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Despite the fact that approximately half of all HIV patients acquire infection through penile exposure, there have been no recent studies of penile SIV transmission in rhesus macaques and the nature of the virus variants transmitted, target cells, and pathways of virus dissemination to systemic lymphoid tissues are not known. Single genome amplification (SGA) and sequencing of HIV-1 RNA in plasma of acutely infected humans allows the identification and enumeration of transmitted/founder viruses responsible for productive systemic infection. Studies using the SGA strategy have shown that intrarectal and intravaginal SIV transmission to macaques recapitulates key features of human HIV transmission. To date, no studies have used the SGA assay to identify transmitted/founder virus(es) in macaques infected after penile SIV exposure. Here we report that SIV can be transmitted by penile SIV exposure. However, similar exposure to a high-dose inoculum infects only about half the animals, which is about 50% less efficient transmission than occurs after vaginal SIV challenge. In addition, only a single SIV env variant established the systemic infection in all five animals that became infected after penile exposure, a result that is consistent with low incidence and few transmitted HIV variants in heterosexually infected men. Our results suggest that the penile transmission of SIVmac251 in rhesus macaques recapitulates the key features of penile HIV-1 transmission and may provide insight into host or viral factors that permit penile transmission and dissemination. Furthermore, this SIV challenge exposure route will be useful in testing vaccines and other prophylactic approaches.
HIV is primarily transmitted by heterosexual contact and approximately equal numbers of men and women are infected with the virus worldwide. Numerous studies to define the biology of HIV transmission have been undertaken with the goal of identifying stages of the infection process, from mucosal exposure through dissemination to distal lymphoid tissues establishment of systemic infection, to identify targets for interventions. To a large extent this effort has focused on understanding rectal transmission in men and vaginal transmission in women,1–22 but heterosexual men are regularly infected through penile HIV exposure. Despite the fact that approximately half of HIV patients acquire infection through penile exposure,23–30 there have been no preclinical studies of AIDS vaccines using penile inoculation to challenge immunized animals and nothing is known about the anti-HIV immune effector mechanisms that may be present on the mucosal surfaces of the penis, which presumably serves as the portal of entry for HIV transmission to males. The foreskin is thought to be particularly important in HIV transmission to males because the presence of an intact foreskin is associated with an approximately 50% increased risk of HIV acquisition.30–36 The foreskin and glans of the human penis have a complete population of immune cells including potential HIV target cells.37–39 Although the foreskin is clearly a critical target tissue for HIV transmission, and circumcision can reduce HIV acquisition,31–33 other tissues of the penis must account for the remaining transmissions in 50% of HIV-infected men.
Strategies to prevent penile HIV transmission should be directed at the viral variants responsible for establishing productive infection in men via this route of exposure. Identifying the individual HIV-1 variants that are transmitted and establish systemic infection is possible using single genome amplification (SGA) and direct sequencing, a technique that provides an accurate characterization of the transmitted/founder viruses involved in the initial establishment of systemic infection following mucosal transmission.40,41 Data generated using the SGA approach and recent empirical data together with mathematical models have shown that immediately after transmission, in the absence of selective pressure, a founder HIV-1 lineage typically diversifies by a Poisson distribution of random nucleotide substitutions leading to a star-like phylogeny of viral variants in the HIV quasispecies with no or few shared mutations.42,43 For each low-diversity HIV-1 lineage in an individual, the consensus of the sequences within that lineage represents the inferred ancestral sequence. If sampled prior to the adaptive immune response or the onset of other selection pressures, mutations occur randomly and thus the consensus sequence of each low diversity lineage represents a transmitted or founder virus sequence.43
Using the SGA sequencing approach and mathematical modeling, it has now been reported in at least eight patient cohorts representing HIV-1 subtypes A, B, C, and D that most (60–90%) mucosal infections originate from single variant transmissions.41,43–47 The remaining 10–40% of infections are initiated by a limited number of transmitted/founder HIV-1 variants. Therefore, for each individual infected, the potential viral diversity in the period of acute infection is limited to a single or few HIV-1 lineages. This genetic bottleneck is less pronounced in individuals engaged in behaviors that may plausibly facilitate transmission (anal-receptive intercourse or intravenous drug use)43,48,49 and in patients with sexually transmitted infections.41,44–47 Importantly, acute infection with ”heterogeneous” HIV populations has been linked to more rapid disease progression.50–52 The study of the number of transmitted viral variants and their overall diversity can thus have important implications for developing both prophylactic vaccines and antiviral therapy.
Simian immunodeficiency virus (SIV) models of mucosal HIV transmission in rhesus macaques are valuable for examining the transmitted or early founder populations that establish productive infection because the genetic composition of the virus stock used for mucosal inoculation can be readily defined and compared to the viruses transmitted following mucosal challenge. Furthermore, because the timing of SIV transmission is known,4,6,9,53–55 mathematical modeling is not needed to infer the timing of virus transmission in macaques. SGA approaches can be used to define mucosal challenge conditions for NHP models that recapitulate typical HIV-1 transmissions in humans, resulting in transmission of one, or only a few viral variants, providing animal models for evaluation of vaccines and other prophylactic interventions that recapitulate the virology of HIV transmission. Using the SGA sequencing approach, one to five transmitted/founder viruses were found in rhesus macaques infected intrarectally (i.r.) either SIVmac251 or SIVsmE660.17 In a separate titration study, a dilution of challenge stock was found that reproducibly infected animals with one or only a few viruses following intrarectal challenge.56 Finally, rhesus macaques vaginally challenged with SIVmac251 became systemically infected with 1–10 founder viruses.11 To date, no studies have used the SGA sequencing approach to enumerate and identify transmitted/founder viruses after penile SIV transmission.
Here we report that SIV can be transmitted to rhesus macaques by penile SIV exposure. However, two exposures to a high-dose inoculum on the same day infected only about half the inoculated animals. In addition, only a single SIV env variant established the systemic infection in all five animals that became infected after penile inoculation, a result that is consistent with low incidence and few transmitted HIV variants in heterosexually infected men. Our results suggest that the penile transmission of SIVmac251 in rhesus macaques recapitulates the key features of penile HIV-1 transmission57,58 and may provide insight into host or viral factors that permit penile transmission and dissemination. Furthermore, this inoculation strategy could be useful in testing vaccines and other prophylactic approaches.
The mature male rhesus macaques (Macaca mulatta) used in these studies were housed at the California National Primate Research Center in accordance with the regulations of the American Association for Accreditation of Laboratory Animal Care. These experiments were approved by the Institutional Animal Use and Care Committee of the University of California Davis. All animals were negative for antibodies to HIV-2, SIV, type-D retrovirus, and simian T cell lymphotropic virus type 1 at the time the study was initiated. When necessary, animals were anesthetized with ketamine hydrochloride (10mg/kg; Parke-Davis, Morris Plains, NJ) or 0.7mg/kg tiletamine HCl and zolazepan (Telazol, Fort Dodge Animal Health, Fort Dodge, IA) injected intramuscularly.
A cell-free stock of SIVmac251 (UCD-6/04) was produced in Staphylococcus enterotoxin A-stimulated rhesus monkey peripheral blood mononuclear cells (PBMCs) and used for these studies. This SIVmac251 stock (UCD-6/04) contains approximately 109 viral RNA (vRNA) copies/ml and 105 50% tissue culture infection dose (TCID50)/ml when titered on CEMX174 cells. On the day of challenge, serial 10-fold dilutions of the SIV stock were made in phosphate-buffered saline (PBS) (Table 1); 2ml of virus inoculum was used for all the penile exposures in this study. Animals were exposed repeatedly, at weekly intervals with the more dilute inoculum, and to two exposures to the undiluted SIVmac251 stock on the same day. Animals that did not become infected after the first inoculation with undiluted virus were inoculated using the same procedure to more times at 4 weeks intervals. For the challenge, a small cup was fashioned from a closed end of a disposable 15-ml conical centrifuge tube and 2ml of virus inoculum was placed into the cup. The penis was extended, the foreskin was retracted, and the glans and shaft were inserted into the inoculum and held there for 5min. Then the penis was allowed to retract into the foreskin and the animal was placed into its cage in dorsal recumbency and allowed to recover from anesthesia. Thus although no effort was made to specifically inoculate the foreskin, we visually confirmed that some of the inoculum did contact the area when the glans retracted into the foreskin. Five of the 11 animals in this study were repeatedly exposed with several doses of the virus, but the inoculation series was stopped when vRNA was detected in plasma collected 10–14 days after each challenge.
Plasma and virus stock were thawed at room temperature and RNA was isolated from 0.5ml plasma using the QIAamp Ultrasens Viral Kit (QIAGEN Inc., Valencia, CA) according to the manufacturer's protocol and eluted in 50μl. The vRNA was reverse transcribed into cDNA using SuperscriptIII reagents (Invitrogen, Carlsbad, CA) with 2μl 50μM dT23VN (or gene-specific primer for SGA analysis: SIVEnvR1 5′-TGTAATAAATCCCTTCCAGTCCCCCC-3′), 2μl 10mM dNTPs, and 22μl viral RNA. This mixture was heated to 65°C for 5min followed by incubation on ice for 2min. A master mix of the following was then added: 8μl of 5×First-Strand Buffer, 2μl of 0.1M DTT, 2μl of RNaseOUT Recombinant RNase Inhibitor (40 units/μl), and 2μl of SuperScriptIII RT (200 units/μl) and Incubated at 25°C for 5min, 50°C for 60min, and 70°C for 15min followed by adding 1μl Escherichia coli RNase H (5U/μl) and incubated at 37°C for 20min. cDNA was stored at −20°C to 80°C until amplification.
Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect and quantify SIVgag, RNA levels in plasma samples. Samples were tested in replicates of four reactions carried out in 96-well optical plates (Applied Biosystems, Foster City, CA) in a 25μl reaction volume containing 5μl cDNA and 20μl Mastermix (Applied Biosystems) using the ABI 7900 robotic thermocycler. All sequences were amplified for 2min at 50°C and 10min at 95°C followed by 50 cycles of 15s at 95°C and 1min at 60°C. The following primer pairs and probes were used: SIVgag forward primer 1, 5′-3′ GGG AGA TGG GCG TGA GAA A, reverse primer, CGT TGG GTC GTA GCC TAA TTT T, and probe TCA TCT GCT TTC TTC CCT GAC AAG ACG GA. The copy number of SIVgag was calculated based on standard curves for a viral gene plasmid spanning a concentration range from 0.1 to 108 copies. Although amplification from wells with 0.1 copy of SIVgag was inconsistent, the assays consistently detected SIVgag in wells containing one or more copies of the plasmid. The results were analyzed with SDS 7900 system software version 2.3 (Applied Biosystems). The results for each sample are reported as log10 vRNA copies per ml of plasma RNA.
In a series of preliminary studies conducted separately to evaluate the specificity and determine the background of the PCR assay, RNA isolated from plasma from six rhesus macaques that had never been exposed to SIV was subjected to amplification. There was no amplification of SIVgag from any of these samples. Thus, 50 SIVgag RNA copies/ml plasma (or 1.7 log10 SIVgag copies/ml plasma) was used as the cutoff for determining if a sample from an SIV-inoculated monkey was positive.
The entire env gene was sequenced using a limiting dilution PCR so only one amplifiable molecule is present in each reaction. cDNA was serially diluted and distributed among independent PCR reactions to identify a dilution where amplification occurred in <30% of the total number of reactions. PCR amplification was performed in the presence of 1× buffer, 2mM MgSO4, 0.2mM of each deoxynucleoside triphosphate, 0.2μM of each primer, and 0.025U/μl Platinum Taq High Fidelity polymerase (Invitrogen) in a 20-μl reaction. First round PCR was performed with sense primer SIVEnvF1 5′-CCT CCC CCT CCA GGA CTA GC-3′ and antisense primer SIVEnvR1 5′-TGT AAT AAA TCC CTT CCA GTC CCC CC-3′ under the following conditions: 1 cycle of 94°C for 2min, 35 cycles at 94°C for 15s, 55°C for 30s, and 68°C for 4min, followed by a final extension of 68°C for 10min. Next, 1μl from first-round PCR product was added to a second-round PCR reaction that included the sense primer SIVEnvF2 5′-TAT AAT AGA CAT GGA GAC ACC CTT GAG GGA GC-3′ and antisense primer SIVEnvR2 5′-ATG AGA CAT RTC TAT TGC CAA TTT GTA-3′ performed under the same conditions used for first-round PCR, but with a total of 45 cycles. Correct sized amplicons were identified by agarose gel electrophoresis and directly sequenced with second-round PCR primers and six SIV-specific primers using BigDye Terminator technology. To confirm PCR amplification from a single template, chromatograms were manually examined for multiple peaks, indicative of the presence of amplicons resulting from PCR-generated recombination events, Taq polymerase errors, or multiple variant templates. Confirmation that sequences were derived from individual template molecules ensures proportional representation of individual env sequences circulating in vivo. Sequences containing two or more ambiguous sites were excluded from analysis.
Sequences were aligned using Clustal W60 and hand edited using Jalview61 or MacClade 4.08 to improve alignment quality. All trees were constructed with Phylip62 using the neighbor-joining method63 with the Kimura two-parameter distance matrix.64 Within-subject env diversity was analyzed in three ways, as described in detail previously,43 but fell only into “homogeneous” diversity. Briefly, diversity was determined by visually inspecting sequences by using neighbor-joining phylogenies and the Highlighter tool (www.hiv.lanl.gov). Also, since the population grows exponentially during the early phase of infection, distribution of pairwise Hamming distances (HD) within each sample were examined for star-like phylogeny and a single Poisson distribution of changes indicating infection from a single viral variant.65 Lastly, mathematical modeling was used to test predictions of expected maximum HD against assumptions of infection variant phylogenies.65 All sequences were deposited in GenBank with accession numbers JF298213–JF298241.
Since no infectivity data were available for penile SIV transmission in rhesus macaques, this study was performed by sequentially escalating the concentration of the virus inoculum from 10 to 105 TCID50. None of five animals inoculated 14 times with 10 TCID50 of the SIVmac251 stock became plasma vRNA+ after penile inoculation (Table 1, Fig. 1A). Neither of the two animals inoculated seven times with 103 TCID50 of the SIVmac251 stock became systemically infected after penile inoculation (Table 1, Fig. 1B) and none of the two animals inoculated 14 times with 103 TCID50 of the SIVmac251 stock became systemically infected after penile inoculation (Table 1, Fig. 1B). One animal inoculated with 104 TCID50 of the SIVmac251 stock became systemically infected after one penile inoculation (Table 1, Fig. 1C), but neither of the two animals inoculated seven times with 104 TCID50 became systemically infected (Table 1, Fig. 1C). Three of the five animals inoculated twice in a single day (4h interval between inoculations) with the undiluted SIVmac251 stock (105 TCID50) became systemically infected (Table 1, Fig. 1D). One of the two animals inoculated twice in a single day (4h interval between inoculations) with 105 TCID50 of the SIVmac251 stock every 4 weeks for 8 weeks became systemically infected after penile inoculation with plasma vRNA levels consistent with other infected animals (Table 1, Fig. 1D). Once infected, the pattern of plasma viremia in animals infected by penile SIV inoculation was similar to plasma vRNA in female rhesus macaques infected after vaginal SIV inoculation.10 SIV RNA was first detected in plasma collected at 7–10 days postinfection (PI), however, vRNA peaked at 14–21 days PI (Table 1, Fig. 1), which is delayed compared to vaginal inoculation.10 In both males (Fig. 1) and females,10 a variable set point plasma vRNA level is established at 6–8 weeks PI after genital inoculation.
Phylogenetic relationships among the env sequences in the SIVmac251 (UCD-2/02) stock that was produced from the same seed stock that was used produce the SIVmac251 (UCD-6/04) stock for this study have been described.11 To characterize the SIVmac251 (UCD-6/04) stock used in the present study, a total of 36 env nucleotide sequences were derived by SGA. Sequence diversity was analyzed by pairwise sequence comparison, neighbor-joining phylogenetic tree reconstruction (Fig. 2A), and visualization with the Highlighter tool (http://www.hiv.lanl.gov) (Fig. 2B). Overall, the maximum nucleotide diversity of the env sequences from the SIVmac251 stock (UCD-6/04) used for this study was 1.0%, which is consistent with the (UCD-2/02) SIVmac251 stock11 and other SIVmac251 stocks.44 It is worth noting, although perhaps not surprising, that the env variants in the (UCD-6/04) SIVmac251 stock are closely related to the env variants in the (UCD-2/02) SIVmac251 stock (Fig. 2C).
A total of 94 SGA-derived 2.7-kb env sequences were obtained from the first vRNA+ plasma sample of five rhesus macaques (median of 20 sequences; range 16–20). SGAs were derived from the first plasma sample that had vRNA levels above the assay cutoff of 1.7 log10 copies/ ml plasma. These samples were collected 7 or 10 days after penile exposure to SIVmac251 (Table 1). Although many of the animals were exposed multiple times, we assumed that the exposure responsible for transmitting the infection occurred 7–10 days before the initial detection of viral RNA in plasma during the ramp-up phase of infection. This estimate of 7–10 days from the transmitting exposure was confirmed using the Poisson diversity tool (www.hiv.lanl.gov). The viral loads in these samples ranged from 2.42 to 4.19 log10 copies/ml of plasma (Table 1). Although no sequences were overtly APOBEC hypermutated as assessed using the Hypermut tool (www.hiv.lanl.gov), several sequences were enriched for G-to-A mutations and excluded from calculations of diversity and divergence from the SIVmac251 consensus reference sequence. The mean sequence diversity of each animal during acute infection ranged from 0.01 to 0.03% with an average for all animals of 0.02%. The maximum sequence diversity for each animal ranged from 0.07 to 0.15% with an average maximum diversity of 0.09%. For each infected animal, a single low-diversity lineage was detected in the ramp-up phase plasma.
In addition to ramp-up sequences, 76 SGA-derived 2.7-kB env sequences were obtained from set point vRNA+ plasma samples of the same five rhesus macaques (median of 17 sequences per animal; range 9–17). These sequences were derived from plasma samples that had vRNA levels above 5.0 log10 copies/ml plasma. These samples were collected 12–19 weeks after the presumed transmitting penile SIVmac251 exposure (Fig. 1). The mean sequence diversity of in these samples ranged from 0.1 to 0.2% with a mean of 0.18%. The maximum sequence diversity for each animal in these samples ranged from 0.3 to 0.6% with an average maximum diversity of 0.4%. Overall, the total diversity correlates to the amount of time since infection.
To enumerate the total number of transmitted/founder variants, the number of distinct low-diversity viral env lineages present in each sample was estimated. Low-diversity monophyletic lineages are defined as a unique series of identical or nearly identical nucleotide sequences, differing only by the number of individual polymorphisms that could arise randomly through RT error within 7 days of transmission. In this study, we define a variant as an SGA-derived genetic sequence with up to two individual nucleotide polymorphism differences from another variant assuming no recombination or selection pressure, and a gamma distributed rate of mutation across sites. The maximum number of nucleotide mutations likely to occur by random error within the time frame from virus exposure has been determined mathematically. A 2.2- to 2.7-kb fragment of SIV env will accumulate a maximum of two nucleotide polymorphisms in 1 week of SIV infection. Thus if a plasma SGA sequence had more than two nucleotide substitutions relative to the consensus sequence of each lineage, then the variant is assumed to have been transmitted as distinct founder virus.
Based on these criteria, a single SIV env variant was detected in the ramp-up plasma of all five SIV-infected animals (Fig. 3). A composite neighbor-joining tree, illustrating the phylogenetic relationship of all SIV env sequences derived from the SIV stock and all five animals, demonstrates that the SIV env variants transmitted to the animals were widely distributed among the env variants in the stock with no overrepresentation of any specific env lineage in the stock (Fig. 3). Furthermore, the set point phase variants in plasma clearly arose from the founder variants detected at week 1 PI (Fig. 3) with no evidence of the emergence of novel variants that could have been missed in the week 1 PI plasma. This result supports the conclusion that each of the five animals was infected with a single SIVmac251 env variant that then gave rise to all subsequent viral variants detected at the set point stage of infection.
This study demonstrates that SIV can be transmitted to male rhesus macaques by immersing the glans and shaft of the penis in a suspension of cell-free virions. Although penile transmission of SIV is possible, it is not as efficient as vaginal SIV transmission. For example, when exposed to an SIVmac251 inoculum containing 103 TCID50, four males resisted infection despite 7–14 penile exposures, while seven of eight female macaques became infected after 14 vaginal exposures and all eight were infected by 16 challenges.10 Furthermore, 21 of 21 female rhesus macaques became infected after two vaginal challenges (separated by a 4h interval) in a single day with 105 TCID50 of SIVmac251,9 while only three of five male macaques became infected after penile exposure to the same inoculum and schedule (Table 1, Fig. 1). The relative inefficiency of penile SIV transmission is consistent with the epidemiologic studies in stable HIV-discordant couples that concluded that women are more susceptible to acquiring HIV per heterosexual act than men.27–29,66 The reasons for the difference in gender susceptibility remain to be determined, but as shown here these differences persist even if the doses of virus to which the genders are exposed are the same. It seems likely that differences in the relative surface area of the genital mucosa (the surface area of the vaginal mucosa is much greater than the surface area of the glans and foreskin), the relative density of susceptible target cells in the genital mucosa of men and women, and the time that the inoculum is in contact with the genital mucosa contribute to the differences in the efficiency of HIV transmission between the genders.
In heterosexual men, the presence of an intact foreskin is associated with an increased risk of HIV acquisition30,34–36,67,68 and the efficiency of penile SIV transmission would likely increase if an effort was made to directly inoculate the foreskin mucosa. In addition, HIV transmission to men is dramatically increased by the presence of genital ulcers69; thus experimentally inducing penile inflammation prior to SIV inoculation may be another strategy to enhance penile SIV transmission efficiency while also modeling populations at greatest risk of acquiring HIV.
Transmission of HIV is relatively inefficient compared to other sexually transmitted infections (STI), which is reflected in the fact that in most cases a single variant or only a few variants from the viral quasispecies in an HIV-infected person successfully establishes systemic infection.70–73 As systemic infection with a single variant occurred in five of five animals after penile SIVmac251 transmission, this route of experimental SIV inoculation accurately reflects the virology of HIV transmission. In fact, when infection is transmitted, single SIV env variants establish systemic infections more consistently after penile SIVmac251 exposure compared to vaginal SIVmac251 challenge. After vaginal challenge of randomly selected mature cycling multiparous rhesus macaques with a complex SIVmac251 quasispecies11,17 some animals became infected with one variant, but in about half of the animals, systemic infection was established by five or more unique variants.11 The transmission of multiple variants to some female rhesus may be due to the changes in physiology of the female reproductive tract during the menstrual cycle or the presence of vaginal inflammation at the time of inoculation. Although ulcerative STIs have not been described in macaques, female rhesus monkeys commonly have bacterial vaginosis74,75 that causes genital inflammation.76 Alternatively, the same factors (discussed above) that may make women relatively more susceptible to HIV infection may also increase the number of variants that are transmitted by vaginal SIV inoculation. It remains to be seen if experimentally induced inflammation alters the number of variants transmitted by penile SIV inoculation.
The genetic bottleneck that results in a single viral variant establishing an infection after HIV transmission43,52,70,71,77–79 could be due to a the presence of only a limited number of viral variants in secretions of the infected partner, selective transmission of viral variants, or selective amplification of viral variants that cross the mucosal barrier. Although there could be a reduction in overall diversity in mucosal secretions compared to plasma, this penile infection study is not consistent with first explanation since all five animals inoculated with the genetically diverse SIVmac251 quasispecies became infected with a unique, single virus variant. It should be noted that analyzing 20 SGA-derived sequences provides a 95% confidence estimate that variants representing greater than 15% of the population are not be missed.43 Thus while the most likely conclusion is that all five animals were infected with a single variant we cannot exclude the possibility an individual animal has more than one variant in the plasma sample tested.
Despite this caveat, this is now the fourth study to reach the conclusion that rhesus macaques became infected with a single env variant after mucosal SIVmac251 exposure; the other studies tested animals after vaginal and rectal SIVmac 251 inoculation.11,17,56 Further, because all variants at set-point were derived from the variants we identified at ramp-up, all biologically relevant variants seem to have been found with our approach. Determining the relative importance of selective transmission versus selective amplification of a single variant prior to systemic dissemination will be important for understanding the challenges associated with preventing HIV transmission through vaccination. In some HIV clades, the transmitted HIV env variants share features that presumably enhance transmission to a seronegative host, but they also make the transmitted variants relatively susceptible to antibody neutralization.80 In this case preexisting antibodies may be able to efficiently block infection by the founder viruses. On the other hand, if the neutralization-sensitive variants appear as the founder population in blood by outcompeting less fit viruses for limited target cells in the first few days after transmission, then a neutralization-resistant variants may emerge as the primary founder viruses in the presence of established vaccine-induced neutralizing antibodies.
Finally, although infection is established relatively inefficiently by penile SIV inoculation, these data are consistent with the epidemiology of HIV transmission in human populations and represent the first use of this route in NHP models. Even after penile inoculations with a high virus dose the pattern of viremia and the single variant founder SIV populations that establish infection accurately reflect the virology of HIV infection. Given the consistency of infection, this route of SIV inoculation can be used to model penile HIV transmission to better understand virus transmission and dissemination. Furthermore, this route of virus challenge will be very useful for accurately modeling HIV transmission in the preclinical testing of HIV vaccines.
The authors thank the Primate Services Unit at the CNPRC and Joseph Dutra, Ding Lu, and Lili Guo for excellent technical assistance. This work was supported by NIH Grants RR00169 and AI8227, National Cancer Institute contract no. HHSN266200400088C, and a gift from the James B. Pendleton Charitable Trust.
No competing financial interests exist.