Glycogen synthesis and content. Cultured human myotubes were: (A) transduced with Ad-GFP (▲) or Ad-R6 (■) and incubated with glucose-deprived medium for 18 h, then with 10 mM [U-14C]glucose for the times indicated and harvested to quantify the radioactivity associated with glycogen; (B) transduced with Ad-GFP, Ad-R6, Ad-PTG or Ad-GM, incubated with 25 mM glucose for 48 h and harvested to assess glycogen content. (A, B) Data are means ± SEM from three experiments performed in duplicate. Significance of differences versus cells treated with Ad-GFP: *p < 0.05 and **p < 0.001. (C) C2C12 cells were transduced with Ad-lacZ, Ad-R6, Ad-PTG or Ad-GM and incubated with 25 mM glucose for 72 h. Fixed cell monolayers were treated without (-) or with (+) alpha-amylase and glycogen stained with fluorescent PA-SH. A representative image is shown.
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