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J Bacteriol. 2012 January; 194(2): 545–546.
PMCID: PMC3256645

Whole-Genome Sequences of Borrelia bissettii, Borrelia valaisiana, and Borrelia spielmanii

Abstract

It has been known for decades that human Lyme disease is caused by the three spirochete species Borrelia burgdorferi, Borrelia afzelii, and Borrelia garinii. Recently, Borrelia valaisiana, Borrelia spielmanii, and Borrelia bissettii have been associated with Lyme disease. We report the complete genome sequences of B. valaisiana VS116, B. spielmanii A14S, and B. bissettii DN127.

GENOME ANNOUNCEMENT

The bacteria that cause human Lyme disease belong to a group of at least 15 species, referred to as Borrelia burgdorferi sensu lato, or the Lyme disease agent bacterial group (20). Among these, B. burgdorferi sensu stricto causes Lyme disease in North America, while in Europe and eastern Asia Borrelia afzelii, Borrelia garinii, and Borrelia bavariensis sp. nov. are the best-known causes (reference 15 and references therein); however, more recently, Borrelia bissettii, Borrelia lusitaniae, Borrelia spielmanii, and Borrelia valaisiana have been isolated from Lyme disease patients (58, 16, 17). Other species in this bacterial group, such as Borrelia japonica and Borrelia sincia in Asia, have not been associated with human disease. To date, genome sequences have been reported for 14 B. burgdorferi isolates (1, 9, 19), 2 B. afzelii isolates (4, 11), 2 B. garinii isolates (4, 11), 1 B. bavariensis sp. nov. isolate (10), and 1 isolate of unassigned species (2).

We report here the complete genome sequences for three additional Borrelia species: B. valaisiana isolate VS116 (from an Ixodes rinicus tick [Switzerland]) (14, 18), B. bissettii isolate DN127 clone 9 (Ixodes pacificus tick [northern California]) (12), and B. spielmanii isolate A14S (human skin [The Netherlands]) (21). DNA samples from low-passage isolates were sequenced to minimize plasmid loss, and genomes were sequenced to about 8-fold coverage as previously described (13). Genome annotation was performed using the JCVI Prokaryotic Annotation Pipeline (www.jcvi.org/cms/research/projects/annotation-service/overview/). The DN127 chromosome and 35 of 39 plasmid sequence contigs were closed, but in order to maximize the use of available funds, the sequences of a few replicons were not closed and some gaps remained in these sequences (two chromosomes and one cp9 and three cp32 plasmids, because they are much less variable than the other plasmids).

These three genome sequences include 3,914,891 bp in total (1,258,865, 1,403,466, and 1,252,560 bp for strains VS116, DN127, and A14S, respectively), with an average of 1,304,497 bp/genome. Like the sequences of other Borrelia species, they include numerous linear plasmids (6, 7, and 7, respectively) and circular plasmids (2, 2, and 2, respectively). Plasmid numbers in these three strains range from 11 in VS116 and 12 in A14S to 16 in DN127. Plasmids that are very similar to B. burgdorferi sensu stricto cp26, cp32 (7 in DN127, versus 3 in the other two strains analyzed), and lp54 plasmids are present in each of these isolates, and DN127 also contains an unusual fusion of four partial cp32 plasmids. Plasmids with predicted lp17 compatibility (3) are also present in all three genomes, making it the only other plasmid type, in addition to cp26 and lp54, to be found in all 23 B. burgdorferi sensu lato sequenced genomes. However, the gene contents of the lp17s are much more variable than the other universally present plasmids.

The detailed analyses of these genome sequences will be a major step forward in attaining a complete understanding of B. burgdorferi sensu lato diversity. They will contribute to the development of species- and group-specific vaccines and diagnostic tools, as well as inform us whether these species are in genetic contact with the more-common Lyme disease-associated agents. These foundational sequencing efforts can now be further developed with the use of evolving deep sequencing methods.

Nucleotide sequence accession numbers.

The nucleotide sequences for these three strains and relevant replicons have been deposited in the GenBank database, and their accession numbers are listed in Table 1.

Table 1
B. valaisiana, B. bissettii, and B. spielmanii sequence accession numbers

ACKNOWLEDGMENTS

This research was supported by grants from the National Institutes of Health: AI49003, AI37256 N01-AI30071, GM083722, and RR03037.

We thank P. Rosa for strain DN127.

REFERENCES

1. Casjens S, et al. 2000. A bacterial genome in flux: the twelve linear and nine circular extrachromosomal DNAs in an infectious isolate of the Lyme disease spirochete Borrelia burgdorferi. Mol. Microbiol. 35:490–516. [PubMed]
2. Casjens SR, et al. 2011. Whole genome sequence of an unusual Borrelia burgdorferi sensu lato isolate. J. Bacteriol. 193:1489–1490. [PMC free article] [PubMed]
3. Casjens SR, et al. 2006. Comparative genomics of Borrelia burgdorferi, p. 79–95 . In Cabello FC, Hulinska D, Godfrey HP, editors. (ed.), Molecular biology of spirochetes. IOS Press, Amsterdam, Netherlands:
4. Casjens SR, et al. 2011. Whole-genome sequences of two Borrelia afzelii and two Borrelia garinii Lyme disease agent isolates. J. Bacteriol. 193:6995–6996. [PMC free article] [PubMed]
5. Collares-Pereira M, et al. 2004. First isolation of Borrelia lusitaniae from a human patient. J. Clin. Microbiol. 42:1316–1318. [PMC free article] [PubMed]
6. de Carvalho IL, et al. 2008. Vasculitis-like syndrome associated with Borrelia lusitaniae infection. Clin. Rheumatol. 27:1587–1591. [PubMed]
7. Diza E, et al. 2004. Borrelia valaisiana in cerebrospinal fluid. Emerg. Infect. Dis. 10:1692–1693. [PMC free article] [PubMed]
8. Fingerle V, et al. 2007. Epidemiological aspects and molecular characterization of Borrelia burgdorferi s. l. from southern Germany with special respect to the new species Borrelia spielmanii sp. nov. Int. J. Med. Microbiol. 298:279–290. [PubMed]
9. Fraser CM, et al. 1997. Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi. Nature 390:580–586. [PubMed]
10. Glöckner G, et al. 2004. Comparative analysis of the Borrelia garinii genome. Nucleic Acids Res. 32:6038–6046. [PMC free article] [PubMed]
11. Glöckner G, et al. 2006. Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity. BMC Genomics 7:211. [PMC free article] [PubMed]
12. Kurashige S, Bissett M, Oshiro L. 1990. Characterization of a tick isolate of Borrelia burgdorferi that possesses a major low-molecular-weight surface protein. J. Clin. Microbiol. 28:1362–1366. [PMC free article] [PubMed]
13. Nelson KE, et al. 2004. Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species. Nucleic Acids Res. 32:2386–2395. [PMC free article] [PubMed]
14. Peter O, Bretz AG. 1992. Polymorphism of outer surface proteins of Borrelia burgdorferi as a tool for classification. Zentralbl. Bakteriol. 277:28–33. [PubMed]
15. Piesman J, Gern L. 2004. Lyme borreliosis in Europe and North America. Parasitology 129(Suppl.):S191–S220. [PubMed]
16. Rijpkema SG, et al. 1997. Detection of Borrelia afzelii, Borrelia burgdorferi sensu stricto, Borrelia garinii and group VS116 by PCR in skin biopsies of patients with erythema migrans and acrodermatitis chronica atrophicans. Clin. Microbiol. Infect. 3:109–116. [PubMed]
17. Rudenko N, et al. 2009. Molecular detection of Borrelia bissettii DNA in serum samples from patients in the Czech Republic with suspected borreliosis. FEMS Microbiol. Lett. 292:274–281. [PubMed]
18. Ryffel K, Peter O, Dayer E, Bretz AG, Godfroid E. 2003. OspA heterogeneity of Borrelia valaisiana confirmed by phenotypic and genotypic analyses. BMC Infect. Dis. 3:14. [PMC free article] [PubMed]
19. Schutzer SE, et al. 2011. Whole-genome sequences of thirteen isolates of Borrelia burgdorferi. J. Bacteriol. 193:1018–1020. [PMC free article] [PubMed]
20. Stanek G, Reiter M. 2011. The expanding Lyme Borrelia complex: clinical significance of genomic species? Clin. Microbiol. Infect. 17:487–493. [PubMed]
21. Wang G, van Dam AP, Dankert J. 1999. Phenotypic and genetic characterization of a novel Borrelia burgdorferi sensu lato isolate from a patient with lyme borreliosis. J. Clin. Microbiol. 37:3025–3028. [PMC free article] [PubMed]

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