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Glycogen particles. Cultured human myotubes were transduced with Ad-GFP, Ad-R6, Ad-PTG or Ad-GM and incubated with 25 mM glucose for 48 h and then fixed and stained for observation of glycogen particles in transmission electronic microscopy. Representative images are shown: (A) image of myotubes obtained at × 40000 magnification, in which glycogen granules appear as dense spheroid particles, and (B) a detail of glycogen granules (arrows). Scale bars = 100 nm.