PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

Logo of acbAnatomy & Cell BiologyAboutFor Contributorse-SubmissionThis article
 
From:
Published online 2011 December 30. doi: 10.5115/acb.2011.44.4.265

Fig. 2

An external file that holds a picture, illustration, etc.
Object name is acb-44-265-g002.jpg

L-flow inhibits 15d-PGJ2-induced endothelial apoptosis via p53 down regulation. (A) HUVECs were exposed to L-flow followed by treatment with 15d-PGJ2 for 8 h. Protein expression was determined by Western blotting using anti-p53 and anti-tubulin antibodies. (B) HUVECs were transfected with either control or p53 siRNA (si-p53) for two days. Cells were then exposed to 10 µM 15d-PGJ2 for 8 h. The expression of cleaved caspase 3, p53, and tubulin was detected by Western blotting with the respective antibodies. (C) Cell viability was determined by MTT assay. Data are expressed as the mean±SD from three independent experiments. *P<0.01. L-flow, steady laminar flow; 15d-PGJ2, 15-deoxy-Δ(12,14)-prostaglandin J2; HUVECs, human umbilical vein endothelial cells.

Images in this article

  • Fig. 1
  • Fig. 2
  • Fig. 3
  • Fig. 4
Click on the image to see a larger version.