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The cancer-testis antigen SSX-2 is a potentially attractive target for tumor immunotherapy based upon its tissue-restricted expression to germline cells and its frequent expression in malignancies. The goal of the current study was to evaluate a genetic vaccine encoding SSX-2 to prioritize HLA-A2-specific epitopes and determine if a DNA vaccine can elicit SSX-2-specific cytolytic T lymphocytes (CTL) capable of lysing prostate cancer cells. HLA-A2-restricted epitopes were identified based on their in vitro binding affinity for HLA-A2 and by the ability of a genetic vaccine to elicit peptide-specific CTL in A2/DR1 (HLA-A2.1+/HLA-DR1+/H-2 class I-/class II-knockout) transgenic mice. We found that SSX-2 peptides p41-49 (KASEKIFYV) and p103-111 (RLQGISPKI) had high affinity for HLA-A2 and were immunogenic in vivo, however peptide p103-111 was immunodominant with robust peptide-specific immune responses elicited in mice vaccinated with a plasmid DNA vaccine encoding SSX-2. Furthermore, p103-111-specific CTL were able to lyse an HLA-A2+ prostate cancer cell line. The immunodominance of this epitope was found not to be due to a putative HLA-DR1 epitope (p98-112) flanking p103-111. Finally, we demonstrated that SSX-2 epitope-specific CTL could be detected and cultured from the peripheral blood of HLA-A2+ prostate cancer patients, notably patients with advanced prostate cancer. Overall, we conclude that SSX-2 peptide p103-111 is an immunodominant HLA-A2-restricted epitope, and epitope-specific CD8+ T cells can be detected in patients with prostate cancer, suggesting that tolerance to SSX-2 can be circumvented in vivo. Together, these findings suggest that SSX-2 may be a relevant target antigen for prostate cancer vaccine approaches.
As the most commonly diagnosed and second leading cause of cancer-related death among American men, prostate cancer is a significant health concern with limited treatment options available for advanced stages of disease . Approximately one-third of men diagnosed will ultimately develop recurrent disease after primary surgery or radiation therapy [1, 2]. Tumor immunotherapy has become an increasingly active area of research for recurrent or metastatic prostate cancer [2, 3], and genetic vaccines encoding cDNAs for tumor antigens represent one type of immunotherapeutic vaccine delivery system being evaluated in clinical trials and showing promise [4-9].
Our group is particularly interested in identifying appropriate antigen targets for anti-prostate tumor vaccines, and vaccine strategies that are simple and capable of eliciting antigen-specific cytotoxic T lymphocytes (CTL). DNA plasmid vaccines offer several advantages over other genetic vaccine strategies by providing a safe immunization platform, simple preparation, and inexpensive manufacture, while also specifically targeting only a select antigen(s) or antigenic epitope(s) [10, 11]. Several DNA vaccines have been shown to elicit anti-tumor immune responses in patients with prostate cancer, however, the majority of antigens targeted to date have been tissue-specific and not tumor-specific [5-7, 12]. An important consideration for the development of future vaccines is to identify target antigens that are specific to tumor tissue, thereby circumventing potential cytotoxicity against normal tissues.
Cancer-testis antigens (CTA) are one class of tumor antigen not previously explored to a great extent for prostate cancer immunotherapy. These proteins are defined based upon their tissue-restricted expression to germline cells and their frequent ectopic expression in a variety of tumors [13-16]. The nearly exclusive expression of CTA in germline tissue makes them attractive therapeutic targets because germ cells do not express class I MHC molecules. Moreover, because CTA are expressed in many types of cancer, and especially advanced-stage disease, immunotherapeutic vaccines targeting these antigens may have efficacy for multiple types of malignancy in both a prophylactic and therapeutic setting.
One of the first CTA identified was HOM-MEL-40, or SSX-2, which is known to be expressed in advanced-stage tumors of different histological types [17, 18]. Moreover, SSX-2 mRNA expression has been associated with a worse prognosis in several types of cancer . We have identified this antigen as a potential target for prostate cancer based upon its ectopic expression in advanced prostate cancer, and because IgG responses specific for SSX-2 can be detected in some patients . Additionally, we have shown that SSX-2 expression can be selectively induced in prostate cancer cells upon treatment with the DNA methyltransferase inhibitor 5-aza-2’-deoxycytidine (5-aza-dc) . This may have clinical application because these epigenetic modifying agents are being evaluated as cancer treatments ; increased antigen expression with epigenetic modifiers may increase antigen processing and epitope presentation on prostate cancer cells, leading to enhanced recognition by antigen-specific CTL.
To aid in the evaluation of SSX-2 as an immunotherapeutic target, several groups have sought to identify MHC class I-restricted SSX-2 epitopes. The majority of the investigations have utilized reverse immunology approaches culturing T cells from healthy donors or cancer patients with pools of SSX-2 peptides [19, 21-25]. While these studies have successfully identified several HLA-A2-binding peptides, it is not known whether SSX-2 epitopes are elicited as a result of in vivo vaccination or whether dominant epitopes exist for this antigen. The current study was conducted to evaluate the potential of a genetic vaccine encoding SSX-2 to elicit epitope-specific immune responses in an in vivo mouse model, to more comprehensively compare SSX-2 HLA-A2-restricted T-cell epitopes, determine whether epitope-specific CTL can lyse prostate tumor cells, and evaluate whether these epitope-specific CTL exist within the T-cell repertoire of prostate cancer patients. To identify and characterize SSX-2 epitopes, we used two approaches. The first was a standard approach to determine the HLA-A2 affinity of SSX-2-derived peptides in vitro, and secondly we used a genetic vaccine to determine which peptides are processed and presented as epitopes by antigen-presenting cells (APCs). For these vaccine studies we immunized transgenic A2/DR1 mice, which are engineered to express human HLA-A2 and HLA-DR1, but not murine MHC class I and II complexes . We found that a plasmid vaccine encoding SSX-2 could elicit robust epitope-specific CTL capable of lysing prostate cancer cells. Additionally, these same epitope-specific CTL could be detected in patients with prostate cancer, particularly in patients with advanced disease, suggesting that this antigen is naturally immunogenic. These finding suggest that vaccines targeting SSX-2 might be developed to augment existing immunity, or perhaps in a prophylactic setting to prevent the development of SSX-2+ prostate cancer tumors.
HLA-A2.01/HLA-DR1-expressing, murine MHC class I/II knock-out transgenic (A2/DR1) mice on C57Bl/6 background were obtained from Charles River Labs (France) courtesy of Dr. Francois Lemonnier . Mice were maintained in microisolater cages under aseptic conditions and all experimental procedures were conducted under an IACUC-approved protocol.
SSX-2 cDNA was cloned from a testis cDNA library into the pTVG4 immunization vector  using standard molecular biology techniques. pTVG4 and pTVG-SSX-2 plasmids were purified using the Endo-free Plasmid Giga Kit for animal studies (Qiagen, Valencia, CA). Expression of SSX-2 was confirmed using transient transfection assays of Cos-7 cells (Effectene transfection reagent, Qiagen). SSX-2 mRNA expression was confirmed by reverse-transcription PCR (One-Step RT-PCR kit, Qiagen), and SSX-2 protein expression from cell lysates was confirmed by Western blot using an SSX-2-specific monoclonal antibody (clone 1A4, Abnova, Walnut, CA).
Seven nonamer peptides derived from the amino acid sequence of SSX-2 and predicted to have affinity for HLA-A2 in the SYFPEITHI and BIMAS prediction algorithms [29, 30] were synthesized, and the purity and identity of each peptide was confirmed by mass spectrometry and gas chromatography (United Biochemical Research, Seattle, WA). T2 binding assays were conducted as previously described . Peptide-HLA-A2 binding was measured as relative mean fluorescent intensity (MFI), normalized to vehicle-pulsed T2 MFI, and all measurements were conducted in triplicate.
For peptide vaccination experiments, 4-6 week-old A2/DR1 mice were immunized subcutaneously with 100μg peptide in complete Freund’s adjuvant (Sigma, St. Louis, MO). Mice were euthanized seven days later and spleens were collected, processed through a mesh screen, and splenocytes were isolated by centrifugation after red blood cell osmotic lysis with ACK lysis buffer (0.15M NH4Cl, 10mM KHCO3, 0.1mM EDTA). For DNA vaccination studies, 4-6 week-old A2/DR1 mice were immunized intradermally in the ear pinna with 100μg of either pTVG4 or pTVG-SSX-2 vaccines. Mice were immunized every 14 days. Two weeks after the sixth immunization, mice were euthanized and spleens were collected and processed as described above.
IFNγ ELISPOT was performed according to the manufacturer’s instructions (R&D Systems, Minneapolis, MN), and as previously described [6, 31]. Dried plates were counted with an automated plate reader (Autoimmun Diagnostika). The number of spots was corrected for media alone negative control, and reported as the number of IFNγ spot-forming units (SFU) per 106 splenocytes.
Splenocytes were stimulated for seven days with 2μg/ml peptide in RPMI 1640 media supplemented with 10% FCS, 50mM HEPES, 1 mM sodium pyruvate, 0.1μM β-mercaptoethanol. 10U/mL murine interleukin-2 (IL-2) (R&D Systems) was added 24 hours later. Following culture, splenocytes were collected and incubated for four hours with target cells (peptide-pulsed T2 cells, DU145 or LNCaP prostate cancer cells transfected to constitutively express HLA-A2 (gift of Dr. Lawrence Fong), or LNCaP cells previously incubated for 15 minutes with 5 μg/mL anti-human HLA-A2 blocking antibody (BD Biosciences)) at various effector to target ratios. This LNCaP cell line was demonstrated to express HLA-A2 and SSX-2. After incubation, supernatants were collected and levels of lactate dehydrogenase were measured using the CytoTox 96 Non-Radioactive Assay (Promega, Madison, WI). The percentage of peptide-specific lysis was quantified using the following equation:
Blood was obtained from HLA-A2+ male volunteer blood donors, and from patients with prostate cancer, with IRB-approved, written consent. Peripheral blood mononuclear cells (PBMCs) were prepared by density centrifugation, and cryopreserved in liquid nitrogen until use. CD8+ T cells were enriched from PBMCs by magnetic bead negative selection and were cultured in vitro with peptide-loaded and irradiated autologous dendritic cells (DCs) each week for up to 5 weeks in RPMI 1640 media supplemented with 10% human AB serum, 1 mM sodium pyruvate, 0.1μM β-mercaptoethanol, and 10U/mL human interleukin-2 (IL-2) as described previously . Cells were evaluated for target cell lysis weekly by cytotoxicity assays as described above.
Patient PBMCs or murine splenocytes were evaluated directly or stimulated in vitro for one week with 1μg/ml of peptides p41-49 or p103-111 prior to staining with APC-labeled tetramers or APC-labeled IgG κ isotype control (BD Pharmingen, San Diego, CA). Lymphocytes were gated on CD3+/CD8+ populations and tetramer-positive staining was defined as the percentage of CD8+/APC+ events among CD3+CD8+ T cells by comparison with a corresponding IgG-APC isotype control gate set to include ≤ 0.05% of CD3+CD8+APC+ events.
To evaluate potential HLA-A2-restricted SSX-2 epitopes, two peptide-binding prediction algorithms were used to scan the 188-amino acid SSX-2 protein sequence for possible HLA-A2*0201-binding peptides [29, 30]. Among the ten highest predicted HLA-A2 binding peptides identified by each algorithm, seven nonamer peptides were shared and chosen for further analysis. T2 binding assays were then carried out to rank the binding of each peptide to HLA-A2, the results of which are shown in Figure 1A. Four peptides (p41-49, p57-65, p99-107, and p103-111) were identified with binding scores comparable to a known influenza A matrix protein HLA-A2 epitope (GILGFVFTL). As shown in Figure 1B, peptides p41-49 and p103-111 were found to have the greatest avidity, stabilizing the surface expression of HLA-A2 at lower concentrations than peptides p57-65 and p99-107. The identified human peptides were also compared to the corresponding amino acids from two murine SSX orthologues (Ssxa1 and Ssxb1) to determine whether these peptides might represent highly homologous “self” antigens in a murine system and have similar HLA-A2 affinity. As shown in Table 1, the murine homologues were not highly homologous to the human peptides.
To determine whether the four identified HLA-A2-binding peptides can be presented by APCs in vivo, immunization studies were carried out in HLA-A2 transgenic A2/DR1 mice . Animals immunized once with one of the four predicted HLA-A2 binding peptides or an influenza A matrix protein peptide were tested for the frequency of peptide-specific immune responses by IFNγ ELISPOT. As shown in Figure 2, approximately half of the p103-111-immunized animals developed high frequency p103-111-specific immune responses (p = 0.041), while lower frequency SSX-2 p41-49-specific responses were observed in mice immunized with p41-49 (p = 0.039). In multiple vaccination experiments no peptide-specific immune responses to peptides p57-65 or p99-107 were detected. Animals immunized with the influenza peptide developed robust responses that were specific to this peptide but not to the p103-111 or p41-49 peptide. These results demonstrate that SSX-2 peptides p41-49 and p103-111 are immunogenic in this transgenic mouse model.
To evaluate whether these SSX-2 peptides are epitopes presented by prostate cancer cells, splenocytes from peptide-immunized animals were evaluated for lysis of an HLA-A2- and SSX-2-expressing LNCaP cell line. Splenocytes from animals immunized with peptide p41-49 or flu peptide lysed peptide-pulsed target cells but not LNCaP cells (panels A and E), while splenocytes from animals immunized with p57-65 (panel B) or p99-107 (panel C) were not lytic (Figure 3). Peptide p103-111-specific CTL were also capable of lysing LNCaP cells (panel D), and this lysis was abrogated in the presence of an HLA-A2 blocking antibody (data not shown).
As a complementary approach to characterize HLA-A2-restricted epitopes, we evaluated a genetic vaccine encoding SSX-2. We reasoned that a DNA vaccine should elicit both MHC class I and class II restricted responses by direct antigen processing and presentation by host antigen-presenting cells. SSX-2 cDNA was cloned into the pTVG4 immunization vector , and transcription and translation from the pTVG-SSX-2 construct was confirmed (Supplementary Figure 1). A2/DR1 mice immunized with pTVG-SSX-2 were found to develop robust (p=0.0012) p103-111-specific immune responses detectable directly ex vivo (Figure 4A). Additionally, significant p41-49-specific immune responses (p = 0.022) could be detected as well; these responses were of lower frequency as in vitro stimulation was necessary to detect these responses (Figure 4B). T cells specific for p103-111 could similarly be detected directly by tetramer staining; few p41-49-specific T cells could be detected without in vitro stimulation. Epitope-specific T cells were not detected in pTVG4-immunized animals (Figure 4C), and responses to other SSX-2-derived peptides were not detected in either group even following peptide stimulation in vitro. These results indicate that peptides p41-49 and p103-111 are naturally-processed, immunogenic SSX-2 epitopes. Splenocytes from DNA-immunized mice were also evaluated for SSX-2 peptide-specific CTL (Figure 5). Mice vaccinated with pTVG-SSX-2 developed p41-49 and p103-111 peptide-specific CTL (Figure 5A) that could also lyse the SSX-2+ LNCaP prostate cancer cell line, but not an HLA-A2-expressing prostate cancer cell line not expressing SSX-2 (DU-145); the CTL lysis was not detectable in the presence of an HLA-A2 blocking antibody (Figure 5B). Splenocytes from animals immunized with the pTVG4 control did not generate SSX-2-specific CTL (5C and 5D).
We next questioned why the responses elicited to peptide p103-111 were more robust than peptide p41-49, even though both had similar HLA-A2 avidity in vitro and both were being presented from the same DNA construct. We suspected this may be due to the amino acid context surrounding these epitopes. Using a peptide prediction algorithm, we identified a putative class II peptide (p98-112) that encompasses p103-111 and among all potential 15-mer peptides derived from the amino acid sequence of SSX-2 was predicted to have the highest affinity for HLA-DR1 . To evaluate this further, mice were immunized with SSX-2 peptide p98-112 or peptide p103-111 and assessed for peptide-specific immune responses. Animals immunized with peptide p98-112 developed p98-112-specific IFNγ-secreting immune responses that could be blocked with an HLA-DR blocking antibody, but interestingly, no HLA-A2-restricted response was generated. In contrast, all animals immunized with p103-111 developed potent p103-111-specific immune responses that could be blocked with an HLA-A2 blocking antibody (Figure 6A). Similarly, A2/DR1 mice (n=8) immunized with pTVG-SSX-2 were tested for p98-112 peptide-specific immune responses. While the DNA vaccine elicited robust p103-111-specific immune responses that could be blocked with an HLA-A2 blocking antibody, no significant p98-112-specific immune responses were detectable (Figure 6B). These results indicate that while p98-112 is an immunogenic HLA-DR1 peptide, it may not necessarily serve as an epitope processed and presented by APCs, and did not facilitate recognition of the p103-111 epitope.
We next wished to determine whether SSX-2 epitope-specific T cells are detectable in the peripheral blood of patients with prostate cancer, as evidence of whether these cells are present within the repertoire of HLA-A2+ individuals and thus might be able to be augmented with immunization. PBMC were obtained from volunteer male HLA-A2+ blood donors without prostate cancer (n=6), HLA-A2+ (n=7) or HLA-A2- (n=6) men with biochemically recurrent prostate cancer, and HLA-A2+ men (n=8) with advanced, castrate-resistant prostate cancer. These PBMC were stimulated for 1 week in vitro with peptides p41-49 and p103-111 and evaluated for the presence of peptide-specific T cells by tetramer staining. As shown in Figures 7A and 7B, SSX-2-specific CD8+ T cells were detectable at a significantly higher frequency in patients with prostate cancer compared with volunteer HLA-A2+ male blood donors. Moreover, the frequency of SSX-2-specific CD8+ T cells was generally higher in patients with more advanced disease. CD8+ T cells were also isolated from the peripheral blood of eleven HLA-A2+ patients with prostate cancer and tested for their ability to lyse SSX-2 p103-111 peptide-pulsed target cells. CTL specific for peptide-pulsed target cells were detectable from one of seven HLA-A2+ patients with biochemically recurrent prostate cancer after five in vitro stimulations, and were similarly detectable from three of four HLA-A2+ patients with castrate-resistant prostate cancer following two-to-three stimulations in vitro (Figure 7B and 7C). All patients with T cells showing demonstrable lysis were found to have demonstrable p103-111 tetramer-specific T cells (data not shown). In one patient with a high frequency of p103-111 peptide-specific T cells after in vitro stimulation found by tetramer staining (0.85% of CD3+/CD8+ splenocytes), peptide-specific CTL could be detected after three in vitro stimulations with peptide and could also lyse the LNCaP prostate cancer cell line (Figure 7C).
Several vaccines designed to elicit antigen-specific immune responses are currently being evaluated in clinical trials as specific, targeted treatments for patients with prostate cancer. Because the prostate is an expendable organ, most immunotherapy studies have targeted tissue-specific antigens. For example, vaccines targeting prostate tumor-associated antigens such as prostate-specific antigen (PSA) and prostatic acid phosphatase (PAP) have been shown to elicit antigen-specific immune responses in patients with prostate cancer [5, 6, 12, 32-35]. However, PSA and PAP are not tumor-specific proteins; the identification of tumor-specific targets remains important to the development of immunotherapeutic treatments for prostate cancer, as well as other types of malignancy. Recently, Cheever et. al. reported that SSX-2 and other sarcoma translocation breakpoint antigens may be higher priority targets for cancer therapy than PSA or PAP based upon certain pre-defined criteria for the prioritization of tumor antigens, such as specificity, oncogenicity, expression in multiple tumor types or advanced disease, and number of identified epitopes . Since the majority of prostate cancer antigens targeted therapeutically to date have been tissue-specific and not necessarily tumor-specific, and because the evaluation of targets associated with more advanced disease may have relevance to prostate cancer, we sought to further characterize the immunogenicity of SSX-2. In addition, because this cancer-testis antigen is not normally expressed in MHC class I-expressing cells, unlike the tissue-restricted antigens currently being investigated for prostate cancer, we reasoned that it might be possible to evaluate SSX-2 in the future as a prophylactic cancer vaccine antigen, ideally permitting immunization in a setting prior to expression of the antigen in tumor cells and thereby potentially avoiding immune tolerance.
In the present study we used a genetic vaccine to identify and compare SSX-2 HLA-A2-restricted epitopes relevant to prostate cancer. Specifically, we used peptide prediction algorithms and HLA-A2 affinity assays to identify and prioritize potentially immunogenic peptides, followed by in vivo vaccination with SSX-2 peptides or a DNA plasmid encoding SSX-2 to evaluate the immunogenicity of predicted peptides and their ability to elicit anti-tumor CTL. With this approach we identified four SSX-2 peptides that have significant affinity for the HLA-A2 complex in vitro. We subsequently carried out peptide vaccination studies in A2/DR1 mice and found that p41-49 and p103-111 could elicit peptide-specific immune responses in this transgenic model. Using a genetic vaccine encoding SSX-2 we found that significant responses to p41-49 and p103-111 were elicited, demonstrating that both peptides are HLA-A2-restricted epitopes. However, peptide p103-111 appeared to be clearly dominant with robust responses elicited by either direct peptide or DNA vaccination. p103-111 immunization elicited CTL in animals that could lyse both p103-111 peptide-pulsed target cells and LNCaP prostate cancer cells. The dominance of the p103-111 epitope was found to not be due to a putative MHC class II epitope encompassing it. Finally, we also found that HLA-A2+ patients with prostate cancer can have p41-49 and p103-111-specific T cells in their peripheral blood, demonstrating that these cells are within the repertoire of patients with advanced disease in particular.
Several other investigators have also sought to identify MHC class I-restricted epitopes specific for SSX-2. Ayyoub et. al. previously evaluated SSX-2 peptides by incubating an overlapping library of SSX-2 peptides with standard proteasome complexes in vitro . They demonstrated that peptide p41-49 is an HLA-A2-restricted epitope recognized by CTL from melanoma patients . Peptide p41-49 was also shown to be an epitope presented in hepatocellular carcinoma, and p41-49-specific CTL were found to lyse melanoma cells and the sarcoma cell line SW 872 [25, 37-39]. Wagner et. al. independently demonstrated that SSX-2 peptide p103-111 is an HLA-A2-restricted epitope recognized by CTL from breast cancer patients and presented by melanoma cell lines . Held et al. further demonstrated that this epitope is directly presented on melanoma cells using peptide/HLA-A2-specific Fab antibodies . He et al. suggested that SSX-2 p57-65 was also an HLA-A2-restricted epitope by demonstrating that peptide-specific CTL cultured from healthy donors are capable of lysing peptide-pulsed T2 cells , however, they did not demonstrate that this peptide is presented as an MHC class I-restricted epitope by tumor cells. Together, these prior studies highlighted these three SSX-2 peptides as possible HLA-A2-restricted epitopes. Prior to our study, a comparison of HLA-A2-restricted epitopes had not been carried out, and it had not been determined whether SSX-2 vaccines can elicit epitope-specific CTL to these peptides. Our studies confirm the work of others that p41-49 and p103-111 are HLA-A2-restricted epitopes. However, while p57-65 can bind HLA-A2, it does not appear to be a presented HLA-A2-restricted epitope, and it does not appear to be immunogenic in our transgenic model.
We found that in vivo immunization of transgenic mice with peptide p103-111 or a DNA vaccine encoding SSX-2 generated robust p103-111-specific immune responses that could be detected directly ex vivo. Peptide p41-49-specific immune responses generated by peptide or genetic immunization were only rarely detected unless the splenocytes were expanded by in vitro peptide stimulation (Figure 4B and 4C). These results were not expected from our in vitro analysis, which showed p41-49 to have the greatest affinity for HLA-A2. It could be that p41-49 is not efficiently processed by the murine proteasome. Interestingly, the Ssxa1 murine peptide corresponding to human peptide p41-49 shares the greatest homology with the human peptide, while the region corresponding to p103-111 is absent in the mouse protein sequence (Table 1). Consequently, it is possible that there is some tolerance to the p41-49 epitope in the mouse that contributed to the reduced immunogenicity observed. Peptide p41-49-specific CTL from DNA-immunized mice were able to lyse p41-49-pulsed target cells, suggesting that p41-49 is an HLA-A2-restricted epitope, yet in our studies p41-49-specific CTL failed to lyse SSX-2+ LNCaP cells (Figure 3A). These results were also unexpected but may indicate that LNCaP cells either do not endogenously process and present this peptide, present low levels of this peptide, or perhaps more likely, p41-49-specific CTL generated from vaccination are too low in frequency or avidity to detect this epitope on LNCaP cells. In any case, our results suggest that p103-111 is the dominant HLA-A2-restricted SSX-2 epitope, at least in this transgenic mouse model.
We found that genetic vaccination offered several advantages over direct peptide immunization for epitope identification, including the induction of stronger immune responses and simultaneous recognition of multiple epitopes. Immunization of mice with pTVG-SSX-2 elicited p103-111-specific immune responses in every immunized animal and with higher frequency than p41-49-specific cells as determined by both IFNγ ELISPOT and tetramer staining of splenocytes directly ex vivo. Interestingly, the best in vitro lysis of LNCaP cells and peptide-pulsed T2 cells (figure 5A and 5B) was found by splenocytes from an animal that had a robust immune response to both peptides p41-49 and p103-111 by direct ex vivo IFNγ ELISPOT assay (data not shown). This finding may indicate that superior lysis of prostate tumor cells can be achieved by eliciting CTL specific for multiple SSX-2 epitopes. A possible future direction may be to develop the SSX-2 DNA vaccine as an immunotherapeutic tool by optimizing its ability to elicit responses to multiple epitopes simultaneously.
In addition to identifying SSX-2 peptide p103-111 as an immunodominant epitope, we were able to detect T cells specific to this peptide in the peripheral blood of patients with prostate cancer using tetramer staining, with particularly high frequencies of T cells specific to either peptide p41-49 or p103-111 in patients with advanced disease. In concordance with these tetramer results we found that one of seven patients with early-stage prostate cancer had CTL that could specifically lyse p103-111-pulsed target cells, while three of four patients with advanced-stage disease had higher frequencies of tetramer positive CTL that could lyse these target cells or the LNCaP prostate cancer cell line after fewer rounds of in vitro peptide stimulation. These findings suggest that advanced prostate tumors might have greater SSX-2 expression than earlier stage disease, as has been suggested by findings of increased SSX-2 mRNA expression in metastatic prostate cancer and other tumor types [18, 42], which could lead to increased cross-presentation of SSX-2 and generation of SSX-2-specific CD8+ T cells. If true, this might permit an opportunity to consider immunization early in the course of disease, more analogous to the prophylactic setting, to ideally prevent the growth of SSX-2-expressing tumors. However, to date the expression and function of SSX-2 in prostate tumors of various stages remains largely unknown. These will be areas of future research.
In conclusion, this work provides direct evidence that SSX-2 is a relevant vaccine target antigen for prostate cancer. In particular these findings highlight the importance of SSX-2 peptide p103-111 as a dominant HLA-A2-restricted immunogenic epitope. To date the majority of the work evaluating SSX-2 has focused on SSX-2 peptide p41-49 as an HLA-A2-restricted epitope in patients with different types of malignancy. Future studies will explore the expression and function of SSX-2, and other SSX family members, in prostate tumors. Other studies will explore modified DNA vaccines as a means to enhance peptide-specific immune responses to this antigen and will assess strategies to augment the anti-tumor potential of these SSX-2-specific vaccines in vivo, such as using these vaccines in combination with epigenetic modifying agents that might increase SSX-2 expression in tumor cells (18).
Full-length SSX-2 cDNA was cloned into the pTVG4 immunization vector to construct the DNA vaccine (panel A). To confirm that SSX-2 is expressed from the DNA plasmid when taken up by host cells, transient transfection assays were carried out in which Cos-7 cells were transfected with either pTVG-SSX-2 or the empty pTVG4 plasmid. RNA and protein samples were collected at 24 and 48 hours post-transfection to test for SSX-2 expression via RT-PCR (panel B) and Western blot (panel C). Expected molecular weight of SSX-2 protein (approximately 21 kDa) is indicated by the arrow.
This work was supported for HAS by NIH (T32CA009135-33) and for HAS and DGM by the US Army Medical Research and Materiel Command Prostate Cancer Research Program (W81XWH-08-1-0341 and W81XWH-10-1-0495). We also thank Dr. Francois Lemonnier for his provision of the A2/DR1 mice, Dr. Brian Olson for providing technical support and reviewing the manuscript, and the NIH Tetramer facility (Atlanta, GA) for providing the HLA-A2 tetramers.
Disclosures: All authors have declared there are no financial conflicts of interest in regards to this work.