Distribution of vesicular monoamine transporter 2 protein in human brain: implications for brain imaging studies

doi:10.1038/jcbfm.2011.63

J Cereb Blood Flow Metab. 2011 October; 31(10): 2065–2075.

Published online 2011 April 27. doi: 10.1038/jcbfm.2011.63

PMCID: PMC3208151

Distribution of vesicular monoamine transporter 2 protein in human brain: implications for brain imaging studies

Junchao Tong,^1,²^* Isabelle Boileau,² Yoshiaki Furukawa,³ Li-Jan Chang,¹ Alan A Wilson,⁴ Sylvain Houle,⁴ and Stephen J Kish^1,²

¹Human Brain Laboratory, Centre for Addiction and Mental Health, Toronto, Ontario, Canada

²Addiction Imaging Research Group, Vivian M Rakoff PET Centre, Centre for Addiction and Mental Health, Toronto, Ontario, Canada

³Department of Neurology, Juntendo Tokyo Koto Geriatric Medical Center, Faculty of Medicine, University and Post Graduate University of Juntendo, Tokyo, Japan

⁴Vivian M Rakoff PET Centre, Centre for Addiction and Mental Health, Toronto, Ontario, Canada

^*Human Brain Laboratory, Centre for Addiction and Mental Health, 250 College Street, Toronto, ON, Canada, M5T 1R8. E-mail: junchao_tong/at/camh.net

Received December 9, 2010; Revised February 4, 2011; Accepted April 1, 2011.

Abstract

The choice of reference region in positron emission tomography (PET) human brain imaging of the vesicular monoamine transporter 2 (VMAT2), a marker of striatal dopamine innervation, has been arbitrary, with cerebellar, whole cerebral, frontal, or occipital cortices used. To establish whether levels of VMAT2 are in fact low in these cortical areas, we measured VMAT2 protein distribution by quantitative immunoblotting in autopsied normal human brain (n=6). Four or five species of VMAT2 immunoreactivity (75, 55, 52, 45, 35

kDa) were detected, which were all markedly reduced in intensity in nigrostriatal regions of patients with parkinsonian conditions versus matched controls (n=9 to 10 each). Using the intact VMAT2 immunoreactivity, cerebellar and cerebral neocortices had levels of the transporter >100-fold lower than the VMAT2-rich striatum and with no significant differences among the cortical regions. We conclude that human cerebellar and cerebral cortices contain negligible VMAT2 protein versus the striatum and, in this respect, all satisfy a criterion for a useful reference region for VMAT2 imaging. The slightly lower PET signal for VMAT2 binding in occipital (the currently preferred reference region) versus cerebellar cortex might not therefore be explained by differences in VMAT2 protein itself but possibly by other imaging variables, for example, partial volume effects.

Keywords: cerebellum, occipital cortex, positron emission tomography, reference region, substantia nigra, vesicular monoamine transporter 2

Introduction

The vesicular monoamine transporter 2 (VMAT2), a member of the solute carrier family 18 (SLC18A2) with 12 transmembrane domains, is the protein responsible for transporting monoamine neurotransmitters (dopamine, noradrenaline, serotonin) into synaptic vesicles for subsequent storage and release (Erickson et al, 1996). Tetrabenazine or its metabolite dihydrotetrabenazine (DTBZ) is a specific inhibitor of the transporter used in radioligand-binding studies to measure the in vitro concentration of VMAT2 in mammalian brain (Scherman et al, 1988; Darchen et al, 1989; Vander Borght et al, 1995; Wilson et al, 1996). Positron emission tomography (PET) imaging of [¹¹C]DTBZ binding has now been developed as a noninvasive tool to estimate brain levels of VMAT2 in vivo (Kilbourn et al, 1993; Frey et al, 1996; Chan et al, 1999; Koeppe et al, 1999), in particular in the striatum (caudate and putamen) in which most of VMAT2 (90% to 95%) is considered to be localized to the dopamine nerve terminals (cf. Boileau et al, 2008). The advantage of VMAT2 imaging over other presynaptic dopamine terminal markers including the decarboxylase (by ¹⁸F-DOPA) and the dopamine transporter (e.g., by ¹¹C-methylphenidate) is the insensitivity of VMAT2 protein to several dopaminergic modulations (cf. Boileau et al, 2008; Tong et al, 2008) although it was recently demonstrated that in vivo [¹¹C]DTBZ uptake could be influenced by presumably large changes in vesicular dopamine levels (see Boileau et al, 2010; Kilbourn et al, 2010 for references and discussion). Positron emission tomography VMAT2 imaging has been commonly used to assess striatal dopamine terminal integrity in a variety of human neuropsychiatric conditions, for example, in Parkinson's disease (PD) (Frey et al, 1996; Brooks et al, 2003; Okamura et al, 2010).

A practical issue with PET VMAT2 imaging is selection of a reference region for the measurement of free and nonspecific binding (Chan et al, 1999). In principle, an ideal reference region should have identical free and nonspecific properties as compared with regions of interest with specific binding, a reasonably high uptake of the radiotracer, and a negligible contribution of specific binding to the tracer uptake in this region, that is, very low levels of the protein target. Among the PET VMAT2 studies, the selection of reference region has been arbitrary, with cerebellar (Chan et al, 1999), whole cerebral (Gilman et al, 1998, 1999), frontal (Frey et al, 1996; Gilman et al, 1996), parieto-occipital (Troiano et al, 2010), or occipital cortices (Chan et al, 1999; Koeppe et al, 1999; Boileau et al, 2008; Okamura et al, 2010) having been used. Currently, the occipital cortex appears to be a preferred reference region in human brain imaging studies apparently because the data obtained are less variable and of consistently higher values as compared with those using cerebellar cortex as the reference region (Chan et al, 1999). However, animal studies have repeatedly showed that the cerebellar cortex contains less DTBZ-binding sites than cerebral cortices (Scherman et al, 1988; Darchen et al, 1989; Vander Borght et al, 1995) and in the development of new DTBZ-based PET tracers the cerebellum, for practical reasons, continues to be used as the reference region in preclinical studies (Kilbourn et al, 2007).

To address the question of the most appropriate reference region for VMAT2 PET imaging, we reasoned that it would be helpful to know the relative concentrations of VMAT2 protein in different regions of the human brain. The current study therefore used quantitative VMAT2 immunoblotting in autopsied human brain. Given that several VMAT2-immunoreactive species of uncertain identity have been reported in the literature (Wang et al, 1997; Jassen et al, 2005; Cruz-Muros et al, 2008), we also made efforts to confirm these VMAT2 Western blot bands.

Materials and methods

Subjects

This study was approved by the Research Ethics Board of the Centre for Addiction and Mental Health at Toronto. A total of six (4M/2F) autopsied brains from neurologically normal subjects (age: 48±0.3 years; postmortem interval: 16±3

hours; mean±s.e.m.) were used in the VMAT2 regional distribution study. To help characterize the VMAT2-immunoreactive protein bands (which should be reduced in intensity in degenerative nigrostriatal dopamine deficiency disorders) in the Western blots, autopsied brains were also obtained from a total of 10 patients with PD (5M/5F), 10 patients with progressive supranuclear palsy (PSP; 9M/1F), 9 patients with multiple system atrophy (MSA; 5M/4F), and 10 normal subjects (4M/6F). No significant difference (one-way analysis of variance) was found among the four groups in postmortem interval (control: 12±1; PD: 14±2; PSP: 11±2; MSA: 14±2

hours) or in age (control: 70±3; PD: 75±2, PSP: 73±4; MSA: 64±4 years). One-half brain was used for neuropathological examination, whereas the other half was frozen for neurochemical analyses. The characteristics and pathological findings of the patients have been previously published (Tong et al, 2010). The causes of death for the neuropathologically normal control subjects were cardiovascular illnesses (n=12), bronchopneumonia (n=2), pulmonary edema (n=1), and trauma (n=1).

Tissue Sample Preparation, Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis, and Western Blotting

Cerebral cortical subdivisions were excised according to Brodmann classification. Dissection of the subcortical areas from the ~3

mm thick coronal sections followed published procedures (Kish et al, 2005; Tong et al, 2010). Brain tissue homogenates were used throughout this study. Sample preparation, sodium dodecyl sulfate polyacrylamide gel electrophoresis, and Western blot followed published procedures (Kish et al, 2005). Protein concentration was determined using the Bio-Rad Protein Assay Kit (Bio-Rad, Hercules, CA, USA) with bovine plasma albumin as the standard. Five concentrations of tissue standard (0.25 to 3

μg of protein), consisting of a pooled human striatal samples, were run on each blot together with the samples (2 to 40

μg of protein, depending on regional levels of VMAT2; see Figure 4). As VMAT2 has a strong propensity to aggregate during heating or long-term storage in sodium dodecyl sulfate polyacrylamide gel electrophoresis sample buffer in the freezer, the samples were prepared by incubation at room temperature for 30

minutes immediately before use. The antibody used for quantitative determination of VMAT2 levels was a well-characterized (Miller et al, 1999; Haycock et al, 2003) rabbit polyclonal antibody raised against the 19-amino-acid C-terminus peptide of human VMAT2 (AB1767; Chemicon International, Temecula, CA, USA). For measurement of the level of the ~35

kDa VMAT2 band IV (see Figures 1, ,2,2, ,33 and and4),4), blots were cut between the two colored molecular weight (MW) markers (47.5 and 32.5

kDa), with the upper and lower half probed with the primary antibody at a dilution of 1:10,000 and 1:3,000, respectively. The same dilution of 1:10,000 was used for the goat anti-rabbit IgG (H+L) horseradish peroxidase secondary antibody (cat# 4050-05; SouthernBiotech, Birmingham, AL, USA). Another recently available anti-human VMAT2 monoclonal antibody (clone 9E11, cat# TA500506, OriGene Technologies, Rockville, MD, USA) raised against the recombinant, full-length VMAT2 protein was also used to help characterize the VMAT2-immunoreactive protein bands. Western blot of the ‘control' protein neuron specific enolase followed published procedures (Kish et al, 2005).

Figure 1

Western blotting of vesicular monoamine transporter 2 (VMAT2) in the striatum of autopsied human brain. (A) Representative immunoblots of the tissue standard comprised of pooled human putamen and caudate samples probed with the rabbit polyclonal antibody (more ...)

Figure 2

Vesicular monoamine transporter 2 (VMAT2) band IIb in the substantia nigra and neighboring regions of autopsied human brain. (A) Representative immunoblots with AB1767 in the substantia nigra pars compacta (SNpc; 10

μg), red nucleus (RN; (more ...)

Figure 3

Vesicular monoamine transporter 2 (VMAT2) in the brain of patients with Parkinson's disease (PD), progressive supranuclear palsy (PSP), and multiple system atrophy (MSA). (A) Immunoblots (AB1767) of putamen and caudate (2

μg) of PD patients (more ...)

Figure 4

Representative immunoblots of vesicular monoamine transporter 2 (VMAT2) in 37 brain regions of a normal control subject. ‘A' refers to Brodmann cerebral cortical areas; A23, cingulate gyrus posterior; A24, cingulate gyrus anterior; A25, parolfactory (more ...)

Data Analyses

Four linear standard curves were constructed for each of the VMAT2 protein bands (I, IIa, III, and IV; see Figure 1B for an example) on each blots. The relative levels of the four VMAT2 protein bands in the tissue standard were determined by ratios of the slopes of the standard curves of band IIa, III, and IV to that of band I, that is, R_IIa/I, R_III/I, and R_IV/I, which were measured in blots probed with one concentration of the primary antibody (Figures 1A and 1B). The concentration of each protein band in a sample was determined by interpolation from the respective standard curves, with levels of band II_a/b, III, and IV then standardized against band I by multiplying the ratio R, and the results were expressed as μg tissue standard (band I)/μg sample protein. The within and between blots coefficient of variation for each VMAT2 protein bands were 4.9% and 14.2% for band I, 7.7% and 11.7% for band II_a/b, 9.6% and 14.4% for band III, and 8.5% and 10.7% for band IV, respectively. For simplicity only, VMAT2 ‘immunoreactivity' will be referred to as ‘levels.'

Differences in VMAT2 levels among control and parkinsonian patients were assessed by one-way analysis of variance followed by post hoc Bonferroni adjustments. Differences in percentage loss versus control among the five VMAT2 bands in the substantia nigra of parkinsonian patients were assessed by repeated measures analysis of variance. Vesicular monoamine transporter 2 regional distribution was examined by one-way repeated measures analysis of variance followed by post hoc Bonferroni adjustments. No significant correlation was observed between the levels of VMAT2 proteins and postmortem interval of the subjects examined.

Results

Characterization of Vesicular Monoamine Transporter 2-Immunoreactive Protein Bands in Western Blots of Normal Autopsied Human Striatum

The polyclonal antibody raised against a 19-amino-acid C-terminus peptide of human VMAT2 (AB1767 from Chemicon) has been widely used to examine VMAT2 protein by Western blot in striatum of human brain (Miller et al, 1999; Haycock et al, 2003; Little et al, 2003) and in the brain of rodents under a variety of conditions (Wang et al, 1997; Gainetdinov et al, 1998; Cruz-Muros et al, 2008). As expected (Haycock et al, 2003), the antibody detected in human striatal samples a total of four broad protein bands with an MW of ~75 (I), 55 (IIa), 45 (III), and 35

kDa (IV), respectively (Figure 1A). The ~75

kDa VMAT2 I was the predominant band, accounting for 53%±2% (n=10) and 56%±3% (n=10) of the total VMAT2 immunoreactivity in putamen and caudate, respectively. The intensity of the smaller bands decreased along with their sizes in the putamen (28%±1%, 14%±1%, and 5%±1% of total, respectively; see Figure 2B) and caudate (26%±2%, 14%±1%, and 5%±1% of total, respectively). The ~35

kDa band IV was either ignored (Haycock et al, 2003; Jassen et al, 2005) or not reported (Miller et al, 1999) in previous studies due to its weak staining.

To address the literature controversy with respect to the identity of the multiple VMAT2-immunoreactive bands detected in the striatum (Wang et al, 1997; Gainetdinov et al, 1998; Miller et al, 1999; Jassen et al, 2005; Cruz-Muros et al, 2008), we performed immunoblotting with another recently available monoclonal antibody (9E11) raised against recombinant, full-length human VMAT2. As shown in Figure 1A, the monoclonal antibody detected in human striatal samples only the two larger forms (I and IIa) of VMAT2, with the relative intensity of the two bands also similar to that observed with the polyclonal antibody. Therefore, our observations with two antibodies against different epitopes of VMAT2 provide some support for the notion that the minor VMAT2-immunoreactive species at 45 and 35

kDa might be N-terminally truncated forms (Wang et al, 1997; Gainetdinov et al, 1998; Miller et al, 1999; Jassen et al, 2005).

Distinct Vesicular Monoamine Transporter 2 Immunoreactivity in the Normal Human Substantia Nigra

The four-band pattern (I, IIa, III, and IV) of VMAT2 detected with the polyclonal antibody was generally observed throughout the human brain regions examined (see Figure 4). However, we found that another protein band (~52

kDa, IIb) of slightly smaller MW than the 55-kDa band could be detected by the polyclonal antibodies in substantia nigra pars compacta, the neighboring midbrain regions red nucleus and subthalamic nucleus, and in the internal portion of the globus pallidus (GPi) (Figure 2A, see also Figures 3B and and4).4). Moreover, in the substantia nigra, the 52-kDa band IIb was of (at least) comparable intensity as that of the mature 75

kDa band I (35%±1% versus 35%±2% of total levels of VMAT2, respectively; Figure 2B), with levels of the 55-kDa band IIa low in the dopamine cell body region (9%±1%). The 75-kDa band I nevertheless was predominant in other brain regions. The percentage levels of band I, IIa, and IIb were 54%±2%, 15%±1%, and 13%±2% in the red nucleus, 39%±3%, 21%±2%, and 20%±1% in the subthalamic nucleus, and 41%±2%, 22%±1%, and 13%±1% in the GPi. We also confirmed that the 52-kDa band IIb in the substantia nigra was immunoreactive to the monoclonal antibody (Figure 2C).

Vesicular Monoamine Transporter 2 Immunoreactivity Is Decreased in Substantia Nigra and Striatum of Patients with Degenerative Parkinsonian Conditions

To provide further evidence that the protein bands (I, IIa, IIb, III, and IV) detected by the antibodies were in fact derived from VMAT2, we examined brain of patients with PD, the classic degenerative dopamine, and VMAT2 deficiency condition (Frey et al, 1996; Wilson et al, 1996). As expected, in the striatum (putamen and caudate) of patients with PD, levels of all four VMAT2 bands I, IIa, III, and IV were markedly and evenly decreased, with the loss significantly more severe in putamen (−92%±1%, −90%±2%, −86%±2%, and −79%±2% for bands I to IV, respectively) than in caudate (−73%±8%, −75%±6%, −56%±3%, and −70%±5%, respectively; Figures 3A and 3C). The overall loss of VMAT2 immunoreactivity was −90%±2% and −71%±6% for putamen and caudate, respectively, consistent with the literature showing that the putamen has more severe dopamine marker loss in PD than the caudate (Frey et al, 1996; Wilson et al, 1996). Similarly in the substantia nigra, levels of all five VMAT2-immunoreactive bands were significantly reduced (−47%±4%, −55%±4%, −84%±2, −60%±3, and −60%±8%, respectively) in PD although to a less overall extent (−63%±3%) than those in the striatal terminal regions (Figures 3B and 3C). Again, this is in line with the pathological finding that the loss of dopamine cell body is less severe than the loss of dopamine terminals in the striatum in PD (cf. Hornykiewicz, 1998). Interestingly, we found that the 52-kDa band IIb was preferentially lost as compared with the other bands (−84% versus −47% to −60% P<0.05) in the substantia nigra of PD, with the extent of loss similar to that observed in the striatum (Figures 3B and 3C). Similar findings were observed in the substantia nigra of patients with PSP (−62%±4%, −62%±6%, −88%±3%, −76%±3%, and −75%±3% for bands I to IV, respectively) and MSA (−51%±6%, −43%±12%, −77%±6%, −68%±5%, and −59%±7%, respectively), two atypical parkinsonian conditions characterized, in part, by degeneration of nigrostriatal dopamine neurones. The overall loss of nigral VMAT2 protein was slightly higher in PSP (−74%±3%) than in MSA (−63%±6%) or in PD (−63%±3%).

Levels of neuron specific enolase, a control protein, were normal in the putamen, caudate, and substantia nigra of patients with PD (Figures 3A and 3B). However, neuron specific enolase levels in the substantia nigra were significantly decreased in patients with PSP (−47%) and MSA (−33%).

Regional Distribution of Vesicular Monoamine Transporter 2 in Normal Human Brain

A total of 37 brain regions covering the entire human forebrain and including both primarily dopaminergic and nondopaminergic areas were examined (see Figure 4 for representative immunoblots). It should be mentioned that with higher loading of protein, additional protein bands including some high MW bands and two sharp bands immediately above and below the 35-kDa VMAT2 band IV could be observed with the polyclonal antibody, particularly in thalamus and cerebral cortices. However, these protein bands appeared to be nonspecific reaction products as, unlike VMAT2 proteins, they were enriched in the soluble fraction. All four or five VMAT2 protein bands were measured quantitatively and normalized against putamen, the brain region with highest levels of VMAT2. As shown in Figure 5, the regionally normalized data for levels of mature VMAT2 (band I), intact VMAT2 (bands I+II, including both IIa and IIb), and total VMAT2 were not significantly different (region × measure: F_72,540=0.095, P=1.0), suggesting that the regional difference (F_36,540=90.3, P<0.0001) in the levels of the different VMAT2 bands were proportional (except for band IIb in substantia nigra; see above). Therefore, in the following description of the results, the regional pattern of total VMAT2 versus that of the individual species was highly similar.

Figure 5

Regional brain distribution of vesicular monoamine transporter 2 (VMAT2) in normal human brain (n=6). Shown are normalized levels (mean±s.e.m.) of total (bands I–IV), intact (bands I–IIa/b), and mature (band I only) VMAT2 against (more ...)

The brain gray matter regions could be separated into four groups according to VMAT2 protein levels. The highest levels of VMAT2 were in the striatum and were at least threefold higher than that of any other brain region. Concentrations of VMAT2 in putamen were somewhat higher than in caudate although the difference was not statistically significant. The basal forebrain regions including nucleus basalis, hypothalamus, and substantia innominata and the midbrain regions substantia nigra pars compacta and red nucleus had moderate concentrations of VMAT2 that were 20% to 30% of that of putamen. The other subcortical brain structures together with a few allocortical/archicortical areas, including the basal ganglia output regions subthalamic nucleus, globus pallidus external (GPe) and GPi, the medial temporal lobe regions gyrus of uncus, dentate gyrus, Ammon's horn, amygdala, and hippocampal gyrus, the thalamic subregions medial pulvinar, lateral ventral, anterior ventral, mediodorsal, and lateralis, and the parolfactory cortical area A25 contained low levels of VMAT2 (1% to 10% of putamen).

All of the cerebral neocortical areas and cerebellar cortex had a level of mature or intact VMAT2 that was <1% of that of the striatum (Figure 5, inset). No statistically significant difference was observed among these brain regions, in particular between cerebellar and occipital cortex, although there were trends for the cingulate (A24 and A23) and insular cortices to have relatively higher levels of VMAT2 than other neocortical regions. The cerebral white matters, including the corpus callosum and internal capsule, had the lowest levels of VMAT2 (<0.25% of putamen) that were barely detectable.

In contrast to the above (Figure 5), the house-keeping protein neuron specific enolase had a different brain distribution, with levels quite homogenous across the gray matter regions but, as expected, low in white matter, a finding consistent with our previous reports (Kish et al, 2005).

Given that VMAT2 is localized to dopamine, noradrenaline, and serotonin neurons, we reasoned that VMAT2 protein levels in the different brain areas (having variable dopamine–noradrenaline–serotonin ratios) might show a good correlation with the sum of the concentration of the three neurotransmitters. Using regional brain monoamine neurotransmitter data reported in our previous studies (a total of 27 brain regions; Tong et al, 2007), we found a positive correlation (Pearson) between protein levels of VMAT2 and tissue concentrations of total monoamines neurotransmitters (dopamine, noradrenaline, and serotonin; r=0.99 or 0.95, with or without the two striatal outlier points, respectively; P<0.0001). This correlation was not as strong using dopamine concentrations alone (r=0.96 or 0.77, with or without the two striatal outlier points, respectively; Figure 6).

Figure 6

Correlation (Pearson) between levels of vesicular monoamine transporter 2 (VMAT2) protein and tissue concentrations of total monoamines (dopamine, noradrenaline, and serotonin) or dopamine (DA) alone among brain regions examined (n=27). The inset shows (more ...)

In our samples of normal subjects (n=10 to 16), we did not observe any significant correlation (Pearson) between the levels of VMAT2 protein and the age of the subjects (47 to 80 years) in the putamen (r=−0.32, P=0.36), caudate (r=−0.14, P=0.71), or substantia nigra (r=0.21, P=0.44).

Discussion

To our knowledge, this is the first detailed quantitative examination of VMAT2-immunoreactive species in different human brain regions. The major finding from a practical neuroimaging perspective is that the levels of VMAT2 protein in the cerebellar cortex and the widespread cerebral neocortical areas were all at least 100-fold lower than those in striatum. This supports the usefulness of these areas as potential reference regions for PET imaging of VMAT2 in the human. We also found that the human substantia nigra was different in VMAT2 immunoreactivity from the dopamine terminal regions by containing a distinct dominant 52

kDa band IIb that was preferentially lost in parkinsonian conditions.

The multiple bands (I, IIa, IIb, III, IV) of VMAT2 detected by the commonly used C-terminus antibodies (AB1767 used in this study and another similar antibody AB1598P from Chemicon, initially developed by Erickson et al (1996) and Edwards and colleagues (Peter et al, 1995), respectively) and their uncertain identity have been a major challenge for quantitative studies of VMAT2 protein in the brain. Many studies thus chose to examine only the major band detected (Sandoval et al, 2002; Haycock et al, 2003; Little et al, 2003; Gonzalez-Hernandez et al, 2004; Fortune and Lurie, 2009), which in most cases has been the mature band I (Wang et al, 1997; Miller et al, 1999; Haycock et al, 2003; Little et al, 2003; Gonzalez-Hernandez et al, 2004; Jassen et al, 2005; Cruz-Muros et al, 2008) although in a few instances a 55-kDa band was found to be the major band in rodent brains (Holtje et al, 2003; Duchemin et al, 2009; Fortune and Lurie, 2009). The reason for the inconsistencies is not clear but could arise from differential sample preparation, particular Western blot conditions and antibody used, and/or the batch-to-batch variance of the antibodies. We have used four batches of the same version (AB1767) of the rabbit polyclonal antibodies against the C-terminus 19 amino acids of human VMAT2 from different commercial sources and have obtained the same results as observed by most of the studies, that is, the 75-kDa band I was dominant but accounted for only ~50% of total VMAT2 immunoreactivity in the striatum.

In the present study, by using a newly available monoclonal antibody, by examining multiple brain regions, and by including brain of patients with degenerative parkinsonism, we established that (1) the minor smaller bands III and IV are most likely N-terminal truncated forms of VMAT2; (2) the banding pattern described above in the striatum (I, IIa, III, IV) also applies to most of the monoamine terminal fields except in the nigral dopamine cell body region (and its close proximity regions including red nucleus, subthalamic nucleus, and GPi), in which a band (IIb) of slightly smaller MW than that of band IIa could be detected; and (3) the overall extent of loss of VMAT2 immunoreactivity in the brain regions of patients with PD was correlated with the expected degree of dopaminergic neuronal damage (putamen>caudate>substantia nigra). An exception is band IIb in substantia nigra, with the extent of loss similar to that observed in the terminal regions caudate and putamen. This interesting finding, together with the limited distribution of band IIb in human brain that might be related to the distance from the dopamine cell body (e.g., GPi versus GPe), suggest that VMAT2 IIb might be the precursor to IIa and I being posttranslationally modified and transported to the terminals. However, we cannot exclude the possibility that bands I/IIa/b might represent the transporter associated with different monoaminergic neurons or different subcellular structures, for example, small synaptic vesicles versus large dense core vesicles (Nirenberg et al, 1996). The lack of an aging change of VMAT2 protein in the nigrostriatal system in our limited sample size and within a limited age span is nonetheless consistent with a previous Western blotting study (Haycock et al, 2003) and a recent PET VMAT2 imaging report (Troiano et al, 2010) although the question of the influence of aging on striatal VMAT2 in the human is still controversial (cf. Troiano et al, 2010).

Notwithstanding the generic limitation of Western blot investigations that bands with different MW could well be differentially transferred and detected, we attempted to measure the levels of total, intact, and mature VMAT2 proteins. As the tetrabenazine-binding site on VMAT2 has been mapped to the juxtaposed N- and C-terminals (Sievert and Ruoho, 1997), in particular a conserved phenylalanine (F135 of hVMAT2) in the second transmembrane domain (Gros and Schuldiner, 2010) and glycosylation does not appear to influence the transporter's activity and binding of tetrabenazine (Yelin et al, 1998), the VMAT2 proteins that are capable of binding DTBZ might include all the intact species, that is, I, IIa, and IIb, but not the N-terminally truncated III and IV. However, since the distribution patterns of total, intact, and mature VMAT2 immunoreactivity were all similar, possibly differential DTBZ-binding capacity of the different VMAT2 species should reasonably not affect the regional pattern of DTBZ binding (except, possibly, in the substantia nigra due to the unique abundance of VMAT2 IIb). Indeed, the overall brain distribution of VMAT2 proteins was generally consistent with in vivo PET imaging data with (+)-[¹¹C]DTBZ (Koeppe et al, 1999) and with findings of the in vitro radioligand-binding study of autopsied human brain by [³H]DTBZ (Scherman et al, 1988), showing striatum [dbl greater-than sign]

substantia nigra (midbrain), hypothalamus>globus pallidus, hippocampus, thalamus>cerebral cortices (occipital and cerebellar cortices were not measured in the Scherman study).

However, one notable difference between the results of our in vitro measurement of VMAT2 protein and the in vivo PET imaging of [¹¹C]DTBZ binding was in cerebellum. Whereas we did not observe any difference in VMAT2 protein levels between the cerebellar cortex and the cerebral neocortices, PET imaging detects small but consistently higher binding in the cerebellum or cerebellar cortex (Kilbourn et al, 1993; Chan et al, 1999; Koeppe et al, 1999). Therefore, the cerebral cortical regions, in particular the occipital cortex rather than cerebellar cortex, have been the preferred reference region for PET VMAT2 imaging. One possible explanation for the in vitro versus in vivo ‘discrepancy' is that VMAT2 protein levels determined in autopsied brains might not correspond to that available for binding of the radioligand in vivo due to a variety of uncertain factors including differential subcellular localization and posttranslational modification status of the transporter, for example, phosphorylation. Possible differences in nonspecific binding profiles of the tracer among different brain regions could also be a contributing factor. Alternatively, differences in VMAT2 binding between cerebellar and cerebral cortices as assessed by PET imaging might be explained by other imaging variables, for example, partial volume effects. The cerebral and cerebellar cortices are quite different in anatomical structure, with the former having a much smaller surface area/volume ratio and lower percentage of surface area hidden within fissures than the latter (Henery and Mayhew, 1989), which could cause more marked partial volume effects and artificially lower binding in cerebral versus cerebellar cortices. In this regard, previous PET studies of [¹¹C]flumazenil (Aston et al, 2002) and [¹¹C]-arachidonic acid (Giovacchini et al, 2004) brain uptake have disclosed a larger effect of partial volume correction in most regions of the cerebral cortices than in the cerebellar cortex.

In conclusion, although no cerebral gray matter region in the human brain is free of VMAT2 protein, levels of the transporter in the cerebral and cerebellar cortex were sufficiently low to satisfy this criterion as a reference region in PET VMAT2 imaging. We also found that the cerebellar cortex and the (now preferred reference region) occipital cortex were not different in VMAT2 protein concentrations. Differences in PET VMAT2 binding between the two regions might in this respect provide an example of bias caused by imaging variables such as partial volume effects in the reference region.

Notes

The authors declare no conflict of interest.

Footnotes

This study was supported by USA NIH NIDA DA025096, the Friedman MSA Fund, the William S Storey and Al Silverberg PSP Funds, the CAMH PSP Fund, and the Abe Memorial Fund.

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