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Tr-fluorescence decay plots for IN–viral DNA and IN–LEDGF/p75 complexes. (A) IN alone; (B) IN–LEDGF/p75 complex, (C) IN–LEDGF/p75 complex was preformed and then exposed to viral DNA; (D) IN–viral DNA complex; (E) IN–viral DNA complex was preformed and then LEDGF/p75 was added to the nucleoprotein complex. Blue plots show fluorescence decays for donor only control, where Alexa 488-labeled IN(C56/65S) was mixed with the unlabeled protein. Magenta plots show data for the D–A pairs.