efficient at forming a complex with the SNARE motif of Stx3 under these in vitro conditions (Figure 3A). One explanation is that the central region of DrrA in the context of the full-length protein regulates the ability of the C-terminal region to interact with syntaxins by conferring an auto-inhibitory function that would be relieved in vivo during Rab1 binding, which is consistent with the in vivo data (Figure 1D). This suggests that an interaction between the DrrA61-647 protein and these syntaxins could be regulated by factors at the membrane surface. Consistent with this hypothesis, incubation of full-length DrrA with the GDP-locked variant of Rab1 (GST-Rab1S25N), which was shown previously to bind tightly to DrrA (Ingmundson et al., 2007; Machner and Isberg, 2006, 2007; Murata et al., 2006), enhanced complex formation between DrrA and Stx3 (Figure 3C). By contrast, the addition of the GTP-locked variant Rab1Q70L, which does not bind DrrA tightly, did not enhance Stx3 complex formation (Figure 3C). Similarly, when wild type Rab1 was added to DrrA in the absence of GTP to prevent nucleotide exchange, enhanced complex formation between full-length DrrA and Stx3 was observed in manner that was dependent on the amount of Rab1 in the reaction (Figure 3C-E). By contrast, the binding of DrrA to Stx3 in vitro was reduced under condition where the stable association of Rab1 and full-length DrrA was abrogated by the inclusion of GTP (Figure 3C,D).
Because the ATase domain of DrrA can use GTP as a substrate to GMPylate Rab1 (Muller et al., 2010), the full length DrrA protein was compared in this assay to the DrrA340-647 protein, which has the ATase domain deleted but retains an intact GEF and PI4P binding domain. The full-length DrrA protein and the DrrA340-647 protein gave similar results in this assay (Figure 3D, compare DrrA1-647 to DrrA340-647), which demonstrates that the inactive Rab1 protein enhanced binding of full-length DrrA to Stx3 observed in this in vitro assay was dependent on stable interactions between Rab1 and the GEF domain of DrrA and not on Rab1 GMPylation mediated by the ATase domain. These results suggest that a region near the carboxyl terminus of DrrA interacts with Stx3, and Rab1 binding to the full-length DrrA protein facilitates this interaction.
DrrA promotes non-canonical SNARE pairing
DrrA and Stx3 association was coordinated by Rab1 activation, further supporting that DrrA might promote Rab1-dependent membrane tethering and fusion events. Given data indicating that Legionella infection promotes a functional interaction between Stx3 and Sec22b, we asked whether DrrA was sufficient to promote this non-canonical SNARE interaction. Vesicles derived from the ER were isolated in a post nuclear supernatant (PNS) fraction from HEK293-FcγRII cells that stably express 3x-FLAG-Sec22b. Amylose beads containing MBP-Stx3, DrrA, and Rab1 or MBP-Stx3 and DrrA451-647 were incubated with the PNS fraction with or without GDP or GTP. The pairing of Sec22b on the vesicles with purified MBP-Stx3 in the reaction was measured by coprecipitation of proteins associated with MBP-Stx3 on the amylose beads. These data demonstrate that in the reaction containing full-length DrrA and GTP, the binding of MBP-Stx3 to the 3x-FLAG-Sec22b associated with the vesicles was enhanced more than five fold (Figure 4, ,3x-FLAG-Sec22b3x-FLAG-Sec22b lane), indicating that Rab1 activation by DrrA stimulated the association of Stx3 and Sec22b.
Figure 4
Figure 4
DrrA and Rab1 coordinate Stx3 interactions with Sec22b
A digitonin permeabilization system (Traub et al., 1996) that allows the diffusion of untethered vesicles across the PM of semi-intact cells (Aoki et al., 2009; Jesch and Linstedt, 1998; Zolov and Lupashin, 2005) was used to further investigate DrrA-mediated assembly of a Sec22b and Stx3 SNARE complex. Cells producing both GFP-Stx3 and derivatives of GFP-DrrA were permeabilized partially with digitonin and incubated with a PNS fraction containing ER-derived vesicles from cells producing 3x-FLAG-Sec22b. The binding of vesicle-associated Sec22b to PM-localized GFP-Stx3 was analyzed by coprecipitation. A GTP-dependent interaction between GFP-Stx3 and 3x-FLAG-Sec22b was detected in cells producing derivatives of DrrA with an intact GEF domain and PI4P-binding domain (Figure 5A, GFP-DrrA61-647 and GFP-DrrA201-647). By contrast, GFP-DrrA451-647, which does not have an intact GEF domain but retains the PI4P-binding domain and has the ability to interact with Stx3, did not promote an interaction between Stx3 and Sec22b, supporting that Rab1 activation by DrrA is important for stimulating formation of this SNARE complex (Figure 5A). Because functional SNARE pairs are recognized and dissociated by a complex consisting of αSNAP and NSF, purified NSF and α-SNAP were added to the reactions before the immunoprecipitation of 3x-FLAG-Sec22b to determine whether these factors dissociated Stx3 from the complex. The addition of NSF and α-SNAP resulted in a reduction in the association of Stx3 and Sec22b, indicating that the complex formed upon the addition of DrrA represents a functional SNARE assembly (Figure 5B). Thus, the ability of DrrA to recruit and activate Rab1 at the PM can initiate the assembly of a SNARE complex containing Stx3 and Sec22b.
Figure 5
Figure 5
DrrA coordinates the functional pairing of Stx3 and Sec22b in permeabilized cells
DrrA activation of Rab1 promotes the tethering ER-derived vesicles with PM-derived organelles
Rab proteins play an important role in promoting the tethering of vesicles to target membranes, and this will promote membrane fusion if compatible SNARE proteins are present on juxtaposed membranes. Our data suggest that ER-derived vesicles are recruited to PM-derived organelles following DrrA-mediated activation of Rab1, and that tethering of these vesicles promotes this non-canonical SNARE interaction. To investigate this further, cells permeabilized with digitonin were incubated with PNS from cells producing a YFP-KDEL protein localized to the lumen of ER-derived vesicles (Figure S2A). Fluorescence microscopy showed that DrrA stimulated the tethering of ER-derived vesicles containing YFP-KDEL with the permeabilized cells (Figure S2B). This tethering of the ER-derived vesicles required the GEF domain in DrrA and the addition of GTP, suggesting that Rab1 activation is important for the tethering reaction (Figure S2B). Silencing of host PM syntaxins by siRNA in the permeabilized acceptor cells did not affect DrrA-mediated tethering of the ER-derived vesicles (Figure S2C), consistent with vesicle tethering being independent of SNARE interactions. Upon addition of a “fusion buffer” consisting of a fresh cytosolic protein extract and abundant levels of ATP, there was a loss in the YFP-KDEL fluorescence that required the expression of PM syntaxins in the acceptor cells (Figure S2C), These data are consistent with SNARE-mediated fusion with the PM resulting in release of the YFP-KDEL from the lumen of the ER-derived vesicles.
Tethering of ER-derived vesicles at the PM was also visualized using total internal reflection fluorescence (TIRF) microscopy. Single ER-derived vesicles labeled with RFP-KDEL were observed tethered in an induced evanescent field at the PM of permeabilized cells by a process requiring DrrA and GTP (Figure 6 and S2D). Upon the addition of fusion buffer, most vesicles lost fluorescence over a reaction period of 30 sec, suggesting fusion of the vesicles with the PM resulted in release of the luminal RFP-KDEL probe from the tethered vesicles into the extracellular environment. Silencing of the PM syntaxins in the acceptor cells, or silencing of Sec22b on ER-derived vesicles obtained from the donor cells, did not affect the tethering reaction (Figure 6 and S2E), but did interfere with the loss in fluorescence observed upon the addition of fusion buffer (Figure 6). Thus, Rab1 activation by DrrA promotes the tethering of ER-derived vesicles to a PM-derived organelle, and Sec22b interactions with the PM syntaxins potentially promote vesicle fusion.
Figure 6
Figure 6
DrrA alters ER-derived vesicle dynamics at the PM
DrrA activates a host pathway that promotes the fusion of ER-derived vesicles with a PM-derived organelle
To corroborate that the loss in fluorescence observed by TIRF microscopy was a valid indicator of membrane fusion, donor vesicles with soluble Luciferase-KDEL restricted to the lumen were employed in the permeabilized cell assay. Fusion of the ER-derived vesicles with the PM should release the soluble Luciferase-KDEL protein contained in the lumen of the tethered vesicles. Thus, fusion will result in an increase in the ratio of Luciferase-KDEL in the soluble extracellular fraction compared to the membrane pellet. Similar to the imaging data, DrrA stimulated donor vesicles containing Luciferase-KDEL to associate with acceptor membranes by a process requiring GTP, as indicated by the increase in Luciferase activity associated with permeabilized cells following the tethering reaction (Figure 7A). A requirement for Rab1 in the tethering reaction was indicated by a reduction in vesicle association upon the addition of GDP-locked Rab1 to the reaction, and by using PNS obtained from cells in which Rab1 had been silenced by siRNA (Figure S3A-D). Silencing of host PM syntaxins or Sec22b did not reduce DrrA-mediated tethering of ER-derived vesicles with the permeabilized cells (Figure 7B). The addition of fusion buffer stimulated release of Luciferase from the lumen of tethered ER-derived vesicles into the supernatant fraction of the reaction (Figure 7B). Luciferase release was blocked by NEM (Figure S3E), which prevents SNARE-mediated fusion reactions by interfering with the disassembly of cis-SNARE complexes by NSF and α-SNAP. These data are consistent with the release of Luciferase from the ER-derived vesicles being the result of PM fusion.
Figure 7
Figure 7
SNARE programming by DrrA and Rab1 promotes fusion of ER-derived vesicles with the PM
If the Luciferase release represents a bone fide SNARE-mediated membrane fusion event, it should require specific SNARE proteins oriented correctly on the vesicles and target membranes. Indeed, silencing of Sec22b on donor vesicles containing Luciferase-KDEL interfered with fusion, whereas, silencing of the PM syntaxins in the donor cells had no effect on fusion (Figure 7B). By contrast, silencing of the ER-localized syntaxin proteins Stx17 or Stx18 in the donor cells did not affect fusion (Figure S3F), indicating a specific role for Sec22b on the ER-derived vesicles in the fusion reaction. Similarly, silencing of PM syntaxins in the acceptor cells interfered with fusion of the ER-derived vesicles, but silencing of Sec22b in the acceptor cells had no significant impact on membrane fusion (Figure 7B). Thus, the fusion process was specific for Sec22b being the v-SNARE on the ER-derived vesicles and PM syntaxins functioning as a t-SNARE.
To independently validate that DrrA was directing the tethering and fusion of ER-derived vesicles with the PM, a GFP-tagged derivative of a temperature-sensitive vesicular stomatitis virus glycoprotein (ts045-VSVG) was used in the permeabilized cell assay to determine whether this transmembrane protein could be delivered from ER-derived vesicles to the PM. Fluorescent vesicles were obtained at different times after cells were shifted to a temperature that permits ts045-VSVG-GFP to exit the ER. The ts045-VSVG-GFP protein was localized primarily to pre-Golgi compartments 15 minutes after incubating cells at the permissive temperature (Figure S3G), and vesicles generated from these cells could be tethered to semi-intact cells in the presence of DrrA (Figure S3H). By contrast, ts045-VSVG-GFP was localized primarily to post-Golgi compartments 90 minutes after incubating cells at the permissive temperature and fluorescent vesicles generated from these cells were not tethered to semi-intact cells incubated with DrrA (Figure S3H). This indicates that DrrA preferentially directs the tethering of ER-derived vesicles to target membranes. TIRF microscopy validated that the vesicles containing ts045-VSVG-GFP were associated with the PM and that tethering was not affected by the silencing of PM syntaxins (Figure 7C, before fusion). After addition of fusion buffer (Figure 7C, after fusion), there was a redistribution of ts045-VSVG-GFP from the tethered vesicles to the PM of the recipient cells (no siRNA), which is consistent with fusion of the ER-derived vesicles. By contrast, ts045-VSVG-GFP remained localized to the tethered vesicles when PM syntaxins were silenced in the recipient cells (Stx2/3/4 KD). Thus, DrrA-mediated activation of Rab1 at the PM is sufficient to recruit ER-derived vesicles and promote their fusion through the non-canonical pairing of Sec22b and PM syntaxins (Figure 7D).
A working model summarizes our principle findings of how DrrA directs the fusion of ER-derived vesicles with the PM-derived organelle containing Legionella and underscores new open quesitons (Figure 7D). Stage 1 depicts interactions mediated by the GEF domain and the PI4P-binding domain of DrrA. Our data indicate that the DrrA carboxy-terminal region interacts directly with PM-localized syntaxins (Figure 1), providing an additional function to a domain that was previously shown to have a PI4P binding activity important for localization of DrrA to PM-derived organelles (Brombacher et al., 2009; Schoebel et al., 2010; Zhu et al., 2010). The interaction with Stx3 and the full-length DrrA protein was observed upon infection of Legionella (Figure 2), and could be enhanced in vitro by the binding of inactive GDP-bound Rab1 to the GEF domain (Figure 3). The dynamics of Rab1 activation by the GEF domain in DrrA leads to the accumulation of Rab1-GTP on the PM-derived compartment and interactions mediated by the PI4P-binding domain in DrrA would facilitate the localization of syntaxins to this region of the membrane. Thus, these activities of DrrA serve to localize host factors that participate in the tethering and fusion of ER-derived vesicles with the PM-derived organelle.
Stage 2 depicts the Rab1-dependent tethering of ER-derived vesicles to the PM-derived compartment. This stage is supported by data showing in permeabilized cells that the tethering of ER-derived vesicles to the PM required DrrA with a functional GEF domain, Rab1, and GTP (Figures S3-S5 and Figure 7A). A role for SNARE proteins in the tethering stage was not apparent, as silencing of either Sec22b or PM syntaxins did not reduce vesicle tethering stimulated by DrrA. Given that Rab-mediated vesicle tethering in cells usually involves a protein that binds to a GTP-bound Rab protein on the acceptor membrane and associates with factors on the membrane of the donor vesicle, an unidentified tethering factor that binds to active Rab1 and interacts with the ER-derived vesicle is depicted in this model. This protein could be a factor that normally associates with early secretory vesicles and binds active Rab1 on Golgi or pre-Golgi membranes. Alternatively, this tethering factor could be a protein that is not typically associated with early secretory vesicles. Future studies are needed to determine the identity of this putative tethering factor and other host factors that may participate in this Rab1-mediated tethering reaction.
Stage 3 depicts the functional association of Sec22b on the ER-derived vesicles with the PM Stx3 protein. Our data indicate that formation of this complex can be stimulated by DrrA-mediated activation of Rab1 (Figure 4). Interestingly, homologues of these SNARE proteins from Saccharomyces cerevisiae promote membrane fusion in vitro when incorporated into synthetic vesicles, however, it was argued that fusion of ER-derived vesicles with the PM would be restricted topologically by cellular mechanisms that regulate vesicle tethering in vivo (McNew et al., 2000). Consistent with this hypothesis, our model predicts that the tethering reaction mediated by activated Rab1 at the PM is sufficient to overcome this topological restriction and promote functional interactions between Sec22b and PM syntaxins. Thus, the primary role of DrrA in promoting the tethering of ER-derived vesicles with the PM is to overcome this topological restriction by activating Rab1 on this organelle.
Stage 4 depicts the fusion of ER-derived vesicles with the PM-derived organelle. Independent indicators of this fusion reaction are provided by data showing the DrrA-mediated release of the soluble Luciferase-KDEL protein contained in the lumen of ER-derived vesicles and the delivery of the transmembrane protein ts045-VSVG-GFP from the limiting membrane of ER-derived vesicles to the surface of cells (Figure 7). Fusion of ER-derived vesicles with the PM resulting from DrrA-mediated activation of Rab1 was blocked when PM syntaxins were reduced on the acceptor compartment or when Sec22b was reduced on the donor compartments, demonstrating fusion involves a topological requirement for these SNAREs on distinct organelles. SNAP23 is depicted as also being involved in this fusion process based on data showing fusion of synthetic liposomes with a t-SNARE complex consisting of the SNAP23 homologue Sec9p and the PM syntaxin homologue Sso1p with liposomes that display the v-SNARE Sec22p (McNew et al., 2000).
Importantly, the observation that DrrA was sufficient to promote the tethering and fusion of ER-derived vesicles with the PM indicates that no additional bacterial factors are required to promote this reaction. In the context of Legionella infection, however, other effector proteins are predicted to enhance the efficiency of this pathway. Notably, the Legionella effector LidA binds to Rab1, and immobilization of LidA and DrrA to paramagnetic beads is sufficient to stimulate the tethering of ER-derived vesicles to beads by a Rab1-dependent mechanism (Machner and Isberg, 2006, 2007). Thus, LidA should enhance DrrA-mediated fusion of ER-derived vesicles with the PM-derived organelle. Additionally, the observation that Dot/Icm-dependent Sec22b and Stx3 interactions are detected during infection of host cells by a ΔdrrA mutant (Arasaki and Roy, 2010) indicate that there should be Legionella effectors that promote tethering of ER-derived vesicles to the LCV membrane by a process that does not require DrrA function. Possibilities include effectors that mimic active Rab1 or mimic the tethering factors Rab1 recruits to the LCV. Tethering mediated by these effectors would then promote the engagement of PM syntaxins and Sec22b, which would promote fusion of ER-derived vesicles with the LCV by a pathway that is independent of DrrA.
Lastly, these data may provide insight into cellular process that could direct the fusion of ER-derived vesicles with phagosome membranes during endocytic maturation. There are several independent studies that suggest the existence of an endogenous cellular pathway in phagocytic cells, such as macrophages, that enables ER-derived vesicles to fuse with phagosomes (Becker et al., 2005; Gagnon et al., 2002; Hatsuzawa et al., 2006). Immunological studies have indicated that fusion of ER with phagosomes is important for the delivery components that allow antigens in the lumen of phagosomes to be transported across a vacuole membrane and presented on MHC class I molecules (Ackerman et al., 2006; Guermonprez et al., 2003; Houde et al., 2003). However, it is difficult to detect fusion of ER-derived membranes with phagosomes by fluorescence microscopy (Touret et al., 2005) or by quantitative mass spectrometry (Rogers and Foster, 2007), suggesting if fusion of ER-derived membranes with phagosomes represents an endogenous transport pathway in cells, this process must be tightly regulated. Our data suggest that activation of Rab1 on the phagosome membrane would be sufficient to promote the recruitment of ER-derived vesicles, and fusion of these vesicles with the phagosomes would occur upon formation of the SNARE complex containing Sec22b and PM syntaxins. Thus, it will be interesting to know whether phagocytic cells produce a GEF that functions analogously to DrrA to regulate the recruitment and fusion of ER-derived vesicles with phagosomes, and if Sec22b and PM syntaxins are involved.
Cell culture, siRNA, transfection, bacterial strain and infection
Maintenance of HEK293-FcγRII cells and HEK293-FcγRII 3x-FLAG-Sec22b cells were described previously (Arasaki and Roy, 2010). RNA duplexes targeting Stx2, Stx3, Stx4, Stx17, Stx18, Sec22b, Rab1A and Rab1B purchased from Dharmacon as siRNAplusSmart Pools. Transfection of plasmid DNA or siRNA was performed using LipofectAmine 2000 (Invitrogen) according to the manufacture’s recommendation. Growth of L. pneumophila strains Lp01, the dotA mutant strain CR58 and the ΔdrrA mutant were described previously (Coers et al., 2000; Murata et al., 2006; Nagai et al., 2002; Zuckman et al., 1999).
Antibodies
Rabbit polyclonal antibodies to GFP and SNAP23 were purchased from Invitrogen and Abcam, respectively. Mouse monoclonal antibodies to FLAG and His were obtained from Sigma Aldrich. Goat polyclonal antibody to GST and rabbit polyclonal antibody to MBP were purchased from GE healthcare and New England Biolabs, respectively. Rabbit polyclonal antibodies to Rab1A and Rab1B were purchased from Santa Cruz. Rabbit polyclonal antibodies to Legionella and DrrA were described (Murata et al., 2006).
Protein purification
Affinity purification of tagged proteins was conducted as described (Arasaki and Roy, 2010; Ingmundson et al., 2007; Murata et al., 2006). Proteins were dialyzed in buffer containing 100mM NaCl, 1mM MgCl2 and 20mM Hepes-KOH (pH7.2).
Infection, immunoprecipitation and in vitro binding assay
Infection of HEK293-FcγRII cells with Legionella and generation of cell lysates for immunoprecipitation studies were conducted as described (Arasaki and Roy, 2010). For immunoprecipitation studies, lysates were incubated with anti-FLAG M2 beads for 1hour at 4°C. After incubation, beads were washed extensively and 3x-FLAG fusion proteins were eluted using a 3x-FLAG peptide (final concentration; 100μg/ml). For in vitro binding assays, MBP fusion proteins were incubated with 10μl of amylose resin in binding buffer (100mM NaCl, 1mM MgCl2, 20mM Hepes-KOH (pH7.2) containing 0.2% Triton X-100) for 3 hr at 4°C. The beads were washed extensively using binding buffer to remove unbound MBP fusion proteins and then mixed with His or GST fusion proteins in binding buffer overnight. Beads were washed extensively and resuspended in SDS-PAGE sample buffer for analysis.
Preparation of permeabilized cells and PNS fractions
HEK293-FcγRII cells were grown on poly-L-lysine (MW 75,000-150,000; Sigma Aldrich) coated tissue culture plates or 35mm glass bottom dishes. Cells were washed with permeabilization buffer (125mM KOAc, 2.5mM Mg(OAc)2, 25mM Hepes-KOH (pH7.2), 1mg/ml glucose and 1mM DTT) and treated with 30μg/ml digitonin in permeabilization buffer for 5 min at room temperature. After treatment, permeablized cells were washed with permeabilization buffer. To prepare PNS fractions, cells were resuspended in homogenization buffer (125mM KOAc, 2.5mM Mg(OAc)2, 25mM Hepes-KOH (pH7.2), 0.25mM Sucrose, 1mM DTT and protease inhibitors) and disrupted using a ball bearing homogenizer. Homogenized cells were centrifuged at 1,000xg for 5 min to remove unbroken cells and cell debris and the resulting supernatant was used as the PNS fraction.
TIRF Microscopy
HEK293-FcγRII cells grown on poly-L-lysine coated 35mm glass bottom dish (1.5x105 cells/dish) were transfected with or without Stx2/3/4 siRNA. At 52 hr after transfection, cells were transfected with plasmid encoding GFP-DrrA61-647, and incubated for an additional 20 hr. Transfected cells were permeabilized as described above. To prepare donor vesicles, HEK293-FcγRII cells were transfected with or without Sec22b siRNA. At52 hr after transfection, cells were transfected with plasmid encoding RFP-KDEL for 20 hr and then cells were collected and disrupted in 700μl homogenization buffer as described above. The resulting PNS fraction (100μl) containing fluorescent vesicles was incubated with permeabilized cells in the presence of 0.5mM GTP for 90 min at room temperature. Cells were washed extensively and warmed to 37°C for 15 min on a temperature-controlled stage attached to the TIRF microscope. A field in which cells producing GFP-DrrA could be detected was selected for imaging. Time-lapse microscopy was initiated before the addition of 100μl of fusion buffer, which consisted of the PNS fraction from HEK293-FcγRII cells containing freshly added solutions of GTP and ATP to a final concentration of 0.5 mM. TIRF images were collected using an inverted microscope (IX-70; Olympus) fitted with a 60x NA 1.45 TIRFM lens (Olympus) and controlled by Andor iQ software (Andor Technology). Laser lines (488 and 568nm) from argon and argon/krypton lasers were coupled to the TIRFM condenser through a single optical fiber. The calculated evanescent field depth was ~100nm. Cells were imaged by excitation at 0.25Hz, without binning, with 0.5-s exposures and detected with a black illuminated electron-multiplying charge-coupled device camera (512x512 pixels; 16-bit; iXon887; Andor Technology). Image analysis was performed using ImageJ software (NIH).
Luciferase-KDEL recruitment and fusion assay
HEK293-FcγRII cells grown on poly-L-lysine-coated 24-well plates (1x105 cells/well) were permeabilized as describe above. Permeabilized cells were incubated with or without 3 μg of purified His-DrrA for 1 hr at room temperature. Cells were washed and 100 μl of a PNS fraction from HEK293-FcγRII cells was added either with or withoutGDP or GTP at 0.5 mM final concentration. Cells were incubated for 1 hr at room temperature and then washed extensively. To assay tethering, 100 μl of lysis buffer (100mM NaCl, 1mM MgCl2, 20mM Hepes-KOH (pH7.2), 1% Triton X-100 and protease inhibitors) was added to the cells to liberate Luciferase-KDEL from vesicles and activity was measured using a Luciferase assay kit (New England Biolabs). To measure vesicle fusion, in tethering reactions using Luciferase-KDEL vesicles and permeabilized cells conducted in parallel wells as described above. After the tethering reaction, 100μl of fusion buffer was added to the reaction, which consisted of PNS fraction from HEK293-FcγRII cells containing freshly added solutions of GTP and ATP to a final concentration of 0.5 mM. Luciferase-KDEL was collected in the reaction supernatant and samples were centrifuged at 100,000xg for 30 min to remove any intact vesicles. Luciferase-activity was measured in the cleared supernatant and compared to the Luciferase activity that remained associated with the permeabilized cells. Results were expressed as the ratio of Luciferase activity in the supernatant compared to Luciferase activity that remained cell-associated.
Ts045-VSVG-GFP recruitment and fusion assay
HEK293-FcγRII cells were transfected with a plasmid encoding ts045-VSVG-GFP (Presley et al., 1997) and incubated at the non-permissive temperature of 40° C. for 20 hr. To initiate exit of ts045-VSVG-GFP from the ER, cells were shifted to 32° C. for 15 min, 45 min, and 90 min and fixed. Cells were examined by fluorescence microscopy to determine the subcellular localization of ts045-VSVG-GFP. Vesicles containing ts045-VSVG-GFP were generated from cells shifted to the permissive temperature for either 15 min or 90 min using the protocol described for RFP-KDEL vesicles. Epifluorescence microscopy and TIRF microscopy studies to visualize the binding and fusion of ts045-VSVG-GFP to the PM of semi-intact cells was conducted as described above for RFP-KDEL-containing vesicles.
Highlights
  • The L. pneumophila effector DrrA activates Rab1 and binds plasma membrane (PM) syntaxins
  • PM-localized Rab1 GTPase stimulates ER-derived vesicle tethering
  • SNARE protein Sec22b and PM-localized syntaxins promote ER-derived vesicle fusion
  • DrrA promotes remodeling of PM-derived organelles by ER-derived vesicles
Supplementary Material
01
Acknowledgments
We wish to thank S. Shin, S. Mukherjee, S. Ivanov, and A. Hubber for their helpful discussions and assistance in manuscript preparation, M. Tagaya for experimental support, and B. Lindenbach for technical advice and for providing the plasmid encoding Luciferase-KDEL. This work was supported by NIH grants R01AI041699 (CRR) and DP2-OD002980-01 (DT).
Footnotes
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