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Cytolocation of R6-EGFP. (A) 293 cells were transfected with 5 μg of plasmids encoding EGFP or R6-EGFP with the aid of GeneJuice, at 48 h post-transfection a Western blotting analysis was performed on cell extracts (30 μg protein) and membranes were hybridized with an antibody against GFP. (B) (a to f) C2C12 myoblasts were cotransfected with pR6-EGFP and pRFP1-N1-GalT, at 48 h post-transfection, cells were incubated with 25 mM glucose (a to c) or without glucose (d to f) for 16 h and a colocation analysis of EGFP and RFP was performed; (g to l) C2C12-mtRFP myoblasts were transfected with pR6-EGFP, at 48 h post-transfection, cells were incubated with 25 mM glucose (g to i) or without glucose (j to l) for 16 h and then colocation analysis of EGFP and RFP was performed. The image shows the fluorescent signal of EGFP (a, d, g, j) and RFP (b, e, h, k) and the colocation of the signal of EGFP and RFP (c, f, i, l).