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Invest Ophthalmol Vis Sci. Aug 2011; 52(9): 6572–6579.
Published online Aug 22, 2011. doi:  10.1167/iovs.10-7082
PMCID: PMC3176028
The Role of Vldlr in Intraretinal Angiogenesis in Mice
Chun-hong Xia,corresponding author1 Eric Lu,1 Haiquan Liu,1 Xin Du,2 Bruce Beutler,2 and Xiaohua Gong1,3
From the 1School of Optometry and Vision Science Program, and
the 3Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, California; and
the 2Department of Genetics, The Scripps Research Institute, La Jolla, California.
corresponding authorCorresponding author.
Corresponding author: Chun-hong Xia, 695 Minor Hall, University of California, Berkeley, CA 94720-2020; chxia/at/berkeley.edu.
Received December 17, 2010; Revised May 23, 2011; Accepted June 16, 2011.
Purpose.
To identify and characterize the r26 mouse line, which displays depigmented patches in the retina, and to determine the causative gene mutation and study the underlying mechanism.
Methods.
Fundus examination, fluorescein angiography, histology, and immunostaining were used to determine the retinal phenotypes. Genome-wide linkage analysis, DNA sequencing, and an allelic test were used to identify the causative gene mutation. Wild-type and mutant gene products were examined by Western blot and transient transfection.
Results.
Homozygous r26/r26 mice displayed depigmented patches in the fundus that overlapped the hyperfluorescent spots in the angiogram. Histology showed overgrown retinal vessels in the subretinal space. Immunostaining verified the presence of endothelial cells in the photoreceptor layer. Chromosome mapping and DNA sequencing revealed a point mutation, c.2239C>T, in the very-low-density lipoprotein receptor (Vldlr) gene. An allelic test in Vldlr knockout (−/−) mice confirmed that r26/ mice display a phenotype similar to that of r26/r26 mice. The Vldlr protein was predominantly localized at the plasma membrane of transfected cells, whereas the truncated Vldlr was diffusely expressed in the cell cytosol. The r26 truncated Vldlr was undetectable in mutant retinas by Western blot.
Conclusions.
The r26 is a recessive mutant caused by a missense mutation in the Vldlr gene. This results in a truncated Vldlr protein that lacks the C-terminal 127 amino acid residues including the single transmembrane domain and fails to localize at the plasma membrane. Thus, the r26 is a loss-of-function Vldlr mutation. Vldlr on the cell surface probably mediates an antiangiogenic signal to prevent retinal endothelial cells from migrating into the photoreceptor cell layer.
Retinal vasculature is essential for normal visual function of the eye. Vascular abnormalities, including ischemia, neovascularization, and vessel leakage occur in common eye diseases such as diabetic retinopathy, age-related macular degeneration (AMD), and macular edema, which are some of the leading causes of blindness in adults.13 Retinal angiomatous proliferation (RAP), characterized by subretinal neovascularization originating from retinal vessels, is a subtype of AMD and accounts for 10% to 15% of neovascular AMD.48 Recent studies have reported that very-low-density lipoprotein receptor (Vldlr) knockout mice replicate some characteristic phenotypes of human RAP, suggesting that Vldlr mutant mice can be used as animal models for studying the underlying mechanism of the development of RAP.913
Vldlr knockout (−/−) mice develop retinal angiomatous proliferation with subretinal neovascularization arising from retinal vessels.1013 In Vldlr−/− mice, retinal vessels grow into the subretinal space, which results in retinal–choroidal anastomosis, subretinal fibrosis, retinal pigment epithelium (RPE) hyperplasia, and photoreceptor degeneration. The loss of Vldlr facilitates the proliferation, migration, and capillary-like formation of retinal vascular endothelial cells and enhances the angiogenic properties of endothelial cells in vitro and in vivo.14 Thus, Vldlr negatively regulates retinal angiogenesis during development. Moreover, recent findings have indicated Vldlr as one of the functional candidate genes in human AMD.15 However, the underlying mechanism for how the Vldlr mutation promotes retinal angiogenesis remains unclear; it is also unclear whether this retinal phenotype is associated with the function of Vldlr as a receptor for the uptake of very-low-density lipoprotein (VLDL) or as a mediator for other intracellular signaling.
We report the identification and characterization of a new recessive Vldlr mutation, r26, from N-ethyl-N-nitrosourea (ENU)-mutagenized mice. The r26 mutant mice contain a missense point mutation of the Vldlr gene, leading to the expression of a truncated Vldlr protein with a premature termination at amino acid residue 747. Similar to the Vldlr−/− mice, the r26 homozygous mice display uncontrolled intraretinal angiogenesis. This work further demonstrates the essential role of the C terminus of Vldlr in the regulation of intraretinal angiogenesis. This truncated Vldlr mouse line is a useful model for the study of the underlying mechanism for Vldlr signaling and human RAP.
Animals, Fundus Photography, and Fluorescence Angiography
Animals were cared for in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research, and all studies were conducted in accordance with a protocol approved by the Animal Care and Use Committee (ACUC) at the University of California, Berkeley. ENU-mutagenesis and mouse breeding were performed as previously described.16,17 Allelic testing was conducted by crossing the homozygous r26 mutant mice with the homozygous Vldlr−/− mice.
Fundus photography and fluorescence angiography were performed as described previously.17
Immunofluorescent Staining, Vessel Painting, and Retinal Histology Analysis
Immunofluorescent staining and vessel-painting experiments were performed as described previously.17 For immunostaining, a rat anti-CD31 antibody (BD Pharmingen, San Diego, CA) was used to stain retinal vessels in frozen sections, and a goat anti-Vldlr antibody (R&D Systems, Minneapolis) was used to stain transfected cells. For vessel-painting experiments, a solution of 0.1 mg/mL of lipophilic dye DiI (Sigma-Aldrich, St. Louis, MO) was perfused transcardially into the animals, and flat-mounted retinas were imaged using a fluorescent microscope (Zeiss Axiovert 200 equipped with Apotome; Carl Zeiss, Jena, Germany).
Retinal histology analysis was performed as described previously.17
Chromosome Mapping and Sequencing Analysis
For genome-wide linkage analysis, homozygous r26 mutant mice in C57BL/6J strain background were mated to wild-type C3H/HeN mice to generate heterozygous hybrid mice. The backcrossed mice were generated by crossing the heterozygous hybrid mice with homozygous r26 mutant mice. Backcrossed mice were phenotyped, and tail DNAs were genotyped. A total of 59 microsatellite markers were used.
For sequencing analysis, total RNA was isolated from the retinas of homozygous r26 mutant mice (TRIzol reagent; Invitrogen Life Technologies, Carlsbad, CA), and cDNAs were synthesized as described previously.17 The Vldlr coding region was then amplified (Platinum pfx DNA polymerase; Invitrogen Life Technologies). PCR fragments with overlapping regions were sequenced with various primers spanning the whole coding sequence of Vldlr.
Western Blot Analysis
Four retinas were pooled from two mice with the same genotype and were homogenized in the lysis buffer (20 mM Tris [pH 7.5], 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100) containing proteinase inhibitors (Roche Diagnostic Corporation, Indianapolis, IN). The protein lysates were sonicated, and protein concentration was determined (Coomassie Assay Reagent kit; Pierce, Rockford, IL). The samples were then mixed with an equal volume of 2× loading buffer (120 mM Tris-PO4 [pH 6.8], 4% SDS, 20% glycerol, 10% β-mercaptoethanol, and 0.002% bromophenol blue). Equal amounts (150 μg protein) of the samples were loaded onto 4% to 12% Bis-Tris gel (NuPAGE; Invitrogen, Carlsbad, CA) for separation, and the gel was transferred to PVDF membrane (Bio-Rad Laboratories, Hercules, CA). A goat anti-Vldlr antibody (R&D Systems) and a mouse anti-β-actin antibody (Sigma-Aldrich) were used to detect the specific proteins, and chemiluminescence (SuperSignal West Pico Chemiluminescent substrate kit; Thermo Scientific, Rockford, IL) was used to develop the blots.
Construction of Vldlr Expression Plasmids and Transient Transfection In Vitro
Two plasmids expressing full-length Vldlr or truncated r26 mutant Vldlr were constructed. RNA isolated from the retinas of C57BL/6J wild-type mice was used to synthesize cDNA, and the cDNA was used as the template for PCR amplification of the full-length Vldlr and truncated r26 Vldlr fragments. The primer pair of 5′-EcoRI-GCATGGGCACGTCCGCGC-3′ and 5′-SacII-AGCCAGATCATCATCTGTGC-3′ generated a DNA fragment that spans the ATG and the last codon (before the stop codon) of the Vldlr, and this fragment was then subcloned into the EcoRI and SacII sites of the mEos2-N1 vector; the primer set of 5′-EcoRI-GCATGGGCACGTCCGCGC-3′ and 5′-SacII-TCCATTTTCTTCGAGATTG-3′ amplified a truncated DNA fragment including the ATG and the codon before the stop codon caused by the r26 mutation, and the fragment was also subcloned into the EcoRI and SacII sites of the mEos2-N1 vector. In both constructs, the mEos2 protein is in frame with the N-terminal Vldlr protein.
Plasmid DNAs of Vldlr/mEos2-N1 and r26-Vldlr/mEos2-N1 were transiently transfected into HEK293 cells in vitro (Superfect transfection reagent; Qiagen, Valencia, CA). Briefly, HEK293 cells (1.4 × 105) were seeded in 35-mm glass dishes in MEM complete medium, and transfection was performed 24 hours after cell plating. The expression of full-length or r26 mutant Vldlr was examined by confocal microscope (Carl Zeiss) 24 or 48 hours after transfection.
Depigmented Patches in the r26/r26 Mutant Fundus
Using an indirect ophthalmoscope, we observed that a male mouse from the G3 generation of ENU-mutagenized mice displayed depigmented patches in the retina. This G3 male founder was mated with wild-type C57BL/6J female mice to generate the r26 mutant mouse line. We confirmed by fundus examination that homozygous r26/r26 mutant mice developed depigmented patches in the retina (Figs. 1B, B,1C),1C), whereas heterozygous r26/+ littermates had normal retinas (Fig. 1A). Moreover, fluorescein angiography revealed that depigmented patches overlapped with hyperfluorescent spots in the r26/r26 mutant (Figs. 1C, C,11D).
Figure 1.
Figure 1.
Fundus photographs show depigmented patches in the r26 homozygous mutant mice. In comparison to the fundus image from a 3-week-old heterozygous r26/+ control mouse (A), the homozygous r26/r26 mutant mouse at the same age displayed depigmented patches (more ...)
Retinal Histology
Histology analysis was performed to examine the morphologic changes in r26 mutant retinas. At the age of 4 weeks, compared to the wild-type retina (Fig. 2A), the r26/r26 mutant displayed all retinal layers without any obvious loss of photoreceptor cells (Fig. 2B). However, some areas of the retinal outer nuclear layer (ONL), the subretinal space, and the retinal pigment epithelium (RPE) were distorted, with the presence of vascular-like cells (Figs. 2B, B,2C,2C, arrows). Changes and degeneration of photoreceptor cells became apparent as r26/r26 mutant mice grew older. At the age of 2 months, some photoreceptor cells completely lost their outer- and inner-segments, and the ONL was adjacent to the RPE in the r26/r26 retina (Fig. 2D). At the age of 14 months, severe photoreceptor cell degeneration was observed in r26/r26 mutant mice; moreover, vessellike cellular structures appeared in the disorganized subretinal region near the RPE layer (Figs. 2E, E,2F,2F, arrows).
Figure 2.
Figure 2.
Histology of r26 mutant retinas. Compared with the 4-week-old wild-type +/+ control (A), the homozygous r26/r26 mutant at the same age showed a relatively normal number of photoreceptor nuclear layers (B). However, some areas of the ONL were distorted, (more ...)
Abnormal Intraretinal Vascular Growth beyond the Outer Plexiform Layer
Immunostaining with the endothelial cell–specific CD31 antibody and vascular perfusion with a fluorescent dye (vessel painting) were used to examine the retinal vasculature in r26/r26 homozygous mutant mice by comparing it to that of the wild-type or r26/+ heterozygous control. In 3-week-old wild-type control mice, CD31 fluorescence signals showed that retinal vascular endothelial cells were restrictively present in the outer plexiform layer (OPL), inner plexiform layer (IPL), and ganglion nerve fiber layer (GL), but not in the photoreceptor ONL and beyond. However, in r26/r26 mutant retinas, CD31-positive vascular endothelial cells were detected, not only in the OPL, IPL, and GL, but also in the ONL (Fig. 3A, arrowheads) and the subretinal space (data not shown). Thus, immunostaining revealed uncontrolled growth of retinal vessels in the photoreceptor cell layer and subretinal space of r26/r26 mutant retinas.
Figure 3.
Figure 3.
Abnormal retinal vessel growth in the r26 mutant retina. (A) Immunostaining of 3-week-old wild-type and r26 mutant retinas. In the wild-type control retina, vascular endothelial cells stained by an anti-CD31 antibody were observed in the OPL, IPL, and (more ...)
Three-dimensional (3D) reconstructions of retinal vasculature were obtained from z-stack fluorescent images of whole-mounted retinas isolated from the control r26/+ and mutant r26/r26 mice, which had been transcardially perfused with a lipophilic fluorescent dye.17 The front views of 3D images of retinal vasculature showed no obvious difference between heterozygous r26/+ control and homozygous r26/r26 mutant mice (Fig. 3B, top). However, a cross-sectional 3D view (90° rotation of the front view) revealed that a normal three-layer retinal vasculature, located in the GL, IPL, and OPL, was present in the r26/+ control (Fig. 3B, bottom left), whereas the r26/r26 mutant displayed abnormal vessels that extended beyond the OPL toward the subretinal space (Fig. 3B, bottom right, arrow). Both CD31 immunostaining data of frozen retinal sections and the 3D images of fluorescent dye–perfused retinal vasculature clearly demonstrated that the overgrowth of retinal vasculature is the cause of abnormal vessels in the photoreceptor cell layer and the subretinal space of the r26/r26 mutant mice.
A Vldlr Missense Mutation in r26/r26 Mice
To identify the causative gene mutation in the r26 mutant mouse line, we performed genomewide linkage analysis. Fifty-nine microsatellite markers were tested among 30 meioses, and the r26 locus was confined to chromosome 19 with a peak LOD score of 4.8 (Fig. 4A). A previous study reported similar retinal phenotypes in the Vldlr−/− mice,10 and the Vldlr gene resides on chromosome 19.18 Thus, we performed sequence analysis of the Vldlr gene in r26 mutant mice. Total RNA was isolated from r26/r26 mutant retinas and subsequently used to synthesize cDNA, and the coding region of Vldlr was amplified and sequenced. A change of C to T at nucleotide 2239 of the Vldlr coding sequence was identified. This single nucleotide change resulted in a premature stop codon at amino acid residue 747 of the Vldlr protein. The missense mutation was predicted to express a truncated Vldlr protein with the first 746 amino acids but without the C-terminal 127 amino acid residues that include a single transmembrane domain.
Figure 4.
Figure 4.
(A) Genome-wide linkage analysis. The r26 locus is confined to chromosome 19 with a peak LOD score of 4.8 on 30 meioses. Fifty-nine microsatellite markers were used. (B) An allelic test between Vldlr−/− and r26 mutant mice. As shown by (more ...)
To verify the functional change of this truncated Vldlr mutant, we performed an allelic test by crossing homozygous Vldlr−/− and r26/r26 mice. Interestingly, the r26/ mice developed a retinal vascular phenotype similar to r26/r26 and Vldlr−/− mice, examined by indirect ophthalmoscope and fluorescein angiography (data not shown). Retinal histology of a 6-week-old r26/ mouse showed abnormal vascular growth from the OPL toward the subretinal space and RPE layer (Fig. 4B); this defect was identical with that in the r26/r26 mutant retina. Thus, the r26 mouse is a loss-of-function mutation of Vldlr.
Western blot analysis was performed to examine the expression of the wild-type Vldlr and the r26 truncated Vldlr proteins in retinal homogenates (Fig. 5A). The full-length Vldlr protein was detected in the retinas of the wild-type mice (w1 and w2 in Fig. 5A). As expected, Vldlr protein was not detected in the retinas from Vldlr−/− mice (k1 and k2). Moreover, the truncated Vldlr protein (r1 and r2) was undetectable in the r26/r26 retinal homogenates.
Figure 5.
Figure 5.
(A) Western blot to examine Vldlr expression in wild-type (w), r26/r26 (r), and Vldlr−/− (k) retinas. Retinas from two wild-type mice (w1 and w2) expressed the full-length Vldlr protein. The Vldlr−/− mice (k1 and k2) had (more ...)
To further characterize the r26 Vldlr mutation, we constructed two expression plasmids that express either the full-length Vldlr tagged with mEos2 fluorescent protein or the r26 truncated Vldlr tagged with mEos2 fluorescent protein. Both plasmids were transiently transfected into HEK293 cells in vitro. As shown in Figure 5B, 24 hours after transfection, the full-length Vldlr protein was predominantly localized in the plasma membrane (as visualized by mEos2 fluorescent protein in green), whereas the r26 truncated Vldlr protein was mainly localized in intracellular regions. Forty-eight hours after transfection, the plasma membrane enriched localization of the full-length Vldlr was revealed by both the tagged fluorescent protein and immunostaining signals using a Vldlr antibody (Fig. 5C, top), whereas a diffuse cytosolic distribution of the truncated r26-Vldlr was observed in transfected cells (Fig. 5C, bottom). Thus, unlike the full-length Vldlr protein, the r26 truncated Vldlr protein fails to localize at the plasma membrane.
We have identified an ENU-induced r26 mouse mutant, which is a missense mutation of the Vldlr gene, a c.2239C>T for a p.R747X substitution. This mutation results in a truncated Vldlr protein lacking the transmembrane domain. The r26 mutant mice develop subretinal neovascularization similar to that of Vldlr−/− mice. Therefore, the r26 mouse line is a loss-of-function mutation of Vldlr. However, unlike the Vldlr−/− mice that lack the expression of Vldlr protein in vivo, the r26 mutant mice probably express a soluble form of Vldlr. Thus, the r26 mutant line may be a useful and complementary model for investigating the role of Vldlr signaling in the regulation of retinal angiogenesis in vivo. A recent study has reported an increased proliferation and migration of Vldlr−/− retinal endothelial cells.14 However, the molecular and cellular mechanisms for how Vldlr controls intraretinal angiogenesis during development remain largely unknown.
Vldlr belongs to the evolutionarily conserved LDL receptor family, and it contains typical structural/functional domains like many other members of the LDL receptor family. Vldlr can be structurally divided into three regions: the extracellular region that includes ligand-binding repeats domain, the EGF-like repeat domain, the YWTD β-propeller domain, and the O-linked glycosylation domain; a single transmembrane domain region; and a short cytosolic tail region that contains the NPXY motif (Fig. 6).19,20 Vldlr was initially thought to be an endocytic receptor that mediates the binding and uptake of apoE-containing lipoproteins, such as VLDL and β-VLDL.21 Early studies reported that homozygous Vldlr−/− mice had normal lipoprotein profiles without any significant phenotypes, except that the animals were somewhat smaller and leaner at a young age.9 Interestingly, recent findings reveal that retinal vasculature defects are the main phenotype observed in the Vldlr−/− mice.1013 Vldlr may function in endothelial cells as an endocytic receptor to mediate the endocytosis of cell surface-bound ligands that may act as proangiogenic factors in the retina.
Figure 6.
Figure 6.
Structural domains of the Vldlr protein. In the wild-type protein, the ligand-binding repeats domain, the EGF-like repeats domain, the YWTD β-propeller domain, and the O-linked glycosylation domain are located in the extracellular space; a single (more ...)
However, Vldlr has been demonstrated to play a signaling function by its interaction with Reelin to modulate neuronal migration, neurodevelopment, and other physiological processes in the central nervous system.22 This has been well documented by the Reelin-Vldlr/ApoER2 signaling pathway. Reelin is a large secreted protein that is primarily expressed in nerve tissues. Vldlr and ApoER2 are functional Reelin receptors. Defective Reelin signaling leads to disordered laminated cortical structure.23 Indistinguishable phenotypes were uncovered among the ataxic reeler mutant strain,24 Disabled-1 (dab1) gene mutant mice,25,26 and mice lack both Vldlr and ApoER2,27 suggesting that Reelin, Dab1, Vldlr, and ApoER2 function in the same signaling pathway. Reelin signaling is initiated by the binding of Reelin to both Vldlr and ApoER2, and the ligand/receptor binding triggers the tyrosine phosphorylation of Dab1, thus regulating neuronal migration.28 Dab1 binds to the NPXY motif in the cytosolic domain of ApoER2 and Vldlr.29 The NPXY motif is essential for Reelin signaling, since it is the critical structural domain for Dab1 binding to both receptors.30 It has been reported recently that Vldlr-mediated Reelin signal prevents neurons from entering the marginal zone, suggesting that Vldlr provides a stop signal for migrating cortical neurons.31 However, Dab1 expression is limited to neurons, and it is unclear whether Dab1 or Dab2 is expressed in retinal endothelial cells.32 Therefore, the downstream targets of Vldlr that mediate endothelial cell migration in angiogenesis need to be explored. It is also possible that other cell types expressing Vldlr in the retina may be the key in the regulation of retinal angiogenesis.
The r26 mutation leads to a premature stop codon at amino acid residue 747 of the Vldlr protein. The truncated Vldlr-R747X protein contains only the first 746 amino acid residues and lacks the C-terminal 127 amino acids that include the single transmembrane domain. Unlike the plasma membrane localization of the full-length Vldlr protein, the r26 truncated Vldlr protein displays an intracellular distribution in transfected cells in vitro. As shown in Figure 6, the r26 truncated Vldlr-R747X protein lacks the transmembrane domain. It is also possible that the truncated proteins will be secreted into the extracellular space in vivo. Regardless of the intracellular or extracellular distribution of the r26 truncated Vldlr protein in vivo, the expression level of this truncated Vldlr must be extremely low in vivo, since its protein amount in the retinal homogenates is undetectable by Western blot. Thus, the r26 mutant line is a valuable model for a loss-of-function mutation of Vldlr.
Previous reports of Vldlr−/− mice and our present study have demonstrated that Vldlr loss-of-function mutations lead to an uncontrolled overgrowth of intraretinal vessels.1013 Vldlr provides an essential cue to prevent retinal vascular endothelial cells from migrating into the photoreceptor cell layer. It is unknown what triggers the migration of endothelial cells into photoreceptor/subretinal space in the Vldlr mutants and what the Vldlr ligand is in retinal endothelial cells.
We hypothesize that Vldlr binds an anti-angiogenic factor to prevent the endothelial cells from migrating into the photoreceptor layer. Further investigation is needed to understand whether Vldlr has a co-receptor like ApoER2 in a Reelin-like signaling pathway in the retina and which intracellular downstream molecules, such as Dab1-like molecules, are involved in this signaling pathway. It is also necessary to determine whether Vldlr is expressed in other retinal cells besides endothelial cells to inhibit the vessel growth in photoreceptor cells or subretinal space. A better understanding of the Vldlr signaling pathway will be very helpful for developing new therapeutic methods for treating certain types of AMD, such as RAP, with abnormal intraretinal angiogenesis.
Acknowledgments
The authors thank Bo Chang for helpful discussions and for sharing the Vldlr−/− mice (B6;129-Vldlrtm1Her(r)) and Jeffrey Tsao for assistance with the transfection experiments.
Footnotes
Supported in part by Grant EY013849 (XG) from the National Eye Institute and a grant from the East Bay Community Foundation.
Disclosure: C. Xia, None; E. Lu, None; H. Liu, None; X. Du, None; B. Beutler, None; X. Gong, None
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