Herbal medicines are the oldest remedies known to mankind; these generally contain more than one herb in the combination. Although, considerable work is being done to evaluate herbals for their quality, safety and efficacy, still a need of, a well-defined specific method for routine analysis of herbal raw materials and formulations with regard to constituents that may be responsible for efficacy is generally felt. Development of methods for analysis of plant products poses difficulty, due to their unknown chemical profile, more so in case of multi components herbal formulations.
In the present study, an attempt has been made to develop a simple, rapid and accurate HPTLC method for simultaneous estimation of glycyrrhetinic acid and piperine in a marketed herbomineral capsule formulation (Efiplus® Capsules) indicated for use in iron deficient anaemia.
Glycyrrhetinic acid is one of the main active constituent of roots of Glycyrrhiza glabra
. It is a recognized medicine in India as an expectorant traditionally and used in various preparations affecting gastrointestinal system. The herb also contains glycyrrhizin, flavonoids, isoflavonoids, and chalcones as major active chemical constituents[1
]. The herb is reported with the following studies antiulcer & antioxidant, antioxidant and wound healing, anxiolytic, carminative and antiemetic, antifungal, nephroprotective, antimicrobial, immuno-modulatory, antiinflaunmatory, antiasthmatic[2
]. Pippali (Piper longum
) is a powerful stimulant for both the digestive and the respiratory systems[4
]. Pippali a typical ayurvedic complementary component whose benefit is to increase the bioavailability and enhance absorption of the other active ingredients[5
]. It contains mainly alkaloids (piperine as major alkaloid) and amides, lignins, esters, volatile oils. The whole plant is considered by tribal people in India to be useful in splenic disorders, cholera, dysentery, asthma, cough and bronchitis[7
]. It is studied for various biological activities viz. immunomodulatory activity, stimulant activity, antiasthmatic activity, hepatoprotective activity, hypocholesterolaemic activity, antiinflammatory activity[9
These two herbs are most common components of ayurvedic medicine and herbal medicines used for treating gastrointestinal, respiratory system and metabolic disorders. Some of the marketed ayurvedic medicines that contain these two herbs are Khadirari shta, Lohasava, Ashvagandharishta, Dasmularishta, Chandanasava, Satvaryadi ghrita etc. Various herbal medicines used as antacid, hepatoprotective, antiulcer, dyspeptic, haematinic preparations etc are also known to contain these two herbs e.g. Deepana capsule, Dicolai capsule, Suryacid tablet, Diobliv, panchasav, efiplus caps, neotab etc.