Les expériences précoces de la vie modifient en permanence le comportement et la physiologie. Ces effets sont en partie, dus à des transformations prolongées de l'expression génique dans des régions cérébrales sélectionnées. La question principale concerne le mécanisme de ces effets «programmants» environnementaux. Nous examinons ce problème sur un modèle animal qui étudie les conséquences des variations des interactions mère-enfant sur le développement des différences individuelles dans les réponses au stress, endocrines et environnementales, à l'âge adulte. Un léchage/toilettage intense de petits de rats par leur mère dans la première semaine de vie modifie la structure de l'ADN au niveau du gène promoteur d'un récepteur glucocorticoïde dans l'hippocampe de la descendance. Les différences de schémas de méthylation de I'ADN entre les descendants de mères fortement ou faiblement lécheuses apparaissent après la première semaine de vie; elles sont réversibles avec l'échange des mères; elles persistent à l'âge adulte et sont associées à une modification de l'acétylation de l'histone et du facteur de transcription (facteur de croissance nerveux induit par clone A [NGFIA]) en liaison avec le promoteur du récepteur glucocorticoïde. La méthylation de I'ADN modifie l'expression du récepteur glucocorticoïde par les changements de structure de la chromatine. L'inversion pharmacologique induite par les changements de structure de la chromatine élimine complètement les effets des soins maternels sur l'expression du récepteur glucocorticoïde et les réponses au stress hypothalamo-adrénalo-pituitaires (HAP), suggérant donc une relation causale entre les modifications épigénétiques du gène du récepteur glucocorticoïde induites par la mère et les effets sur les réponses au stress dans la descendance. Ces résultats démontrent que les changements structuraux de l'ADN peuvent se constituer par l'intermédiaire d'un environnement programmant et que, malgré la stabilité inhérente de ce marqueur épigénomique, elles sont dynamiquement et potentiellement réversibles.

“Stress diathesis” models suggest that adversity in early life alters the development of neural and endocrine systems in a manner that predisposes individuals to disease in adulthood. The relation between the quality of the early environment and health in adulthood appears to be mediated by parental influences on the development of neural systems that underlie the expression of behavioral and endocrine responses to stress.^1,18-22 Adversity or decreased quality of parental investment increases the magnitude of emotional, autonomic, and hypothalamicpituitary-adrenal (HPA) responses to stress in adulthood. These models are constructed on two principal assumptions: (i) prolonged activation of neural and hormonal responses to stress can promote illness; (ii) early environmental events influence the development of these responses. There is strong evidence in favor of both ideas. In humans, forms of parenting that enhance the risk of chronic illness in the offspring increase endocrine and autonomic responses to stress in adulthood.^22-26 There is considerable evidence for comparable effects in primates^27-29 and rodents.^28,30 Moreover, prolonged exposure to elevated levels of stress hormones, including corticotropin-releasing factor (CRF), catecholamines (most notably norepinephrine), and glucocorticoids promote the development of a diverse range of high-risk conditions, such as visceral obesity, hypertension, and insulin intolerance, or overt pathology, including diabetes, depression, drug addiction, and multiple forms of coronary heart disease.^31-33 The clinical risks associated with prolonged activation of the HPA and autonomic systems are a logical consequence of the otherwise adaptive stress response. In response to neural signals associated with the stressor, there is an increased release of glucocorticoids from the adrenal gland and catecholamines, particularly norepinephrine from the sympathetic system. The combined actions of these hormones increase the availability of energy substrates, such as those derived from lipid and glucose metabolism, in order to maintain normal cellular output and organ efficiency. These actions protect against catastrophes such as hypotensive shock. These hormones, along with the central CRF and catecholamines, also act on multiple brain regions to increase vigilance and fear and enhance avoidance learning and fear conditioning, which reduces the chances of further encounters with the offending conditions. It is likely that such responses evolved to meet the demands of acute stressors, and that the physiological costs associated with short-term activation are minimal in otherwise healthy individuals. The high-risk conditions are associated with chronic stress and persistent activation of stress hormones.

Support for the basic elements of stress diathesis models appears compelling. Adversity during perinatal life alters development in a manner that seems likely to promote vulnerability, especially for stress-related diseases. Diathesis describes the interaction between development, including the potential influence of genetic factors, and the prevailing level of stress in predicting health outcomes. Such models have considerable appeal, and could potentially identify both the origins and the nature of vulnerability derived from either epigenetic influences, such as early family life, or genomic variations.^27,34 For developmentalists the critical questions are (i) how early experience might “program” individual differences in stress responses; and (ii) whether such effects are reversible.

We examine the relation between maternal care and the development of stress responses using a rather simple model of naturally occurring variations in maternal behavior over the first 8 days after birth.⁶⁵ We characterize individual differences in maternal behavior through direct observation of mother-pup interactions in normally reared animals. These observations reveal considerable variation in maternal LG of pups (Figure 1). LG includes both body as well as anogenital licking.⁶⁶ We then simply define mothers according to the frequency of pup LG, ie, high- or low-LG mothers. For the sake of most of the studies described here, high- and low-LG mothers are females whose scores on pup LG are ±1 SD above (high) or below (low) the mean for their cohort. Importantly, high- and low-LG mothers do not differ in the amount of contact time with pups; differences in the frequency of LG do not occur simply as a function of time in contact with pups. High- and low-LG mothers raise a comparable number of pups to weaning, and there are no differences in the weaning weights of the pups, suggesting an adequate level of maternal care across the groups. These findings also suggest that we are examining the consequences of variations in maternal care that occur within a normal range. Indeed, the frequency of both pup LG is normally distributed across large populations of lactating female rats.⁶⁵

The critical question concerns the potential consequences of these differences in maternal behavior for the development of behavioral and neuroendocrine responses to stress. As adults, the offspring of high-LG mothers show reduced plasma ACTH and corticosterone responses to acute stress by comparison to the adult offspring of lowLG mothers.^39,67 Circulating glucocorticoids act at GR sites in corticolimbic structures, such as the hippocampus, to regulate HPA activity. Such negative-feedback effects commonly target CRF synthesis and release at the level of the PVNh. Enhanced GR activation in the hippocampus is associated with decreased hypothalamic CRF levels. The high-LG offspring showed significantly increased hippocampal GR mRNA expression, enhanced glucocorticoid negative-feedback sensitivity, and decreased hypothalamic CRF mRNA levels.³⁹ Moreover, the magnitude of the corticosterone response to acute stress was significantly correlated with the frequency of both maternal LG (r=-0.61) during the first week of life, as was the level of hippocampal GR mRNA and hypothalamic CRF mRNA expression (all r values >0.70).³⁹

The offspring of the high- and low-LG mothers also differ in behavioral responses to stress.^40,41,68 As adults, the off spring of the high-LG mothers show decreased startle responses, increased exploration in novel, uncertain environments, and shorter latencies to eat food provided in a novel environment. The offspring of low-LG mothers also show greater burying of an electrified probe in the defensive burying paradigm,⁶⁸ which involves an active response to a clearly defined threat. The offspring of the high-LG mothers exhibit decreased CRF receptor levels in the locus ceruleus and increased γ-aminobutyric acid (GABA_A)/benzodiazepine (BZ) receptor levels in the basolateral and central nucleus of the amygdala, as well as in the locus ceruleus,^41,69 and decreased CRF mRNA expression in the central nucleus of the amygdala (Francis, Diorio, and Meaney, unpublished data). BZ agonists suppress CRF expression in the amygdala.⁷⁰ Predictably, stress-induced increases in PVNh levels of noradrenaline, which are normally stimulated by CRF, are significantly higher in the offspring of the low-LG offspring.⁷¹

Maternal care during the first week of life is associated with stable individual differences in GABA_A receptor subunit expression in brain regions that regulate stress reactivity. The adult offspring of high-LG mothers show significantly higher levels of GABA_A/BZ receptor binding in the basolateral and central nuclei of the amygdala, as well as the locus ceruleus. These findings provide a mechanism for increased GABAergic inhibition of amygdala-locus ceruleus activity. Importantly, maternal care also affects the behavioral sensitivity to acute BZ administration. The offspring of high-LG mothers show an increased anxiolytic response to acute BZ administration.

Recent studies⁴¹ suggest that variations in maternal care might actually permanently alter the composition of the GABA_A receptor complex in the offspring. The G ABA_A receptor is comprised of five individual protein subunits that collectively form a functional CI- channel mediating GABA-induced neuronal inhibition in the adult brain. There are over 20 individual subunits and variation in the function of the GABA_A receptor is associated with differences in the nature of the subunits comprising the receptor. Of particular interest are the a and y subunits, the presence of which defines a BZ binding site. The offspring of the high-LG mothers show increased levels of the mRNAs for the γ₁ and γ₂ subunits, both of which contribute to the formation of a functional BZ binding site. Such differences are not unique to the γ subunits. Levels of mRNA for the α subunit of the GABA_AA/BZ receptor complex are significantly higher in the amygdala and locus ceruleus of high-LG compared with low-LG offspring. The α₁ subunit appears to confer higher affinity for GABA, providing the most efficient form of the GABA_A receptor complex, through increased receptor affinity for GABA. The adult offspring of the low-LG mothers actually show increased expression of the mRNAs for the α₃ and α₄ subunits in the amygdala and the locus ceruleus. Interestingly, the GABA_A/BZ receptor composed of the α₃ and α₄ subunits show reduced affinity for GABA, by comparison with those containing an α subunit. Moreover, the α₄ subunit does not contribute to the formation of a BZ receptor site. These differences in subunit expression are highly specific to the amygdala and the locus ceruleus; no such differences are apparent in the hippocampus, hypothalamus, or cortex. Thus, differences in GABA_A/BZ receptor binding are not simply due to a deficit in subunit expression in the offspring of the low-LG mothers, but of an apparently “active” attempt to maintain a specific GABA_A/BZ receptor profile in selected brain regions.

Both postnatal handling, which increases maternal LG (see above), and rearing by a high-LG mothers enhance serotonin (5-hydroxytryptamine [5-HT]) turnover in the hippocampus in day-6 rat pups.^73,74 Interestingly, postnatal handling results in specific increases in 5-HT in the hippocampus and prefrontal cortex, where GR expression is increased.⁷⁴ 5-HT levels in the hypothalamus, septum, and amygdala are unaffected; GR levels in these regions are not altered by handling. Thus, the sensory input associated with maternal LG selectively alters 5-HT activity in specific brain regions.

The obvious question is whether the increase in 5-HT might directly influence GR gene expression. This issue is remarkably difficult to address with in vivo studies, in which pharmacological manipulations targeting a specific neurotransmitter system inevitably alter other systems, as well as systems in other brain regions. This issue begs an in vitro approach in which the relevant system, the hippocampal neurons, can be examined in a cell culture system. In vitro, the treatment of primary hippocampal cell cultures with 5-HT increases GR expression and this effect is mediated by 5-HT₇ receptor activation.^75-77 The 5-HT₇ receptor is positively coupled to cyclic adenosine monophosphate (cAMP) and GR expression in cultured hippocampal neurons is also significantly increased after treatment with 8-bromo-cAMP (a stable cAMP analog) or with various doses of the specific 5-HT₇ receptor agonists, such as 5-carboxamidotryptamine (5-CT). For all conditions, the effect on GR expression is apparent only after 4 days of treatment, a seemingly obscure fact whose importance will later become evidence. The effect of 5-CT on GR expression is blocked by methiothepin. Likewise, 5-CT produces a significant increase in cAMP levels and the effect is blocked by methiothepin. Pindolol, which binds to the 5-HT_1A, but not the 5-HT₇ receptor, has little effect (see also reference 76). These results further implicate the 5-HT₇ receptor. The intracellular effects of cAMP are commonly mediated by cyclic nucleotide-dependent protein kinases (PKA) and, predictably, a PKA inhibitor (H8) blocks the effects of 5HT or cAMP on hippocampal GR expression. Over the course of these studies, we found that other serotonergic agonists (quipazine, TFMPP [1-(trifluoromethylphenyl) piperazine], and DOI [(+/-)-2,5-demethoxy-4-iodoamphetamine]) could partially mimic the 5-HT effect on GR levels and, in all studies, the magnitude of the serotonergic effect on cAMP concentrations is highly correlated (r=0.97) with that on GR expression.⁷⁸ This observation is consistent with the idea that the effect of 5-HT on GR expression in hippocampal neurons is mediated by a 5-HT₇ receptor via activation of cAMP Importantly, both postnatal handling and increased maternal LG increase hippocampal concentrations of both cAMP and PKA in the rat pup. The conclusion of these studies provides the identification of an extracellular signal, 5-HT, and an intracellular, secondary messenger system, cAMP-PKA. Importantly, the in vivo effects of postnatal handling are blocked with compounds that serve as 5-HT₇ receptor antagonists.

The in vitro hippocampal cell culture system mimics the in vivo world with surprising authenticity. The increase in GR levels in cultured hippocampal neurons following 5-HT treatment persists following 5-HT removal from the medium; for as long as the cultures can be maintained, there is a sustained increase in GR levels as long as 50 days beyond the removal of 5-HT from the medium. Thus, 5-HT can act directly on hippocampal neurons to increase GR expression, and the effect of 5-HT on GR expression is observed in hippocampal culture cells mimics the long-term effects of early environmental events. These findings provide an in vitro “programming” model. Activation of cAMP pathways can regulate gene transcription through effects on a number of transcription factors, including, of course, the cAMP-response element binding protein (CREB) via an enhanced phosphorylation of CREB. In this instance, the second-messenger system alters the activity of the transcription factor, through enzymatic modification and phosphorylation, rather than production. CREB regulates gene transcription through pathways that involve the transcriptional cofactor, CREB -binding protein (CBP). Primary hippocampal cell cultures treated with 8-bromo-cAMP, 5-CT, or 5-HT show a significant increase in CBP expression.

The 5-HT₇ receptor is positively coupled to adenylyl cyclase, and thus the activation of cAMP In vivo, both handling and increased maternal LG result in an increased level of hippocampal cAMP concentrations and the activation of PKA over the first week of postnatal life.⁷⁶ Activation of PKA results in the tissue-specific induction of a number of transcription factors. The day6 offspring of high-LG mothers or pups of the same age exposed to handling show increased hippocampal expression of NGFIA (nerve growth factor-induced clone A, also known as zif-268, krox-24, egr-1, and zenk) (Weaver IGC et al, unpublished results).⁷⁹ In vitro, 5-HT increases NGFIA expression in cultured hippocampal neurons and the effect of 5-HT on GR expression in hippocampal cultures is completely blocked by concurrent treatment with an oligonucleotide antisense directed at the NGFIA mRNA.⁸⁰ The antisense is a synthetic strand of nucleotides that hybridizes with the native mRNA and prevents transcription of the NGFIA protein. These studies serve to identify a relevant transcription factor and to link the activation of the transcription factor NGFIA to the activation of GR expression in response to 5-HT.

Maternal LG results in an increased expression of NGFIA, which in turn might then regulate GR expression. Other rodent models examining environmental regulation of hippocampal GR expression also suggest a correspondence between NGFIA levels and GR expression.^81,82 In each case, increased levels of NGFIA are associated with enhanced GR expression. However, the critical site for GR regulation remains to be defined.

These findings provide a platform for the study of direct gene-environment interactions. However, the important missing piece is the identification of the relevant DNA target. We assumed that a potential target for regulation is the regulatory region of the GR gene. Regulatory regions contains sequences that alter the activity of the gene, such as promoters or suppressors, and are commonly found in front (or upstream) of the coding region of the gene that actually produces the protein. We identified and characterized several new GR mRNAs cloned from rat hippocampus (Figure 2).⁸³ All mRNAs encode a common protein, but differ in their 5 '-leader sequences presumably as a consequence of alternative splicing of, potentially, several different sequences from the 5' noncoding exon 1 region of the GR gene. In this case, the variation in the mRNAs reflects the different promoters that are spliced onto the coding region during transcription to create diverse GR mRNAs. The promoter, while spliced onto the mRNA, does not alter the translational phase by which mRNA is “translated” into the amino acid sequence that defines the protein product. The alternate exon 1 sequences are unlikely to alter the amino acid sequence of the GR protein; there is an “stop” codon present immediately 5' to the translation initiation site in exon 2, common to all the mRNA variants. Hence, only the coding region is actually translated into protein. Of the alternate exon 1 sequences identified, four correspond to exon 1 sequences previously identified in mouse, exons 1₁,1₅, 1₉, and 1₁₀ ^84,85 Most alternative exons are located in a 3-kb CpG island upstream of exon 2 that exhibits substantial promoter activity in transfected cells (Figure 2). Ribonuclease protection assays demonstrate significant levels of six alternative exon 1 sequences in vivo in the rat, with differential expression in the liver, hippocampus, and thymus presumably reflecting tissue-specific differences in promoter activity. The different promoters respond to different signals, which forms the basis for tissue-specific laterations in gene expression. Simply put, it is the process by which environmental or hormonal signals can alter GR expression in one region of the body, without affecting expression in another. Hippocampal RNA contains significant levels of the exon 1₇-containing GR mRNA variants expressed at undetectable levels in liver and thymus. These studies thus identify a brain-specific GR promoter, the exon 1₇ sequence.

In transient transfection experiments, a construct encoding the entire regulatory region of the GR gene, including eight of the alternate exon 1 sequences and the splice acceptor site within the intron 5' of exon 2, was fused to a lucif erase reporter gene. The lucif erase gene is activated by the coupled promoters and its activity thus reflects the ability of the regulatory sites to activate gene transcription - hence the term reporter gene. Fusion to the socalled reporter gene permits a measure of the degree to which individual sequences can potentially influence gene expression. This alteration in activity results from various sequences originating at any point within the regulatory region and, we presume, represents the sum of the activity of individual promoters on the genomic DNA fragment. In subsequent studies examining the potency of the individual promoters, we found that the relative activity of the individual exon 1 sequences is similar, with one notable exception, the exon 1₇ promoter sequence. The fused exon 1₇ has the highest transcriptional activity of any single promoter construct. More recent studies confirm the transactivational effect of NGFIA at the exon 1₇sequence. We used a cotransfection model with human embryonic kidney (HEK) cells (intentionally aiming as far from the neural target as possible) with an NGFIA expression vector and an exon 1₇-luciferase construct. Cotransfection of the NGFIA vector and the exon 1₇-luciferase construct resulted in a robust increase in luciferase activity, reflecting NGFIA-induced activation of transcription through the exon 1₇ promoter. These later studies reveal not only the ability of the exon 17 promoter to drive gene expressions, but that it does so in response to an increased NGFIA signal. Recall that an NGFIA antisense completely blocks the effects of 5-HT on GR expression in hippocampal cell cultures.⁸⁰

Interestingly, the activity of the exon 1₇ promoter is altered by postnatal handling, which increases GR expression in the hippocampus. Handling selectively elevated GR mRNA containing exon 1₇; there is, for example, no effect on exon 1₁₀.⁸⁵ Predictably, maternal care also affected the expression of GR splice variants: variants containing the exon 1₇ sequence were also significantly increased in the adult offspring of high-LG mothers (Weaver IGC et al, unpublished results). Thus, transcriptional activation of the GR gene in the hippocampus during adulthood is altered by maternal care over the first week of life.

The exon 1₇ promoter sequence of the GR gene contains guanine-cytosine nucleotides, so-called GC boxes (GCGGGGGCG), which form the core consensus site (ie, a DNA binding site) for NGFIA (Figure 2).⁸⁶ Thus, increases in NGFIA induced by maternal LG could increase transcription from the exon 1₇ promoter leading to increased GR mRNA. We previously found that handling increased the binding of NGFIA to a promoter sequence for the human GR promoter containing an NGFIA consensus sequence. Since neonatal handling increases maternal LG, these finding suggest that naturally occurring variations in maternal behavior might regulate GR expression in neonatal offspring through a 5HT-induced increase in NGFIA expression, and the subsequent binding of NGFIA to the exon 1₇ promoter. Recent findings support this idea, including studies using chromatin immunoprecipitation (ChIP) assay in which the in vivo formation of protein-DNA complexes are examined using cross-linking with paraformaldehyde perfusion and subsequent precipitation from soluble hippocampal samples using specific antibodies. Protein binding, defined by the specificity of the antibody, to specific DNA sequences is then quantified following polymerase chain reaction (PCR) amplification with targeted primers and Southern blotting. PCR allows for identification of precise DNA sequences and Southern blotting permits quantification of those same sequences. The experiment provides information on the amount of a specific DNA sequence bound to a specific protein. The charm of this approach is the ability to directly examine the interaction of specific proteins with specific DNA sequences at the time the biological sample is obtained. ChIP analysis of hippocampal samples from postnatal day-6 pups reveals dramatically increased NGFIA binding to the exon 1₇ promoter in the offspring of high-LG compared with low-LG mothers.⁶⁷ These findings confirm that maternal care regulates the binding of NGFIA to the exon 1₇ promoter sequence in pups.

The relation between DNA and histone is maintained, in part, by electrostatic bonds between positively charged histones and the negatively charged DNA. This chromatin structure commonly precludes transcription factor binding to DNA and underscores the importance of enzymes that modify histone-DNA interactions. Most modifications of the nucleosome occur on amino acid residues along the histone tail that protrudes through the DNA, and is thus vulnerable to enzymatic modification (Figure 3). The relevant histone modifications include acetylation, phosphorylation, ribosylation, and methylation. Each of these modifications can alter the interaction between the histones and the DNA, and thus alter gene expression. Our focus is on histone acetylation, which is closely associated with gene expression.

What distinguishes DNA methylation in vertebrate genomes is the fact that not all CGs within a common sequence are methylated in any given cell type.⁹⁵ Different CGs are methylated in different cell types, generating cell type-specific patterns of methylation. Thus, the DNA methylation pattern confers a cell-specific identity upon the genome. Such variation is presumably related to cell-specific patterns of gene expression. Since DNA methylation is part of the chemical structure of the DNA itself, it remains long after all other proteins and epigenomic markers are degraded and thus it has extremely important diagnostic potential.^96,97 It was originally believed that the DNA methylation pattern is established during development and is then maintained faithfully through life by the maintenance DNA methyltransf erase.^95,98 The DNA methylation reaction was believed to be irreversible and that the only way methyl residues were lost was through replication in the absence of DNA methyltransferase, resulting in the loss of the cytosine methylation in the daughter cell,^93,94 a mechanism that is not applicable to postmitotic cells such as neurons. However, recent findings together with the data reviewed here support an alternative model: one that suggests that the DNA methylation pattern is dynamic and is an equilibrium of methylation and demethylation reaction.^99,100We propose that DNA methylation is a reversible, like any other biological signal, and could potentially change in response to environmental and physiological signals.^99-101 The notion that DNA methylation is reversible in postmitotic cells has immense implications on our understanding the potential role of DNA methylation in marking gene expression in the brain.

The hallmark of DNA methylation patterns is the correlation between chromatin and the DNA methylation pattern: its importance for gene expression. Active chromatin is usually associated with unmethylated DNA, while inactive chromatin is associated with methylated DNA.^91,102,103 The reiation between DNA methylation, and chromatin structure (referring primarily to the relation between histone proteins and the DNA) has important implications for our understanding of the function of DNA methylation, as well as the processes responsible for generating, maintaining, and altering DNA methylation patterns under physiological and pathological conditions. It was originally believed that DNA methylation precedes and is dominant over chromatin structure.¹⁰⁴ Methylation was thought to be generated independently of chromatin structure. Over the course of development, methylation patterns were believed to be laid down shortly after cell replication and to then determine chromatin structure and gene expression. The DNA methylation pattern is proposed to guard the genome from random noise and drift.

Methylated DNA attracts methylated DNA binding proteins, which recruit a cluster of proteins referred to as repressor complexes, which include histone deacetylases that result in inactive chromatin and the silencing of gene expression.^105,106 The model positioning DNA methylation as driving chromatin inactivation is pervasive. Nevertheless, new data suggest that the state of chromatin structure can also determine DNA methylation and that chromatin can affect DNA methylation in both directions triggering either de novo DNA methylation or demethylation.^107-109 These data revise the classic model of a DNA methylation pattern that is determined during development and maintained through life, and adopt a more dynamic view of the DNA methylation pattern as an interface between the dynamic environment and the static genome. Thus, although DNA methylation is an extremely stable signal, it can be altered later in life when there is a sufficiently stable and consistent signal to activate the chromatin. Transient changes in cellular function and chromatin structure are not accompanied by changes in DNA methylation. The relation between chromatin state and DNA methylation forms a molecular link through which environmental signals might alter DNA methylation in specific genes in postmitotic neurons. Environmental signals trigger cellular signaling pathways, the downstream consequence of which is activation of trans-acting factors, such as transcription factors. These trans-acting factors recruit HATs to the target gene resulting in increased histone acetylation, chromatin opening, and increased accessibility of the DNA to demethylases. Since methylation of cytosine is an extremely stable chemical bond on DNA, this modification will remain stable for years. For methylation signals to serve as stable markers, they should not be responsive to transient chromatin noise or short-term signals. The mechanism proposed here also allows for a reversal of the methylation marker by a similar intense change in chromatin structure later in life.^99,110 This model has important implications on our understanding of how environmental signals, such as variations in maternal care, might stably alter glucocorticoid gene expression. DNA methylation marks genes for silencing by a number of mechanisms. The first mechanism is indirect and links DNA methylation to inactive chromatin structure. A region of methylated DNA juxtaposed to regulatory regions of genes attracts different members of a family of methylated DNA binding proteins, such as methylCpG-binding protein, MeCP2, which recruits HDACs^105,106 and histone methyltransferases¹¹¹ to methylated genes.^91,112 This results in a modification of chromatin around the gene precipitating an inactive chromatin structure. A different mechanism, which is relevant to our discussion here, involves direct interference of a specific methylated CpG residing within a response element for a transcription factor with the interaction of a transcription factor, such as the inhibition of binding of cMyc to its response element when it is methylated.¹¹³ Essentially, the methylated cytosine serves as a mutation of the recognition element, functionally reducing the binding affinity of the response element for its transcription factor. A third mechanism involves a combination of binding of a methylated DNA binding protein and inhibition of activity of a transcription factor.¹¹⁴ While the first mechanism is dependent on the general density of methyl cytosines within the region associated with a gene rather than methylation of a specific CpG, the second mechanism requires a discrete methylation event and is relevant to the mechanism proposed here.

The important consideration is the stability of cytosine methylation, which is preserved by covalent carbon-carbon bonds and could therefore serve as a long-term genomic “memory” of early experience influencing chromatin structure and GR expression in offspring of highand low-LG mothers. GR gene expression is increased throughout the hippocampus in the adult offspring of high-LG compared with low-LG mothers.³⁹ The exon 1₇ GR promoter sequence appears to be significantly more active in the adult offspring of high-LG compared with low-LG mothers and was therefore the focus of initial studies of possible maternal effects on DNA methylation. To test the hypothesis that maternal care alters the DNA methylation mark of the GR promoter, we⁶⁷ examined the level of methylation across the entire exon 1₇ GR promoter sequence in the hippocampus using the sodium bisulfite (NaBis) mapping technique in the adult offspring of high- and low-LG mothers. NaBis treatment of DNA samples converts nonmethylated cytosines to uracils, which are then detected as thymidine on subsequent sequencing gels.¹¹⁵ Methylated cytosines are unaffected by NaBis and the differences in methylation status are thus apparent and easily quantifiable on sequencing gels. We found significantly greater methylation of the exon 1₇ GR promoter sequence in the offspring of the low-LG mothers. These findings are consistent with the hypothesis that maternal effects alter DNA methylation patterns in the offspring.

To determine whether DNA methylation of specific target sites on the GR promoter change in response to maternal care, we mapped the differences in methylation of individual cytosines, focusing on a region around the NGFIA consensus sequence within the exon 1₇ promoter. The results reveal significant differences in the methylation of specific regions of the exon 1₇ GR promoter sequence. Notably, the cytosine within the 5' CpG dinucleotide of the NGFIA consensus sequence (Figure 2) is always methylated in the offspring low-LG mothers, and rarely methylated in the offspring of high-LG dams. This is consistent with site-specific DNA methylation silencing of the GR promoter.

This is an important finding for our understanding of the processes by which maternal care programs hippocampal GR expression and thus HPA responses to stress. While there are substantial differences in differences in NGFIA expression between the offspring of high- and low-LG mothers in early postnatal life, no such differences are apparent in adulthood. Our hypothesis is that the cytosine methylation in the response element for NGFIA interferes with NGFIA binding to the GR exon 1₇ promoter. We therefore predicted that the reduced cytosine methylation in the adult offspring of high-LG compared with low-LG mothers would result in greater NGFIA binding to the exon 1₇ promoter. This prediction was confirmed using a ChIP assay (described above) examining in vivo formation of protein-DNA complexes in hippocampal tissue from adult animals.⁶⁷ The results indicated a threefold greater binding of NGFIA protein to the hippocampal exon 1₇ GR promoter in the adult offspring of high-LG compared with low-LG mothers. Using the same tissue samples and an antibody against the acetylated form of H3, we⁶⁷ found dramatically increased acetylated H3 association with the exon 1₇ GR promoter in the offspring of the high-LG mothers. As described above, histone acetylation is associated with active states of gene expression. These findings are therefore consistent with the idea of increased NGFIA binding to the exon 1₇ promoter, enhanced histone acetylation, and increased GR transcriptional activation.

The differences in the status of methylation of the exon 1₇ GR develop between the two groups emerges between postnatal day 1 and 6, which is precisely the period when differences in the maternal behavior of high- and low-LG dams are apparent. There are no differences in maternal LG between high- and low-LG mothers beyond day 8.^65,69 By postnatal day 6, the 5' CpG dinucleotide of the NGFIA response element is demethylated in the highLG, but not in the low-LG group. These findings are consistent with data from the cross-fostering experiment, which illustrates that the differences between the two groups developed following birth in response to maternal behavior. The group difference in CpG dinucleotide methylation then remains consistent through to adulthood. Our findings suggest that the group difference in DNA methylation occurs as a function of a maternal behavior over the first week of life. The results of earlier studies indicated that the first week of postnatal life is indeed a critical period for the effects of early experience on hippocampal GR expression.¹²¹

The striking finding from this rather simple study was evidence of a demethylation, as opposed to the prevention of methylation. A recent paper on altered expression of interleukin-2 (IL-2) expression T lymphocytes following activation also clearly implicates an active process of demethylation in a normal differentiated somatic cell. Bruniquel and Schwartz¹²² found that a region in a promoter of the IL-2 gene demethylates following activation in the absence of DNA replication and results in a profound increase in the production of IL-2. These two papers provide the initial evidence for an active, environmentally driven alteration in DNA methylation in postmitotic cells. Szyf and colleagues^101,123 first proposed that DNA methylation is enzymatically reversible and that DNA methylation is dynamic in fully differentiated cells. This idea remains controversial. Active demethylation was nevertheless clearly demonstrated early in embryogenesis and the parental genome undergoes replication independent, active demethylation hours after fertilization, well before the initiation of replication. Demethylation at very early stages in development has been relatively accepted, but the possibility of postnatal demethylation, especially in fully differentiated somatic cells, has been hotly disputed. However, active replication demethylation was demonstrated in Epstein-Barr virus (EB V)-infected B cells and in HEK293 cells. The HEK293 studies suggest that active replication-independent demethylation takes place in differentiated somatic cells and that it is dependent on alterations in chromatin structure.

Earlier studies from Szyf's¹²² laboratory extracted active DNA demethylase activity from a human lung cancer cell line and identified a protein with demethylase activity, which was cloned concurrently by Bird's group and named MBD2.¹²³ Interestingly, the protein, MBD2, was found by Bird's group and others to also associate with a chromatin remodeling complex containing HDAC, which is involved in silencing of gene expression through the recruitment of a repressor complex. The assignment of a demethylase function to a protein that was independently discovered as a recruiter of repressor complexes triggered the expected controversy in the field and reports that MBD2 failed to produce demethylase activity. However, the observation that MBD2/demethylase expression produces the demethylation of some, but not all, promoters in a dose- and time-dependent manner has been confirmed.^108,124 Clearly, the contextual factors that determine MBD2 demethylase activity remain to be fully explained. Interestingly, MBD2 increased gene expression in those instances where promoter demethylation occurred, suggesting that not all promoters respond in the same orderly manner. Indeed, the same is true for DNA methylation, which impedes the DNA binding of most, but not all transcription factors; SP1 binds to methylated DNA. Antisense knock down of MBD2 resulted in inhibition of active demethylation induced by valproate and caused hypermethylation and silencing of the prometastatic gene uPA in metastatic breast cancer cells. Another group reported that ectopic expression of MBD2/demethylase in hepatocyte cell line caused demethylation and activation of the hexokinase type 2 gene.¹²⁵ Additional support for the demethylase activity of MBD2/demethylase emerges from the finding that expression of MBD2/demethylase is correlated with demethylation within the promoters of C-ERBB-2 and SURVIVIN genes in ovarian cancers^126,127 and hypomethylated CMYC in gastric cancer.¹²⁸ In addition, the Drosophila homolog of MBD2, dMBD2/3, formed foci that associated with DNA at the cellular blastoderm stage, concurrent with the activation of the embryonic genome, and also associated with the active Y chromosome.¹²⁹

These findings beg the question of how maternal high LG might activate a demethylation of the GR exon 1₇ promoter. A testable working hypothesis is that high LG leads to activation of NGFIA as a downstream effector of activation of a 5-HT signaling through increase cAMP and PKA. Increased NGFIA increases NGFIA binding to the GR exon 1₇ promoter. The interaction of NGFIA with the GR exon 1₇ promoter leads to increased histone acetylation and increased accessibility of the GR exon 1₇ promoter to demethylase resulting in DNA demethylation. In contrast, in the absence of increased NGFIA during early postnatal life, the 5' CpG site of the NGFIA response element remains methylated and significantly less sensitive to NGFIA over the life span. The methylation of the 5' CpG site is thought to preclude NGFIA binding through the participation of a repressor complex that includes methylated DNA binding proteins and HDACs. This hypothesis predicts that pharmacological activation of chromatin using HDAC inhibitors should result in activation of NGFIA binding and GR exon 1₇ promoter demethylation. However, the question is whether reversibility reflected in demethylation is limited to early life exclusively or whether it is possible to reverse these marks later in life as well if the appropriate signals to activate the chromatin structure are applied or by a pharmacological activation of chromatin structure. Our hypothesis is that the DNA methylation is a steady state of DNA methylation and demethylation whose direction is determined by the state of chromatin structure.^{99 ,110} This hypothesis predicts that both DNA methyltransferases and demethylases are present in adult neurons and that if the chromatin state is altered by either persistent physiological or pharmacological signals one should be able to change the state of methylation of a gene in postmitotic tissue, such as adult hippocampal neurons. We previously established that pharmacological activation of chromatin structure by HDAC inhibitors can trigger replication-independent active demethylation of DNA.^108,130,131 We tested our hypothesis that the demethylation of the GR exon 1₇ promoter is driven by histone acetylation and could be activated in adult neurons as well; HDAC inhibition should reverse the effects of cytosine methylation on NGFIA binding to the exon 1₇ promoter, GR expression, and HPA responses to stress. We used a central infusion of adult offspring of high- or low-LG mothers with the HDAC inhibitor, trichostatin A (TSA), for 4 consecutive days. As expected, ChIP assays revealed that HDAC inhibition through TSA infusion significantly increased the level of acetylated H3 at the exon 1₇ site (ie, HDAC inhibition resulted in increased histone acetylation) in the offspring of lowLG mothers to levels comparable to those observed in the offspring of high-LG mothers. The increased histone acetylation is associated with enhanced NGFIA binding to the exon 1₇ promoter sequence and completely eliminates the effect of maternal care. As expected, enhanced NGFIA binding to the exon 1₇ promoter increased hippocampal GR expression. Hippocampal GR expression in the TSA-treated adult offspring of low-LG mothers was indistinguishable from that of the high-LG groups. Most important, TSA infusion eliminated the effect of maternal care on HPA responses to stress. During and following exposure to acute stress, plasma corticosterone levels in TSA-treated offspring of low-LG mothers are indistinguishable from those of TSA- or vehicle-treated high-LG mothers. There was no effect of TSA on any measure in the offspring of high-LG animals. This is understandable since under normal circumstances there is considerable H3 acetylation and NGFIA binding at the exon 1₇ sequence in these animals. Interestingly, TSA treatment also led to the demethylation of the 5' CpG of the NGFIA response element of the GR exon 1₇ promoter sequence.

These findings have important implications for our understanding of the mechanisms linking early maternal behavior and stable changes in behavior later in adulthood as well as on our understanding of the mechanisms responsible for maintaining the DNA methylation pattern in adult postmitotic tissues.