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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Nat Struct Mol Biol. Author manuscript; available in PMC Apr 30, 2012.
Published in final edited form as:
PMCID: PMC3210390
Tfam, a mitochondrial transcription and packaging factor, imposes a U-turn on mitochondrial DNA
Huu B. Ngo,1 Jens T. Kaiser,2 and David C. Chan1,3
1Division of Biology, California Institute of Technology, Pasadena, CA, USA.
2Division of Chemistry, California Institute of Technology, Pasadena, CA, USA.
3Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA, USA.
Correspondence should be addressed to D.C.C. (dchan/at/
Tfam, a DNA binding protein with tandem HMG (high mobility group)-box domains, plays a central role in expression, maintenance, and organization of the mitochondrial genome. It activates transcription from mitochondrial promoters and organizes the mitochondrial genome into nucleoids. Using X-ray crystallography, we show that human Tfam forces promoter DNA to undergo a U-turn, reversing the direction of the DNA helix. Each HMG-box domain wedges into the DNA minor groove to generate two kinks on one face of the DNA. On the opposite face, a positively charged α-helix serves as a platform to facilitate DNA bending. The structural principles underlying DNA bending converge with those of the unrelated HU family proteins, which play analogous architectural roles in organizing bacterial nucleoids. The functional importance of this extreme DNA bending is promoter-specific and appears related to the orientation of Tfam on the promoters.
Keywords: Tfam, HMG-box, transcriptional activation, mtDNA, nucleoid
Among eukaryotic protein complexes, the mitochondrial oxidative phosphorylation (OXPHOS) machinery is unique in having a bigenomic origin. Most of the OXPHOS machinery is encoded by the nuclear genome, but 13 essential subunits of respiratory chain complexes I, III, IV, and V are encoded by the 16 kb mitochondrial genome. Tfam (transcription factor A, mitochondrial; also known as mtTFA), a DNA binding protein in mitochondria, is a central player in expression and maintenance of mitochondrial DNA (mtDNA), and therefore is essential for ATP production via OXPHOS1, 2. The mammalian mitochondrial genome contains three promoters--the light strand promoter (LSP), the heavy strand promoter 1 (HSP1), and the heavy strand promoter 2 (HSP2)--that drive expression of mtDNA transcripts. Transcription from LSP and HSP1 has been reconstituted in vitro, and normal levels of transcription require Tfam14. Moreover, because truncated RNA transcripts from LSP are used to prime DNA replication, Tfam is secondarily essential for mtDNA replication. Mice lacking Tfam therefore show impaired mtDNA transcription and loss of mtDNA, leading to bioenergetic insufficiency and embryonic lethality5.
Upstream of both the LSP and HSP1 transcriptional start sites, Tfam recognizes a binding site that has been defined by DNAse I footprinting experiments3, 4. Tfam contains two HMG-box domains followed by a short C-terminal tail6. HMG-box domains are DNA binding motifs that bind to the minor groove of DNA and, in some cases, result in DNA bending7. Tfam belongs to the HMG-box subgroup that contains tandem HMG-box domains7. Several proteins in this subgroup, like Tfam, play important structural roles in DNA organization, but there is currently no information about how two HMG-box domains can be spatially coordinated to affect DNA structure. The C-terminal tail of Tfam is essential for transcriptional activation8 and also for its physical association with Tfb2m9, another transcription factor required for mtDNA transcription. As a result, it has been proposed that Tfam binding allows recruitment of Tfb2m via the C-terminal tail.
In addition to its transcriptional function, Tfam is thought to play a major role in mtDNA packaging10, 11. Although Tfam functions as a sequence-specific transcription factor, it also has high affinity for non-specific DNA. Unlike nuclear DNA, mtDNA is not associated with histones. mtDNA genomes within the mitochondrial matrix are organized into compact DNA–protein complexes termed nucleoids12. Tfam is one of the most abundant proteins associated with mtDNA nucleoids13 and its levels have been estimated to be sufficient to coat the entire mitochondrial genome14. The levels of Tfam correlate with the levels of mtDNA15. The yeast ortholog of Tfam, Abf2, has no role in transcription and its major function is thought to be in organization of the mitochondrial genome16.
To understand how Tfam mediates these multiple functions on mitochondrial DNA, we have solved the structure of human Tfam in complex with the LSP binding site. The structure shows how Tfam coordinates its two HMG-box domains to impose a dramatic U-turn on the DNA. To bend DNA, Tfam utilizes structural principles similar to that found in the HU family of prokaryotic nucleoid proteins, which, like Tfam, play architectural roles in genome organization. Moreover, we find this DNA bending is more important for transcriptional activation at LSP than HSP1.
Structure determination
We solved the 2.5 Å crystal structure of human Tfam bound to a 28 bp DNA fragment derived from LSP (Table 1 and Fig. 1a–d). The recombinant Tfam used (residues 43–246) represents the full-length, mature Tfam after cleavage of the N-terminal mitochondrial leader sequence6. The DNA fragment includes a ~22 bp sequence identified as a high-affinity Tfam binding site by DNAse footprinting and has 2 half-sites that interact with the HMG-box domains3. A selenomethionine-substituted Tfam–mtDNA complex was used for structure determination at 2.5 Å by multi-wavelength anomalous diffraction (MAD) analysis. The crystallographic statistics of data collection and refinement are presented in Table 1. The electron density map was of sufficient quality to build almost all of the protein (residues 43–237) and all 28 base pairs of mtDNA. Model building and refinement produced a final structure with excellent stereochemistry, with an Rfree of 24.7% and an Rwork of 19.8%.
Table 1
Table 1
Data collection, phasing and refinement statistics
Figure 1
Figure 1
Overview of the Tfam–mtDNA complex
This crystal structure is the first one of a native tandem HMG-box protein in complex with DNA. In a previous study17, the NMR structure was solved of a chimeric molecule consisting of the HMG-box domain of SRY (a single HMG-box protein) fused to one of the two HMG-box domains of HMGB1 (a tandem HMG-box protein). This artificial molecule is nonphysiological, and its structure in complex with DNA does not resemble the structure described here.
Tfam imposes a severe bend on LSP mtDNA
The most striking feature of the structure is that binding of a Tfam monomer dramatically distorts the DNA into a U-shape, causing a reversal in the direction of the DNA helical axis (Fig. 1c, d). Each HMG-box folds into a 3-helix motif whose concave surface intercalates between the bases in the minor groove of an LSP half-site (Fig. 1c). These two intercalations result in two sharp kinks on one face of the DNA helix. The buried contact area of the first HMG-box domain (box A) with DNA is 1566 Å2, and the corresponding surface area of the second HMG-box (box B) is nearly as extensive at 1404 Å2 (Fig. 1e, f). The linker connecting the two HMG-box domains forms an α-helix around which the DNA wraps (contact area 864 Å2) (Fig. 1c, d, and f). As described in detail later, basic side chains in the linker interact with the negatively charged phosphates in the bent DNA backbone. The C-terminal tail also contacts DNA (580 Å2) and the first part of this region extends the third helix of the second HMG-box domain. Therefore, all four regions (Fig. 1a) of Tfam--the two HMG-box domains, the linker, and C-terminal tail--make extensive contact with the DNA.
The structure agrees well with previous DNAse I footprinting and methylation interference experiments probing the binding of Tfam to LSP DNA3, 18. The Tfam monomer accounts for the large recognition site identified by a combination of DNAse I footprinting and sequence analysis3, 4, 8. Each HMG-box domain binds to one of the two half-sites identified by sequence analysis8. In previous methylation interference experiments18, the methylation by dimethylsulfate (DMS) of selected adenines was associated with reduced binding of Tfam. DMS methylates adenine at the N3 atom, which is located within the minor groove and would sterically block subsequent Tfam binding. In our crystal structure, all the adenines identified by Clayton and colleagues18 reside in a position where Tfam contacts the DNA minor groove and causes widening (Supplementary Fig. 1). In contrast, methylation of adenines located outside the contact area did not affect Tfam binding.
Our crystal structure indicates that Tfam binds mtDNA as a monomer. Human Tfam without DNA is monomeric, but it has been suggested that Tfam assembles into dimers upon DNA binding19. The latter conclusion is tenuous, because it was based on a gel mobility assay that used extremely high concentrations of Tfam and DNA and does not give a definitive assessment of stoichiometry. To independently test the 1:1 stoichiometry found in our crystal structure, we analyzed Tfam and the Tfam–mtDNA complex in solution by size exclusion chromatography with in-line multi-angle light scattering analysis (SEC-MALS). The measured molar masses indicated that Tfam in isolation is monomeric and when complexed with DNA, forms a 1:1 complex (Fig. 2).
Figure 2
Figure 2
Molecular mass of the Tfam–mtDNA complex determined by SEC-MALS
Protein-DNA interactions
As in other HMG-box structures, each Tfam HMG-box domain folds into an L-shape composed of 3 α-helices, with the third helix forming the long axis (Fig. 1c). A hydrophobic core composed of Tyr57, Phe60, Trp88, and Tyr99 stabilizes the L-shaped configuration of the first HMG-box (Fig. 3a; Supplementary Fig. 2). Similarly, buried residues Tyr162, Tyr165, Trp189, and Tyr200 stabilize the second HMG-box domain (Fig. 3b; Supplementary Fig. 2). The overall fold of both HMG-box domains superimpose well with other HMG-boxes whose structures in complex with DNA have been solved (Fig. 3c).
Figure 3
Figure 3
Interactions of Tfam with DNA
In the Tfam–mtDNA complex, most of the side-chain–DNA contacts are not sequence-specific and occur on the sugar-phosphate backbone of the DNA. However, a small number of contacts to bases within the minor groove can be seen. HMG-box domains generally contain 1 or 2 hydrophobic residues that intercalate into the minor groove (highlighted in Supplementary Fig. 3). Consistent with this generalization, the HMG-box A of Tfam contains the first of these intercalating residues at position 58 (Leu58), which interacts with A8 (strand B) (Fig. 3d, red residue). A previous crystal structure of the isolated, HMG-box B of Tfam raised the issue of whether it was a non-canonical HMG-box domain with unusual binding properties19, because it appeared to lack both intercalating hydrophobic residues. Our Tfam–mtDNA structure clarifies this issue by showing that HMG-box B does contain DNA binding residues at these same positions, even though the residues are not nonpolar. In the first position, HMG-box B contains Asn163, which reaches into the minor groove and contacts the underlying thymine (T7, strand A). In the second position, Pro178 similarly inserts into the minor groove and contacts a guanine (G9, strand A) (Fig. 3e, red residues). In comparison to the previous structure of HMG-box B without DNA19, Pro178 has shifted >2 Å to make this contact with the DNA base.
Besides the interactions indicated above, several other contacts to DNA bases are apparent. In HMG-box A, contacts are observed between Ile81 and T19 (strand A), Tyr57 and G20 (strand A), and Ser61 and G20 (strand A) (Fig. 3d; Supplementary Fig. 2c). In addition, Ser61 and Ser55 indirectly interact with C9 (strand B) and T21 (strand A), respectively, via water molecules. In the HMG-box B (Fig. 3e; Supplementary Fig. 2d), contacts are observed between Arg157 and T24 (strand B), and Gln179 and C19 (strand B). The linker does not directly interact with DNA bases. However, it makes substantial contacts with DNA via charged or polar interactions (Fig. 3f, g). Lys147 contacts G16 (strand A). His137 and Arg140 both make contacts to the phosphate backbone. Other lysine residues in the linker region (Lys136, Lys139, and Lys146) make longer-range contacts (>3.35Å) with the sugar-phosphate backbone.
Similarity to HU and IHF nucleoid proteins
The conformations of the two half-sites bound by Tfam deviate substantially from canonical B-DNA (Fig. 4a–d). At each location, intercalation by the HMG-box results in substantial widening of the minor groove (Fig. 4a). There is local DNA unwinding, as indicated by sharp minima in the base twist (base step parameter) at the sites of intercalation (Fig. 4c). Globally, however, the DNA is not underwound, with an average helical twist of ~36°. The roll angle profile (Fig. 4b) shows two sharp peaks, which reflect distortions of base stacking due to acute DNA bending.
Figure 4
Figure 4
Comparison of Tfam and Hbb structures
The mode of DNA bending in the Tfam–mtDNA structure shows remarkable parallels with the HU protein family, which consists of DNA minor groove binding proteins that play architecture roles in prokaryotic DNA nucleoids20, 21. Integration host factor (IHF), HU, and Hbb are HU family proteins that contort their bound DNA into a U-shape20, 21. These proteins form dimers in which each subunit uses a "β-ribbon arm" to intercalate into the DNA minor groove (Fig. 4a, e). The dimerization interface between the two subunits is rich in positive residues and serves to neutralize the negative charges of the bent DNA backbone. The DNA fragments in the Tfam and Hbb complexes show similar profiles in the minor groove width, with two broad peaks corresponding to minor groove intercalations (Fig. 4a). The roll angles also show two peaks that signify the sharp bending of DNA. The peaks are slightly closer together in the Hbb (~9 bps apart) versus the Tfam structure (Fig. 4b). Superimposition of the DNA fragments reveals the similarity in overall geometry (Fig. 4f).
Both HMG-boxes and the linker are critical for DNA bending
To monitor DNA bending by Tfam, we developed a FRET (fluorescence resonance energy transfer)-based assay. The crystal structure shows that after binding of Tfam, the ends of the 28 bp LSP DNA fragment are brought to within 55 Å (measuring from the 5′ phosphate of one strand to the 5′ phosphate of the other strand), compared to the 95 Å separation found in a rod-like DNA fragment of identical length. To construct the FRET sensor, Cy3 (donor) and Cy5 (acceptor) fluorophores were covalently attached to opposite ends of the LSP fragment. Addition of Tfam to the labeled, double-stranded DNA resulted in a dose-dependent increase in acceptor emission and a decrease in donor emission (Table 2; Supplementary Fig. 4a, b). Control experiments confirmed that the acceptor emission depended on the presence of both the donor fluorophore and Tfam. The maximum FRET efficiency measured with wild-type Tfam corresponds to a calculated fluorophore separation of 59 Å, in good agreement with the crystal structure. The ability of Tfam to bend DNA is not restricted to the LSP template. Tfam was able to bend a DNA template lacking promoter sequences, and also one corresponding to HSP1 (Supplementary Fig. 4c, d).
Table 2
Table 2
DNA bending and binding of Tfam variants
Analysis of a panel of Tfam mutants indicated that coordinated binding of both HMG-box domains is important for effective bending of DNA (Table 2). HMG-box A alone bound to LSP DNA with the same affinity as full-length Tfam, consistent with previous studies19, 22. However, it showed a large reduction in DNA bending. HMG-box B alone showed much weaker affinity for LSP DNA (KD ~400 nM) and also showed a large reduction in DNA bending. In addition, we tested Tfam mutants with single point mutations in HMG-box residues that contact DNA (Table 2; Supplementary Fig. 4b). T77A, containing a mutation in HMG-box A, and Y162A, containing a mutation in HMG-box B, showed moderate reductions in DNA bending. Each of these residues makes contacts with the DNA backbone (Fig. 3d, e). Finally, we tested the effect of mutations in the positively charged residues in the linker helix. Single point mutations had little effect (data not shown). We therefore made a mutant, L6, in which 6 positively charged residues in the linker region were replaced by alanine. The L6 mutant showed >30% reduction in FRET, indicating that the linker region is important for DNA bending. All these mutants were well-folded, based on secondary structure analysis by circular dichroism (Supplementary Fig. 4e). In addition, the T77A, Y162A, and L6 mutants retained high affinity for DNA, as indicated by an assay monitoring the quenching of intrinsic tryptophan fluorescence upon DNA binding (Table 2; Supplementary Fig. 5).
Tfam bending mutants show promoter-specific defects
With mutants that reduce DNA bending by Tfam, we used in vitro transcription reactions containing mitochondrial RNA polymerase (Polrmt) and Tfb2m to test whether DNA bending is important for its transcriptional activation function (Fig. 5). Neither HMG-box A nor HMG-box B alone was able to activate transcription from LSP or HSP templates. In addition, Tfam containing both HMG-boxes but lacking the C-terminal tail was unable to activate transcription (Fig. 5a, b). These results are expected, because previous studies indicated that the C-terminal tail of Tfam is essential for transcriptional activation8. Interestingly, we found that both the T77A and the Y162A mutants were less efficient in promoting transcription from the LSP template. Y162A, which has a more severe bending defect, was more affected. Finally, the mutant L6, which has the strongest bending defect, showed a severe defect in transcriptional activation. The transcriptional defects were similar whether full-length or truncated LSP transcripts were quantified (Fig. 5c, d).
Figure 5
Figure 5
Tfam mutants with a selective defect at LSP
To test whether these Tfam mutants were generally defective in transcriptional activation, we examined their activity with an HSP1 template (Fig. 5b, e). In DNA bending measurements, these mutants showed defects in bending the HSP1 DNA template (Supplementary Fig. 4d), as was found previously with the LSP template. In transcriptional activation assays, however, the Y77A, Y162A, and L6 mutants were all efficient at stimulating transcripts from HSP1 (Fig. 5e). Quantification showed that all three mutants showed a similar transcriptional activation profile compared to wildtype Tfam.
Based on previous structural studies, a single HMG-box domain can bind to the DNA minor groove and sometimes cause bending of the DNA double helix. For example, the prototypical HMG-box protein Sry, which contains a single HMG-box domain, bends DNA ~70–80° upon binding to the minor groove23. This mode of DNA bending (Fig. 6a) superficially resembles that of the TATA binding protein Tbp, where binding of a β-sheet to the DNA minor groove again induces moderate bending towards the opposite direction24, 25.
Figure 6
Figure 6
Models for DNA bending and transcriptional activation
In comparison to these structures, the Tfam–mtDNA complex illustrates how spatial coordination of tandem HMG-box domains can be harnessed to impose even more extreme distortion onto DNA (Fig. 6b). Tfam belongs the subset of HMG-box proteins that contain tandem HMG-box domains. These HMG-box proteins generally show broad DNA binding and play important roles in regulating chromatin structure and function7. For example, Hmgb1 is an architectural protein on chromatin that has been implicated in transcription, chromatin organization, and genome stability26. In Tfam, the α-helical linker plays a key role by spatially coordinating the two HMG-box domains, so that they bind the DNA minor groove at sites located approximately one helical turn apart. Moreover, the linker further facilitates DNA bending by neutralizing the negative charges on the DNA backbone. Intriguingly, all the other dual HMG-box proteins in the human genome contain a cluster of 5–8 positively charged residues in the short region between the HMG-box domains (Supplementary Fig. 6). It will be interesting to determine whether these residues play a role analogous to that of the linker region in Tfam.
Although Tfam and the HU family of nucleoid proteins do not share sequence or structural homology, our studies indicate that they use remarkably analogous strategies to impose extreme bending onto DNA (Fig. 6b). The similarities between the Tfam–DNA and HU-family–DNA structures are intriguing, given that both proteins are thought to control the architecture of DNA in nucleoids. The DNA in our structure is from LSP and therefore is more directly related to mitochondrial transcriptional activation. However, the structure is likely to also be relevant for the role of Tfam in nucleoid organization, given the ability of Tfam to bend generic DNA (Supplementary Fig. 4c).
Our results indicate that the relative importance of extreme DNA bending by Tfam depends on the mitochondrial promoter. Previous studies indicated that the C-terminal tail of Tfam is essential for transcriptional activation8 and physical interaction with Tfb2m9. In the crystal structure, when Tfam is bound to the LSP promoter, the HMG-box B domain binds at the half-site further upstream from the transcription start site (Fig. 1c, ,6c).6c). Without DNA bending, the C-terminal tail would face away from the transcriptional start site (Fig. 6d). However, the DNA U-turn redirects the C-terminal tail towards the transcriptional machinery (Fig. 6c). We speculate that one of the functions of DNA bending by Tfam is to enable the C-terminal tail to interact with the rest of transcriptional machinery. Based on previous results9, Tfb2m is a favored candidate for such an interaction. Remarkably, transcription from HSP1 is much less sensitive to DNA bending by Tfam. Based on sequence analysis, the Tfam binding sites in HSP1 versus LSP are in reverse orientations relative to the direction of transcription (Fig. 1b)4, 8. When Tfam is bound to the HSP1 promoter, HMG-box B would be expected to bind the half site adjacent to the transcriptional start. The C-terminal tail is therefore in proximity to the transcriptional machinery, regardless of whether the DNA is bent or not. In future studies, it will be important to test this proposal by determining the structure of Tfam in complex with HSP1 promoter DNA.
Tfam purification
The human TFAM gene was cloned into the pET28a expression vector (Novagen) between the BamHI and XhoI sites. This construct encodes residues 43–246, corresponding to full-length Tfam after cleavage of the N-terminal mitochondrial leader sequence (residues 1–42). Tfam mutants were constructed by PCR using oligonucleotides encoding mutations. Plasmids were transformed into BL21 (DE3) E. coli (Invitrogen). LB broth (20 ml) containing 50 µg ml−1 kanamycin was inoculated with a single colony and grown overnight at 37°C. The overnight culture was diluted to 4 liters and grown until an OD600 of 1.0. After induction with 1 mM isopropyl β-D-1-thiogalactopyranoside, the culture was grown overnight at room temperature. The cells were harvested and stored at −80 °C. Five grams of cells were resuspended in 50 ml lysis buffer (20 mM Tris-HCl, 500 mM NaCl, pH 7.5) and sonicated for 5 min (10 s on and 20 s off) on ice. After centrifugation at 4.3×104 g for 30 min at 4 °C, His-tagged Tfam was purified from the supernatant with 3 mls of Talon Cobalt resin (Clontech). The protein was eluted (20 mM Tris-HCl, 500 mM NaCl, 300 mM imidazole,pH 7.5) and further purified by gel filtration chromatography using a Hi-Load Superdex 200 16/60 column (GE Healthcare) pre-equilibrated with running buffer (20 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, pH 7.5) in an AKTA Purifier (Amersham). The peak fraction was collected and concentrated to 17–20 mg ml−1 using Amicon Ultra-15 concentrators (Millipore) with a molecular weight cutoff of 10 kDa. The protein was flash-frozen in liquid nitrogen and stored at −80 °C. Selenomethionine-substituted Tfam was produced by the metabolic inhibition method34, and preparative buffers contained 5 mM β-mercaptoethanol instead DTT. Proteins were analyzed by DNA binding and circular dichroism analysis, as detailed in the Supplementary Methods.
Crystallization, data collection, and structure determination
The duplex LSP fragment was made by annealing complementary oligonucleotides (5′-TGTTAGTTGGGGGGTGACTGTTAAAAGT-3′ and 5′-ACTTTTAACAGTCACCCCCCAACTAACA-3′) in buffer (10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mM EDTA) at a concentration of 0.9 mM. The mixture was incubated at 95°C for 5 min, 75°C for 5 min, and room temperature >5 h.
To form Tfam–mtDNA complexes, Tfam was mixed with duplex DNA in a 1.3:1 molar ratio. The mixture was incubated at room temperature for 30 min and then on ice for 2 h. Crystallization trials by hanging drop-vapor diffusion at room temperature identified a condition [29% PEG 400 (w/v), 0.15 M calcium acetate, 0.1 M sodium acetate (pH 4.2), 400 mM NDSB211 (dimethyl-2(-hydroxyethyl)-(3-sulfopropyl)-ammonium)] that yielded rod-shaped crystals. Diffraction data were collected on frozen crystals on beamline 12-2 at the Stanford Synchrotron Radiation Lightsource. All data were processed with IMOSFLM35 or XDS36, and merged using SCALA37 as implemented in CCP438. A selenomethionine-substituted-Tfam–mtDNA complex was used for phasing. Using intensity data at 3.0 Å from three wavelengths, all five selenium sites were located by PHENIX39. After solvent flattening and density modification in PHENIX, the map revealed clear density for the protein and DNA. Manual model building in COOT40 using the 3.0 Å experimental map generated a starting model. Refinement of the best solutions was carried out using PHENIX, with an initial round of rigid body refinement followed by a round of simulated annealing. Refinement against a 2.5 Å data set produced an excellent map with density for most of the side chains. After a few rounds of model adjustment and refinement with TLS obtained from the TLSMD server41, the Rwork converged to 19.8% and the Rfree to 24.7%. The final model includes residues 43–237 of Tfam and all the nucleotides. The current model has excellent stereochemistry with no Ramachandran outliers, as assessed by MOLPROBITY42.
FRET experiments
To generate LSP, HSP, and non-promoter templates, the following complementary oligonucleotides were annealed as described above:
Additional details of the FRET measurements and analysis of the FRET data are described in the Supplementary Methods.
Size-exclusion chromatography with multi-angle light scattering (SEC-MALS)
SEC-MALS experiments were performed at room temperature by loading samples on a Shodex KW 803 column with a Dawn Heleos MALS detector (Wyatt Technology). The column was eluted with buffer containing 20 mM Tris-HCl (pH7.5), 300 mM NaCl, and 1 mM DTT. A dn/dc (refractive index increment) value of 0.185 mL mg−1 was used. Bovine serum albumin was used as an isotropic scatterer for detector normalization. The light scattered by a protein is directly proportional to its weight-average molecular mass and concentration.
in vitro transcription reactions
DNA fragments corresponding to LSP (positions 1–477) and HSP1 (positions 499–741) of human mtDNA were cloned into the pSP65 vector at the BamHI and SalI sites. After digestion with BamHI for LSP and SalI for HSP1, the linearized plasmids were used as templates in a transcriptional run-off assay. Transcription reactions were carried out as described43 with modifications. Template DNA (5 nM) was added to the reaction mix [10 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM DTT, 100 µg ml-1 BSA, and 40 units of RNaseOut (Invitrogen)] for 5 min, and then Tfam, Tfb2m (30 nM, Enzymax), and Polrmt (30 nM, Enzymax) were sequentially added, with a 1 min incubation between each addition. After addition of rNTPs [400 µM rATP, 150 µM rCTP, 150 µM rGTP, 15 µM rUTP (Promega), 0.2 µM (α-32P) rUTP (3,000 Ci mmol−1, Perkin Elmer)], the reaction was incubated for 3 h at 33°C, and stopped by addition of 25 µL of stop buffer (80% formamide (v/v), 10 mM EDTA, pH 8.0, 0.025% xylene cyanol (w/v), 0.025% bromophenol blue (w/v)). Samples were heated to 90 °C for 5 min and separated on 5% polyacrylamide gels (w/v) containing 8 M urea in 1×TBE. The gels were fixed in 7% acetic acid (v/v), dried, and exposed to a phosphorimager screen. The data were collected on a Storm 880 phosphorimager (Molecular Dynamics), and quantified using ImageQuant 5.2 Software.
Supplementary Material
We thank Nickie Chan (California Institute of Technology) for making some mutant constructs, Yan Zhang and Zhenfeng Liu (California Institute of Technology) for suggestions on phase determination and structure refinement, Troy Walton (California Institute of Technology) for advice on SEC-MALS, Shu-ou Shan (California Institute of Technology) for use of equipment and insightful discussions, the staff at SSRL for technical support with crystal screening and data collection, and members of the Chan lab for critical reading of the manuscript. We acknowledge the Gordon and Betty Moore Foundation for support of the Molecular Observatory at Caltech. SSRL is supported by the US DOE and NIH. This work was supported by NIH grant GM083121 (D.C.C.).
Supplementary Information
Supplemental Information includes Supplementary Fig. 1–6 and Supplementary Methods.
Accession codes. Protein Data Bank: Atomic coordinates and structure factors for the Tfam–mtDNA complex have been deposited under the accession code 3TMM.
Author Contributions
H.B.N. and D.C.C. designed the experiments, analyzed the data, and wrote the paper. H.B.N. carried out the crystallography and performed the experimental work. J.T.K. helped with the crystallographic analysis.
Competing financial interests
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