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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Contrast Media Mol Imaging. Author manuscript; available in PMC 2012 September 1.
Published in final edited form as:
PMCID: PMC3205414
NIHMSID: NIHMS283933

Photoacoustic tomography: fundamentals, advances and prospects

Abstract

Optical microscopy has been contributing to the development of life science for more than three centuries. However, due to strong optical scattering in tissue, its in vivo imaging ability has been restricted to studies at superficial depths. Advances in photoacoustic tomography (PAT) now allow multiscale imaging at depths from sub-millimeter to several centimeters, with spatial resolutions from sub-micrometer to sub-millimeter. Because of this high scalability and its unique optical absorption contrast, PAT is capable of performing anatomical, functional, molecular and fluid-dynamic imaging at various system levels, and is playing an increasingly important role in fundamental biological research and clinical practice. This Review discusses recent technical progress in PAT and presents corresponding applications. It ends with a discussion of several prospects and their technical challenges.

Keywords: Photoacoustic tomography, photoacoustic microscopy, photoacoustic computed tomography, photoacoustic endoscopy, multiscale imaging, optical absorption contrast

1. Introduction

By zooming in on tiny features, optical microscopy has driven the development of life science. However, no matter how sophisticated the optical microscope, its imaging signal fades exponentially with imaging depth (1,2). As a photon travels through tissue, multiple scattering events eventually lead to randomization of its propagation direction, i.e., photon diffusion, and thus blur the image (3). The mean propagation distance for a photon to diffuse is termed the transport mean free path (TMFP), which is typically 1 mm in tissue (2). No existing optical microscopy technologies can penetrate beyond one TMFP, where many biological processes and diseases occur. Therefore, there is a need for non-invasive in vivo imaging with high resolution in deep tissue.

Photoacoustic tomography (PAT), an emerging powerful optical imaging modality using optical absorption contrast and ultrasonic resolution, has broken through the fundamental barrier of one TMFP imaging depth (2,49). Most importantly, all the key characteristics of PAT are highly scalable. PAT has become one of the fastest growing fields in biomedical imaging (7). To avoid overlapping with recent Review articles, this Review mostly focuses on progress since the second half of year 2009. It is organized in the following order: (1) fundamentals of the photoacoustic effect and photoacoustic imaging, (2) multiscale PAT systems, based on their image formation mechanisms, (3) anatomical and functional PAT using intrinsic contrasts, (4) molecular and chemical PAT using exogenous contrasts, (5) PAT of fluid dynamics, and (6) prospects and challenges for PAT development in the near future.

2. Fundamentals of photoacoustic tomography

Based on the photoacoustic effect, discovered by Alexander G. Bell in 1880 (2), the principle of PAT is illustrated in Fig. 1. Typically, the PA effect starts from a target within tissue irradiated by a short laser pulse. The pulse energy is partially absorbed by the target and converted into heat, which generates a local transient temperature rise, followed by a local pressure rise through thermo-elastic expansion. The pressure propagates as ultrasonic waves, termed PA waves, and is detected by ultrasonic transducers placed outside the tissue. A PA image is then formed by resolving the origins of the ultrasonic waves from their arrival times. Because the PA signal amplitude is proportional to the product of the local absorption coefficient and local fluence, PAT is essentially listening to the optical absorption contrast of tissue. Meanwhile, because PAT uncouples signal generation and detection, the diffused photons also contribute to the resultant PA signals without degrading the signal quality. For biological tissue, because ultrasonic scattering is about two to three orders of magnitude weaker than optical scattering, PAT can achieve high spatial resolution deep in tissue. In addition, unlike other coherent imaging techniques, PAT is speckle free (10).

Figure 1
Illustration of the photoacoustic (PA) effect and PA imaging

3. Multiscale photoacoustic tomography systems

From organelles to organs, currently, PAT is the only imaging modality spanning the microscopic and macroscopic worlds. The high scalability of PAT is achieved by trading off imaging resolutions and penetration depths (11). Higher acoustic frequency contributes to higher spatial resolution, but is attenuated more by tissue, thus resulting in a shallower penetration depth, and vice versa. In addition, optical attenuation is another limiting factor for penetration depth, since PA waves are generated only where photons can reach. According to their imaging formation mechanisms, PAT systems can be classified into four categories: raster-scan based photoacoustic microscopy (PAM), inverse-reconstruction based photoacoustic computed tomography (PACT), rotation-scan based photoacoustic endoscopy (PAE), and hybrid PAT systems with other imaging modalities. A thorough side-by-side comparison of different PAT systems can be found in recent Review articles (9,11).

3.1. Raster-scan based photoacoustic microscopy

By using a single focused ultrasonic transducer, usually placed confocally with the irradiation laser beam, PAM forms a 1D image at each position, where the flight time of the ultrasound signal provides depth information. A 3D image is then generated by piecing together the 1D images obtained from raster scanning, and thus no inverse reconstruction algorithm is needed. PAM has two forms, based on its focusing mechanism. In acoustic-resolution photoacoustic microscopy (AR-PAM), the optical focus is usually expanded wider than the acoustic focus, and thus acoustic focusing provides the system resolution [Fig. 2(A)] (12,13). Because the resolution is not affected by optical scattering, by using a focused ultrasonic transducer with a 50-MHz central frequency and a 70% nominal bandwidth, a transverse resolution of 15 μm and axial resolution of 45 μm have been achieved with a maximum penetration depth of 3 mm in live animals. However, to further improve the resolution by increasing the acoustic frequency is quite challenging, because of the strong acoustic attenuation at high frequencies (14). The other form of PAM, termed optical-resolution photoacoustic microscopy (OR-PAM), has an optical focus much tighter than the acoustic focus, and thus the system resolution is provided by optical focusing. Since the optical wavelength is much shorter than the acoustic wavelength, OR-PAM can easily achieve high spatial resolution, down to the micrometer or even sub-micrometer scale [Fig. 2(B)] (14,15). However, like traditional optical microcopy, OR-PAM generally obeys the one TMFP limitation due to photon scattering, which, however, is still deeper than the acoustic solution mentioned above.

Figure 2
Raster-scan based photoacoustic microscopy (PAM)

PAM usually suffers from slow imaging speed due to raster scanning. To improve this, different scanning mechanisms have been proposed to replace the traditional mechanical scanning. These include optical scanning using Galvo mirrors (~2 Hz frame rate) (16), mechanical scanning using a voice-coil motor (~15 Hz frame rate) (17), and hybrid scanning with optical scanning on one axis and mechanical scanning on the other axis (~6 Hz frame rate) (18).

3.2. Inverse-reconstruction based photoacoustic computed tomography

Despite its high spatial resolution and improved imaging speed, PAM usually has a limited focal depth and is not yet capable of video-rate imaging (19). In contrast, PACT is typically implemented using full-field illumination and a multi-element ultrasound array system to improve penetration depth and imaging speed [Fig. 3(A)] (2024), though some PACT systems use a single-element transducer with circular scanning [Fig. 3(B)] (25). The spatial distribution of acoustic sources needs to be inversely reconstructed. The ultrasound array can be fabricated in different geometrical forms such as circular (20), semi-circular (23), quarter-circular (26), hemisphere (24), linear (22), and square (27), depending on the application. Mainly determined by the laser repetition rate and data acquisition speed, PACT has been reported with a cross-sectional frame rate of up to 50 Hz (11). Meanwhile, the penetration depth can reach several tens of TMFPs in live tissue. The imaging resolution is usually compromised to several hundreds of micrometers due to the low ultrasound frequency used.

Figure 3
Inverse-reconstruction based photoacoustic computed tomography (PACT)

In addition to PACT implementations using piezoelectric ultrasonic transducers, there has been a growing interest in detecting PA signals using optical methods (2832). Optical detection can potentially improve the imaging sensitivity and eliminate the coupling medium between the sample surface and the ultrasonic transducer (28,31).

3.3. Rotation-scan based photoacoustic endoscopy

Even though the penetration depth of PACT can reach several centimeters, internal organs such as the cardiovascular system and gastrointestinal tract are still not reachable. Non-invasive tomographic imaging of these internal organs is extremely useful in clinical practice. Besides pure optical and ultrasound endoscopy (3335), photoacoustic endoscopy (PAE) is another promising solution for this clinical need (6). The key specifications of PAE are the probe dimensions and imaging speed. The first PAE was designed by Yang et al., and applied to animal studies (Fig. 4) (36). Here, the PAE probe has a diameter of 4.2 mm and the cross-sectional scanning speed is 2.6 Hz.

Figure 4
A photoacoustic endoscope with a miniaturized imaging probe

3.4. Photoacoustic tomography integrated with other imaging modalities

Combining complementary contrasts can potentially improve diagnostic accuracy. Because of its excellent optical absorption contrast, PAT has been integrated into various imaging modalities, such as ultrasound (US) imaging (mechanical contrast) [Fig. 5(A)] (17,37,38), OCT (optical scattering contrast) [Fig. 5(B)] (3941), confocal microscopy (scattering/fluorescence contrast) (4244), two-photon microscopy (fluorescence contrast) (45), and MRI (magnetic contrast) (46). Different modalities in hybrid systems usually share the same imaging area, thus their images are inherently co-registered.

Figure 5
Hybrid systems combining PAT with other imaging modalities

4. Anatomical and functional photoacoustic tomography using intrinsic contrasts

Theoretically, any intrinsic chromophore that has an optical absorption signature can potentially provide PAT contrast, as long as appropriate irradiation wavelengths are applied and the system sensitivity is sufficient. Here, we review the currently used intrinsic contrasts, in the order of hemoglobin, melanin, water, lipid, and nucleic acid.

4.1. Photoacoustic tomography of hemoglobin

In the visible spectral range (450–600 nm), oxyhemoglobin (HbO2) and deoxyhemoglobin (HbR) account for most of the optical absorption in blood (47). The absorption coefficient ratio between blood and surrounding tissues is as high as six orders of magnitude; hence, PAT can image with nearly no background RBC-perfused vasculature, the functional vascular subset responsible for tissue oxygen supply. Furthermore, because PA signal amplitudes depend on the concentrations of HbO2 (Cox) and HbR (Cde), spectroscopic measurements can be performed to quantify Cox and Cde by solving linear equations (48). From Cox and Cde, the total hemoglobin concentration (HbT) and oxygen saturation of hemoglobin (sO2) can be derived. Alternatively, HbT and sO2 can also be recovered by analyzing the acoustic spectrum (49).

4.1.1. Whole-body photoacoustic tomography of small animals

Small animals, especially mice, are extensively used in preclinical research on human diseases (50,51). Non-invasive whole-body imaging of small animals with high spatial resolution is extremely desirable for systemic studies of such as tumor metastases (52), drug delivery (53), and embryonic development (54).

Laufer et al. have recently reported whole-body images of the vasculature of transgenic mouse embryos, using Fabry-Perot interferometer (FPI)-based PACT [Fig. 6(A)] (30). The vasculature of the head, heart, and spinal cord is clearly visible. This work may enable longitudinal studies of the effects of genetic knockouts on the development of vascular malformations. Brecht et al. reported the first in vivo whole-body PAT images of a mouse [Fig. 6(B)] (23). The 3D tomography clearly shows blood-rich internal organs such as the liver, spleen, and kidneys, as well as large and small vasculature. Buehler et al. developed a novel PAT scanner capable of fast whole-body imaging in vivo (55). The system has achieved cross-sectional animal imaging with video-rate data acquisition. Imaging performance was demonstrated by resolving the mouse kidney anatomy, which was congruent with the corresponding histological results [Figs. 6(C–E)].

Figure 6
Whole-body photoacoustic tomography

4.1.2. Photoacoustic tomography of human breast

As the leading cause of cancer death among women, breast cancer can be diagnosed earlier by periodic screening (56). Currently, X-ray mammography is the only tool used for mass screening, and it has helped to increase the survival rate of breast cancer patients (57). However, in addition to the accumulation of ionizing radiation dose during lifetime screening, mammography also suffers from low sensitivity for early stage tumors in young women (58). To solve these problems, non-ionizing-radiation based techniques have been investigated, such as ultrasound, MRI, and PAT (24,59,60). Among these techniques, PAT is superior in contrast, sensitivity, and cost effectiveness. The PAT contrast is contributed by the angiogenesis-associated microvasculature around and within the tumor.

Ermilov et al. have used PAT to image breast cancer in humans (60). They imaged single breast slices in craniocaudal or mediolateral projection with at least 0.5 mm resolution. Fig. 7 shows an example of the superior contrast of PAT breast cancer imaging over X-ray mammography on a radiologically dense breast. A poorly differentiated infiltrating carcinoma can hardly be localized in the mammography image, but it can be easily visualized in the PAT image, with well-defined boundaries. Statistically, preliminary clinical studies demonstrated that PAT was able to visualize 18 malignant tumors out of 20 detected by biopsy, while X-ray mammography only detected 14 o them.

Figure 7
Photoacoustic tomography of human breast cancer

4.1.3. High-resolution functional photoacoustic tomography of microvasculature

Microvasculature, the distal portion of the cardiovascular system, delivers oxygen, humoral agents, and nutrients to the surrounding tissue and collects metabolic waste (61). Almost any microvasculature-associated parameter has important pathophysiological indications. PAT is highly desirable for microvasculature imaging because of its high spatial resolution and endogenous hemoglobin absorption contrast (11). Three representative applications are introduced here.

Non-invasive, high-resolution PAT of mouse brain activity may help to understand the human neurological diseases. Mouse cortical vasculature and vessel-by-vessel sO2 mapping obtained by OR-PAM are shown in Figs. 8(A) and (B), respectively (9,62). Major vascular landmarks can be well identified. The strong capability of PAT for functional brain imaging will greatly advance neurological studies.

Figure 8
Photoacoustic tomography of microvasculature

Many eye diseases are associated with altered eye microvasculature. So far, PAT has been demonstrated to be safe for ocular and retinal microvasculature imaging in small animals [Figs. 8(C–D)] (39,6366). All the major vascular components can be clearly visualized under the ANSI safety standard for the eye. sO2 in the iris microvasculature is also imaged spectrally. PAT offers significant promise for radiation-free monitoring of eye diseases.

Anti-angiogenesis is an important cancer treatment strategy (67). PAT is an ideal tool for angiogenesis-associated studies and has been applied to various tumor models, such as melanoma, glioblastoma, adenocarcinoma, carcinoma, and gliosarcoma (8). Fig. 8(E) shows an implanted human colorectal adenocarcinoma LS174T imaged by FPI-PACT (31). The image reveals a poorly and heterogeneously vascularized tumor core supplied by larger vessels around its periphery, which is a known feature of this type of tumor. PAT characterization of tumor vasculature will aid the development and refinement of new cancer therapies.

4.2. Photoacoustic tomography of melanin

Although it is the foremost killer among skin cancers, melanoma can be cured if detected early (68). PAT has been investigated for non-invasive melanoma imaging using melanin, the light-absorbing molecules in melanosomes, as the contrast (12,15,69,70). The absorption of melanin is ~1000 times that of water at 700 nm, which can potentially enable PAT to detect early melanoma in deep tissue.

Fig. 9(A) shows the blood vessels (in red) and melanoma (in brown) in the ear of a nude mouse imaged by OR-PAM (15). The melanoma generated stronger PA signals than the vessels and can be easily identified from the blood vessels by taking the difference before and after the injection of tumor cells. Because of the high scalability of PAT, the spatial resolution can be scaled down to sub-micrometer for single melanoma cell imaging by using an optical objective with higher NA [Fig. 9(B)] (15), or scaled up to sub-millimeter for brain melanoma growth monitoring with intact skull and scalp by changing to acoustic resolution [Fig. 9(C)] (69).

Figure 9
Photoacoustic tomography of melanin

4.3. Photoacoustic tomography of water

Water is the most abundant chemical in human body (57% of the body weight) (71). The body water content can reflect a disease state. Because water has much stronger absorption than other tissue components in the spectral range between 920 to 1040 nm (72), PAT is a promising tool to provide high resolution water imaging with high sensitivity. Fig. 10 shows the first PAT water imaging in a tissue phantom (73). First, spectral measurements of water-ethanol mixtures demonstrate that water concentration can be resolved by multi-wavelength excitation [Fig. 10(A)]. Second, a tissue phantom (2% agar embedded in fat) experiment at 975 nm shows that PAT can be used for water detection at low concentration, such as in fat [Figs. 10(B–C)]. In vivo PAT water imaging is expected in the near future.

Figure 10
Photoacoustic tomography of water

4.4. Photoacoustic tomography of lipid

Cardiovascular disease (CVD) has been the number one cause of death in the United States for over a century. The majority of CVD is due to atherosclerosis, characterized by plaques building up inside the arterial wall (74). Lipid is a common constituent in atherosclerotic plaques, the location and area of which are closely related to the progression of the disease. PAT is well suited for lipid imaging: compared with water-based tissue components, lipid has a distinct absorption spectrum between 1150 nm and 1250 nm [Fig. 11(A)] (7577). A recent advance in PAT lipid imaging was reported by Allen et al. (77). A human aorta containing a raised lipid-rich plaque [Fig. 11(B)] was imaged at 1200 nm [Fig. 11(C)]. The plaque is clearly identified due to the strong absorption by lipid. The results demonstrate that spectroscopic PAT is a promising tool for lipid detection in atherosclerosis.

Figure 11
Photoacoustic tomography of lipid

4.5. Photoacoustic tomography of cell nuclei

Cell nuclei are organelles where major cell activities take place. Compared with those of normal cells, nuclei of cancer cells have folded shapes and enlarged size (78). Imaging cell nuclei plays a critical role in cancer diagnosis. Traditional imaging of cell nuclei needs tissue sectioning and histological staining, which are not applicable for in vivo studies. Because nucleic acids, the major components of DNA and RNA in cell nuclei, have strong absorption in the ultraviolet range (79), PAT is a good choice for imaging of cell nuclei using nucleic acids as intrinsic contrast.

By exciting DNA and RNA at 266 nm, Yao et al. have recently reported the first label-free PA ex vivo and in vivo images of cell nuclei (Fig. 12) (80), termed UV-PAM. Cell nuclei in the epithelia of the mouse lip and the intestinal villi were imaged ex vivo [Figs. 12(A–B)]. Cell nuclei in the ear skin of a nude mouse were imaged in vivo at depths greater than 100 μm [Fig. 12(C)]. UV-PAM is cable of 3D noninvasive cell nuclei imaging without staining.

Figure 12
Photoacoustic tomography of cell nuclei

5. Chemical and molecular photoacoustic tomography using exogenous contrast agents

Even though the intrinsic contrasts in biological tissue are promising, exogenous contrast agents can extend the power of PAT. So far, optically absorptive organic dyes, nanoparticles, reporter genes (81), fluorescent proteins, microbubbles, and nanobubbles (82) have been successfully applied to PAT imaging. A thorough discussion of these agents can be found in a recent Review article (8), and only a few new applications are presented here.

Organic dyes, such as indocyanine green (ICG), IRDye800-NHS, methylene blue (MB), Evans blue (EB), and Congo red are widely used in PAT applications, including brain cortical structure enhancement (83), kidney perfusion (55), brain hemodynamic monitoring (20), tumor targeting (84), sentinel lymph node (SLN) mapping (37,38,85), capillary enhancement (86), and amyloid plaque staining (87). These dyes usually have peak absorption wavelengths within the near-infrared range, where blood and water have weak absorption; hence, the penetration depth can be improved. Fig. 13 shows an example of SLN mapping using MB as the contrast (38). An imaging depth of greater than 2 cm was demonstrated. This study is an important step towards the clinical translation of PAT. In addition to increasing the penetration depth, when conjugated with other functional ligands, organic dyes can specifically target cellular sites of interest, such as tumor cell membranes.

Figure 13
Photoacoustic tomography of sentinel lymph node (SLN) using methylene blue (MB)

Nanoparticles are of great research interest in PAT applications, and they have proven effective in delivering therapeutic agents by targeting specific sites (88,89). Because the properties of nanoparticles are highly size-dependent, their absorption spectra can be optimized by adjusting the particle geometry and dimensions, which makes them more flexible than organic dyes (8). In addition, targeted nanoparticles can significantly improve the imaging specificity of PAT (90). So far, different kinds of nanoparticles, including nanocages, nanoshells, nanorods, nanotubes, nanobeacons, and nanowontons, have been explored as PAT contrasts for different applications, such as cerebral cortex imaging (91), SLN mapping (92), macrophage imaging in atherosclerosis (93), and solid tumor targeting (94). Nanoparticle enhanced PAT has become a hot topic in biomedical studies.

As fluorescent proteins have totally redefined the ways in which biologists investigate the cellular and subcellular progress, deep fluorescent protein imaging by PAT has extended the ways we use these proteins (4,81,95). The multispectral PAT technique is capable of detecting fluorescent proteins within highly light-scattering organisms (81,96). Razansky et al. showed the feasibility of resolving tissue-specific expression of mCherry proteins in vivo (25). As shown in Fig. 14, whole-body spectroscopic PA imaging was performed on an adult zebra fish. The location of mCherry expression was accurately resolved [Fig. 14(D); red corresponds to the mCherry-expressing vertebral column]. The PAT image resolution is better than 40 μm at depth >1 mm, while confocal microscopy can hardly penetrate 500 μm [Fig. 14(G)].

Figure 14
Photoacoustic tomography of deep-seated fluorescent protein mCherry of a zebrafish in vivo

6. Photoacoustic tomography of fluid dynamics

Flow, an important contrast for biomedical imaging, provides much useful pathophysiological information. PAT is receiving increased attention as a tool to measure flow, as in PAT flowmetry. PAT flowmetry keeps all the merits of PAT and can perform better than scattering-based optical flowmetries in deep tissue.

So far, several principles have been proposed for PA measurement of flow. Fang et al. discovered the photoacoustic Doppler effect (PAD), and used an intensity-modulated CW laser to measure the flow speed based on the PAD frequency shift (97). Sheinfeld et al. extended this method by using pulsed sinusoidal (burst) excitation to attain axial information (98). Brunker et al. quantified the Doppler time shifts via cross-correlation of pairs of photoacoustic waveforms to measure the flow speed (99). Wei et al. extracted flow information based on rod-to-sphere shape transformations of gold nanorods induced by pulsed-laser irradiation (100). Fang et al. invented M-mode particle flowmetry by measuring the traveling time of absorptive particles across the optical illumination area (101). Yao et al. have reported a transverse flow imaging strategy on the basis of Doppler bandwidth broadening [Fig. 15(A)] (102,103). Using this method, the blood flow of an artery-vein pair in a mouse ear was imaged [Fig. 15(B)]. Chen et al. demonstrated a similar idea but conducted the analysis via auto-correlation in the time domain (104).

Figure 15
Photoacoustic tomography of blood flow using Doppler broadening of bandwidth

7. Prospects and summary

From organelles to whole bodies, from superficial microvasculature to internal organs, from anatomy to functions, PAT is playing an increasingly important role in basic physiological research and pre-clinical studies. Exciting events are happening in this fast growing field. Several prospects and corresponding challenges are discussed here.

Integration of the state-of-the-art techniques in system implementation will eventually lead PAT to commercialization for clinical practice. For PAM, a fast scanning mechanism and a high repetition rate laser with a wide tuning range of wavelengths are necessary for real-time functional imaging without compromising the spatial resolution. Also, a better optical and acoustic focusing method is needed to maintain the resolution in the depth dimension. For PACT, new techniques for assembling ultrasound arrays help to increase the imaging sensitivity and improve the spatial resolution (105). More homogeneous beam expansion over the sample surface is necessary for whole-field illumination. Parallel, real-time data acquisition is also important for further improving the imaging speed. For PAE, time gating may be necessary for eliminating motion artifacts induced by heart beating and breathing. Moreover, element minimization can still be improved to reduce the probe size to less than 1 mm in diameter. For PAT systems integrated with other modalities, multimodal contrast agents show great promise in providing complementary information (106).

Robust, fast, and automatic data processing will greatly enhance PAT performance. Currently, different inverse reconstruction methods have been used to improve PAT image quality, such as compressed-sensing (107), deconvolution (108), and wavelet filtering (109) based algorithms. However, each method usually works well only under some specific conditions, and the 3D reconstruction speed is usually too slow for clinical usage. In addition, the heterogeneity of optical fluence and sound speed distribution within the imaged plane may also degrade the reconstructed image. Therefore, a fast, quantitative, universally applicable image reconstruction algorithm is desirable. Besides image reconstruction, pre-processing of raw data and postprocessing of reconstructed images are also important. The former can help image reconstruction by de-noising and data compressing, while the latter can aid physicians in analyzing the results and thus making better diagnosis.

New imaging principles will be explored using PAT. In the PA effect, as irradiation intensity increases, mechanisms such as saturation of the optical absorption or multiphoton/multistep absorption can occur, resulting in a nonlinear dependence of the photoacoustic signal on the excitation pulse fluence (110,111). Danielli et al. recently reported a relaxation photoacoustic microscopy (rPAM) (111). From the saturation of the optical absorption, picosecond relaxation times of different chromophores were measured using nanosecond laser pulses. Nonlinear photoacoustic tomography will potentially become an interesting direction.

New parameters can be measured by PAT. For example, by combining the oxyhemoglobin concentration and volumetric blood flow, the metabolic rate of oxygen can be computed in the region of interest (6). Moreover, if the blood glucose concentration is also measured by PAT, aerobic and anaerobic metabolism of glucose can be quantified, respectively, which will be extremely useful for cancer diagnosis and treatment evaluation.

In summary, photoacoustic tomography perfectly complements other biomedical imaging modalities by providing unique optical absorption contrast with highly scalable spatial resolution, penetration depth, and imaging speed. In light of its capabilities and flexibilities, PAT is expected to play a more essential role in biomedical studies and clinical practice.

Acknowledgments

The authors thank Prof. James Ballard for manuscript editing. This research was supported by the National Institutes of Health Grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 EB010049, and 5P60 DK02057933. L.V.W. has financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.

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