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Nucleic Acids Res. 1983 March 25; 11(6): 1747–1757.
PMCID: PMC325833

The complete nucleotide sequences of the cloned hepatitis B virus DNA; subtype adr and adw.

Abstract

The complete nucleotide sequences of two different subtypes (adr and adw) of hepatitis B virus (HBV) DNA cloned in E. coli were determined. The sequence of the viral genome of the adr clone was 3188 nucleotides long, and that of the adw clone was 3200 nucleotides long. The adr and adw clones differed from the reported cloned ayw HBV DNA (3182 nucleotides long) in 11.2% and 10.0% of nucleotides, respectively. Heterogeneity of the HBV genome in the clones with the same subtype was observed.

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Selected References

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  • Robinson WS, Greenman RL. DNA polymerase in the core of the human hepatitis B virus candidate. J Virol. 1974 Jun;13(6):1231–1236. [PMC free article] [PubMed]
  • Hruska JF, Robinson WS. The proteins of hepatitis B Dane particle cores. J Med Virol. 1977;1(2):119–131. [PubMed]
  • Le Bouvier GL. Editorial: Subtypes of hepatitis B antigen: clinical relevance? Ann Intern Med. 1973 Dec;79(6):894–896. [PubMed]
  • Siddiqui A, Sattler F, Robinson WS. Restriction endonuclease cleavage map and location of unique features of the DNA of hepatitis B virus, subtype adw2. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4664–4668. [PubMed]
  • Sninsky JJ, Siddiqui A, Robinson WS, Cohen SN. Cloning and endonuclease mapping of the hepatitis B viral genome. Nature. 1979 May 24;279(5711):346–348. [PubMed]
  • Charnay P, Mandart E, Hampe A, Fitoussi F, Tiollais P, Galibert F. Localization on the viral genome and nucleotide sequence of the gene coding for the two major polypeptides of the hepatitis B surface antigen (HBs Ag). Nucleic Acids Res. 1979 Sep 25;7(2):335–346. [PMC free article] [PubMed]
  • Pasek M, Goto T, Gilbert W, Zink B, Schaller H, MacKay P, Leadbetter G, Murray K. Hepatitis B virus genes and their expression in E. coli. Nature. 1979 Dec 6;282(5739):575–579. [PubMed]
  • Galibert F, Mandart E, Fitoussi F, Tiollais P, Charnay P. Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli. Nature. 1979 Oct 25;281(5733):646–650. [PubMed]
  • Valenzuela P, Gray P, Quiroga M, Zaldivar J, Goodman HM, Rutter WJ. Nucleotide sequence of the gene coding for the major protein of hepatitis B virus surface antigen. Nature. 1979 Aug 30;280(5725):815–819. [PubMed]
  • Yamishita Y, Kurashina S, Miyakawa Y, Mayumi M. South-to-north gradient in distribution of the r determinant of hepatitis B surface antigen in Japan. J Infect Dis. 1975 May;131(5):567–569. [PubMed]
  • Nishioka K, Levin AG, Simons MJ. Hepatitis B antigen, antigen subtypes, and hepatitis B antibody in normal subjects and patients with liver disease. Bull World Health Organ. 1975;52(3):293–300. [PubMed]
  • Onda H, Matsuda S, Oka Y. Hepatitis B core particles with endogenous DNA polymerase activity from chimpanzee liver. Intervirology. 1978;10(4):254–264. [PubMed]
  • Kaplan PM, Greenman RL, Gerin JL, Purcell RH, Robinson WS. DNA polymerase associated with human hepatitis B antigen. J Virol. 1973 Nov;12(5):995–1005. [PMC free article] [PubMed]
  • Mason WS, Seal G, Summers J. Virus of Pekin ducks with structural and biological relatedness to human hepatitis B virus. J Virol. 1980 Dec;36(3):829–836. [PMC free article] [PubMed]
  • Enea V, Vovis GF, Zinder ND. Genetic studies with heteroduplex DNA of bacteriophage fl. Asymmetric segregation, base correction and implications for the mechanism of genetic recombination. J Mol Biol. 1975 Aug 15;96(3):495–509. [PubMed]
  • Ish-Horowicz D, Burke JF. Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981 Jul 10;9(13):2989–2998. [PMC free article] [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PubMed]
  • Messing J, Crea R, Seeburg PH. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. [PMC free article] [PubMed]
  • Peterson DL, Nath N, Gavilanes F. Structure of hepatitis B surface antigen. Correlation of subtype with amino acid sequence and location of the carbohydrate moiety. J Biol Chem. 1982 Sep 10;257(17):10414–10420. [PubMed]
  • Charnay P, Pourcel C, Louise A, Fritsch A, Tiollais P. Cloning in Escherichia coli and physical structure of hepatitis B virion DNA. Proc Natl Acad Sci U S A. 1979 May;76(5):2222–2226. [PubMed]
  • Chakraborty PR, Ruiz-Opazo N, Shouval D, Shafritz DA. Identification of integrated hepatitis B virus DNA and expression of viral RNA in an HBsAg-producing human hepatocellular carcinoma cell line. Nature. 1980 Jul 31;286(5772):531–533. [PubMed]
  • Valenzuela P, Medina A, Rutter WJ, Ammerer G, Hall BD. Synthesis and assembly of hepatitis B virus surface antigen particles in yeast. Nature. 1982 Jul 22;298(5872):347–350. [PubMed]
  • Edman JC, Hallewell RA, Valenzuela P, Goodman HM, Rutter WJ. Synthesis of hepatitis B surface and core antigens in E. coli. Nature. 1981 Jun 11;291(5815):503–506. [PubMed]
  • Charnay P, Gervais M, Louise A, Galibert F, Tiollais P. Biosynthesis of hepatitis B virus surface antigen in Escherichia coli. Nature. 1980 Aug 28;286(5776):893–895. [PubMed]
  • Pourcel C, Louise A, Gervais M, Chenciner N, Dubois MF, Tiollais P. Transcription of the hepatitis B surface antigen gene in mouse cells transformed with cloned viral DNA. J Virol. 1982 Apr;42(1):100–105. [PMC free article] [PubMed]
  • Benoist C, Chambon P. In vivo sequence requirements of the SV40 early promotor region. Nature. 1981 Mar 26;290(5804):304–310. [PubMed]
  • Cohen BJ, Richmond JE. Electron microscopy of hepatitis B core antigen synthesized in E. coli. Nature. 1982 Apr 15;296(5858):677–679. [PubMed]
  • Tiollais P, Charnay P, Vyas GN. Biology of hepatitis B virus. Science. 1981 Jul 24;213(4506):406–411. [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press