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TABLE 1:

Canavanine-resistant phenotypes of recycling mutants.

StrainScoreStrainScoreStrainScore
Wild typesnx3Δ vps5Δ++snx41Δ snx3Δ++++
snx4Δ++snx4Δ snx41Δ snx4Δ++snx4Δ snx3Δ++++
snx41Δ++vps5Δ ere1Δ++snx4Δ vps35Δ++++
snx42Δ+snx3Δ ere1Δ++snx4Δ ere1Δ++++
snx3Δ++snx3Δ ere2Δ++snx41Δ ere1Δ++++
vps5Δ++ere1Δ ere2Δ++snx41Δ ere2Δ++++
vps17Δ++apl2Δ
vps26Δ+gga2Δ
vps29Δ++ent3Δ
vps35Δ++ent5Δ
ere1Δ++
ere2Δ++

The Ere1 and Ere2 proteins function in the retromer recycling pathway. Serial dilutions (fourfold) of wild-type and different mutant cells (as indicated) were grown on synthetic media plates with or without 2.0 μg/ml canavanine for 2 d at 26°C. The canavanine-resistant phenotype of each strain was scored based on the relative growth of serial dilutions on canavanine plates. Relative growth: no growth at 0.1 OD600/ml was scored as −; growth at 0.1 OD600/ml was scored as +; growth at 2.5 × 10−2 OD600/ml was scored as ++; growth at 1.56 × 10−3 OD600/ml was scored as ++++.