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Effect of PPP1R6 overexpression on GS and GP activity. Cultured human myotubes were transduced with Ad-GFP, Ad-R6 or/and Ad-MGP and incubated with 25 mM glucose for 48 h. (A) Immunoblot analyses were performed on extracts (20 μg protein) from cells transduced with Ad-GFP or Ad-R6. Membranes were hybridized with antibody against PPP1R6. Enzyme activities were assessed in cell extracts: (B) GS activity measured with (black columns, total activity) or without (white columns, activity of the active form) glucose 6-phosphate and (C) GP activity measured with (black columns, total activity) or without (white columns, activity of the active form) AMP. Data are means ± SEM from two experiments performed in triplicate. Significance of differences versus cells treated with Ad-GFP under the same incubation conditions: *p < 0.001 and **p < 0.0001.