PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of narLink to Publisher's site
 
Nucleic Acids Res. Dec 20, 1983; 11(24): 8861–8876.
PMCID: PMC326630
Interaction of diamidino-2-phenylindole (DAPI) with natural and synthetic nucleic acids.
G Manzini, M L Barcellona, M Avitabile, and F Quadrifoglio
Abstract
The interaction of DAPI with natural and synthetic polydeoxynucleotides of different base content and sequences was studied with circular dichroism, ultracentrifugation, viscosity and calorimetry. All the polymers show two types of binding. The strength of the interaction and its resistance to ionic strength are related to the content of AT clusters in the chain. On the other hand, sedimentation measurements rule out an intercalation mechanism. A model of DAPI interaction with DNA, similar to that displayed by distamycin and netropsin, is proposed.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page.
Articles from Nucleic Acids Research are provided here courtesy of
Oxford University Press