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Am J Epidemiol. Sep 1, 2011; 174(5): 528–536.
Published online Jun 27, 2011. doi:  10.1093/aje/kwr120
PMCID: PMC3202150
Association of Small Artery Elasticity With Incident Cardiovascular Disease in Older Adults
The Multi-Ethnic Study of Atherosclerosis
Daniel A. Duprez,* David R. Jacobs, Jr., Pamela L. Lutsey, David A. Bluemke, Lyndia C. Brumback, Joseph F. Polak, Carmen A. Peralta, Philip Greenland, and Richard A. Kronmal
*Correspondence to Dr. Daniel A. Duprez, Cardiovascular Division, Medical School, University of Minnesota, VCRC–Room 270, MMC 508, 420 Delaware Street SE, Minneapolis, MN 55455 (e-mail: dupre007/at/umn.edu).
Received June 1, 2010; Accepted March 17, 2011.
Functional biomarkers like large artery elasticity (LAE) and small artery elasticity (SAE) may predict cardiovascular disease (CVD) events beyond blood pressure. The authors examined the prognostic value of LAE and SAE for clinical CVD events among 6,235 Multi-Ethnic Study of Atherosclerosis participants who were initially aged 45–84 years and without symptomatic CVD. LAE and SAE were derived from diastolic pulse contour analysis. During a median 5.8 years of follow-up between 2000 and 2008, 454 adjudicated CVD events occurred, including 256 cases of coronary heart disease (CHD), 93 strokes, and 126 heart failures (multiple diagnoses were possible). After adjustment for age, race/ethnicity, sex, clinic, height, heart rate, body mass index, systolic and diastolic blood pressure, use of antihypertensive and cholesterol-lowering medications, smoking, total cholesterol, high density lipoprotein cholesterol, triglycerides, diabetes, and high-sensitivity C-reactive protein, the hazard ratio for any CVD per standard-deviation increase in SAE was 0.71 (95% confidence interval: 0.61, 0.83; P < 0.0001). The lowest (stiffest) SAE quartile had a hazard ratio of 2.28 (95% confidence interval: 1.55, 3.36) versus the highest (most elastic) quartile. The net reclassification index, conditional on base risk, was 0.11. SAE was significantly associated with future CHD, stroke, and heart failure. After adjustment, LAE was not significantly related to CVD. In asymptomatic participants free of overt CVD, lower SAE added prognostic information for CVD, CHD, stroke, and heart failure events.
Keywords: arteries, cardiovascular diseases, elasticity, risk factors
Early risk identification can help prevent clinical cardiovascular disease (CVD). Persons at risk for CVD events are currently identified by screening for cholesterol, glucose, smoking, obesity, and blood pressure (1, 2). By identifying asymptomatic persons with evidence of early CVD, investigators can delineate a subpopulation that is likely to progress to clinical events, beyond prediction possible from risk factors alone (3).
Several structural markers for vascular disease, such as carotid intima-media thickness and coronary calcium score (47), have been studied in relation to CVD risk factors and coronary heart disease (CHD) events. In contrast, the functional vascular marker arterial stiffness probably captures an earlier phase of CVD development. Measures of arterial stiffness have been linked with CVD events (812). Carotid-femoral pulse wave velocity is the measure of arterial stiffness most studied in relation to CHD and stroke events. An alternative method derives small arterial elasticity (SAE) from the diastolic pulse contour analysis obtained from the radial artery waveform. In a limited retrospective study by Grey et al. (13), an association was shown between lower SAE and self-reported CVD events. Aortic stiffness has also been associated with incident hypertension (14, 15). In the Multi-Ethnic Study of Atherosclerosis (MESA), the finding was that lower SAE was the strongest predictor of incident hypertension out of several arterial measures, including measures of large arterial stiffness and aortic distensibility (16). The small arteries, which are the primary source of the oscillatory compliance of the vascular tree, may be uniquely important in the development and initiation of hypertension (16), relative to the vascular stiffness and atherosclerotic plaque deposition of the larger vessels. Similar mechanisms may be involved in development of CVD.
We hypothesized that in MESA, which contained a large sample of persons who were free of overt CVD, SAE would predict CVD events and that large artery elasticity (LAE), following the pattern observed for incident hypertension (16), would be a more limited predictor.
Study sample
MESA was initiated to investigate the prevalence, correlates, and progression of subclinical (asymptomatic) CVD in men and women (17). In brief, between July 2000 and August 2002, 6,814 men and women who identified themselves as white, black, Hispanic, or Chinese, were aged 45–84 years, and were free of clinically apparent CVD were recruited from portions of 6 US communities: Baltimore, Maryland; Chicago, Illinois; Forsyth County, North Carolina; Los Angeles County, California; northern Manhattan and the Bronx, New York, New York; and St. Paul, Minnesota. Investigators at each clinic recruited from locally available sources, which included lists of residents, dwellings, and telephone exchanges. In the last few months of the recruitment period, supplemental sources (Centers for Medicare and Medicaid Services lists and referrals by participants) were used to ensure adequate numbers of minorities and elderly subjects. The institutional review boards at all participating centers approved the study, and all participants gave informed consent.
Data collection and definitions of risk factors
Age, race/ethnicity, and sex were self-reported during the recruitment phase and verified at baseline. Height and weight were measured with participants wearing light clothing and no shoes. Body mass index was calculated as weight (kg) divided by height squared (m2). Resting seated blood pressure was measured 3 times with a Dinamap model Pro 100 automated oscillometric sphygmomanometer (Critikon, Tampa, Florida). The average of the last 2 measurements was used. Hypertension was defined as systolic blood pressure ≥140 mm Hg, diastolic blood pressure ≥90 mm Hg, or current use of antihypertensive medication. Standardized questionnaires were used, and medication containers were examined. The participants were classified as current cigarette smokers, former smokers, or never smokers. Diabetes was defined as a fasting plasma glucose level ≥126 mg/dL (≥7 mmol/L) or a history of medical treatment for diabetes; almost all persons with diabetes had type 2 diabetes.
Blood measures
A central laboratory (University of Vermont, Burlington, Vermont) measured levels of total and high density lipoprotein cholesterol, triglycerides, plasma glucose, and high-sensitivity C-reactive protein in blood samples obtained after a 12-hour fast.
LAE and SAE measurements
Arterial wave forms were recorded using the HDI/PulseWave CR-2000 (Hypertension Diagnostics, Inc., Eagan, Minnesota). Of 6,814 MESA participants, the arterial elasticity measure was unobtainable in 2.7% (n = 184) because of refusal (n = 10), physical inability (n = 48), equipment malfunction (n = 41), clinic time constraints with a failed attempt to reschedule another visit (n = 39), or other, unknown reasons (n = 46). Of the 6,630 persons in whom the procedure was carried out, LAE and SAE could not be estimated in 294 (4.4%) because of arrhythmia or participant movement. LAE and SAE were estimated in 6,336 participants, of whom 7 were found after baseline to have preexisting CVD and were excluded from these analyses. Thus, LAE and SAE were obtained in 93% of those participants in whom the procedure was attempted. After adjustment for age, race/ethnicity, and sex, no other risk factor or subclinical measure differed between the 478 persons who were not measured and the 6,336 who were measured.
A solid-state pressure transducer array (tonometer) was placed over the radial artery of the dominant arm to record the pulse contour. A 30-second analog tracing of the radial waveform, constituting continuous pressure changes during diastole, was digitized at 200 samples per second. There was an accompanying automated, oscillatory blood pressure measurement at the brachial artery of the contralateral arm.
SAE and LAE are estimated by the device (18) from the waveform modeled as a decaying exponential function plus a sinusoidal function dampened by a decaying exponential (see Web Appendix (http://aje.oxfordjournals.org/)). The quantities estimated directly from the waveform are X and Y. LAE and SAE are estimated by dividing each of X and Y by systemic vascular resistance (SVR). SVR is estimated as mean arterial blood pressure/cardiac output, and cardiac output is estimated from ejection time taken from the pulse waveform, heart rate, age, height, and weight (see Web Appendix).
The same MESA technician performed a repeat measure on the same day during the baseline clinic using the CR-2000 in 131 people (19). The mean difference was −0.13 mL/mm Hg (standard deviation [SD], 3.5) × 10 for LAE, −0.03 mL/mm Hg (SD, 1.5) × 100 for SAE, 0.01 seconds (SD, 4.6) for LAE × SVR, 0.005 seconds (SD, 1.8) × 10 for SAE × SVR, 2.8 mm Hg (SD, 8.2) for systolic blood pressure, and 1.5 mm Hg (SD, 5.0) for diastolic blood pressure. The between-measure correlation was 0.74 for LAE, 0.84 for SAE, 0.58 for LAE × SVR, 0.74 for SAE × SVR, 0.90 for systolic blood pressure, and 0.86 for diastolic blood pressure. Reproducibility findings did not differ significantly between centers.
Follow-up
We recorded new symptomatic and adjudicated cardiovascular events for a median of 5.8 years between July 2000 and August 2008 (10th, 25th, 75th, and 90th percentiles of follow-up time: 3.6, 5.6, 5.9, and 6.1 years, respectively), through the end of 7 follow-up contacts at intervals of 9–12 months. At each contact, an interviewer contacted each participant or a family member by telephone to inquire about interim hospital admissions, outpatient diagnoses of CVD, and deaths. To verify self-reported diagnoses, we requested copies of medical records for participants who had been hospitalized or had received an outpatient diagnosis of CVD. We obtained records of 98% of reported cardiovascular events associated with hospitalization. For participants who had died of cardiovascular causes outside the hospital, we conducted interviews with the next of kin and requested copies of death certificates. Physicians adjudicated the diagnoses; only symptomatic events were counted. The outcome “CVD event” comprised myocardial infarction, death from CHD, angina, heart failure, stroke, and peripheral vascular disease, while “CHD event” included myocardial infarction, death from CHD, and angina. Details are given in the Web Appendix and at www.mesa-nhlbi.org.
Statistical analysis
We restricted the data to 454 CVD events in 6,235 participants who had no information missing for outcome, arterial elasticity, or covariables. We used Cox proportional hazards regression to estimate hazard ratios for a CVD event, a CHD event, and each of the specific entities (myocardial infarction or death from CHD, angina, heart failure, stroke, and peripheral vascular disease) according to SAE as a continuous variable divided by its standard deviation, and also as sex-specific quartiles. Model 1 (minimally adjusted) included the covariates age, sex, race/ethnicity, clinic, and height. Model 2 (fully adjusted) added the covariates heart rate, systolic and diastolic blood pressures, use of antihypertensive medication, body mass index, smoking (3 categories), total cholesterol, high density lipoprotein cholesterol, triglycerides, use of cholesterol-lowering medication, diabetes, and high-sensitivity C-reactive protein. Adding educational attainment to the model did not substantially alter findings (data not shown). These analyses were repeated using LAE as the predictor of interest.
C statistics (area under the receiver operating characteristic curve) were approximated by means of corresponding logistic models (odds ratios in the logistic models approximated those in the proportional hazards models). The net reclassification index (NRI), after inclusion of SAE in model 2 (base risk model without SAE), was computed as [proportion of all CVD events reclassified at higher risk – proportion reclassified at lower risk] – [proportion of all nonevents reclassified at higher risk – proportion reclassified at lower risk] (20). Unstratified NRI is confounded with base risk (in this paper, without SAE in the model) whenever the base risk function is related to actual risk, but the question we are asking is whether SAE improves classification with knowledge of base risk; therefore, in this paper we report the NRI within each quartile of this base risk. Recognizing that the weighting of NRI values over quartiles may be debated, we also present the equiweighted average of the reclassification proportions across quartiles, reflecting equal numbers of persons at risk in each base risk quartile. NRI variances were computed using an exact formula (see tabular footnote), resulting in standard errors that were 5%–22% lower than the approximation used by Pencina et al. (20). In parallel models, we replaced SAE with SAE × SVR to examine information obtained solely from the pulse waveform (SAE × SVR) separately from that estimated from physical measures (SVR). All analyses were performed with SAS software, version 9.2 (SAS Institute Inc., Cary, North Carolina). We regarded P values less than 0.05 as statistically significant.
Table 1 shows sex-specific baseline characteristics and describes LAE and SAE, as previously reported (21). The age- and sex-adjusted correlation coefficient for correlation between LAE and SAE was 0.23. Most of the baseline characteristics varied significantly by sex.
Table 1.
Table 1.
Characteristics of Participants by Sex, Multi-Ethnic Study of Atherosclerosis, 2000–2002
With 454 incident CVD events, compared with the highest (most elastic) SAE quartile, the hazard ratio in the lowest (stiffest) SAE quartile in the fully adjusted model (model 2) was 2.28 (95% confidence interval: 1.55, 3.36), with graded risk in the intermediate quartiles (Table 2). The decrease in risk per standard-deviation increase in elasticity was 0.71 (P < 0.0001). Risk was similarly significantly elevated, generally in a graded fashion, in the presence of less elastic small arteries for each CVD entity, most clearly for stroke. The area under the receiver operating characteristic curve (c statistic) increased only modestly by 0.005 (from 0.777 to 0.782) with the addition of SAE to the variables otherwise included in model 2 for predicting CVD events (data not shown). A more informative measure was NRI within base risk quartile; it was 0.27, 0.05, 0.09, and 0.04 for quartiles 1–4, respectively (Table 3). Each of the stratum-specific NRIs, except the one derived in base risk quartile 2, were statistically significant. The equiweighted NRI was 0.11 and was highly statistically significant. Similar computations using age, race/ethnicity, sex, and Framingham 10-year CHD risk score as the base risk yielded an average NRI of 0.15 (Web Table 1).
Table 2.
Table 2.
Hazard Ratio for Cardiovascular Endpoints by Quartile of Small Artery Elasticity (n = 6,235), Multi-Ethnic Study of Atherosclerosis, 2000–2008
Table 3.
Table 3.
Net Reclassification Index for Predicted Incident Cardiovascular Disease Events With Inclusion of Small Artery Elasticity in the Prediction Model Versus Without Inclusion (454 Cases Among 6,235 Persons at Risk), Multi-Ethnic Study of Atherosclerosis, (more ...)
LAE showed prediction for several of the outcome variables in model 1 (Web Table 2) but retained statistical significance in model 2 only in prediction of heart failure (P = 0.03). Addition of SAE to the model for heart failure increased the P value for LAE to 0.06, while the corresponding P value for SAE (P = 0.01 in Table 2) increased to 0.02 (data not shown). The largest hazard ratio associated with LAE in Web Table 2 was 2.54, predicting incident stroke in the stiffest LAE quartile (quartile 1) versus the most elastic LAE quartile (quartile 4), but this hazard ratio was not significant. C statistics did not improve much by inclusion of LAE in the model, the largest improvement in model 2 being 0.002 for prediction of angina pectoris (data not shown).
We examined prediction of CVD events with the pulse waveform component of SAE, that is, SAE × SVR, which is a measure that indexes the amount and timing of oscillations in the diastolic waveform and does not include any physical measures in its estimation. The directionality and statistical significance of the findings (Web Table 3) were the same as in Table 2 (Web Table 4), except that the P value for heart failure in model 2 was 0.06 and the findings for SAE × SVR were weaker than those for SAE in prediction of stroke.
In sensitivity analyses, the sex interaction in prediction of CVD events from SAE was not statistically significant (P = 0.57). In separate models for women and men, among 170 CVD events in 3,265 women, the hazard ratio per standard-deviation increase in SAE was 0.72 (95% confidence interval: 0.52, 1.00), while in 2,970 men with 284 events, the corresponding hazard ratio was 0.70 (95% confidence interval: 0.58, 0.84).
This prospective analysis of the MESA cohort, whose participants were initially free of symptomatic CVD, showed that SAE provided incremental predictive information for any CVD event and for each of its different components: myocardial infarction and CHD death, angina, heart failure, stroke, and peripheral vascular disease. This predictive value was significant not only after adjustment for demographic and anthropometric characteristics but also with further adjustment for CVD risk factors. In contrast to SAE, LAE was also predictive of CVD events in models adjusted for demographic factors, but in the fully adjusted model (model 2) it retained statistical significance only for heart failure. The NRI, conditional on base risk, was 0.11. SAE and the part of SAE that is derived solely from the waveform, SAE × SVR, were similarly predictive of total CVD events.
Several noninvasive methods exist for assessing arterial stiffness/elasticity. The largest amount of evidence regarding arterial stiffness as an additive predictor beyond traditional risk factors for CVD events has been derived from carotid-femoral pulse wave velocity. Initial studies were mainly focused on subjects at high risk (end-stage renal disease, diabetes, hypertension) (22). Among the few studies of asymptomatic people, in the Rotterdam Study, a Dutch study of apparently healthy persons aged ≥55 years, aortic pulse wave velocity was associated with CHD and stroke (10). In a small group of 141 elderly persons (age range, 70–100 years), aortic pulse wave velocity was associated with cardiovascular death (11). In the Health ABC Study, which included people aged 70–79 years, aortic pulse wave velocity was associated with higher CVD mortality, CHD, and stroke, although the association was not as strong as might have been expected in the highest quartile of pulse wave velocity, and no association was found in predicting heart failure (8). In an asymptomatic Danish population aged 40–70 years, aortic pulse wave velocity showed a trend toward prediction of CHD after controlling for traditional risk factors (9). A smaller study of Japanese Americans living in Hawaii showed that aortic pulse wave velocity was associated with CVD death (12). In the Framingham Offspring Study (151 incident CVD events in 2,232 persons; average age = 63 years), the relative hazard was 1.48 per standard-deviation increase in aortic pulse wave velocity but was not significant for augmentation index (23). The above findings, plus findings in diseased persons, were summarized in a meta-analysis (24). A second meta-analysis (25) from the same group of investigators considered measures of central hemodynamics. The augmentation index was positively associated with CVD outcome and all-cause mortality in studies of diseased persons.
Interpretation of the 2 measures, LAE and SAE, as pertaining to elasticity of the large and small arteries is based on a literal interpretation of the windkessel (“air chamber”) model (18). An accessible heuristic description of the windkessel model is rapid drainage of the blood into a large sink (the larger arteries), with subsequent evacuation from the sink into a smaller container (the smaller arteries). There has been some skepticism in the literature regarding LAE and SAE (26). LAE and SAE are derived from an algebraic decomposition of the diastolic waveform into one part that is primarily declining between aortic closure and aortic opening (decaying exponential function) and a second part that is largely flat but may be oscillating (sinusoidal function dampened by a decaying exponential function) (18). An alternative to the windkessel model is to interpret SAE by considering the possible sources of the oscillations during diastole (second part of the algebraic decomposition), when the left ventricle is considered to be “still.” The oscillations could be the result of a variety of activities of the arterial system during diastole, including left ventricular filling and untwisting, accommodation of reflection waves, spontaneous contraction of the vascular smooth muscle cells, and slow dissipation of the blood over the precapillary sphincters.
The pulse wave velocity (derived from behavior of the large arteries), augmentation index (derived from the systolic waveform), LAE, and SAE (derived from the oscillatory component of the diastolic waveform) appear to impart some common information, given that they were correlated in several studies (2731). Observed correlations between SAE and augmentation index included −0.36 (27), −0.59 (28), and −0.71 (29), while correlations of LAE with augmentation index were −0.36 (28) and −0.41 (30). The correlation between aortic pulse wave velocity and augmentation index was 0.56 (30); that between SAE and femoral-popliteal pulse wave velocity was −0.52 in healthy subjects and −0.34 in older subjects with diabetes, while corresponding correlations with LAE were −0.32 and −0.46 (31). Concordance among pulse wave velocity, augmentation index, and SAE was further elucidated in the experimental study by Gilani et al. (32). They blocked nitric oxide release by NG-nitro-L-arginine methyl ester in 10 healthy persons; this blockade resulted in an increase in aortic pulse wave velocity (8.25–8.98 m/second; P = 0.04), an increase in augmentation index (48.3%–64.6%; P < 0.05), and a decrease in SAE (9.9–6.9 mL/mm Hg × 100; P < 0.001). LAE, in contrast, did not change significantly (32). However, there are also differences among these measures. This was illustrated in the Anglo-Cardiff Collaborative Trial, where a different age course was seen for augmentation index (increased rapidly during middle age and slowly in the elderly) than for pulse wave velocity (changed slowly during middle age and rapidly in the elderly) (30). Morbidity and mortality studies comparing and contrasting these 3 measures and other measures would be useful.
In a theory of a temporal sequence of events, vascular disease originates in endothelial dysfunction, which has a profound influence on the microvasculature. In this view, evaluation of the smaller arteries and other microvasculature would be helpful in predicting early clinical events, consistent with our study. Stiffening of the small arteries, whether as a consequence of endothelial dysfunction, vasoconstriction, or structural changes due to remodeling, alters the magnitude and timing of reflected waves. Loss of arterial elasticity strengthens the early reflected pressure wave; the consequent increased afterload may eventually result in heart failure, as we observed in this study. In line with this temporal sequence hypothesis, the relatively weak predictive ability of LAE may reflect several features of the MESA sample, including the absence of people with clinical heart disease, their relatively low blood pressure, and the lack of extended follow-up. We speculate that abnormalities in large arteries, such as those reflected in low LAE, will gain predictive power as some MESA participants develop clinical CVD.
The current study was strengthened by the large sample size, the community-based setting, the multiethnic sample, and standardized subclinical atherosclerosis assessments and risk factor measurements. Other strengths were the prospective design and the reliance on adjudicated symptomatic endpoints, which avoids detection bias related to less thoroughly investigated CVD events or to cross-sectional evaluation of subjects with known subclinical atherosclerosis. This report fills a need, called for in a consensus document on arterial stiffness, to obtain “evidence, in a longitudinal study, that systemic arterial stiffness or systemic arterial compliance has independent predictive value for CV events” (33, p. 2594). Limitations include the relatively small number of peripheral vascular disease events to date. Results could be different for long-term CVD prediction, especially as this population ages and specific clinical manifestations of CVD emerge. Caution should be exercised in interpreting P values in light of the multiple comparisons made; the statistical tests were correlated because a participant may have had more than 1 of the outcome diagnoses. In addition, the exact value of NRI is sensitive to categorization and cutpoints selected to represent the base and augmented risk functions.
In conclusion, the present observation in a population sample without clinically overt CVD or a history of CVD demonstrated that standardized and quality-controlled noninvasive measurement of the radial artery pulse contour over a few heart cycles was significantly associated with future CVD events above and beyond classic risk factors.
Supplementary Material
Web Appendix
Acknowledgments
Author affiliations: Cardiovascular Division, Medical School, University of Minnesota, Minneapolis, Minnesota (Daniel A. Duprez); Division of Epidemiology and Community Health, School of Public Health, University of Minnesota, Minneapolis, Minnesota (David R. Jacobs, Jr., Pamela L. Lutsey); Clinical Center, National Institutes of Health, Bethesda, Maryland (David A. Bluemke); Department of Biostatistics, School of Public Health, University of Washington, Seattle, Washington (Lyndia C. Brumback, Richard A. Kronmal); Department of Radiology, Tufts Medical Center, Boston, Massachusetts (Joseph F. Polak); Division of Nephrology, Department of Medicine, UCSF Medical Center, University of California, San Francisco, San Francisco, California (Carmen A. Peralta); Department of Preventive Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois (Philip Greenland); and Department of Nutrition, School of Medicine, University of Oslo, Oslo, Norway (David R. Jacobs, Jr.).
This research was supported by contracts N01-HC-95159 through N01-HC-95169 and grant R01-HL-098382 from the National Heart, Lung, and Blood Institute and by grant RR-024156 from the National Center for Research Resources, National Institutes of Health.
The information contained herein was derived in part from data provided by the Bureau of Vital Statistics, New York City Department of Health and Mental Hygiene.
The authors thank the investigators and staff of the Multi-Ethnic Study of Atherosclerosis (MESA) for their valuable contributions. A full list of participating MESA investigators and institutions can be found at http://www.mesa-nhlbi.org.
Conflict of interest: none declared.
Glossary
Abbreviations
CHDcoronary heart disease
CVDcardiovascular disease
LAElarge artery elasticity
MESAMulti-Ethnic Study of Atherosclerosis
NRInet reclassification index
SAEsmall artery elasticity
SDstandard deviation
SVRsystemic vascular resistance

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