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Copyright © 2011 Shi et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
TABLE 1:
Canavanine-resistant phenotypes of recycling mutants.
StrainScoreStrainScoreStrainScore
Wild type−snx3Δ vps5Δ++snx41Δ snx3Δ++++
snx4Δ++snx4Δ snx41Δ snx4Δ++snx4Δ snx3Δ++++
snx41Δ++vps5Δ ere1Δ++snx4Δ vps35Δ++++
snx42Δ+snx3Δ ere1Δ++snx4Δ ere1Δ++++
snx3Δ++snx3Δ ere2Δ++snx41Δ ere1Δ++++
vps5Δ++ere1Δ ere2Δ++snx41Δ ere2Δ++++
vps17Δ++apl2Δ−
vps26Δ+gga2Δ−
vps29Δ++ent3Δ−
vps35Δ++ent5Δ−
ere1Δ++
ere2Δ++
The Ere1 and Ere2 proteins function in the retromer recycling pathway. Serial dilutions (fourfold) of wild-type and different mutant cells (as indicated) were grown on synthetic media plates with or without 2.0 μg/ml canavanine for 2 d at 26°C. The canavanine-resistant phenotype of each strain was scored based on the relative growth of serial dilutions on canavanine plates. Relative growth: no growth at 0.1 OD600/ml was scored as −; growth at 0.1 OD600/ml was scored as +; growth at 2.5 × 10−2 OD600/ml was scored as ++; growth at 1.56 × 10−3 OD600/ml was scored as ++++.
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