Reporter bacteriophage A511::celB transduces a hyperthermostable glycosidase from Pyrococcus furiosus for rapid and simple detection of viable Listeria cells

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Published online 2011 May 1. doi: 10.4161/bact.1.3.16710

Copyright/LicenseRequest permission to reuse: Copyright © 2011 Landes Bioscience

Table 2

Listeria detection in artificially contaminated smoked salmon and chocolate milk

Assay variables	Salmon		Chocolate milk
Assay variables	1 cfu/g	10 cfu/g	1 cfu/g	10 cfu/g
24 h selective enrichment/2.5 h non-selective enrichment MUG assay 180 min	−	−	−	+
24 h selective enrichment/2.5 h non-selective enrichment MUG assay 360 min	−	+	+	+
24 h selective enrichment/5.5 h non-selective enrichment MUG assay 180 min	−	−	+^a	+^a
24 h selective enrichment/5.5 h non-selective enrichment MUG assay 360 min	−	−	+^a	+^a
48 h selective enrichment/5.5 h non-selective enrichment MUG assay 180 min	−	+	+	+
48 h selective enrichment/5.5 h non-selective enrichment MUG assay 360 min	−	+	+	+

^aSignal-to-noise ratio increased significantly compared to values obtained after 2.5 h of incubation.

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