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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Nat Med. Author manuscript; available in PMC 2012 January 3.
Published in final edited form as:
PMCID: PMC3250071
NIHMSID: NIHMS346073

A recombinant Mycobacterium smegmatis induces potent bactericidal immunity against Mycobacterium tuberculosis

Abstract

We report the involvement of an evolutionarily conserved set of mycobacterial genes, the esx-3 region, in evasion of bacterial killing by innate immunity. Whereas high-dose intravenous infections of mice with the rapidly growing mycobacterial species Mycobacterium smegmatis bearing an intact esx-3 locus were rapidly lethal, infection with an M. smegmatis Δesx-3 mutant (here designated as the IKE strain) was controlled and cleared by a MyD88-dependent bactericidal immune response. Introduction of the orthologous Mycobacterium tuberculosis esx-3 genes into the IKE strain resulted in a strain, designated IKEPLUS, that remained susceptible to innate immune killing and was highly attenuated in mice but had a marked ability to stimulate bactericidal immunity against challenge with virulent M. tuberculosis. Analysis of these adaptive immune responses indicated that the highly protective bactericidal immunity elicited by IKEPLUS was dependent on CD4+ memory T cells and involved a distinct shift in the pattern of cytokine responses by CD4+ cells. Our results establish a role for the esx-3 locus in promoting mycobacterial virulence and also identify the IKE strain as a potentially powerful candidate vaccine vector for eliciting protective immunity to M. tuberculosis.

Tuberculosis is a leading cause of global mortality due to infectious disease, and it is a worsening problem owing to HIV co-infection and the emergence of extremely drug-resistant strains of M. tuberculosis (Mtb)1,2. The only currently used vaccine, the attenuated Mycobacterium bovis strain bacille Calmette-Guérin (BCG), has been inconsistent in providing protection from disease, with low or unmeasurable efficacy in many of those regions with the most cases1,3,4. Moreover, BCG can cause severe, disseminated infection in HIV-positive infants, further restricting its use in areas with high prevalence of tuberculosis5. Animal models of tuberculosis also demonstrate that BCG vaccination generates at best partial immunity against Mtb that does not give complete control or clearance of infection6. Thus, new tuberculosis vaccines that are safer and more efficacious than BCG are clearly needed79.

Generation of more effective vaccines requires a better understanding of the mechanisms by which Mtb evades innate and adaptive immune responses6,1014. Some of these mechanisms are known to involve the export of various effector molecules by specialized bacterial secretion systems. For example, the well-studied mycobacterial type VII secretion system known as ESX-1 has been shown to be involved in a range of effects that promote mycobacterial virulence through secretion of the protein ESAT-6 and other effector proteins1525. Notably, the Mtb genome encodes four other potential secretion systems that are paralogs of ESX-1 (designated ESX-2 through ESX-5), but their functions are mostly unknown. Although all mycobacterial species studied to date have between two and five of the esx loci, only the esx-3 genes (comprising the genes Rv0282 to Rv0292 in Mtb and Ms0615 to Ms0626 in M. smegmatis (Msmeg); Supplementary Fig. 1) are conserved in all cases26,27. This suggests a unique and crucial function for ESX-3. Expression of esx-3 genes is regulated by iron and zinc and is increased in the lungs of mice during persistent infection with Mtb2831, suggesting it may have a key role in virulence. In addition, an intact esx-3 locus has been shown to be essential for the growth of Mtb in culture3234.

Because inactivation of esx-3 is incompatible with viability in Mtb, in the current study we have used Msmeg, a mycobacterium that is able to grow without an intact esx-3 locus, to study the influence of this locus on the innate and adaptive immune systems of the host. We observed loss of the ability to evade bactericidal host innate immunity for the Msmeg strain with deletion of esx-3, which we therefore designated by the acronym IKE to signify ‘immune killing evasion’, which is lost in this strain. Although insertion of the esx-3 genes of Mtb into IKE did not reverse the susceptibility of the bacteria to innate immune killing, it did produce a recombinant strain, designated IKEPLUS, that induced a previously unobserved level of bactericidal immunity against Mtb. This new immunization strategy suggests an approach to tuberculosis vaccine development that can potentially induce sterilizing immunity and also reveals a previously undocumented capacity for the mammalian immune system to eliminate virulent mycobacteria from infected tissues.

RESULTS

Role of esx-3 in evasion of innate immunity

A robust T helper type 1 (TH1) cell response has a key role in protective immunity to many intracellular pathogens and may be crucial for effective immunity to Mtb35. To assess mechanisms by which mycobacteria may interfere with development of TH1 cell responses, we analyzed the cytokines induced in vivo in mice after infection with Msmeg mutants with deletions of the esx-1 and esx-3 loci (Supplementary Fig. 2). We constructed these mutants in Msmeg because of previous work indicating that esx-3 cannot be deleted in Mtb, probably because of its essential role in iron acquisition32,34. This is not the case for Msmeg because of the presence of a second siderophore system (exochelin) in that species. As expected, a Δesx-3 Msmeg strain (IKE) was readily isolated and grew normally in iron-replete or iron-deficient medium (Supplementary Fig. 3a). We infected mice intravenously with high doses of either unmodified parental, Δesx-1 or IKE strains of Msmeg and measured serum cytokine concentrations. Whereas parental and Δesx-1 Msmeg induced low or undetectable levels of the interleukin-12 (IL-12) subunit p40 and interferon-γ (IFN-γ), infection with IKE elicited robust secretion of these cytokines. In contrast, IL-6 was strongly induced by parental and Δesx-1 Msmeg but was barely detectable in IKE-infected mice (Fig. 1a).

Figure 1
Role of the esx-3 region in evasion of innate immunity by M. smegmatis in a high-dose infection model. (a) Serum concentrations of IL-12 p40, IL-6 and IFN-γ in mice (C57BL/6) infected intravenously with 5 × 107 CFU per mouse of parental ...

Although Msmeg is a saprophytic organism and generally not considered pathogenic, we found that a high intravenous dose (≥ 1 × 107 colony-forming units (CFU)) of parental or Δesx-1 Msmeg was uniformly fatal in C57BL/6 mice within 7 d (Fig. 1b and data not shown). Administration of the same number of heat-killed or ultraviolet-irradiated bacteria did not result in death (Supplementary Fig. 3b). In clear contrast, all C57BL/6 mice infected with the IKE mutant survived and cleared their infections without apparent sequelae (Fig. 1b). The attenuation of Msmeg IKE was further underscored by its inability to kill recombination-activating gene-1–null mice (Rag1−/−), which lack T and B cells (Fig. 1b), suggesting control of infection by an innate immune response. To explore this possibility, we infected a panel of mouse strains deficient in various innate immune response genes (Supplementary Table 1). Among these, only mice lacking the adaptor protein MyD88 were unable to clear the Msmeg IKE infection and died with kinetics similar to those observed in mice infected with parental Msmeg (Fig. 1b and Supplementary Table 1).

Whereas histological analysis of C57BL/6 mice infected with high-dose (5 × 107 CFU) intravenous parental Msmeg showed substantial pathology in the brain, spleen and liver and particularly in the kidney, mice infected with IKE showed no notable pathology in these tissues (data not shown). To further study the attenuation of IKE, we determined tissue bacterial burdens after high-dose intravenous infection of C57BL/6, Rag1−/− and Myd88−/− mice. For C57BL/6 and Rag1−/− mice, there was rapid clearance of IKE from the lungs and kidneys as early as day 3 after infection, with complete clearance by day 35, whereas the parental and Δesx-1 Msmeg strains showed increasing tissue bacterial burdens during the 3–6 d before death of the mice (Fig. 1c). In contrast, in Myd88−/− mice there was expansion of IKE bacilli after inoculation (Fig. 1d). These findings indicated that a previously unappreciated function of the genes in the esx-3 region is to mediate evasion of a MyD88-dependent innate immune response that normally triggers bactericidal effects and host resistance to Msmeg infection.

We performed a genetic complementation analysis by introducing an integrating cosmid encoding all genes of Msmeg esx-3 (Ms0609–Ms0637) into IKE to generate a strain that showed a partial restoration of virulence, as assessed by tissue bacterial burden (Supplementary Fig. 4a). The failure to completely complement the mutation may have been due to aberrant regulation of the introduced esx-3 genes because of integration into a non-native location on the chromosome. We also infected mice with a strain of Msmeg that had a deletion in only a single esx-3 gene (Ms0615), which showed alterations in cytokine patterns and an attenuation of virulence that were similar to those of IKE (Supplementary Fig. 4b,c). Although we have not determined whether the deletion of Ms0615 has effects on other esx-3 genes, it is noteworthy that complementation of this deletion with a plasmid encoding the entire Msmeg esx-3 region (Ms0600Ms0624) fully reversed the phenotype of the mutant in Rag1−/− mice (Supplementary Fig. 4b–d).

Generation and characterization of IKEPLUS

In contrast to our results with Msmeg and similar to previous studies, we were unable to isolate esx-3 deletion mutants of Mtb32,34. As an alternative approach to study the function of Mtb esx-3, we assessed whether the orthologous Mtb genes could complement the Msmeg Δesx-3 phenotype. Thus, we introduced the orthologous locus of Mtb (Rv0282Rv0292) into the IKE strain by transformation with cosmid pYUB1336 containing Mtb genes Rv0278Rv0303 (Supplementary Table 2), generating IKEPLUS. Despite the high homology of these regions between Mtb and Msmeg (minimum of 44% and up to 85% homology at the protein level for the 11 ORFs), analysis of the immunological properties and virulence of IKEPLUS indicated that the Mtb esx-3 genes were unable to complement the phenotype associated with deletion of the Msmeg esx-3 locus. Thus, measurements of serum cytokine concentrations showed that IKEPLUS induced robust IL-12 p40, p70 and IFN-γ responses similar to those observed with the IKE mutant (Fig. 2a). In spite of the stable integration of the Mtb esx-3 genes into the IKE genome (Supplementary Fig. 5), the IKEPLUS strain was rapidly cleared from tissues (Fig. 2b) and was unable to kill either immunocompetent or Rag1−/− mice but was lethal in MyD88-deficient mice (Fig. 2c).

Figure 2
Characterization of innate immune responses against the Msmeg IKEPLUS strain. (a) Serum concentrations of IL-12 p40, IL-12p70 and IFN-γ in C57BL/6 mice infected with IKE or IKEPLUS (*P < 0.01, ANOVA with Bonferroni post-test). (b) Growth ...

Ability of IKEPLUS to elicit bactericidal immunity against Mtb

The results presented above revealed properties of IKE and IKEPLUS that are desirable for antituberculosis vaccines. These mutants were highly attenuated for virulence in mice, thus fulfilling initial safety requirements, and they rapidly induced a cytokine milieu that was favorable to priming of TH1 responses (Fig. 2a). Furthermore, in the case of IKEPLUS, this recombinant Msmeg contained a cosmid (pYUB1336, Supplementary Table 2; also see Supplementary Table 3) encoding 26 open reading frames of Mtb that had the potential to serve as specific antigens for eliciting adaptive immune responses that could be recalled by subsequent Mtb infection. To examine its potential as an antituberculosis vaccine, we used IKEPLUS to immunize mice (5 × 107 CFU intravenously), which we then challenged 8 weeks later with a high intravenous dose (1 × 107 CFU) of Mtb (virulent strain H37Rv). We immunized control groups either with saline or subcutaneously with BCG (1 × 107 CFU per mouse). The average time to death after Mtb challenge was 54 d for sham-vaccinated mice and 65 d for BCG-immunized mice, whereas the IKEPLUS-immunized mice had a mean survival time of 135 d (Fig. 3a).

Figure 3
Bactericidal immunity against Mtb in mice vaccinated with IKEPLUS. (a) Survival of C57BL/6 mice that were sham immunized (intravenous (i.v.) PBS injection; n = 21) or immunized by intravenous infection with IKEPLUS (5 × 107 CFU per mouse; n = ...

Consistent with the extension of survival observed in a subset of IKEPLUS-immunized mice, we found in three separate experiments that intravenous immunization of mice with IKEPLUS led to marked reductions in tissue bacterial burdens in mice surviving to later time points after Mtb challenge (Fig. 3b). We observed this effect in lung, spleen and liver tissue at several time points after the challenge. Notably, the bacterial burdens of all tissues examined in IKEPLUS-immunized mice showed a continuing decline over time in mice that survived > 100 d. By 200 d after Mtb challenge, these declines exceeded by > 1,000-fold the maximum levels of CFU reduction achieved in those BCG-vaccinated mice that survived to day 100. Furthermore, CFU measurements were below the limit of detection in the livers of IKEPLUS-vaccinated and Mtb-challenged mice surviving >200 d, indicating a high level of bactericidal and potentially sterilizing immunity. In contrast to the long-term survival observed in a subset of IKEPLUS-immunized mice, no BCG-immunized mice survived more than 173 d after challenge, and BCG-immunized mice that survived to 100 d had a greater bacterial burden in all organs than did IKEPLUS-immunized mice (Fig. 3b). The Mtb genes present in IKEPLUS were necessary to induce highly protective immune responses, as mice sham-vaccinated with the parental IKE strain showed survival and bacterial burden after Mtb challenge that were only modestly better than those of naive mice (Fig. 3c).

Histopathological evaluation also revealed evidence of robust immunity in IKEPLUS-vaccinated mice. At 100 d after Mtb challenge, all mice showed moderate-to-severe diffuse granulomatous pneumonia, and at this time point there were no apparent differences in the lung histology (Fig. 4a and Supplementary Fig. 6) or severity of pneumonia on the basis of quantitative visual scoring (Fig. 4b) between mice immunized with saline, BCG or IKEPLUS. The severity of granulomatous inflammation in the spleens and livers from all treatment groups were likewise similar at day 100 after Mtb challenge (Fig. 4b and data not shown). However, lung tissues from IKEPLUS-immunized mice surviving >200 d after Mtb challenge showed clear evidence of gradually resolving granulomatous inflammation as compared to lung tissue at day 100, along with improvements in microscopic appearance (Fig. 4a) and significant reductions in the histological scores (Fig. 4b) in both lung and liver sections. Notably, we continued to observe low numbers of acid-fast bacilli (AFB) in small granuloma-like structures in the livers of IKEPLUS-immunized mice killed at more than 200 d after Mtb challenge (Fig. 4a), despite the lack of culturable bacilli in these tissues.

Figure 4
Improvement of histopathology in IKEPLUS-immunized mice during resolution of Mtb infection. (a) Representative images are shown for lungs (left) and livers (right) of C57BL/6 mice immunized with IKEPLUS and challenged with Mtb as described in Figure 3 ...

Efficacy of subcutaneously administered IKEPLUS as a vaccine

The unusually potent immune response elicited by intravenously administered IKEPLUS led us to assess its potential as a subcutaneously administered vaccine against tuberculosis in mice. As a first approach, we immunized mice subcutaneously with BCG or IKEPLUS and 8 weeks later challenged them with a high intravenous dose (1 × 108 CFU) of Mtb. Compared to BCG-immunized mice, mice immunized subcutaneously with IKEPLUS had significantly (P < 0.01) more serum IL-12 p40 upon challenge with Mtb (Supplementary Fig. 7a). Furthermore, mice immunized subcutaneously with IKEPLUS survived significantly longer after Mtb challenge than did BCG-immunized mice (Fig. 5a). Quantification of tissue bacterial burdens showed that subcutaneous immunization of mice with IKEPLUS led to an ~3-log reduction of CFU in the lung at 20 d after Mtb challenge compared to lung tissue in saline-immunized mice, which was nearly 2 logs better than the level of protection from BCG immunization (Fig. 5a).

Figure 5
Protection from Mtb challenge by subcutaneous immunization with IKEPLUS. (a) Survival (left) of C57BL/6 mice (n = 10–14 per group) immunized subcutaneously with PBS, IKEPLUS (1 × 108 CFU per mouse), or BCG (1 × 106 CFU per mouse) ...

To assess the vaccine efficacy of IKEPLUS in a more physiological setting, we immunized mice subcutaneously and then challenged them 1 month later by low-dose aerosol infection with Mtb (~100 CFU per mouse). Mice immunized subcutaneously with IKEPLUS showed a trend toward longer survival after Mtb challenge compared to BCG-immunized mice (Fig. 5b; mean survival 301 d versus 267 d; P = 0.0898, log-rank test). IKEPLUS vaccination resulted in significant reductions in CFU in lung and spleen tissues that were comparable to those achieved with BCG at 4 weeks (Fig. 5b). However, at 25 weeks after Mtb challenge, the reduction in CFU for IKEPLUS-immunized mice was maintained, whereas bacterial burdens in BCG-immunized mice increased to the level observed in sham-vaccinated mice (Fig. 5b).

Central role for CD4+ T cells in immunity induced by IKEPLUS

The ability of IKEPLUS to enhance IL-12 p40 and IFN-γ production suggested enhanced TH1 cell responses as a mechanism for heightened protective immunity in IKEPLUS-immunized mice. This idea was supported by cytokine profiles elicited in the immunization-challenge model (Supplementary Fig. 7), as mice immunized with either IKE or IKEPLUS showed rapid induction of IL-12 p40 and IFN-γ expression in the first 2 d after high-dose intravenous challenge with Mtb (Supplementary Fig. 7b). These data suggested that the protective responses elicited by IKEPLUS immunization were associated with the induction of TH1-type adaptive immunity. This hypothesis was further supported by our findings that Rag1−/− and IL-12 p40–deficient mice showed no significant change (P > 0.25) in their high susceptibility to Mtb challenge after immunization with IKEPLUS (Supplementary Fig. 8a).

As adaptive T cell immunity requires priming through peptide antigen presentation by major histocompatibility complex (MHC) class I and MHC class II proteins, we analyzed the requirement for these molecules in the induction of protective immunity by IKEPLUS. We immunized MHC class I–deficient and MHC class II–deficient mice with IKEPLUS (intravenously), BCG (subcutaneously) or saline and then challenged them with a high intravenous dose (1 × 107 CFU per mouse) of virulent Mtb. Determination of bacterial loads showed that MHC class I–deficient mice immunized with IKEPLUS had significantly higher (P < 0.05) protection compared to saline-immunized MHC class I–deficient mice upon challenge with Mtb at both the 2-week and 4-week time points (Supplementary Fig. 8b). In contrast, the bacterial loads in IKEPLUS-immunized MHC class II–deficient mice killed at both 2 weeks and 4 weeks after challenge were not significantly different (P > 0.05) from those of saline-immunized MHC class II–deficient mice (Supplementary Fig. 8b). This suggests that the protective immunity elicited by IKEPLUS immunization is dependent on MHC class II presentation and therefore probably involves responses by CD4+ T cells.

To further examine the involvement of CD4+ T cells, we assessed the ability of purified T cell populations from IKEPLUS-immunized mice to transfer protection against Mtb challenge to naive mice. We immunized mice intravenously with IKEPLUS or saline or subcutaneously with BCG. After 3–6 weeks, we purified CD4+ T cells from the spleens of immunized mice and then transferred them to naive mice. These mice were then challenged with a high intravenous dose (1 × 107 CFU per mouse) of Mtb. Lung CFU counts 3 weeks after challenge showed that significant protection was transferred by CD4+ T cells from IKEPLUS-vaccinated donors, with a reduction in CFU nearly equivalent to that in mice that had been directly immunized with either intravenous IKEPLUS or subcutaneous BCG (Fig. 6a). Survival was also significantly extended in mice receiving CD4+ T cells from IKEPLUS-immunized donors (Fig. 6a). As expected, CD4+ T cells from sham-vaccinated mice did not transfer protection in this experiment, nor did CD4+ T cells from BCG-vaccinated donors, consistent with earlier studies showing that transfer of BCG-induced immunity requires sublethal irradiation of recipients36. There was no transfer of protection using purified CD8+ T cells from any of the donor groups. Thus, the CD4+ T cell population from IKEPLUS-immunized mice seemed to contain the main pool of memory T cells responsible for activating protective immunity to challenge with Mtb.

Figure 6
Role of CD4+ T cells in IKEPLUS-induced protective immunity. (a) Contributions of CD4+ and CD8+ subsets, as assessed by adoptive transfer of T cells from IKEPLUS-immunized mice. Naive recipient mice (n = 5 per group) were challenged 1 d after T cell transfer ...

We examined the multifunctionality of the CD4+ T cell response in IKEPLUS-immunized mice by analyzing cytokine production by CD4+ T cells infiltrating the lung at various time points after Mtb challenge (Fig. 6b–d and Supplementary Fig. 9). Upon re-stimulation in vitro with a peptide corresponding to an MHC class II (I-Ab)-presented epitope of the TB9.8 (esxG) protein (Supplementary Fig. 1), we observed a substantially different pattern of cytokine production between BCG- and IKEPLUS-immunized mice (Fig. 6b). Using simultaneous analysis of three cytokines (IFN-γ, tumor necrosis factor (TNF) and IL-2), we observed an increase of CD4+ T cells producing two or three of these cytokines in IKEPLUS-immunized compared with BCG-immunized mice (Fig. 6b,d) in response to stimulation by TB9.8. This was particularly evident at 2 or more weeks after Mtb challenge. In marked contrast, CD4+ T cells producing two or three of these cytokines accumulated more gradually in the IKEPLUS-immunized mice, but their levels were sustained to the latest time points examined (that is, day 36). We observed a similar pattern in total lung-derived CD4+ T cells after re-stimulation in vitro with CD3-specific antibody (Fig. 6c,d).

DISCUSSION

In the current study we have demonstrated a major role for the esx-3 locus of Msmeg in modifying the mammalian host immune response, and in so doing we have generated a new and highly effective candidate vaccine for tuberculosis. Although Msmeg is a saprophytic organism rarely reported as a cause of clinical infections37,38, our high-dose intravenous inoculation protocol in mice revealed its ability to establish a rapidly fatal experimental infection. This cryptic virulence of Msmeg was dependent on an intact ESX-3 secretion system, as deletion of the esx-3 genes generated a strain (IKE) that was rapidly cleared without sequellae. We observed clearance of IKE in mice lacking various components of innate and adaptive immunity, but not in MyD88-deficient mice. Overall, these findings are consistent with a role for ESX-3 in mediating resistance to MyD88-dependent bactericidal effects in mice. As mice defective in Toll-like receptor 2 and IL-1R signaling retained resistance to high-dose infection with IKE (Supplementary Table 1), these MyD88-dependent receptors are probably not involved.

How the esx-3 locus contributes to resistance to MyD88-dependent killing of mycobacteria remains to be determined. It is possible that this effect is related to the iron-acquisition function of ESX-3, although many other possibilities can be envisioned. For example, ESX-3–secreted substrates may directly interact with innate immune signaling or effector molecules and modulate the response to intracellular infection. So far, only one secreted substrate, EsxH (TB10.4) has been directly documented for this system34. However, the Cfp-10 paralog EsxG (TB9.8) is also likely to be secreted by ESX-3, and annotation of the esx-3 locus reveals genes for proteins belonging to the PE and PPE families, other members of which are known to be exported by the ESX-5 system and to modulate macrophage responses39. As in the case of ESX-1, the secreted substrates of ESX-3 may potentially be encoded by genes mapping to chromosomal sites outside the locus encoding the secretion system40.

Inoculation with IKE induced a more robust TH1-priming cytokine milieu and a more rapid and vigorous IFN-γ response as compared to inoculation with the parental Msmeg strain. Preliminary analysis also suggested that IKE stimulated increased phagosome maturation during macrophage infection (K.A.S. and W.R.J. Jr., unpublished data), which could lead to increased processing and presentation of bacterial antigens. When we added Mtb antigens from a cosmid encoding genes Rv0278Rv0303 to IKE, we derived a strain (IKEPLUS) that elicited a high level of protective immunity when used as a live attenuated vaccine against Mtb in mice. These findings contrast with many earlier studies examining Msmeg as a vaccine vector delivered either intranasally or subcutaneously, which showed no significant improvement in efficacy compared to BCG4143. We hypothesize that the superior vaccine potency of IKEPLUS is attributable to the loss of innate immune evasion mediated by esx-3. The modified innate immune response to Msmeg lacking esx-3 generates a cytokine milieu that favors the priming of TH1 cell responses, as indicated by the enhanced systemic IL-12 and IFN-γ levels observed. This, in turn, leads to an improved adaptive immunity, which may be directed against the Mtb antigens encoded by IKEPLUS.

Notably, the robust adaptive immune response induced by IKEPLUS was potent and long-lived, as indicated by the marked decline in Mtb bacilli in the tissues of IKEPLUS-immunized animals. To our knowledge, neither BCG nor any of the attenuated Mtb vaccine strains described to date induces such obvious or prolonged bactericidal immunity. Several reports have described modified-BCG or other novel tuberculosis vaccine regimens that deliver modestly better protective immunity against Mtb than does standard BCG (for example, up to a 1-log improvement in reduction in CFU counts in the lungs)6,4449. However, none of these ‘improved’ tuberculosis vaccines has been shown to produce the sustained decline or magnitude of reduction (>3 logs) in Mtb tissue burdens that we observed in IKEPLUS-immunized mice.

Most notably, our observation of apparent sterility in the livers of IKEPLUS-immunized animals surviving past 200 d after Mtb challenge may represent the first documented example of the elimination of this organism from a tissue by immunological mechanisms alone. These findings reveal a capacity of the mammalian adaptive immune system to orchestrate potent bactericidal sterilization in Mtb-infected tissues. This result was most apparent when we administered IKEPLUS intravenously, which is not a feasible route for standard vaccination, and even with intravenous inoculation only a small fraction (10–20%) of IKEPLUS-immunized mice achieved long-term survival after Mtb challenge. Thus, further improvements will be needed to optimize the efficacy of IKEPLUS vaccination for translational development and implementation as a vaccine in humans. This may involve combining IKEPLUS immunization with other types of immunogens in prime-boost regimens. Nevertheless, the substantial bactericidal immunity we observed after subcutaneous immunization with IKEPLUS in both the intravenous and aerosol Mtb challenge models is encouraging. Although the magnitude of this immunity was not as great as that observed after intravenous immunization, it was still substantially greater and longer lasting than the protection induced by standard BCG vaccination. We are currently in the process of determining which genes of the integrated cosmid in IKEPLUS are required for induction of protective immunity and are carrying out further modifications with the intent of increasing the vaccine potency of IKEPLUS when administered by clinically acceptable routes.

Determining of the mechanism of bacterial killing in IKEPLUS-immunized mice is clearly a crucial next step. Our finding that transfer of CD4+ T cells from IKEPLUS-immunized mice into naive mice was sufficient to confer substantial protection strongly suggested that this mechanism involved generation of a unique memory CD4+ T cell population. To the best of our knowledge, this is the first example in an Mtb infection model of adoptively transferred immunity from immunized mice to naive mice without previous irradiation for lymphocyte depletion of the recipient mice36. This further suggests that the response stimulated by IKEPLUS is qualitatively different from that induced by BCG, or unusually potent. A qualitative effect on the CD4+ T cell response was also indicated by increased multifunctionality of the cytokine-producing cells responding to the TB9.8 antigen in the lungs of IKEPLUS-immunized mice after Mtb challenge. Notably, we also observed this pattern for CD4+ T cells from IKEPLUS-vaccinated mice stimulated ex vivo by monoclonal anti-CD3 antibody, suggesting that this multifunctional program had been imprinted on the great majority of CD4+ T cells accumulating after Mtb challenge in the lungs of IKEPLUS-immunized but not BCG-immunized mice.

Although CD4+ T cells seem to be the crucial memory cells involved in initiating the bactericidal response, it is likely that these cells recruit additional effectors that may ultimately be partially or entirely responsible for the actual killing of the mycobacteria. Such downstream effectors could include various myeloid subsets and innate-like lymphocytes with potent effector functions such as natural killer cells, NKT cells or γδ T cells50. B lymphocytes and secreted antibodies may also have a role, as preliminary experiments using western blotting to assess antimycobacterial antibody responses in the sera of immunized and challenged mice showed marked differences between IKEPLUS- and BCG-immunized mice (K.A.S. and W.R.J. Jr., unpublished data). Determining the interplay between these various immune effector mechanisms in bringing about clearance of newly introduced or established Mtb infections will be a major focus of further studies on the use of IKEPLUS as a candidate tuberculosis vaccine.

Methods

Methods and any associated references are available in the online version of the paper at http://www.nature.com/naturemedicine/.

Supplementary Material

Supplementals

01

Acknowledgments

We acknowledge the support of US National Institutes of Health (NIH) grants AI063537, AI093649, AI092448, AI26170 and AI051519 (Einstein Center for AIDS Research), and the Bill and Melinda Gates Foundation Collaboration for AIDS Vaccine Discovery. K.A.S. acknowledges support from the Albert Einstein College of Medicine’s Institutional AIDS Training Grant T32-AI007501. We thank C. Harding, L. Ramachandra, G. Lauvau and S. Morris for advice and suggestions, as well as C. Colon-Berezin and S. Tiwari for helpful editing of the manuscript. Flow cytometry studies were carried out using core facilities supported by the Einstein Cancer Center (NIH/National Cancer Institute CA013330) and the Einstein Center for AIDS Research (NIH AI051519).

Footnotes

Note: Supplementary information is available on the Nature Medicine website.

AUTHOR CONTRIBUTIONS

K.A.S. constructed bacterial strains, performed or contributed to the design of most experiments, and analyzed and interpreted data. D.N.D. carried out portions of the infection and challenge experiments. M.F.G. contributed to the T cell adoptive transfer studies and designed, performed and analyzed all flow cytometry analyses. T.H. participated in design and construction of bacterial strains and in the performance of mouse infection and challenge experiments. P.J. contributed to construction of bacterial strains. M.M.V. and M.H.-T. assisted with experiments analyzing responding T cell populations. R.S.S. analyzed and scored the histopathology samples. B.C., M.C., J.K. and R.L. carried out mouse infections, organ harvesting and quantification of bacilli in tissues. D.O., J.C., I.M.O., S.A.P. and W.R.J. Jr. designed and interpreted experiments. K.A.S., S.A.P. and W.R.J. Jr. wrote the manuscript.

COMPETING FINANCIAL INTERESTS

The authors declare no competing financial interests.

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