PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

Logo of jcellbiolHomeThe Rockefeller University PressThis articleEditorsContactInstructions for AuthorsThis issue
 

Figure 3.

An external file that holds a picture, illustration, etc.
Object name is JCB_201105098_GS_Fig3.jpg

The presequence-binding domain of Tim50 is essential. (A) Diploid yeast cells carrying a single chromosomal deletion of TIM50366-476 were sporulated and subjected to tetrad dissection. (B) Yeast cells containing a chromosomal deletion of TIM50, complemented by a TIM50-containing plasmid carrying URA3, were transformed with plasmid constructs encoding the indicated Tim50 versions and subjected to plasmid loss on 5-FOA–containing medium. (C) Western blot analysis of mitochondria isolated from the cells described in A with the indicated antibodies. (D) In organello photo cross-linking of presequence probes using mitochondria from indicated strains for 30 min. Samples were analyzed as in C.

Images in this article

  • Figure 1.
  • Figure 2.
  • Figure 3.
  • Figure 4.
  • Figure 5.
  • Figure 6.
Click on the image to see a larger version.