PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of narLink to Publisher's site
 
Nucleic Acids Res. 1982 June 25; 10(12): 3617–3626.
PMCID: PMC320739

Initiation of transcription of genes for mitochondrial ribosomal RNA in yeast: comparison of the nucleotide sequence around the 5'-ends of both genes reveals a homologous stretch of 17 nucleotides.

Abstract

The DNA sequence around the beginning of the genes coding for the large and small ribosomal RNAs in yeast mitochondria has been established. In order to determine the 5'-end points of the ribosomal RNAs, DNA fragments were labelled in vitro at a restriction site within each gene and hybridized with ribosomal RNA. The hybrids were then treated with S1 nuclease and the products analysed for size by gel electrophoresis. This enabled us to identify where in the determined DNA sequence the 21S ribosomal RNA and the precursor for 15S ribosomal RNA (15.5S rRNA) start, since both transcripts are initiated de novo (Levens et al. (1981) J.Biol.Chem., 256, 5226-5232). Comparison of the DNA sequences around the start points of transcription reveals the existence of a homologous stretch of 17 nucleotides. This conserved sequence may be an essential element of a promoter in mtDNA.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (910K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Borst P, Grivell LA. The mitochondrial genome of yeast. Cell. 1978 Nov;15(3):705–723. [PubMed]
  • Levens D, Ticho B, Ackerman E, Rabinowitz M. Transcriptional initiation and 5' termini of yeast mitochondrial RNA. J Biol Chem. 1981 May 25;256(10):5226–5232. [PubMed]
  • Osinga KA, Evers RF, Van der Laan JC, Tabak HF. A putative precursor for the small ribosomal RNA from mitochondria of Saccharomyces cerevisiae. Nucleic Acids Res. 1981 Mar 25;9(6):1351–1364. [PMC free article] [PubMed]
  • Ohnishi T, Kawaguchi K, Hagihara B. Preparation and some properties of yeast mitochondria. J Biol Chem. 1966 Apr 25;241(8):1797–1806. [PubMed]
  • Moorman AF, Grivell LA, Lamie F, Smits HL. Identification of mitochondrial gene products by DNA-directed protein synthesis in vitro. Biochim Biophys Acta. 1978 Apr 27;518(2):351–365. [PubMed]
  • Arnberg AC, Van Ommen GJ, Grivell LA, Van Bruggen EF, Borst P. Some yeast mitochondrial RNAs are circular. Cell. 1980 Feb;19(2):313–319. [PubMed]
  • Meagher RB, Tait RC, Betlach M, Boyer HW. Protein expression in E. coli minicells by recombinant plasmids. Cell. 1977 Mar;10(3):521–536. [PubMed]
  • Maxam AM, Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. [PubMed]
  • Maat J, Smith AJ. A method for sequencing restriction fragments with dideoxynucleoside triphosphates. Nucleic Acids Res. 1978 Dec;5(12):4537–4545. [PMC free article] [PubMed]
  • Berk AJ, Sharp PA. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977 Nov;12(3):721–732. [PubMed]
  • Sollner-Webb B, Reeder RH. The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis. Cell. 1979 Oct;18(2):485–499. [PubMed]
  • Merten S, Synenki RM, Locker J, Christianson T, Rabinowitz M. Processing of precursors of 21S ribosomal RNA from yeast mitochondria. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1417–1421. [PubMed]
  • Tabak HF, van der Laan J, Osinga KA, Schouten JP, van Boom JH, Veeneman GH. Use of a synthetic DNA oligonucleotide to probe the precision of RNA splicing in a yeast mitochondrial petite mutant. Nucleic Acids Res. 1981 Sep 25;9(18):4475–4483. [PMC free article] [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press