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Nucleic Acids Res. 1984 June 11; 12(11): 4507–4516.
PMCID: PMC318854

A type II restriction endonuclease with an eight nucleotide specificity from Streptomyces fimbriatus.


A new site-specific endonuclease, Sfi I, has been isolated from Streptomyces fimbriatus . This is the first report of a type II restriction endonuclease whose recognition specificity requires eight nucleotides. Sfi I cleaves the sequence, GGCCNNNN / NGGCC , symmetrically to produce a three base, 3' extension.

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Selected References

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