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Nucleic Acids Res. 1984 January 11; 12(1 Pt 2): 717–729.
PMCID: PMC321087

Plasmid mapping computer program.


Three new computer algorithms are described which rapidly order the restriction fragments of a plasmid DNA which has been cleaved with two restriction endonucleases in single and double digestions. Two of the algorithms are contained within a single computer program (called MPCIRC). The Rule-Oriented algorithm, constructs all logical circular map solutions within sixty seconds (14 double-digestion fragments) when used in conjunction with the Permutation method. The program is written in Apple Pascal and runs on an Apple II Plus Microcomputer with 64K of memory. A third algorithm is described which rapidly maps double digests and uses the above two algorithms as adducts. Modifications of the algorithms for linear mapping are also presented.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Parker RC, Watson RM, Vinograd J. Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis. Proc Natl Acad Sci U S A. 1977 Mar;74(3):851–855. [PubMed]
  • Schroeder JL, Blattner FR. Least-squares method for restriction mapping. Gene. 1978 Oct;4(2):167–174. [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press