Search tips
Search criteria 


Logo of mabsLink to Publisher's site
MAbs. 2011 Jul-Aug; 3(4): 362–375.
Published online 2011 July 1. doi:  10.4161/mabs.3.4.16090
PMCID: PMC3218533

Development of an Fn14 agonistic antibody as an anti-tumor agent


TNF-like weak inducer of apoptosis (TWEAK), a tumor necrosis factor (TNF) family ligand with pleiotropic cellular functions, was originally described as capable of inducing tumor cell death in vitro. TWEAK functions by binding its receptor, Fn14, which is upregulated on many human solid tumors. Herein, we show that intratumoral administration of TWEAK, delivered either by an adenoviral vector or in an immunoglobulin Fc-fusion form, results in significant inhibition of tumor growth in a breast xenograft model. To exploit the TWEAK-Fn14 pathway as a therapeutic target in oncology, we developed an anti-Fn14 agonistic antibody, BIIB036. Studies described herein show that BIIB036 binds specifically to Fn14 but not other members of the TNF receptor family, induces Fn14 signaling and promotes tumor cell apoptosis in vitro. In vivo, BIIB036 effectively inhibits growth of tumors in multiple xenograft models, including colon (WiDr), breast (MDA-MB-231) and gastric (NCI-N87) tumors, regardless of tumor cell growth inhibition response observed to BIIB036 in vitro. The anti-tumor activity in these cell lines is not TNF-dependent. Increasing the antigen-binding valency of BIIB036 significantly enhances its anti-tumor effect, suggesting the contribution of higher order cross-linking of the Fn14 receptor. Full Fc effector function is required for maximal activity of BIIB036 in vivo, likely due to the cross-linking effect or tumor killing activity caused by antibody-dependent cell-mediated cytotoxicity. Taken together, the anti-tumor properties of BIIB036 validate Fn14 as a promising target in oncology and demonstrate its potential therapeutic utility in multiple solid tumor indications.

Key words: TWEAK, Fn14, monoclonal antibody, agonist, xenograft, apoptosis


The tumor necrosis factor (TNF) superfamily represents an attractive opportunity for therapeutic targeting in cancer because of its tumor cell killing activity. A number of TNF family members, including TNF and Fas/Apo1, have been evaluated in clinical studies, but toxicities related to systemic exposure have severely limited their development as cancer therapies, although alternative strategies for targeted or local delivery are still being pursued.1 More recently, targeting of other TNF family members, including TNF-related apoptosis inducing ligand (TRAIL/Apo2L) and CD40, have emerged as promising therapeutic approaches.1,2 Notably, recombinant soluble TRAIL and agonist antibodies to the TRAIL receptors, TRAIL-R1 (death receptor (DR)4) and TRAIL-R2 (DR5), which exhibited impressive efficacy in tumor xenograft models, are currently undergoing early clinical testing with encouraging results regarding safety and tolerability.3

TNF-like weak inducer of apoptosis (TWEAK) and its receptor, FGF-inducible molecule 14 (Fn14), are members of the TNF superfamily. Like TNF, TWEAK is a type II transmembrane protein which forms homotrimers that can function as soluble cytokine upon cleavage from the cell surface. TWEAK is a pleiotropic factor with a broad range of biological capabilities, such as pro-inflammatory activity and promotion of angiogenesis, migration, invasion and survival.4 TWEAK was initially described and named for its ability to weakly induce HT29 tumor cell killing in vitro,5 typically requiring co-incubation with sensitization agents such as IFNγ.6

While Fn14 is generally expressed at relatively low levels on normal tissues, elevated Fn14 expression is observed in settings of tissue injury and regeneration,710 and, notably, in tumors including breast, pancreatic, esophageal and glioma.7,1115 In the largest survey to-date examining 1,655 tumor samples across 22 solid tumor subtypes by immunohistochemistry, Fn14 expression was detected in the majority of tumor types, including pancreatic cancer (60%), non-small cell lung cancer (55%), bone metastases (54%) and liver metastases in colorectal cancer (50%).16 A significant correlation between increased Fn14 expression and higher tumor grade or poor prognosis has been documented in glioma, breast cancer and esophageal cancer.14,15,17

Upon engaging TWEAK, the intracellular domain of Fn14 recruits TNF receptor associated factor (TRAF) molecules and induces signaling through nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB) and mitogen-activated protein kinases pathways.18,19 NFκB pathway stimulation by TWEAK/Fn14 has been documented in numerous contexts,4 with evidence of stimulation of both canonical and non-canonical signaling.18,19 Although the scavenger receptor CD163 has been proposed to be an alternative receptor for TWEAK,20 the biological implications of this interaction are unknown. Notably, tumor cell death induced by TWEAK is thought to be solely mediated through Fn14.21

Unlike many other TNF family receptors with death-inducing activity, Fn14 does not contain a death domain, and consequently the mechanism by which TWEAK induces cell death is not well understood. In fact, there appear to be multiple mechanisms by which TWEAK can induce tumor cell death, and in some cases cell death induced by TWEAK may be mediated through other pathways. For example, TWEAK-induced cell death of some tumor cell lines, such as Kym-1, SKOV-3 and OVCAR, is TNF-dependent and involves recruitment of TRAF2 and cIAP-1 degradation.2224 On the other hand, TWEAK-induced cell death of tumor cells such as HSC3, HT-29 and KATO-III is independent of TNF.22 Similarly, TWEAK-induced cell death can be caspasedependent or caspase-independent, with features of both apoptosis and cathepsin-B dependent necrosis.21,25

To date, the anti-tumor activity of TWEAK has been exclusively demonstrated in vitro. Herein, we show that in vivo administration of TWEAK is efficacious in inhibiting tumor growth in a xenograft model. To exploit the tumor cell death inducing capacity of the TWEAK-Fn14 pathway, we developed an anti-Fn14 agonistic antibody, BIIB036, which mimics the signaling activity of TWEAK and can stimulate tumor cell apoptosis. BIIB036 exhibits potent anti-tumor activity in multiple xenograft models, despite its variable tumor cell inhibition activity in vitro. Interestingly, tumor cell killing in these cell lines does not appear to be TNF-dependent. The anti-tumor activity of BIIB036 is enhanced by increasing antigen-binding valency, indicating a requirement for Fn14 receptor cross-linking to maximize activity. BIIB036 can also induce antibody-dependent cell-mediated cytotoxicity (ADCC) in vitro. Consistent with these observations, we found that full Fc effector function is required for maximal activity of BIIB036 in xenograft models, likely reflecting the contribution of Fc receptors in mediating Fn14 cross-linking or ADCC activity.


Administration of TWEAK is efficacious in a breast tumor xenograft model.

TWEAK has been shown to induce tumor cell death in vitro.5,22,23 To determine whether in vivo delivery of TWEAK can inhibit tumor growth, adeno-TWEAK (or control adeno-GFP) was directly injected into MDA-MB-231 human breast tumors grown in nude mice. As shown in Figure 1A, the average tumor volumes were statistically different (p < 0.007) between the adeno-TWEAK treated groups and control (adeno-GFP) from day 23 through the end of the study (day 76). At day 76, tumors from the adeno-TWEAK treated mice had an 82% reduction in volume compared with tumors from the adeno-GFP treated group. Figure 1B shows the actual tumor weights determined upon dissection at termination of the study, confirming a significant reduction in tumor mass in the adeno-TWEAK treated group (Fig. 1B). Notably, upon dissection at day 76, two of the seven mice in the adeno-TWEAK treated groups had no discernable tumor mass. Upon histological examination, the adeno-TWEAK treated tumors exhibited reduced density of tumor cells and decreased levels of proliferation as evident by H&E and Ki-67 staining, respectively (Fig. 1C). Consistent with this, increased levels of apoptotic cells as assessed by TUNEL staining was observed in the adeno-TWEAK treated group (Fig. 1C). Thus, intratumoral adeno-TWEAK administration was efficacious in reducing tumor growth in a xenograft model.

Figure 1
Adeno-TWEAK or Fc-TWEAK treatment is efficacious in tumor xenograft model. (A) Efficacy of adeno-TWEAK treatment in MDA-MB-231 xenograft model is shown. MDA-MB231 cells were grown subcutaneously in nude mice and a single dose of adeno-TWEAK or adeno-GFP ...
Table 1
Effect of BIIB036 on metastasis in MDA-MB-231 breast tumor model

To verify that TWEAK is indeed responsible for the observed anti-tumor effect, we also tested whether administration of soluble Fc-TWEAK, in the absence of the viral delivery system, would be efficacious in the xenograft model. MDA-MB-231 tumor bearing mice were treated bi-weekly with intratumoral administration of Fc-TWEAK (or vehicle control). Figure 1D shows that the average tumor volume in the Fc-TWEAK treated mice was statistically smaller (p < 0.02) compared to tumors in control treated mice from day 54 through termination of the study (day 96). Figure 1E shows that on average there was an 86% reduction in actual tumor weight upon dissection at termination of the study (Fig. 1E). Taken together, these results demonstrate that TWEAK can inhibit tumor growth in vivo, suggesting a potential therapeutic opportunity to exploit the pathway for the treatment of cancer.

Development of an agonistic anti-Fn14 antibody, BIIB036.

The studies described above provided evidence of the in vivo tumor cell killing capacity of TWEAK. However, as a therapeutic agent, an adenoviral delivery system was not considered a viable option. Fc-TWEAK was also not a feasible approach given its complex oligomeric structure resulting from the dimerizing capacity of the Fc domain coupled with the trimerizing region of TWEAK (Hsu Y-M, unpublished results). Since Fn14 is the sole receptor for TWEAK with biological relevance and is responsible for mediating tumor cell killing,21 we chose to develop an agonistic anti-Fn14 monoclonal antibody as a candidate therapeutic agent to target the pathway.

The murine antibody mBIIB036 (P4A8)14 was selected from a panel of anti-Fn14 antibodies that was generated in Fn14 knockout mice.8 We demonstrated that mBIIB036 binds specifically to Fn14, but not to other members of the TNF receptor family (Sup. Fig. 1). mBIIB036 was subsequently humanized by CDR grafting29 to generate a drug candidate, BIIB036, with a human IgG1 backbone. The binding affinity of BIIB036 to human monomeric Fn14 as measured by Biacore is 1.7 nM (Sup. Fig. 2), a value indistinguishable from that calculated for the murine version of the antibody (mBIIB036). To evaluate whether BIIB036 is capable of binding Fn14 expressed on the cell surface, FACS analysis was performed. Figure 2A shows that BIIB036 binds to 293E cells transiently transfected with full length human Fn14, but not to 293E cells transfected with empty vector. In addition, Figure 2A shows similar binding affinities of BIIB036 to 293E cells transfected with mouse, rat or cynomolgus monkey Fn14 (Fig. 2A). We also characterized the binding site of BIIB036 by competitive binding of TWEAK versus BIIB036 to cell surface Fn14 in the WiDr colon carcinoma cell line. Figure 2B shows that binding of TWEAK to Fn14 can be blocked by BIIB036 in a dose-dependent fashion. Likewise, binding of BIIB036 can be blocked by TWEAK (data not shown). Thus, BIIB036 binds Fn14 with high affinity and specificity, binds Fn14 across species, and is capable of reciprocally competing with TWEAK for binding to Fn14.

Figure 2
BIIB036 binds Fn14 and blocks TWEAK binding to Fn14. (A) Direct FACS binding assay of BIIB036 to 293E cells transiently transfected with full-length Fn14 from various species is shown. An empty vector (“control”) was included as a negative ...

Agonistic activity was a key requirement for the anti-Fn14 antibody and a basis upon which BIIB036 was selected from the panel of antibodies. We first tested BIIB036 agonistic activity in a chemokine release assay. Figure 3A shows that mBIIB036 is capable of inducing IL-8 release in A375 cells, albeit significantly less potently compared with Fc-TWEAK. Similar results were observed with measurement of MCP-1 and IP-10 release in A375 cells (data not shown). In addition, we evaluated signaling through the non-canonical NFκB pathway, as has previously been described for TWEAK,18,19 by monitoring p100 cleavage to p52. We found that treatment of WiDr or MDA-MB-231 tumor cells with BIIB036 resulted in the appearance of p52 (Fig. 3B), similar to the effect observed upon treatment with Fc-TWEAK (Fig. 3B). Canonical NFκB pathway signaling was also observed in response to BIIB036 treatment (data not shown). Taken together, the data indicated that BIIB036 exhibits agonistic activity, including induction of chemokine release and NFκB signaling, analogous to the effects of TWEAK.

Figure 3
BIIB036 exhibits agonistic activity. (A) IL-8 release assay in A375 cells is shown. Supernatants from A375 cells incubated for 36 h with mBIIB036, but not control Ig, showed increased levels of IL-8, as measured by ELISA, in a dose-dependent fashion. ...

BIIB036 can induce tumor cell death in vitro.

Next, the ability of BIIB036 to inhibit tumor growth in vitro was examined. BIIB036 and Fc-TWEAK were evaluated in an MTT assay on a panel of 38 human tumor cell lines (breast, colon, gastric, pancreatic, ovarian, non-small cell lung, head/neck, melanoma and renal). A range of sensitivity was observed in the various cell lines. Approximately 50% of the cell lines showed growth inhibition in response to BIIB036. All of the BIIB036-sensitive cell lines also exhibited growth inhibition in response to Fc-TWEAK. However, cell lines were typically more sensitive to Fc-TWEAK compared with BIIB036, with approximately half of the BIIB036-insensitive lines showing sensitivity to TWEAK. Moreover, Fc-TWEAK was typically more potent than BIIB036 in inhibiting tumor growth in cell lines that were sensitive to both agents. Of note, there was no correlation between sensitivity to tumor cell killing and the relative levels of Fn14 expression (Amatucci A and Michaelson JS, unpublished results), similar to the case of TRAIL receptor expression not correlating with sensitivity to anti-TRAIL antibodies.2

WiDr was identified as one of the most highly sensitive cell lines in the MTT assays. Figure 4A shows that both BIIB036 and Fc-TWEAK can inhibit WiDr cell growth, although BIIB036 is significantly less potent than Fc-TWEAK. To confirm that the inhibition of tumor growth observed in the MTT assay was an apoptotic effect, a TUNEL assay was performed. Figure 4B shows that BIIB036 and Fc-TWEAK treated cells are positive for TUNEL staining. This effect was specific, as the positive staining was abrogated when WiDr cells were treated with BIIB036 or Fc-TWEAK in the presence of soluble Fn14 (data not shown). Compared with WiDr cells, many other cell lines were less sensitive to Fn14 pathway stimulation. NCI-N87 gastric tumor cells (Fig. 4C), exhibited modest tumor cell growth inhibition in response Fc-TWEAK, but the cells were not sensitive to BIIB036. MDA-MB-231 breast cancer cells were similarly modestly sensitive to Fc-TWEAK, but not growth inhibited in response to BIIB036 (Fig. 4D). Notably, all three cell lines (WiDr, NCI-N87 and MDA-MB-231) showed relatively similar levels of Fn14 cell surface expression as measured by flow cytometry (data not shown). Taken together, our results indicate that the degree of sensitivity to Fn14 pathway stimulation varies among tumor cell lines, and typically cells are more sensitive to TWEAK compared with BIIB036.

Figure 4
BIIB036 can induce tumor cell killing in vitro. (A) MTT assay was performed with WiDr cells treated with a range of doses of Fc-TWEAK, BIIB036 or human Ig control antibody in the presence of IFNγ for four days. Results are graphed as percent survival ...

Multimerization of BIIB036 enhances anti-tumor activity in vitro.

Because Fc-TWEAK consistently exhibited more potent tumor inhibition compared to BIIB036, we investigated whether increasing the Fn14-binding valency might enhance the activity of BIIB036. We generated a multimeric version of BIIB036 (BIIB036-multimer) by complexing the antibody with soluble protein A; the multimer was purified by size exclusion chromatography to remove uncomplexed components (Sup. Fig. 3). The tumor inhibition activity of BIIB036-multimer was compared to that of BIIB036 and Fc-TWEAK in a panel of 24 cell lines. In the majority of cell lines tested, BIIB036-multimer exhibited enhanced inhibition of tumor cell growth relative to BIIB036; in fact, BIIB036-multimer showed activity in >90% of the cell lines that were sensitive to Fc-TWEAK. Although more potent than BIIB036, BIIB036-multimer nevertheless exhibited less potent anti-tumor activity compared with Fc-TWEAK. The enhanced anti-tumor activity of BIIB036-multimer relative to BIIB036 in WiDr cells is shown in Figure 5. In the NCI-N87 cell line, which was insensitive to BIIB036, BIIB036-multimer was modestly active, exhibiting slightly less potent activity compared with Fc-TWEAK (Fig. 4B). In the MDA-MB-231 cell line, where BIIB036 also had no anti-tumor activity, BIIB036-multimer was only minimally active (Fig. 4C).

Figure 5
Multimerization of BIIB036 results in enhanced anti-tumor activity. MTT assay was performed with WiDr cells in the presence of IFNγ treated with a range of doses of BIIB036 or control Ig, in the presence or absence of a secondary cross-linking ...

The increased activity of BIIB036-multimer suggested that in an in vivo context, interaction of the BIIB036 Fc domain with an array of Fc receptors on the cell surface of immune cells might lead to oligomeric structures and result in enhanced anti-tumor activity. To more closely mimic Fc engagement by Fc receptors, we evaluated BIIB036 activity in the presence of a cross-linking secondary antibody. As shown in Figure 5, WiDr tumor growth inhibition by BIIB036 is increased under these conditions. Notably the enhanced activity of the cross-linked BIIB036 is comparable to that of BIIB036-multimer. In contrast, no activity was observed upon cross-linking of a control Ig. Of note, no tumor inhibition was observed in response to a monomeric Fab fragment derived from BIIB036 (data not shown). Thus, oligomeric BIIB036, generated either by secondary antibody-mediated cross-linking or by complexing via protein A, exhibited enhanced anti-tumor activity in vitro.

Anti-tumor activity is TNF-independent in most cell lines.

As it has been reported that tumor cell killing induced by TWEAK in various cell lines (e.g., Kym-1, SKOV-3, OVCAR) is mediated by TNF,23,24,30 we evaluated whether cell killing induced by TWEAK, BIIB036-multimer or BIIB036 is TNF dependent in a panel of 12 tumor cell lines. TNF dependence was determined by performing the MTT assay in the presence or absence of the anti-TNF antibody, adalimumab (500 pM), which was previously shown to effectively inhibit TNF at concentrations up to 200 pM (data not shown). Interestingly, no diminution in tumor cell inhibition was observed when the MTT assay was performed in the presence of adalimumab in the majority (9/12) of cell lines tested, suggesting that TNF is not required for TWEAK-induced killing in these cell lines. An example of a TNF-independent cell line, WiDr, shows growth inhibition induced by Fc-TWEAK, BIIB036-multimer or BIIB036 is comparable in the presence or absence of adalimumab (Fig. 6A). NCI-N87 and MDA-MB-231 were also TNF-independent cell lines (data not shown). In contrast, adalimumab was effective in inhibiting TWEAK-mediated killing in SKOV-3 ovarian tumor cells (Fig. 6B), a cell line previously reported to be TNF-dependent.24 We could detect TNF in supernatants and cell extracts of SKOV-3 cells treated with Fc-TWEAK (data not shown), consistent with the previously reported effect of TWEAK in this cell line.24 However, TNF was not detectable in supernatants or cell extracts prepared from other cell lines treated with Fc-TWEAK (data not shown). Interestingly, while TWEAK-induced growth inhibition in Panc-1 cells was TNF-dependent in the MTT assay, soluble TNF was not detectable in supernatants or cell extracts prepared from Panc-1 cells treated with TWEAK (data not shown). Taken together, our findings demonstrate that TWEAK-mediated cell killing is TNF independent in a majority of cell lines tested, including WiDr, NCI-N87 and MDA-MB-231.

Figure 6
Cell killing in multiple tumor cell lines is TNF independent. MTT assays in (A) WiDr cells and (B) SKOV-3 cells were performed in the presence or absence of the anti-TNF inhibitor, Adalimumab (500 pM). Cell survival is plotted as a function of concentration ...

BIIB036 exhibits ADCC activity in vitro.

Given that the Fc portion of BIIB036 is a human IgG1, we reasoned that, in addition to effecting cross-linking, the Fc portion of BIIB036 might also contribute to anti-tumor activity by inducing ADCC or CDC. To test this hypothesis, we generated variants of BIIB036 with aglycosylated Fc ends to ablate effector function,31 including aglycosylated hIgG1 (hIgG1agly) with reduced effector function and aglycosylated IgG4 variant (hIgG4Pagly) with no effector function. To assess ADCC activity in vitro, a chromium release assay was performed in the presence of NK effector cells. As shown in Figure 7A and B, ADCC activity was observed in MDA-MB-231 and WiDr tumor cells treated with BIIB036. In contrast, the Fc crippled variants (hIgG1agly and hIgG4Pagly) did not exhibit activity in these assays, as expected (Fig. 7A and B). We also performed CDC assays. No CDC activity was observed with BIIB036 in multiple cell lines tested including WiDr and MDA-MB-231 (data not shown). Thus, an in vitro evaluation of Fc effector function revealed potential ADCC, but not CDC activity of BIIB036.

Figure 7
BIIB036 exhibits ADCC activity in vitro. ADCC assays were performed in (A) MDA-MB-231 and (B) WiDr cells with a 5:1 ratio of NK to target tumor cells in the presence of increasing concentrations of antibody. BIIB036 (hIgG1) was tested and compared to ...

BIIB036 is efficacious in multiple xenograft models.

Having established that BIIB036 can inhibit tumor cell growth in vitro, we next tested whether it was efficacious in tumor xenograft models in vivo. We first chose to evaluate BIIB036 in the WiDr colon carcinoma model, since WiDr was among the most sensitive cell lines in vitro. Mice bearing WiDr tumors with an average volume of 250 mm3 were treated intraperitoneally (IP) with BIIB036 at doses ranging from 0.9–12.8 mg/kg, or an irrelevant human IgG antibody (12.8 mg/kg), once a week for six weeks. As shown in Figure 8A, BIIB036 exhibited significant tumor growth inhibition at all doses examined (p < 0.001 compared to control from days 25–61). A dose response was observed, with maximal efficacy achieved at 6.4 and 12.8 mg/kg. Maximal tumor inhibition in the 6.4 mg/kg group relative to control IgG treated mice was 94% on day 61. Notably, at doses of 3.2 mg/kg and higher, BIIB036 appeared to induce tumor regression. We also monitored body weights over the course of the study, and BIIB036 was well tolerated with no reduction in body weight observed in any of the treatment groups (Fig. 8B).

Figure 8
BIIB036 inhibits tumor growth and induces apoptosis in WiDr colon tumor xenograft model. (A) Efficacy of BIIB036 treatment in WiDr colon carcinoma xenograft model is shown. Tumor volume as a function of time in WiDr xenograft model is plotted. Nude mice ...

To understand whether the growth inhibition of BIIB036 was associated with induction of apoptosis in vivo, tumor samples were taken 24 and 48 h after BIIB036 treatment (6.4 mg/kg) and evaluated for caspase-3 cleavage. Substantial staining of cleaved caspase-3, indicative of caspase-3 activation, at both time-points was evident (Fig. 8C). We continued to follow the levels of cleaved caspase-3 over time and found a return to baseline by day 7 post-treatment (Fig. 8D). Following a second dose of BIIB036, another wave of caspase cleavage was observed, with significant induction at the 24 and 48 h time points (data not shown).

We next evaluated BIIB036 in the NCI-N87 gastric carcinoma xenograft model where in vitro results indicated a lack of sensitivity to the antibody, but modest sensitivity to BIIB036-multimer. Although NCI-N87 tumor cells were not sensitive to BIIB036, the in vitro sensitivity to BIIB036-multimer suggested that the potential interaction of BIIB036 with Fc receptors on immune effector cells might facilitate generation of multimeric forms of BIIB036 thereby promoting anti-tumor activity in vivo. Established NCI-N87 tumor (250 mm3) bearing mice were treated with BIIB036 (at 3.2, 6.4 or 12.8 mg/kg intraperitoneally) or an irrelevant human IgG antibody (12.8 mg/kg) on a weekly dosing schedule for 6 weeks. BIIB036 treatment significantly inhibits tumor growth compared with control antibody (p < 0.01 compared to control from days 21–54), with no dose-dependency at the doses evaluated (Fig. 9A). Maximal tumor inhibition in the 6.4 mg/kg dose group compared with the control group was 77% on day 54. Thus, despite the reduced activity observed in vitro in NCI-N87 cells, BIIB036 exhibited potent anti-tumor efficacy in the NCI-N87 xenograft model.

Figure 9
BIIB036 inhibits tumor growth in NCI-N87 gastric and MDA-MB-231 breast tumor xenograft models and reduces lymph node metastases. (A) Efficacy of BIIB036 treatment in NCI-N87 gastric xenograft model is shown. Nude mice implanted with NCI-N87 tumor cells ...

Finally, we tested the efficacy of BIIB036 in a xenograft model using a breast tumor cell line, MDA-MB-231, which lacked in vitro sensitivity to BIIB036 and was only marginally sensitive to BIIB036-multimer. MDA-MB-231 tumor-bearing mice were treated once weekly for six weeks with BIIB036 at 6.4, 12.8 or 25.6 mg/kg or an irrelevant human IgG antibody (25.6 mg/kg).As shown in Figure 9B, significant tumor inhibition was observed at all doses tested. In the 6.4 mg/kg group, maximal tumor inhibition compared to the control group was 74% on day 70. Notably, caspase-3 cleavage was observed in the BIIB036-treated tumors (data not shown). Thus, significant tumor inhibition was observed following BIIB036 treatment despite the lack of anti-tumor activity observed in MDA-MB-231 cells in vitro.

Since MDA-MB-231 cells grown subcutaneously in the flank region metastasize to the lymph nodes, we had the opportunity to assess whether BIIB036 might affect the incidence or size of metastases. Treatment with BIIB036 reduced the incidence of gross metastases to the axillary lymph nodes compared with control Ig (40 versus 100%, respectively; Table 1). The metastatic incidence was confirmed by a similar reduction in micrometastastic lesions, as determined by histological staining with cytokeratin (Table 1). In addition, Figure 9C shows that the average size of the metastatic lesion was dramatically reduced in the BIIB036-treated mice compared with the control group (5.5 mm3 versus 709.4 mm3, respectively, p < 0.03). Furthermore, the extent of metastases in the lymph nodes, as quantified by cytokeratin staining, was significantly reduced (7% versus 68.2%, p = 0.02; Table 1). Similar results showing a diminution in incidence and size of metastases were observed in mice treated with BIIB036 at doses ranging from 6.4–25.6 mg/kg (data not shown). In addition, a reduction in mitotic events was also recorded in BIIB036-treated mice (data not shown). Taken together, BIIB036 exhibited strong anti-tumor activity in vivo in multiple xenograft models, despite varying levels of sensitivity in vitro, and also demonstrated anti-metastatic activity in a lymph node metastasis model.

Fc effector function is required for maximal antitumor activity of BIIB036.

Based on our observations that BIIB036 multimerization can enhance anti-tumor activity in vitro and that BIIB036 can induce ADCC in vitro, and given the activity of BIIB036 in xenograft models grown from relatively insensitive cell lines, we posited that the Fc domain likely contributes to BIIB036 activity in vivo. To test this, we compared the in vivo anti-tumor activity of BIIB036 (hIgG1) to the Fc crippled versions (hIgG1agly and hIgG4Pagly).

Figure 10A shows that in the MDA-MB-231 xenograft model, BIIB036 (hIgG1) exhibited 75% tumor growth inhibition compared with the control Ig (p < 0.0001 on day 62), whereas the reduced effector hIgG1agly version exhibited only 57% inhibition of tumor growth (p < 0.001 compared to control Ig on day 62). Moreover, the hIgG4Pagly version, which completely lacks effector function, had further reduced tumor growth inhibitory activity of 29% compared to control (p < 0.05 on day 62). Similar results were observed at other doses tested, including 6.4 and 25.6 mg/kg (data not shown). Figure 10B shows that in the WiDr model, the effectorless hIgG4Pagly version similarly exhibited significantly reduced tumor inhibitory activity (48%, p < 0.05 compared to control on day 56) compared with BIIB036 (91%, p < 0.001 compared with control on day 56). Similar results were obtained in the WiDr model when the different Fc versions of BIIB036 were compared at other doses, such as 3.2 and 6.4 mg/kg (data not shown). Thus, results from xenograft models clearly indicate that the Fc domain of the antibody contributes significantly to the growth inhibitory activity of BIIB036 in vivo.

Figure 10
BIIB036 exhibits superior anti-tumor activity in vivo relative to versions of the antibody with reduced effector function. (A) MDA-MB-231 and (B) WiDr tumor xenograft model were used to compare the efficacy of different Fc versions of BIIB036. BIIB036 ...


The data presented herein validate the TWEAK/Fn14 pathway as a target in oncology. We show that administration of TWEAK in vivo is efficacious in inhibiting tumor growth in a xenograft model. To therapeutically target the pathway, we developed a humanized agonistic anti-Fn14 antibody, BIIB036, which mimics many activities of TWEAK, including the ability to inhibit tumor growth. Importantly, BIIB036 is highly efficacious in inhibiting tumor growth in multiple xenograft models irrespective of its relative activity in vitro in the various cell lines. We found that the anti-tumor activity of BIIB036 can be enhanced by multimerization of the antibody, suggesting an effect of higher order cross-linking of Fn14 molecules on the cell surface. Indeed, we observed that maximal activity in vivo is achieved only with full effector function of the antibody, suggesting the role of Fc receptors in scaffolding the antibody or ADCC activity in vivo.

BIIB036 was developed as an anti-Fn14 agonistic antibody to mimic the activity of the natural ligand, TWEAK. Indeed, BIIB036 can induce chemokine release, activate NFκB pathway signaling, and, importantly, stimulate tumor cell death. However, BIIB036, the most potent agonist in our panel of anti-Fn14 antibodies, is significantly less potent than TWEAK. Notably, other anti-Fn14 antibodies that have been described also show reduced potency relative to TWEAK.16,32 The decreased potency might be a function of the dimeric interaction of an antibody with the Fn14 receptor in contrast to TWEAK which presumably engages in a trimer-trimer interaction with Fn14. It is also possible that a particular geometrical configuration of Fn14 engagement is unique to TWEAK. Nevertheless, as a therapeutic, BIIB036 is highly efficacious in multiple xenograft models. Moreover, as a monoclonal antibody, BIIB036 is a better drug candidate than soluble TWEAK. The adenoviral system, while delivering abundant quantities of soluble TWEAK, is not feasible for human use. Fc-TWEAK, consisting of a dimerizing Ig Fc domain and a trimerizing extra-cellular domain of TWEAK, can potentially fold into several oligomeric forms as observed by size exclusion chromatography (Hsu Y-M, unpublished observations). The minimal structure of Fc-TWEAK is expected to be a hexamer consisting of two TWEAK trimers and three dimeric Ig Fc domains. This complexity and the presence of heterogeneous populations of Fc-TWEAK, make it a poor drug candidate. Recombinant soluble TWEAK would also not be a desirable drug candidate due to an extremely short half-life in vivo (Burkly LC, data not shown). Finally, BIIB036 may be a safer alternative because TWEAK administration in vivo has been associated with significant weight loss in mice (reviewed in ref. 33 and Michaelson JS unpublished observations), whereas no weight loss was observed in response to BIIB036 treatment even with weekly administration at a high dose of 25.6 mg/kg.

Fn14 is emerging as a therapeutic target for the treatment of cancer, joining other TNF family member receptors, such as the CD40 and TRAIL receptors, that are currently under investigation. Similar to the robust anti-tumor activity we observed in xenograft models in response to TWEAK or BII036 treatment, efficacy was observed with recombinant soluble TRAIL and agonistic antibodies to the TRAIL receptors, (TRAIL-R1 (DR4) and TRAIL-R2 (DR5)) in numerous tumor xenograft models.2 Ongoing clinical trials with soluble TRAIL and anti-TRAIL receptor antibodies have thus far demonstrated acceptable safety and tolerability in humans,3 thereby providing precedence for safety with systemic administration of TNF family agonists in man. However, one notable distinction between Fn14 and TRAIL receptors is that Fn14 does not contain a death domain which is responsible for DR4- and DR5-mediated assembly of the death inducing signaling complex (DISC).1 The differential mechanism of inducing tumor cell death, along with the distinct pattern of receptor expression in human tumors, may allow for a differentiated opportunity for Fn14 agonists in the clinic.

In the absence of a death domain in the cytoplasmic region of Fn14, it is less clear how stimulation of the Fn14 pathway leads to death of tumor cells. We showed that, like TWEAK, BIIB036 is capable of inducing NFκB pathway activation. An early report suggested that TWEAK-induced killing is TNF-dependent in Kym-1 cells.23 However, Nakayama et al.22 reported that TWEAK-induced death of HT-29, HS-C or KATO-III cells does not exhibit TNF dependence, so it appears that not all Fn14-mediated cell death is TNF-dependent. Our analysis showed that tumor cell killing induced by Fn14 agonists is not TNF dependent in a majority of tumor cell lines evaluated. Specifically, we showed that only three (SKOV-3, MCF7 and Panc-1) of 12 cell lines were TNF dependent. Interestingly, Vince et al. showed that TWEAK killing is TNF dependent in the three cell lines (Kym-1, SKOV-3 and OVCAR) that they tested, and TNF was detectable in the supernatants and cell extracts of TWEAK-treated cells. However, in our evaluation of six cell lines, we could only detect TNF in supernatants or cell extracts from SKOV-3-treated cells, suggesting that the cell lines described in Vince et al. may not be representative. Studies in several other cell types have also documented that TWEAK signaling is independent of TNF, including pro-inflammatory responses in synoviocytes, dermal fibroblasts and keratinocytes.34,35 Taken together, the results suggest that TWEAK signaling in general, and TWEAK-induced tumor cell killing in particular, may be predominantly independent of TNF.

We observed robust anti-tumor efficacy with BIIB036 in multiple xenograft models regardless of relative activity in vitro. Notably, in the NCI-N87 and MDA-MB-231 cell lines, BIIB036 exhibited negligible anti-tumor activity in MTT assays, yet in vivo a 75% reduction in tumor growth was observed. This lack of correlation between in vitro and in vivo sensitivity highlights the limitations of making predictions based on cell-based assays alone. Stromal cells, tumor architecture and host-tumor interactions likely contribute to the anti-tumor response in vivo. Indeed, one explanation for the enhanced activity that we observed in vivo with BIIB036 is the contribution of Fc effector function. Fc receptor expressing immune effector cells can bind the Fc portion of BIIB036 and may effectively induce ADCC, as we showed in the in vitro reconstituted ADCC assay. Moreover, binding of BIIB036 by Fc receptor expressing cells may result in effective multimerization of BIIB036 leading to higher order cross-linking of Fn14 molecules and thereby an enhanced anti-tumor effect. Indeed, we show that multimerization through protein A or secondary antibody cross-linking of BIIB036 resulted in significantly enhanced activity in MTT assays and signaling studies (Amatucci A and Michaelson JS, unpublished observations). This is consistent with a recent report36 showing that Fcγ receptors are capable of driving antibody-dependent signaling in target cells in the context of agonistic DR5 and CD40 antibodies, indicating that Fcγ receptor-dependent mechanisms contribute to the activity of antibodies directed against other TNF family member receptors as well. Our in vivo studies, demonstrating that effectorless versions of BIIB036 exhibited reduced activity in xenograft models, confirmed the contribution of Fc effector function to BIIB036 activity. Residual tumor inhibition was nevertheless observed with the fully effectorless versions of BIIB036 in xenograft models, suggesting that not all of the activity is Fc-mediated. Our data suggest that this is due to signaling through Fn14 on tumor cells (Michaelson JS, unpublished observations), although, given species cross-reactivity of BIIB036 to mouse Fn14, a direct effect of BIIB036 binding to non-tumor cells cannot be ruled out.

It is important to note that a monoclonal antibody targeting Fn14, PDL192 (Facet Biotech/Abbott), is currently undergoing evaluation in a Phase 1 clinical study. One difference between PDL192 and BIIB036 is that the binding affinity, as determined by Biacore, is five times greater for BIIB036 compared to that of PDL192.16 While it is possible that there may not be a direct correlation between binding affinity and activity, nevertheless there could be implications in terms of relative competition for Fn14 binding to TWEAK. Like BIIB036, PDL192 exhibited anti-tumor activity in multiple xenograft models.16 BIIB036 was administered at significantly lower doses and on a less frequent dosing schedule compared with PDL192, suggesting that BIIB036 may be a more potent anti-tumor agent. It is also likely that BIIB036 and PDL192 have different binding epitopes on Fn14. Indeed, a notable feature of BIIB036 is an ability to bind Fn14 across species, with equivalent affinity to rodent, monkey and human Fn14. Perhaps this is due to the fact that BIIB036 was selected from a panel of anti-Fn14 antibodies that were generated in Fn14-deficient mice, which would favor cross-reactivity to rodent Fn14. The species cross-reactivity is critical for drug development because it provides a path forward for informative toxicology studies in rodents and non-human primates.

In considering BIIB036 as a therapeutic for the treatment of cancer, it is important to note that high expression of Fn14 has been reported in multiple tumor types, including breast, pancreatic, glioma and non-small cell lung.7,1116 Likewise, our xenograft studies, in which we observed robust efficacy, also spanned a range of tumor types, including colon, breast and gastric. Moreover, upregulated Fn14 expression was specifically observed in liver and bone metastases,16 and indeed BIIB036 demonstrated impressive anti-metastatic activity in the MDA-MB-231 model. In several tumor types, including glioma, breast and esophageal, a significant correlation between Fn14 expression and tumor grade and/or prognosis has been noted.1315,17,37 The upregulated expression of Fn14 in tumors may be due to some of the other features of the TWEAK/Fn14 pathway, such as the ability to promote migration, invasion and survival.38 Interestingly, although these tumor promoting features, as well as a capacity to induce angiogenesis,9,10,3942 have been attributed to TWEAK, it is important to emphasize that no enhancement in tumor growth was observed following BIIB036 treatment in any xenograft models tested to date. Thus, the fact that Fn14 is expressed in a wide variety of cancer types, and particularly in more aggressive tumors, together with our observation of in vivo anti-tumor efficacy in multiple tumor types, suggest that BIIB036 could be efficacious in the treatment of a broad range of tumors, and specifically in patients with advanced disease who are most in need of effective therapy.

Materials and Methods

Cell lines and reagents.

WiDr cells (NCI) were grown in MEM Earle's supplemented with 10% FBS, 2 mM L-glutamine, 1x non-essential amino acids, 0.5 mM sodium pyruvate. MDA-MB-231 cells (NCI) were grown in MEM Earle's or RPMI-1640 supplemented with 10% FBS. NCI-N87 cells (ATCC) were grown in RPMI-1640 medium with 10% FBS.

BIIB036-multimer was made by combining 5.58 mg BIIB036 with 0.35 mg Protein A (Pierce) in PBS overnight at 4°C, followed by purification on a Superdex 200 column (GE Healthcare) by AKTA FPLC (GE Healthcare).

The human IgG4 heavy chain was stabilized by mutation of hinge Kabat residue 228 from Ser to Pro (hIgG4P).26 The hIgG1agly and hIgG4Pagly heavy chains were made by mutation of the CH2's N-linked glycosylation site Asn-Ser-Thr (Kabat residues 297–299): residue 299 from Thr to Ala. Site-directed mutagenesis was performed using Stratagene's Quikchange mutagenesis kit following the manufacturer's recommended protocols. Mutations were confirmed by sequencing the hBIIB036 heavy chain cDNA insert in the relevant expression vectors.

The control antibody (control Ig) used in these studies was IDEC151.27

FACS binding studies.

Full-length Fn14 cDNAs (human, cynomolgus monkey, rat and mouse) were engineered to remove extraneous 5′ and 3′ UTRs and add an identical optimized Kozak sequence, then were subcloned into pNE001, a fully sequence-confirmed pUC-based EBV expression vector derived from the Invitrogen expression vector pCEP4, in which heterologous gene expression is controlled by a CMV-IE promoter and an SV40 polyadenylation signal, but lacking the EBNA gene and the hygromycin resistance gene. The Fn14 expression vectors were co-transfected into 293E cells at a 1:1 molar ratio with an EBV expression vector carrying an EGFP reporter. Cells were transfected using Qiagen's Effectene reagent, following the manufacturer's recommended protocol. Cells were used in FACS at 2 days post-transfection, staining with a dilution titration series of BIIB036 (detected with PE-conjugated goat anti-human IgG secondary antibody) and gating on green EGFP-positive living cells.

The FACS competition experiment was performed by titrating the antibodies (starting at 100 nM) followed by the addition of equal volume of a static concentration of recombinant soluble TWEAK (40 ng/mL). This combination was incubated with WiDr cells. Detection was with a PE labeled goat anti-mouse-Fc (JacksonImmuno#115-116-071). The geometric mean was determined on ungated cells and plotted.

Chemokine release assays.

A375 (2 × 104) human melanoma cells were grown in 96-well plates in the presence of varying concentrations of Fc-TWEAK,12 mBIIB036 or control Ig. After 36 h, the supernatants were collected and IL-8 release was measured by ELISA using the human IL-8 DuoSet ELISA kit (R&D Systems).

Signaling studies.

Western blots were performed according to standard procedures. The following antibodies were used: NFκB-p52/p100 (Cell Signaling #4882) and GAPDH (Ambion #AM4300).

Tumor cell killing assays.

(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assays were performing by seeding cells in a 96-well plate in media containing 80 U/ml human IFNγ (Biogen Idec) along with a serial dilution of Fc-TWEAK,12 BIIB036-multimer, BIIB036 or control Ig in triplicate. In some wells, the anti-TNF inhibitor adalimumab (Abbott) was added at a concentration of 500 pM. Cells were incubated for 3–4 days and developed using Cell Titer 96 Aqueous One Solution Cell Proliferation Assay (Promega). The percentage survival was determined as follows: % survival = (OD of treated wells/average OD of the untreated wells) × 100. All samples were run in triplicate, and data is presented with standard deviation.

For cross-linking studies, 16 µg/mL goat anti-human IgG F(ab')2 (Jackson Immuno#109-006-098) was combined with an equal volume of 12 µg/mL BIIB036 followed by nine serial 1:3 dilutions of this mixture in a 96-well plate. This titration resulted in a 2× solution of a 1:4 (molar) crosslinker:BIIB036 ratio, which was added to cells 1:1 by volume as above.

For terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays, cells were grown in 10-centimeter plates and treated with Fc-TWEAK12 (50 ng/ml) or BIIB036 (500 ng/ml) in the presence of IFNγ for 48 h. The assay was performed using the In-Situ Cell Death Detection Kit, Fluorescein-TUNEL (Roche) according to the manufacturer's instructions.

ADCC assays.

An enriched population of natural killer (NK) cells was purified from human peripheral blood monocytes (PBMC) using biotin-conjugated antibodies and microbeads cocktails (Miltenyi Biotec NK Cell Isolation Kit, Auburn, CA). The NK cells were grown overnight at 3 × 106/mL in RPMI with 10% Fetal Bovine Serum (Hyclone) and 20 units/mL of recombinant human interleukin-2 (IL-2) (Invitrogen). Target cells (WiDr or MDA-MB-231) were labeled with 150 µCi of 51Cr (Amersham Pharmacia Biotech) per 1 × 106 cells for 1 h at 37°C. The cells were washed four times, and then incubated in triplicate in 96-well plates in the presence of test antibodies at varying concentrations and effector (NK) cells at a 5:1 effector to target ratio for 4 h at 37°C. Target cells incubated with complete medium was used to determine spontaneous release, and target cells incubated with 0.5% Triton X 100 (Sigma Aldrich Corp.) was used to determine maximum release. 51Cr released in the culture supernatant was measured by a gamma counter (ISODATA). The cytotoxicity was expressed as the percentage of specific lysis and calculated as follows: 1 − [51Cr release of test samples − spontaneous 51Cr release/maximum 51Cr release − spontaneous 51Cr release] × 100.

Tumor studies.

All in vivo procedures were performed in accordance with Biogen Idec Institutional Animal Care and Use Committee guidelines. All mice (Charles River Laboratories, Inc.) were 8–12 weeks old at the time of cell implantation. MDA-MB-231 breast cancer cells (5 × 106 cells per mouse) or NCI-N87 gastric cancer cells (2 × 106 cells per mouse) in 200 µl media containing 50% Matrigel™ Matrix (BD Biosciences) were implanted subcutaneously into the right flank of female CB17 SCID mice (CB17-Prkdcscid/NCrCrl). WiDr colon carcinoma cells (5 × 106 cells per mouse) in 200 µl media were inoculated into the female nu/nu mice (Crl:NU-Foxn1nu). Tumors were size-matched across groups prior to initiation of treatment.

For the adenoviral delivery study, a single intratumoral injection of adeno-TWEAK,9 or the control adeno-GFP, (1 × 1011 viral particles) was administered to the mice (n = 7 mice per group) at day 9, when tumors had reached approximately 90 mm3. At the conclusion of the study (day 76 post-implantation), the tumors were weighed and then formalin-fixed and embedded in paraffin for histological analysis. For the Fc-TWEAK study, bi-weekly intratumoral injections of 100 µg of human Fc-TWEAK12 or vehicle control (PBS) (n = 8 mice per group) was begun at day 19 post-implantation. At the conclusion of the study (day 99 post-implantation), the tumors were weighed.

All antibodies (BIIB036; control Ig; hIgG1agly and hIgG4Pagly versions of BIIB036) were administered via intraperitoneal injection, once a week for 6 weeks. When average tumor volume reached ~220 mm3, mice were assigned to groups (n = 10 per group for WiDr and NCI-N87, n = 9 per group for MDA-MB-231) and antibody treatments were initiated.

During the course of the xenograft studies, tumors were measured twice a week using digital calipers and body weights were recorded. Tumor volume was calculated using the formula: L × W2/2 = mm3, where L is the length and W the tumor width. One way repeated measures ANOVA followed by Dunett's post test was used to evaluate differences between test and control groups across the study. Student's t-test (two-tail) was used to determine statistical significance of differences in mean tumor volume of test groups compared to control groups on individual measurement days.

Histological analysis.

Paraffin embedded tumor sections were alternatively stained with hematoxylin and eosin (H&E); anti-Ki-67 antibody to detect proliferating cells (mouse anti-human Ki-67); or stained with the TUNEL assay (ApopTag Peroxidase In Situ Apoptosis Detection Kit, Intergen Co.) to detect apoptotic cells.

Tumor sections (3 µm) were stained with a rabbit anti-cleaved Caspase 3 monoclonal antibody (Cell Signaling, Danvers, MA) or control rabbit IgG (Vector Lab) on a Ventana Discovery XT Instrument. The amount of cleaved Caspase 3 staining was quantified using Aperio's Spectrum Software Image Analysis algorithm for nuclear stain.

For the metastasis study, at terminal sacrifice (40 days post final dosing of antibody), axillary lymph nodes of mice were examined grossly for the presence of metastatic lesions and the volume of the lymph nodes was measured using calipers. Nodes from a representative set of mice in each group were harvested and placed in 10% neutral buffered formalin. The samples were processed and stained with anti-pan cytokeratin (Ventana) for histopathologic evaluation to determine presence of epithelial cells, i.e., tumor micro-metastases. The lymph nodes were examined for cytokeratin staining at 100x magnification. The lymph nodes were measured in situ and the volume calculated using the same formula as for tumor volume, L × W2/2 = mm3. Left and right axillary lymph node volumes of each animal were combined. The mean extent of micrometastases was scored as the percentage of the lymph node that stained positive with cytokeratin. Lymph nodes were scored blindly. The mean percentages were calculated for each group, and the p value was calculated by Wilcoxon signed rank test.


The authors acknowledge Bob Dunstan for caspase-3 and cytokeratin staining and scoring, Dian Olson for contribution to cell viability assays, and Michael Corbley for his many insights.

Conflict of Interest

The authors acknowledge a conflict of interest as employees and stock holders of Biogen Idec.

Supplementary Material

Supplementary Material:


1. Daniel D, Wilson NS. Tumor necrosis factor: renaissance as a cancer therapeutic? Curr Cancer Drug Targets. 2008;8:124–131. [PubMed]
2. Buchsbaum DJ, Zhou T, Lobuglio AF. TRAIL receptor-targeted therapy. Future Onc. 2006;2:493–508. [PubMed]
3. Wiezorek J, Holland P, Graves J. Death receptor agonists as a targeted therapy for cancer. Clin Cancer Res. 2010;16:1701–1708. [PubMed]
4. Burkly LC, Michaelson JS, Hahm K, Jakubowski A, Zheng TS. TWEAKing tissue remodeling by a multifunctional cytokine: role of TWEAK/Fn14 pathway in health and disease. Cytokine. 2007;40:1–16. [PubMed]
5. Chicheportiche Y, Bourdon PR, Xu H, Hsu YM, Scott H, Hession C, et al. TWEAK, A new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. J Biol Chem. 1997;272:32401–32410. [PubMed]
6. Wiley SR, Winkles JA. TWEAK, a member of the TNF superfamily, is a multifunctional cytokine that binds the TweakR/Fn14 receptor. Cytokine Growth Factor Rev. 2003;14:241–249. [PubMed]
7. Feng SL, Guo Y, Factor VM, Thorgeirsson SS, Bell DW, Testa JR, et al. The Fn14 immediate-early response gene is induced during liver regeneration and highly expressed in both human and murine hepatocellular carcinomas. A J Pathol. 2000;156:1253–1261. [PubMed]
8. Girgenrath M, Weng S, Kostek CA, Browning B, Wang M, Brown SA, et al. TWEAK, via its receptor Fn14, is a novel regulator of mesenchymal progenitor cells and skeletal muscle regeneration. EMBO J. 2006;25:5826–5839. [PubMed]
9. Jakubowski A, Ambrose C, Parr M, Lincecum JM, Wang MZ, Zheng TS, et al. TWEAK induces liver progenitor cell proliferation. J Clin Invest. 2005;115:2330–2340. [PMC free article] [PubMed]
10. Wiley SR, Cassiano L, Lofton T, Davis-Smith T, Winkles JA, Lindner V, et al. A novel TNF receptor family member binds TWEAK and is implicated in angiogenesis. Immunity. 2001;15:837–846. [PubMed]
11. Han H, Bearss DJ, Browne LW, Calaluce R, Nagle RB, Von Hoff DD. Identification of differentially expressed genes in pancreatic cancer cells using cDNA microarray.[ erratum appears in Cancer Res 2002; 62:4532] Cancer Res. 2002;62:2890–2896. [PubMed]
12. Michaelson JS, Cho S, Browning B, Zheng TS, Lincecum JM, Wang MZ, et al. Tweak induces mammary epithelial branching morphogenesis. Oncogene. 2005;24:2613–2624. [PubMed]
13. Tran NL, McDonough WS, Donohue PJ, Winkles JA, Berens TJ, Ross KR, et al. The human Fn14 receptor gene is upregulated in migrating glioma cells in vitro and overexpressed in advanced glial tumors. A J Pathol. 2003;162:1313–1321. [PubMed]
14. Tran NL, McDonough WS, Savitch BA, Fortin SP, Winkles JA, Symons M, et al. Increased fibroblast growth factor-inducible 14 expression levels promote glioma cell invasion via Rac1 and nuclear factor{kappa} B and correlate with poor patient outcome. Cancer Res. 2006;66:9535–9542. [PubMed]
15. Wang S, Zhan M, Yin J, Abraham JM, Mori Y, Sato F, Xu Y, et al. Transcriptional profiling suggests that Barrett's metaplasia is an early intermediate stage in esophageal adenocarcinogenesis. Oncogene. 2006;25:3346–3356. [PubMed]
16. Culp PA, Choi D, Zhang Y, Yin J, Seto P, Ybarra SE, et al. Antibodies to TWEAK receptor inhibit human tumor growth through dual mechanisms. Clin Cancer Res. 2010;16:497–508. [PubMed]
17. Willis AL, Tran NL, Chatigny JM, Charlton N, Vu H, Brown SA, et al. The fibroblast growth factor-inducible 14 receptor is highly expressed in HER2-positive breast tumors and regulates breast cancer cell invasive capacity. Mol Cancer Res. 2008;6:725–734. [PMC free article] [PubMed]
18. Brown SA, Richards CM, Hanscom HN, Feng SL, Winkles JA. The Fn14 cytoplasmic tail binds tumournecrosis-factor-receptor-associated factors 1, 2, 3 and 5 and mediates nuclear factorkappaB activation. Biochem J. 2003;371:395–403. [PubMed]
19. Saitoh T, Nakayama M, Nakano H, Yagita H, Yamamoto N, Yamaoka S. TWEAK induces NFκB2 p100 processing and long lasting NFκB activation. J Biol Chem. 2003;278:36005–36012. [PubMed]
20. Bover LC, Cardo-Vila M, Kuniyasu A, Sun J, Rangel R, Takeya M, et al. A previously unrecognized protein-protein interaction between TWEAK and CD163: potential biological implications. J Immunol. 2007;178:8183–8194. [PubMed]
21. Nakayama M, Harada N, Okumura K, Yagita H. Characterization of murine TWEAK and its receptor (Fn14) by monoclonal antibodies. Biochem Biophys Res Comm. 2003;306:819–825. [PubMed]
22. Nakayama M, Ishidoh K, Kayagaki N, Kojima Y, Yamaguchi N, Nakano H, et al. Multiple pathways of TWEAK-induced cell death. J Immunol. 2002;168:734–743. [PubMed]
23. Schneider P, Schwenzer R, Haas E, Muhlenbeck F, Schubert G, Scheurich P. TWEAK can induce cell death via endogenous TNF and TNF receptor 1. Eur J Immunol. 1999;29:1785–1792. [PubMed]
24. Vince JE, Chau D, Callus B, Wong WW, Hawkins CJ, Schneider P, et al. TWEAK-FN14 signaling induces lysosomal degradation of a cIAP1-TRAF2 complex to sensitize tumor cells to TNFalpha. J Cell Biol. 2008;182:171–184. [PMC free article] [PubMed]
25. Wilson CA, Browning JL. Death of HT29 adenocarcinoma cells induced by TNF family receptor activation is caspase-independent and displays features of both apoptosis and necrosis. Cell Death Diff. 2002;9:1321–1333. [PubMed]
26. Angal S, King DJ, Bodmer MW, Turner A, Lawson AD, Roberts G, et al. A single amino acid substitution abolishes the heterogeneity of chimeric mouse/human (IgG4) antibody. Mol Immunol. 1993;30:105–108. [PubMed]
27. Dong J, Demarest SJ, Sereno A, Tamraz S, Langley E, Doern A, et al. Combination of two insulin-like growth factor-I receptor inhibitory antibodies targeting distinct epitopes leads to an enhanced antitumor response. Mol Cancer Ther. 2010;9:2593–2604. [PubMed]
28. Holler N, Tardivel A, Kovacsovics-Bankowski M, Hertig S, Gaide O, Martinon F, et al. Two adjacent trimeric Fas ligands are required for Fas signaling and formation of a death-inducing signaling complex. Mol Cell Biol. 2003;23:1428–1440. [PMC free article] [PubMed]
29. Co MS, Avdalovic NM, Caron PC, Avdalovic MV, Scheinberg DA, Queen C. Chimeric and humanized antibodies with specificity for the CD33 antigen. J Immunol. 1992;148:1149–1154. [PubMed]
30. Wicovsky A, Salzmann S, Roos C, Ehrenschwender M, Rosenthal T, Siegmund D, et al. TNF-like weak inducer of apoptosis inhibits proinflammatory TNF receptor-1 signaling. Cell Death Differ. 2009;16:1445–1459. [PubMed]
31. Sarmay G, Lund J, Rozsnyay Z, Gergely J, Jefferis R. Mapping and comparison of the interaction sites on the Fc region of IgG responsible for triggering antibody dependent cellular cytotoxicity (ADCC) through different types of human Fc gamma receptor. Mol Immunol. 1992;29:633–639. [PubMed]
32. Nakayama M, Ishidoh K, Kojima Y, Harada N, Kominami E, Okumura K, et al. Fibroblast growth factor-inducible 14 mediates multiple pathways of TWEAK-induced cell death. J Immunol. 2003;170:341–348. [PubMed]
33. Dogra C, Changotra H, Wedhas N, Qin X, Wergedal JE, Kumar A. TNF-related weak inducer of apoptosis (TWEAK) is a potent skeletal muscle-wasting cytokine. FASEB J. 2007;21:1857–1869. [PubMed]
34. Chicheportiche Y, Chicheportiche R, Sizing I, Thompson J, Benjamin CB, Ambrose C, et al. Proinflammatory activity of TWEAK on human dermal fibroblasts and synoviocytes: blocking and enhancing effects of anti-TWEAK monoclonal antibodies. Arthritis Res. 2002;4:126–133. [PMC free article] [PubMed]
35. Zimmermann M, Koreck A, Meyer N, Basinski T, Meiler F, Simone B, et al. TNF-like weak inducer of apoptosis (TWEAK) and TNFalpha cooperate in the induction of keratinocyte apoptosis. J Allergy Clin Immunol. 2011;127:200–207. [PubMed]
36. Wilson NS, Yang B, Yang A, Loeser S, Marsters S, Lawrence D, et al. An Fcgamma receptor-dependent mechanism drives antibody-mediated target-receptor signaling in cancer cells. Cancer Cell. 2011;19:101–113. [PubMed]
37. Watts GS, Tran NL, Berens ME, Bhattacharyya AK, Nelson MA, Montgomery EA, et al. Identification of Fn14/TWEAK receptor as a potential therapeutic target in esophageal adenocarcinoma. Int J Cancer. 2007;121:2132–2139. [PubMed]
38. Michaelson JS, Burkly LC. Therapeutic targeting of TWEAK/Fnl4 in cancer: exploiting the intrinsic tumor cell killing capacity of the pathway. Results Probl Cell Differ. 2009;49:145–160. [PubMed]
39. Lynch CN, Wang YC, Lund JK, Chen YW, Leal JA, Wiley SR. TWEAK induces angiogenesis and proliferation of endothelial cells. J Biol Chem. 1999;274:8455–8459. [PubMed]
40. Donohue PJ, Richards CM, Brown SA, Hanscom HN, Buschman J, Thangada S, et al. TWEAK is an endothelial cell growth and chemotactic factor that also potentiates FGF-2 and VEGF-A mitogenic activity. Arterioscler Thromb Vasc Biol. 2003;23:594–600. [PubMed]
41. Ho DH, Vu H, Brown SA, Donohue PJ, Hanscom HN, Winkles JA. Soluble tumor necrosis factor-like weak inducer of apoptosis overexpression in HEK293 cells promotes tumor growth and angiogenesis in athymic nude mice. Cancer Res. 2004;64:8968–8972. [PubMed]
42. Kawakita T, Shiraki K, Yamanaka Y, Yamaguchi Y, Saitou Y, Enokimura N, et al. Functional expression of TWEAK in human colonic adenocarcinoma cells. Int J Oncol. 2005;26:87–93. [PubMed]

Articles from mAbs are provided here courtesy of Taylor & Francis