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Fas (also called CD95 or APO-1), a member of a subgroup of the tumour necrosis factor receptor superfamily that contain an intracellular death domain, can initiate apoptosis signalling and has a critical role in the regulation of the immune system. Fas-induced apoptosis requires recruitment and activation of the initiator caspase, caspase-8 (in humans also caspase-10), within the death-inducing signalling complex. In so-called type 1 cells, proteolytic activation of effector caspases (-3 and -7) by caspase-8 suffices for efficient apoptosis induction. In so-called type 2 cells, however, killing requires amplification of the caspase cascade. This can be achieved through caspase-8-mediated proteolytic activation of the pro-apoptotic Bcl-2 homology domain (BH)3-only protein BH3-interacting domain death agonist (Bid), which then causes mitochondrial outer membrane permeabilisation. This in turn leads to mitochondrial release of apoptogenic proteins, such as cytochrome c and, pertinent for Fas death receptor (DR)-induced apoptosis, Smac/DIABLO (second mitochondria-derived activator of caspase/direct IAP binding protein with low Pi), an antagonist of X-linked inhibitor of apoptosis (XIAP), which imposes a brake on effector caspases. In this review, written in honour of Juerg Tschopp who contributed so much to research on cell death and immunology, we discuss the functions of Bid and XIAP in the control of Fas DR-induced apoptosis signalling, and we speculate on how this knowledge could be exploited to develop novel regimes for treatment of cancer.
Fas (also called CD95 or APO-1) is a member of the tumour necrosis factor receptor (TNF-R) superfamily, which also includes receptors for TNFα, TRAIL (TNF-related apoptosis-inducing ligand), receptor activator of NF-κB ligand (RANKL), CD40 ligand (CD40L) and other members of the TNF family of cytokines.1 Specifically, Fas belongs to the subgroup of TNF-R family members that have an intracellular death domain; this subgroup also includes TNF-R1, DR3 and the two TRAIL receptors, DR4 and DR5.1 The first and hereto best-characterised function of Fas is its ability to induce apoptosis in a large number of cell types.2 Besides apoptosis, a fundamental form of physiologically relevant programmed cell death in multicellular organisms, Fas and other so-called death receptors (DRs), have recently also been implicated in the activation of the kinase RIPK1, resulting in a cell death with necrotic characteristics, termed necroptosis.3, 4, 5, 6 Even though Fas, a trimeric plasma membrane-bound surface receptor, is expressed on most cell types throughout the body, it is of particular importance in the control of the immune system, where it functions as a guardian against autoimmunity and tumourigenesis.2 Although Fas and other DRs are clearly able to kill cells, it has become evident over the last decade that in addition, Fas can also activate several other, non-death-related signalling pathways, leading to differentiation or proliferation,7 a concept to which Juerg Tschopp has made seminal contributions (see Box 1).
During his great career, Juerg Tschopp has shown great interest in the function, mechanisms of activation, and regulation of cellular proteases that are central for cell death and pro-inflammatory signalling pathways. In addition to his earlier groundbreaking work on perforin/granzymes, and his more recent discoveries and extraordinary contributions in the field of the inflammasomes (see other reviews in this commemorative issue), Juerg Tschopp was also among the pioneers to understand the molecular mechanisms of death-receptor signalling. His work covered several members of the TNF receptor superfamily, and some of his major contributions are listed below:
In contrast to the broad tissue distribution of Fas, the expression of the physiological ligand for Fas, FasL (CD95L), is restricted to a highly selective cellular pool, including activated-T cells, NKT cells and NK cells.1, 8 Expression of FasL on certain non-lymphoid tissues, such as the eye and testis, has also been reported, but this is controversial.1, 9 Cells express FasL either in a membrane-bound form on their surface or, through the action of certain metalloproteases, such as ADAM10,10 FasL can be cleaved and released in a soluble trimeric form. After extended controversies, studies with fasl mutant mice generated by gene-targeting in ES cells, which can express either only membrane-bound FasL or only secreted FasL, have demonstrated beyond doubt that only the membrane-bound form of FasL is capable of inducing apoptosis,11 a result that had been predicted by Juerg Tschopp on the basis of his laboratory's studies with recombinant forms of FasL. Biochemical studies have shown that for Fas to elicit apoptosis, there needs to be aggregation of multiple pre-assembled Fas trimers on the surface of responding cells.12 This can of course be achieved much more readily by membrane-bound FasL than by soluble FasL trimers. Although soluble trimeric FasL fails to trigger cell death, there is considerable evidence that it is involved in Fas-induced activation of NF-κB and MAP kinases, and consequent initiation of signalling pathways that promote cell survival or processes unrelated to apoptosis, such as cell differentiation or inflammation. These functions of soluble FasL were first demonstrated by Juerg Tschopp and his colleagues,13 in experiments using recombinant ligand to stimulate tumour-derived cell lines or primary cells. Remarkably, knock-in mutant mice that lack membrane-bound FasL, but express secreted FasL, which is able to bind to its receptor Fas, succumb to diseases, including SLE-like glomerulonephritis, autoimmune dermatitis and histiocytic sarcoma, considerably more rapidly than gld mutant mice that have a point mutation in FasL that prevents binding to Fas, and therefore, abolishes the function of both membrane-bound, as well as soluble FasL.11 This indicates that when membrane-bound FasL/Fas-induced apoptosis is blocked, soluble FasL/Fas-induced activation of non-apoptotic pathways can enhance development of autoimmune disease and cancer, possibly by eliciting inflammatory responses.
The molecular events that are activated by FasL to trigger Fas-mediated apoptosis are well characterised (Figure 1).2 Membrane FasL leads to aggregation of pre-assembled Fas trimers on the plasma membrane of target cells.12, 14, 15, 16, 17 Aggregation-induced conformational changes in Fas lead to the formation of the death-inducing signalling complex (DISC),18 which involves recruitment of the adaptor protein FADD/Mort1 through a homotypic interaction of death domains, present in both the intracellular region of FAS and the C-terminus of FADD.19, 20 Through a second homotypic interaction, involving death-effector domains present in the N-termini of both FADD and pro-caspase-8, the initiator caspase (as well as procaspase-10 in humans) is recruited into the DISC.19, 21 Caspase-8 is an aspartate-specific cysteine protease, which is present in healthy cells as an inactive proenzyme (zymogen). Through complex proximity-driven conformational changes, full-length procaspase-8 molecules within the DISC gain full enzymatic activity, resulting in the auto-proteolytic processing of caspase-8 into large (~p20) and small (~p10) subunits, and assembly of a hetero-tetrameric enzyme (p202p102).22 This active, processed caspase-8 can leave the DISC to target its specific substrates within the cytosol, such as effector caspases (caspase-3, caspase-7) and the pro-apoptotic BH3-only protein Bid, which are all critical for Fas-mediated apoptosis.23, 24, 25, 26, 27 Work by Juerg Tschopp and others has shown that caspase-8 activation within the DISC can be regulated positively or negatively by cellular FLICE-inhibitory protein (c-FLIP), a protein structurally resembling caspase-8, but lacking a catalytic site.28 Low concentrations of c-FLIPL promote caspase-8 recruitment and activation, whereas high concentrations of c-FLIPL inhibit capase-8 activation, likely due to competition for FADD interaction.22 Isoforms of c-FLIP lacking its caspase-like domain (c-FLIPs and c-FLIPR, the only short c-FLIP isoform expressed in the mouse,29), as well as viral forms of FLIP act solely as potent inhibitors of caspase-8 recruitment and activation.29, 30, 31
Members of the Bcl-2 protein family have a major function in regulating apoptosis in a broad range of species, ranging from nematodes to man.32 This protein family regulates an apoptotic pathway that is largely distinct from the DR-induced apoptosis signalling pathway,33 although the two pathways converge at the level of effector caspase activation.34 Bcl-2 family members are highly conserved throughout evolution, with amino acid sequence homology concentrated within regions termed BH domains. On the basis of the presence of the BH domains and function, three major subgroups can be distinguished within the Bcl-2 family. Bcl-2, Bcl-xL, Mcl-1, A1 (BFL1) and Bcl-w share up to four BH domains (BH1–BH4), and these proteins are critical for cell survival, exerting cell-type restricted, as well as overlapping anti-apoptotic functions.32 The Bax/Bak-like proteins (possibly also including Bok) share remarkable structural similarity with their pro-survival relatives, but they are instead required for activation of the downstream (effector) stages of cellular demolition in apoptosis signalling.35, 36 Upon activation, Bax/Bak mediate the mitochondrial outer membrane permeabilisation (MOMP), with consequent release of apoptogenic factors from mitochondria into the cytosol (cytochrome c, Smac/DIABLO (second mitochondria-derived activator of caspase/direct IAP binding protein with low pI)). These apoptogenic factors precipitate activation of the caspase cascade and cell-killing via Apaf-1-mediated activation of the initiator caspase, caspase-9, as well as by derepression of effector caspases by blocking their antagonist XIAP.35, 36 The third subgroup of the Bcl-2 family consists of the so-called BH3-only proteins (Bad, Bik/Blk/Nbk, Hrk/DP5, Bid, Bim/Bod, Bmf, Noxa and Puma/Bbc3). These proteins share with each other, and the wider Bcl-2 protein family, only the BH3 domain, which is required for their pro-apoptotic activity. BH3-only proteins are essential for initiation of apoptosis signalling, being activated transcriptionally and/or post-transcriptionally in a cell death stimulus-specific (and sometimes also cell type-specific) manner.32, 37 BH3-only proteins differ in their specificity for binding pro-survival Bcl-2 family members: Bim, Puma and activated Bid, called tBid (so-called promiscuous binders), bind with high affinity to all pro-survival Bcl-2-like proteins, whereas Bad binds only to Bcl-2, Bcl-xL and Bcl-w, but not Mcl-1 or A1 and, conversely, Noxa only binds to Mcl-1 and A1.38, 39 Accordingly, Bim, Puma and tBid are potent killers, but efficient cell killing by Bad can only be achieved when combined with Noxa or knockdown of Mcl-1/A1.38, 39 It is firmly established that BH3-only proteins are essential for initiation of apoptosis signalling (e.g. see Bouillet et al.40 and Villunger et al.41) and that they require Bax/Bak for cell killing,42, 43 but the biochemical mechanisms by which BH3-only proteins activate Bax/Bak remain unclear (Figure 2). According to the direct model, the so-called activator BH3-only proteins, including Bim, tBid and possibly Puma, can directly bind to and activate Bax/Bak, whereas the so-called sensitiser BH3-only proteins (e.g. Bad, Noxa, Bik) act by binding to pro-survival Bcl-2-like proteins, thereby causing release of the activator BH3-only proteins.35, 44 Conversely, the indirect model proposes that, in healthy cells, Bax/Bak are kept in check by the pro-survival Bcl-2 family members and also proposes that for apoptotic death to occur, all pro-survival Bcl-2-like proteins present within a given cell need to be neutralised by BH3-only proteins, thereby derepressing Bax/Bak.45
BH3-only proteins are tightly regulated at the transcriptional, as well as post-translational level.46 For example, puma47, 48 and noxa49 gene expression is directly upregulated by the transcription factor p53, a tumour suppressor that is disabled by mutation in ~50% of all human cancers.50 Experiments with gene-targeted mice have identified the critical individual, as well as overlapping functions of BH3-only proteins.51 For example, Bim is essential for apoptosis induced by growth factor withdrawal, deregulated calcium flux40 and ER stress,52 and is critical for the developmentally programmed death of autoreactive T cells53 and B cells54 during their development in the thymus or bone marrow, respectively. Conversely, Puma and, to a lesser extent, Noxa are required for DNA damage-induced, p53-mediated apoptosis.41, 55, 56, 57
Bid, unlike all other known BH3-only proteins, needs to be proteolysed by caspase-8 or other proteases to attain potent pro-apoptotic activity.23, 24 Once cleaved, tBid translocates to the mitochondrial outer membrane, facilitated by its interaction with MTCH2/MIMP,58 where it leads to the activation of Bax/Bak and consequently MOMP, caspase activation and cell demolition. Processing of Bid into tBid is mainly mediated by caspase-8, the apical caspase in the DR apoptotic pathway.23, 24 Activation of Bid, therefore, constitutes a process for crosstalk between the DR and the Bcl-2-regulated apoptotic pathways, allowing amplification of the caspase cascade to ensure efficient cell demolition (Figure 1). Experiments with diverse cell types from bid−/− mice have shown that Bid is critical for Fas-induced apoptosis in certain cell types (so-called type 2 cells), including hepatocytes and pancreatic β-cells, but dispensable in others (so-called type 1 cells), such as thymocytes or mature T cells.59, 60, 61 This indicates that amplification of the caspase cascade is critical for Fas-induced apoptosis in the former, but not the latter cell types.
The tBid-mediated crosstalk to the Bcl-2-regulated apoptotic pathway amplifies the pro-apoptotic signal through activation of Bax/Bak, consequent induction of MOMP with release of apoptogenic factors, including cytochrome c and the IAP antagonists (Smac/DIABLO, Omi/Htra2), ultimately causing cell demolition by the enhanced caspase cascade. The precise contribution of mitochondrial factors to Fas-induced apoptosis is still not fully understood. Remarkably, a mutation in cytochrome c that interferes with its interaction with the caspase-9 adaptor Apaf-1 and therefore blocks apoptosome formation provided no protection against Fas-induced hepatocyte killing and consequent fatal hepatitis.62 Conversely, overexpression of anti-apoptotic Bcl-2 family members63 or combined loss of Bax/Bak64, 65 conferred (at least partial) protection from Fas-induced apoptosis in type 2 cells (e.g. hepatocytes), but not in type 1-like cells (e.g. thymocytes).33, 66 This indicates that activation of Bax/Bak constitutes the point of no return in Fas-induced killing of type 2 cells that is dependent on Bid-mediated amplification via the Bcl-2-regulated apoptotic pathway, whereas caspase-9 activity is not required. Thus, disruption of mitochondrial outer membrane integrity per se or mitochondrial release of antagonists of XIAP (e.g. Smac/DIABLO) that de-repress effector caspases must be critical for Fas-induced killing of type 2 cells.
Interestingly, it is possible to override the need for the mitochondrial apoptotic pathway for Fas-mediated killing of type 2 cells by using a very strong Fas-agonist, such as a hexameric form of FasL (called MegaFasL, developed by Juerg Tschopp's laboratory).67 For example, in mice deficient for Bid, an injection of MegaFasL induced killing of parenchymal and possibly non-parenchymal cells within the liver, thereby driving hepatocellular demise and death of experimental animals, although the fatal outcome was still clearly delayed compared with wild-type mice.67 This finding demonstrates that high level caspase-8-mediated activation of effector caspases, achieved by extensive Fas crosslinking on the cell surface, can bypass the need for the Bid>Bax/Bak>MOMP amplification loop in type 2 cells, and this is consistent with the notion that XIAP acting as an inhibitor of effector caspases can regulate this process (see below).
Experiments with bid−/− mice have shown that caspase-8-mediated proteolytic activation of Bid is also critical for hepatocyte killing and fatal hepatitis mediated by TNF>TNF-R1 stimulation, for example, elicited by injection with LPS plus the hepatocyte-specific sensitiser GalN (to block anti-apoptotic signalling from TNF-R1, which requires de novo gene transcription).68, 69 In this pathological scenario, but not in FasL-induced liver destruction, tBid cooperates with the BH3-only protein Bim70, which is activated by TNF-R1 in a caspase-8-independent manner, to kill hepatocytes.69
Proteases other than caspase-8 have been reported to cleave and convert Bid into tBid (or similarly active truncated forms of Bid); they include granzyme B,71 lysosomal cathepsins72 and calpain,73, 74 although unlike for caspase-8-mediated cleavage of Bid in Fas-, DR-4/-5- or TNF-R1-induced apoptosis, the physiological relevance of these processes for Bid proteolysis are presently still unclear. The functions of full-length Bid are only poorly understood, but there are reports showing a pro-apoptotic activity of full-length Bid on mitochondria.75, 76 Moreover, full-length Bid has very recently been reported to be critical for a non-cell death-related process, namely the production of pro-inflammatory cytokines.77
XIAP can inhibit apoptosis by binding and thereby inactivating certain caspases, including the initiator caspase-9 and the effector caspases (-3 and -7).78, 79 XIAP stands out of the mammalian IAP protein family, because it is the only member capable of blocking active caspases. The binding affinity of XIAP to caspases (-3 and -7) is very strong, whereas binding to the initiator caspase-9 is of considerably lower affinity and binding to the initiator caspase-8 is undetectable.80 This affinity profile places XIAP as an inhibitor of the common effector phase of apoptosis, hence acting downstream of the Fas death-receptor stimulation and the receptor proximal signalling events within the DISC, including FADD-mediated recruitment and activation of caspase-8 (Figure 1). XIAP contains three baculovirus internal repeat (BIR) domains that make up most of the amino terminal portion of the protein. Interestingly, the BIR domains are functionally distinct. BIR1 facilitates binding of XIAP to the TGFβ-activated kinase binding protein 1 (TAB1), thereby mediating activation of NF-κB.81 In contrast, the BIR2/BIR3 domains mediate binding of XIAP to proteins harbouring an IAP-binding motif, including active caspases. This interaction is facilitated by a deep hydrophobic peptide-binding groove within the BIR2 and BIR3.80, 82, 83, 84 In addition, the linker region located adjacent to the BIR2 domain also participates in the binding of XIAP to the effector caspases (-3 and -7) by inserting a steric hindrance into the active site pocket of these proteases.85, 86
The function of XIAP in death-receptor signalling has classically been associated with the presence of BIR domains and the direct inhibitory interaction with effector caspases.87 However, more recently its has been revealed that XIAP possesses E3 ligase activity and that, in response to ligation of at least certain DRs, XIAP is capable of targeting some signalling proteins for ubiquitylation.88 These non-BIR domain-mediated functions of XIAP have proven to be more complex than initially anticipated, and we still do not fully understand which signalling pathways are regulated by XIAP by its RING domain-dependent E3 ligase activity.89 The RING domain is located at the carboxyl terminus of XIAP and mediates ubiquitylation not only of target proteins, but also XIAP itself, a critical determinant of XIAP stability in vivo.90 Accordingly, experimental mice expressing a mutant of XIAP that lacks the RING domain express abnormally high levels of XIAP protein in their cells.90 Unexpectedly, the enhanced stability of XIAPΔRING did not afford hepatocytes from these mice with enhanced protection from Fas-induced apoptosis.90 Even more surprisingly, the XIAPΔRING mutant protein exhibited abnormally reduced anti-apoptotic activity in murine embryonic fibroblasts exposed to TNFα, indicating that anti-apoptotic activity as well as protein stability of XIAP is determined (in part) by its RING domain.90 Interestingly, the notion that the RING domain of XIAP might be critical for its ability to regulate cell death stemmed from experiments in D. melanogaster.91 The fly IAP homologue DIAP1 is capable of ubiquitylating caspases and thereby exerts an anti-apoptotic effect that is independent of direct blockade of the catalytic site within these proteases. The DIAP1-mediated ubiqutination inactivates the D. melanogaster caspases drICE and DCP1 by a mechanism that is still not fully understood, but might involve proteasomal degradation.92
Studies with gene-targeted (‘knock-out') as well as transgenic mice were employed to determine the function of XIAP. Enforced overexpression of XIAP within the T-lymphocyte lineage under control of the lck promoter revealed that XIAP could inhibit Fas-induced apoptosis in non-transformed cells, although it is noteworthy that the extent of protection was relatively minor, particularly when T lymphocytes were challenged with agonistic antibodies to Fas in vivo.93 Initial analyses showed that XIAP-deficient mice were largely normal, but only a limited number of cell types were studied and mice or cells derived from them were only challenged by a restricted set of stimuli.94, 95 As described above, the mechanisms of Fas-induced apoptosis differ substantially between different cell types, both in vitro and in vivo. Once caspase-8 is activated and processed in response to Fas ligation, it can proteolyse its specific substrates in the cytosol. Its most prominent substrates include the zymogens of effector caspases (-3 and -7;25, 26, 27) and Bid,23, 24 both of which are activated by proteolytic cleavage. Cells sensitive to Fas-induced apoptosis can roughly be classified into two groups (Figure 1): in so-called type 1 cells (e.g. thymocytes), caspase-8-mediated activation of effector caspases (-3 and -7) is efficient and suffices for cell killing without the need for caspase cascade amplification through Bid-mediated engagement of the Bcl-2-regulated apoptotic pathway; on the other hand, in so-called type 2 cells (e.g. hepatocytes), this amplification mechanism is critical for cell killing. It appears likely that the type 1 versus type 2 discrimination will turn out not to be a black or white categorisation, but rather represent the extremes of a spectrum of responses to Fas ligation occupied by different cell types.
Regardless, it has been proposed that the differences between type 1 and type 2 cells relate to the extent of effector caspase activation that can be achieved, and perhaps the abundance of their substrates that need to be proteolysed to achieve cellular demise.59 The extent of effector caspase activation is determined by, amongst other factors, the levels of Fas on the cell surface, the efficiency of DISC formation with recruitment and activation of caspase-8, the efficiency of proteolytic activation of Bid, induction of MOMP with release of apoptogenic factors and, conversely, the levels of inhibitors of these processes, namely c-FLIP and XIAP.
So, what do we know about the molecular factors involved in the decision whether a Fas-sensitive cell behaves more like type 1 or more like type 2? Initially it was thought that type 1-like signalling solely depended on the cell's ability to rapidly form large amounts of DISC, resulting in high concentration of active caspase-8, which could then directly activate effector caspases. Type 2-like signalling, on the other hand, was correlated with a much weaker and slower ability to from the DISC and activate caspase-8, which could only trigger the effector phase of cell death by engaging the tBid-mediated mitochondrial amplification step.96 It was therefore believed that events at the plasma membrane discriminate between type 1 versus type 2 signalling.97 However, the situation seems to be more complex. For one part, tBid is very potent in triggering MOMP; in other words, even at low caspase-8 activity and relatively low levels of Bid proteolysis, the resulting tBid may well be sufficient to trigger full MOMP with consequent efficient activation of downstream events in the Bcl-2-regulated apoptotic pathway. For the other part, increasing caspase-8 activity, for example by downregulating c-FLIP, does not automatically convert type 2 into type 1 cells.98 This can probably be attributed to the action of XIAP,99 which can directly bind and inhibit the effector caspases (-3 and -7; and also active caspase-9, although with much weaker affinity).79 High levels of XIAP would be predicted to attenuate Fas-induced apoptosis signalling at a much more downstream stage, targeting and blocking processed effector caspases. During MOMP, apoptogenic proteins are released from the mitochondrial intermembrane space, including the IAP antagonists Smac/DIABLO100, 101 and Omi/HtrA2,102, 103 which target XIAP among others, resulting in the derepression of active caspases -3, -7 (and possibly also caspase-9). Accordingly, XIAP was identified as a crucial discriminator between type 1 and type 2 Fas-induced apoptosis.59, 104 Remarkably, although loss of Bid renders mice resistant to FasL-induced hepatocyte apoptosis and fatal hepatitis, concomitant loss of XIAP (in bid−/−xiap−/− mice) fully restored sensitivity.59 Loss of XIAP alone (on a bid+/+ background) significantly (albeit to a relatively minor extent) accelerated FasL-induced hepatocyte apoptosis and fatal hepatitis, but had no impact on the rate of FasL-induced apoptosis in thymocytes.59 This indicates that the XIAP-imposed brake on effector caspases is critical for the regulation in Fas-induced apoptosis signalling in type 2, but not type 1 cells, consistent with the notion that the magnitude of the caspase cascade is limiting in the former. In conclusion, whether a cell behaves like type 1 or like type 2 is thus likely to depend not on the levels of a single protein but rather on the ratio between processed effector caspases (-3 and -7), a more or less direct consequence of DISC formation and caspase-8 activation, and the levels of free XIAP. Given the complex post-translational regulation of XIAP stability and activity,90 this ratio is likely to be subject to a large extent of variation as a consequence of a broad range of cell stimuli, even within the same cell type, and would thus be difficult to predict.
Due to their profound liver toxicity (and maybe toxicity in other tissues as well), potent Fas agonists, such as highly aggregated FasL16, 60 or anti-Fas antibodies,105 are unsuited for therapeutic applications, particularly if one aimed to combine Fas-activation with IAP antagonists, which further increase the sensitivity of hepatocytes towards Fas-induced killing.59 It is, however, noteworthy that less potent Fas agonists (some of them developed by Juerg Tschopp), that do not cause unacceptable collateral damage, may find utility in cancer therapy (see Table 1).106 Moreover, the broad concept of type 1/type 2 apoptosis signalling, initially discovered for Fas-induced cell killing, is also pertinent to DR-induced apoptosis activated by TRAIL,107, 108, 109, 110 and (to a certain extent) by TNF69, ligands that are used and/or being developed for cancer therapy.111
Importantly, with respect to the use of activators of the DR apoptotic pathway for cancer therapy, many tumour cells, in particular lymphomas and leukaemias, behave like type 2 cells96 and many of them are relatively resistant to FasL-, TNF- or TRAIL-induced killing, most likely as a consequence of the high levels of Bcl-2, Mcl-1, Bcl-xL and/or XIAP that they express.111 Breaking the resistance towards FasL-, TNF- or TRAIL-induced killing of malignant cells might therefore aid in the development of more efficacious regimes for the treatment of certain cancers. The blockade of effector caspases by XIAP in normal as well as transformed cells is blocked physiologically by IAP antagonists, such as Smac/DIABLO,100, 101 and such de-repression of effector caspases can also be achieved by small molecule Smac/DIABLO mimetics. Accordingly, there is increasing experimental evidence that small molecule IAP antagonists can sensitise resistant cancer cells towards TRAIL and/or TNF-induced killing, either given as single agents, or in combination with classical chemotherapeutic drugs (Figure 3).112, 113, 114, 115, 116 The IAP antagonists available today target multiple members of the IAP family, including cIAP1, cIAP2 and XIAP (see Table 1 for an overview of current clinical trials using IAP antagonists).117 Given the role of cIAP1 and cIAP2 in the regulation of proximal events in TNF-R1 signalling,117, 118 antagonists that are highly specific for XIAP may be even better suited than broad spectrum IAP inhibitors for cancer therapy.
We dedicate this review to the memory of Juerg Tschopp, wonderfully generous human being and outstanding scientist, who left behind not only important scientific discoveries, but also, importantly, the novel treatments for several inflammatory diseases. He will be solely missed. We thank all the present and the past members of the apoptosis research programs at WEHI, particularly Drs JM Adams, S Cory, D Vaux, J Silke, D Huang, P Colman, P Bouillet, R Kluck and C Scott, for their outstanding contributions and stimulating discussions. Research in our laboratories is supported by fellowships and grants from the Australian NHMRC (257502, 461299), NIH (CA 043540), Leukemia and Lymphoma Society (LLS SCOR 7413), JDRF/NHMRC (466658), the Max-Eder Program grant from the Mildred Scheel-Stiftung/ Deutsche Krebshilfe, the Novartis Foundation for Medicine and Biology, and the Swiss National Science Foundation (PP00A—119203).
The authors declare no conflict of interest.
Edited by G Melino