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Nucleic Acids Res. 1984 January 11; 12(1 Pt 2): 695–702.
PMCID: PMC321085

Molecular weight determination program.


A computer program is described that will determine the molecular weight of DNA, RNA or protein molecules separated according to size by gel electrophoresis. It uses the sizes and migration distances of known molecules in a reference lane to compute a second or third order equation whose curve best fits the data points. It then computes the sizes of all molecules from this equation. Migration distances are measured and entered using an analog tablet. The program is written in Apple Pascal and designed to run on an Apple II Plus computer.

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Selected References

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  • Pettersson U, Mulder C, Deluis H, Sharp PA. Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI. Proc Natl Acad Sci U S A. 1973 Jan;70(1):200–204. [PubMed]
  • Maniatis T, Jeffrey A, van deSande H. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975 Aug 26;14(17):3787–3794. [PubMed]
  • Sharp PA, Sugden B, Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. [PubMed]
  • Duggleby RG, Kinns H, Rood JI. A computer program for determining the size of DNA restriction fragments. Anal Biochem. 1981 Jan 1;110(1):49–55. [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press