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Nucleic Acids Res. 1984 June 11; 12(11): 4531–4538.
PMCID: PMC318856

Evolution of haptoglobin: comparison of complementary DNA encoding Hp alpha 1S and Hp alpha 2FS.

Abstract

Haptoglobin is a transport glycoprotein which removes free hemoglobin from the circulation of vertebrates. In human populations haptoglobin is polymorphic due to three alleles, Hp alpha 1F, Hp alpha 1S and Hp alpha 2. The Hp alpha 2 allele is roughly twice the length of the Hp alpha 1 alleles and is the product of a partial gene duplication possibly resulting from an unequal crossover event in a heterozygous genotype Hp alpha 1F/Hp alpha 1S. In the study described here we compare the cDNA encoding Hp alpha 1S to that encoding Hp alpha 2FS . Both have a leader sequence followed by the genotypic alpha chain sequence, a beta sequence and an untranslated sequence in the 3' end. The cDNA encoding Hp alpha 2FS is composed of alpha 1F and alpha 1S domains differing by four nucleotide replacements. Hp alpha 1S cDNA contains the same replacement site mutations found in the alpha 1S domain of Hp alpha 2FS , indicating that this coding region has sustained few, if any, mutations since its incorporation into the Hp alpha 2FS gene.

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Selected References

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  • Yang F, Brune JL, Baldwin WD, Barnett DR, Bowman BH. Identification and characterization of human haptoglobin cDNA. Proc Natl Acad Sci U S A. 1983 Oct;80(19):5875–5879. [PubMed]
  • SMITHIES O, CONNELL GE, DIXON GH. Chromosomal rearrangements and the evolution of haptoglobin genes. Nature. 1962 Oct 20;196:232–236. [PubMed]
  • Barnett DR, Lee TH, Bowman BH. Amino-acid sequence of the carboxyl terminal octapeptide of human haptoglobin beta chain. Nature. 1970 Mar 7;225(5236):938–939. [PubMed]
  • Kurosky A, Barnett DR, Lee TH, Touchstone B, Hay RE, Arnott MS, Bowman BH, Fitch WM. Covalent structure of human haptoglobin: a serine protease homolog. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3388–3392. [PubMed]
  • Prochownik EV, Markham AF, Orkin SH. Isolation of a cDNA clone for human antithrombin III. J Biol Chem. 1983 Jul 10;258(13):8389–8394. [PubMed]
  • vander Straten A, Herzog A, Jacobs P, Cabezón T, Bollen A. Molecular cloning of human haptoglobin cDNA: evidence for a single mRNA coding for alpha 2 and beta chains. EMBO J. 1983;2(6):1003–1007. [PubMed]
  • Raugei G, Bensi G, Colantuoni V, Romano V, Santoro C, Costanzo F, Cortese R. Sequence of human haptoglobin cDNA: evidence that the alpha and beta subunits are coded by the same mRNA. Nucleic Acids Res. 1983 Sep 10;11(17):5811–5819. [PMC free article] [PubMed]
  • van der Straten A, Herzog A, Cabezón T, Bollen A. Characterization of human haptoglobin cDNAs coding for alpha 2FS beta and alpha 1S beta variants. FEBS Lett. 1984 Mar 12;168(1):103–107. [PubMed]
  • Sargent TD, Yang M, Bonner J. Nucleotide sequence of cloned rat serum albumin messenger RNA. Proc Natl Acad Sci U S A. 1981 Jan;78(1):243–246. [PubMed]
  • Takahashi N, Bauman RA, Ortel TL, Dwulet FE, Wang CC, Putnam FW. Internal triplication in the structure of human ceruloplasmin. Proc Natl Acad Sci U S A. 1983 Jan;80(1):115–119. [PubMed]
  • MacGillivray RT, Mendez E, Sinha SK, Sutton MR, Lineback-Zins J, Brew K. The complete amino acid sequence of human serum transferrin. Proc Natl Acad Sci U S A. 1982 Apr;79(8):2504–2508. [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press