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|Wild type||−||snx3Δ vps5Δ||++||snx41Δ snx3Δ||++++|
|snx4Δ||++||snx4Δ snx41Δ snx4Δ||++||snx4Δ snx3Δ||++++|
|snx41Δ||++||vps5Δ ere1Δ||++||snx4Δ vps35Δ||++++|
|snx42Δ||+||snx3Δ ere1Δ||++||snx4Δ ere1Δ||++++|
|snx3Δ||++||snx3Δ ere2Δ||++||snx41Δ ere1Δ||++++|
|vps5Δ||++||ere1Δ ere2Δ||++||snx41Δ ere2Δ||++++|
The Ere1 and Ere2 proteins function in the retromer recycling pathway. Serial dilutions (fourfold) of wild-type and different mutant cells (as indicated) were grown on synthetic media plates with or without 2.0 μg/ml canavanine for 2 d at 26°C. The canavanine-resistant phenotype of each strain was scored based on the relative growth of serial dilutions on canavanine plates. Relative growth: no growth at 0.1 OD600/ml was scored as −; growth at 0.1 OD600/ml was scored as +; growth at 2.5 × 10−2 OD600/ml was scored as ++; growth at 1.56 × 10−3 OD600/ml was scored as ++++.