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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Endocrinol Metab Clin North Am. Author manuscript; available in PMC 2012 September 1.
Published in final edited form as:
PMCID: PMC3167093
NIHMSID: NIHMS299266

Estrogens and Prostate Cancer: Etiology, Mediators, Prevention, and Management

Shuk-Mei Ho, Ph.D., Ming-tsung Lee, M.Phil., Hung-Ming Lam, Ph.D., and Yuet-Kin Leung, Ph.D.

Abstract

The relationship between hormones and the pathogenesis of prostate cancer (PCa) has been studied extensively. All the mainstay targets for hormonal PCa therapies are based on negating androgen action. Recent epidemiologic and experimental data have clearly pinpointed the key roles of estrogens in PCa development and progression. Racial and geographical differences, as well as age-associated changes, in estrogen synthesis and metabolism contribute significantly to the etiology by increasing the ratio of circulating estrogen to androgen, sex hormone binding globulin synthesis, and aromatase activity and reducing androgen glucuronidation and tissue bioactivation. Promotion of aberrant cell growth, evasion of apoptosis, increased oxidative stress and inflammation, and gains in adiposity and bioactivation to genotoxic carcinogens during adulthood are probable mechanisms of estrogen carcinogenicity, while “estrogen imprinting” via epigenetics in early-life also determines PCa risk. Although the effects of estrogens are known to be mediated by genomic actions of the two estrogen receptor (ER) subtypes (ERα and ERβ), other non-canonical mediators, including the different ERβ isoforms, membrane and mitochondrial ERs, and G protein-coupled receptor 30, may have major actions diverging from classical ER actions. These new discoveries have led to renewed interest among the public and the medicinal field in estrogens and antiestrogens as singular and adjuvant PCa treatment and prevention regimens. This review summarizes current knowledge on how different estrogens/antiestrogens/estrogen mimics contribute to prostate carcinogenesis, the roles of the different mediators of estrogen in the process, and the potentials of new estrogenic/antiestrogenic compounds as targeted therapies for prevention and treatment of PCa.

Keywords: Racial disparity, aging, sex steroid–binding globulin, estrogen receptor, ER spliced variants, GPR30, aromatase, antiestrogens, selective estrogen receptor modulator (SERM), nuclear receptor co-regulators, DNA methylation, developmental reprogramming, apigenin, phytoestrogen, apoptosis, oxidative stress, genomic damages, prostate cancer risk, hormonal therapy, epigenetics, genistein, resveratrol, raloxifene, toremifene, fulvestrant, estramustine, catecholestrogen

Androgens are traditionally recognized as the major hormone promoting normal and aberrant growth of the prostate. Recent literature, however, suggests that estrogen could also be an important mediator of these processes. Estrogen alone or in synergy with androgen is responsible for the pathogenesis of prostate cancer (PCa). More important, recent experimental data suggest that estrogen or its mimics could determine the risk of PCa development as early as the prenatal stage via a process known as “estrogen imprinting.” Here we a) review research findings that support a role for estrogens, estrogen mimics and estrogen metabolites in prostate carcinogenesis; b) discuss how different estrogen receptors (ER) mediate the action of estrogen in promoting the development and progression of PCa; and c) evaluate the potentials of estrogens, xenoestrogens, phytoestrogens, antiestrogens, and SERMs as therapeutics for prevention and treatment of PCa.

Epidemiologic and Animal-model Studies of the Relationship between Estrogens and Pathogenesis of PCa

Results from epidemiologic studies have suggested a role for estrogen in the pathogenesis of PCa. Racial/ethnic and geographical differences in the levels of estrogens provide a probable explanation for the disparity in the prevalence of PCa among various populations throughout the world.1-3 Apropos to this view is the finding that levels of circulating estrogens in African-American men, whose incidence of PCa is the highest in United States, are higher than those in Caucasian Americans throughout their adult life.4-8 In contrast, Japanese men, whose incidence of PCa is low, have lower circulating levels of estrogen than do Dutch-European men.9 A global study on 5,003 men aged 65 years or older showed that blacks (in the US and in West Africa) had higher estrogen levels than Caucasians or Asians (in the US and in their homelands), with levels of total and free estradiol-17β (E2) 10–16% higher and levels of estrone (E1) 27–39% higher than in the latter group.10 Moreover, the ratios of total E2 to total testosterone (T) and E1 to androstenedione were higher in blacks than in the other groups.10 A comprehensive analysis of the levels of androgens, estrogens, and their metabolites in circulation led to the conclusion that two fundamental metabolic processes, increased aromatase activity and reduced androgen glucuronidation, are major factors governing the ratio of estrogen to androgen in elderly men.10

Age is also a key risk factor for PCa.1 The prevalence of PCa increases dramatically as men age; this is paralleled by a significant increase in the ratio of circulating estrogen to androgen levels, which may increase by up to 40%.11-17 This age-related hormonal change, often referred to as “andropause,” is caused by several endocrine events, including a decline in testicular function, and increases in adiposity, extragonadal aromatization, and the production of sex hormone–binding globulin (SHBG) as men age.12;18-21 The level of 5α-dihydrotestosterone (DHT) was found to decrease whereas those of estrogen (both E2 and E1) in the epithelial cells, to increase in the aging prostate (Figure 1).22 Since estrogens can be synthesized de novo via aromatase activity in the prostate,23 tissue estrogen levels may be more important than circulating estrogen levels in promoting prostate carcinogenesis and progression. In this regard, recent studies utilizing laser capture microdissection samples demonstrated that stromal rather than epithelial aromatase activity may be important in upregulating the E2:T ratio in the tumor site via an alternative promoter activation mechanism during prostate carcinogenesis.24;25 Furthermore, aromatase-knockout mice, which cannot produce E2 locally in the prostate, have elevated levels of circulating T and DHT and, with age, are prone to the development of benign prostatic hyperplasia (BPH) but not PCa.26 Collectively, increased estrogenic influences on the prostate due to racial differences or andropause27 may elevate the risk of neoplastic transformation of the prostatic epithelium in men.3;28;29

Figure. 1
Expression of various hormone receptors and levels of hormones during the development and progression of prostate cancer (PCa). In the normal human prostate (far left panel), androgen receptor (AR), estrogen receptor alpha (ERα), different estrogen ...

Experimental models also support the suggestion that estrogens, alone or synergistically with androgens, are potent inducers of aberrant growth and neoplastic transformation in the prostate.1;2;30-33 Prenatal exposure to maternal estrogens or adult exposure to pharmacologic doses of estrogens induces a benign lesion termed squamous metaplasia, which is derived from the basal-cell proliferation of the prostates of various species, including humans.34 In a susceptible rat strain (Noble rats), chronic exposure to T plus E2 in adulthood promoted the evolution of a precancerous lesion similar to human prostatic intraepithelial neoplasia (PIN) and a high incidence of full-blown PCa31;35-37 via the alteration of levels of estrogen to androgen in a manner mimicking that in aging men.35-38 Paradoxically, dietary soy that is rich in phytoestrogens can mitigate the tumor-promoting effect of T plus E2 in this rat model.39 Moreover, treatment of the nude mice with T plus E2 could promote the progression of PCa and metastasis at distant organs in the tissue recombinants composed of mouse mensenchyme and a human prostatic epithelial cell line.40 Cellular and molecular changes implicated as the mechanisms leading to prostate carcinogenesis include a) a dramatic increase in the proliferation of epithelial cells;37 b) upregulation of growth factor–signaling pathways (e.g. TGFα/EGF receptor signaling;41 TGFβ signaling,42 IGF-1 and VEGF signaling,43 and ER signaling);44 c) prolactinemia or increased prolactin-receptor signaling;45 d) mitogen-activated protein kinase (MAPK) activation, possibly through Id-1;46;47 e) increased cell-survival potential through the overexpression of anti-apoptotic mediators (e.g., metallothionein48 and TRPM-2/clusterin);49;50 f) elevation in oxidative stress-induced DNA damage;51-53 g) changes in gene-expression profiles related to cell proliferation, DNA damage, activation of proto-oncogenes and transforming factors, IL-1B signaling, and TNF-α activation;54;55 and h) breakdown of epithelial basement membrane and stromal extracellular matrix due to the increase in gelatinolytic proteinase activity and altered expression of glycoconjugates in smooth muscles and their associated extracellular matrix.56-58 Another potential culprit associated with estrogen-induced/promoted PCa is hormone-induced chronic inflammation,38;59-61although this view is not uniformly supported by all studies.62

Influence of Estrogen Bioactivation and Detoxification on PCa Risk

The carcinogenic action of estrogen could be caused, in part, by metabolic activation of the natural estrogens E1 and E2 to genotoxic metabolites, such as 2- and 4-hydroxyl catechol estrogens, and their quinone/semiquinone intermediates that act as chemical carcinogens.63 The bioactivation process is mediated principally by two cytochrome P450 enzymes, CYP1A1 and CYP1B1, whereas the enzyme catechol-O-methyltransferase (COMT) is responsible for the inactivation and removal of these genotoxic estrogen-derived intermediates.63-66 Once formed, these genotoxic metabolites can cause genomic damages through processes such as the formation of DNA adducts.67 Supporting the suggestion that the genotoxicity of estrogen metabolites is involved in prostate carcinogenesis is the observation of increased DNA strand breakage52 and nuclear staining of 8-hydroxy-2′-deoxy-guanosine60 in the prostates of rats treated with T plus E2. Conversely, increased expression or activity of COMT was shown to protect against estrogen-induced cancer by mediating the conversion of catechol estrogens into methoxyestrogens that have potent apoptotic activity against rapidly growing PCa cells,68 prompting the testing of combinatory therapies involving methoxyestrogens and other standard therapies, such as hormone deprivation,69 docetaxel,70 and eugenol71 against PCa growth in model systems.

Furthermore, the aforementioned estrogen metabolites and their intermediates induce oxidative stress and likely promote the generation of high levels of reactive oxygen species (ROS), with damaging effects on proteins, lipids, and DNA in target tissues.29;66 Therefore, several isoforms of glutathione S-transferases (GSTs) have been reported to have protective effects against estrogen genotoxicity through the detoxification of ROS. Genetic polymorphisms that alter enzyme activity and epigenetics- or mutation-mediated silencing of the cognate genes are expected to affect the PCa risk by modulating the extent of lifelong exposure to genotoxic estrogen metabolites.29;72 Thus, polymorphisms of the CYP1A1m1 allele, the CYP1B1-Leu432Val, COMT at codon 62 and 158, and the GSTM1-null genotype have been shown to modify PCa risk in certain populations.73-80 In addition, a CYP1A1 variant (CYP1A1v) that resides preferentially in the nucleus and mitochondria was found to confer higher carcinogenic potential than its wild-type cytosolic counterpart.81 This finding is in agreement with the hypothesis that genotoxic estrogen metabolites produced in nuclei are potent tumor initiators.

Another metabolic pathway of relevance to estrogen carcinogenicity in the prostate is the in situ production of estrogen from androgen via aromatase encoded by CYP19.25 The enzyme and its activity have been demonstrated in the specimens of PCa and BPH.25;82 Clinical trials designed to test the efficacy of aromatase inhibitors in treating BPH83-85 and PCa86-89 have been reported. Polymorphisms in CYP19 including intron 4[TTTA]n repeat90 and C/T versus T/T genotype91 are associated with familial PCa. Continued investigation of the use of aromatase inhibitors as monotherapies or adjuvants for the treatment of PCa is warranted.26;92

Determination of PCa Risk during Early Life

The risk of developing PCa as an adult could be determined by early-life exposure to natural or environmental estrogens through a mechanism known as “estrogen imprinting.”93 Perinatal and neonatal exposure of rats94-97 or mice98-100 to estrogens or estrogen mimics induces inflammation, permanent changes in the levels of androgen and estrogen receptors, stromal hypertrophy, and elevated proliferative potentials in the prostatic epithelium of the adult aged gland.96;101-103 If the “estrogenized” adult glands are exposed to an elevated estrogen challenge during adult life (the “second hit”), full-blown PIN develops in the affected glands.104 An environmental estrogen, bisphenol A (BPA), is equally effective in sensitizing the adult prostate to increased estrogenic influence during adulthood with regard to the induction of PIN. An unbiased screening has identified permanent alterations in the methylation status of a CpG island in the promoter of phosphodiesterase 4D4 (Pde4e4), an enzyme responsible for regulating cellular cAMP.104 This finding suggests estrogen imprinting may involve epigenetic reprogramming of prostatic transcription programs in early life. Whether analogous phenomena exist in humans is unclear. However, circulating E2 levels have been found to be higher in pregnant African-American women than in pregnant Caucasian-American women.105;106 These data are consistent with the hypothesis that exposure to higher levels of E2 in utero may explain some of the differences in PCa risk among ethnic groups. Moreover, some indicators of high levels of estrogen during pregnancy, such as high birth weight and jaundice in the newborn, are associated with increased risk of PCa, whereas indicators of low estrogen levels, such as pre-eclampsia, are related to decreased risk.107;108 The male offspring of women who took diethylstilbestrol (DES) during pregnancy may have a higher risk of PCa.109 These data, taken together, indicate that PCa may be considered a fetus-based disease. In addition, the window of susceptibility for early-life reprogramming may extend to perinatal and peripubertal periods and exposures to environmental estrogens may have the same impact as natural estrogens. Of relevance to these hypotheses are studies reporting significant exposure of human fetuses to BPA, likely caused by maternal use of BPA-containing products.110-116 Perinatal exposure to BPA has also been documented.117-119 Paradoxically, concerns about developmental reprogramming of PCa risk by soy infant formulas containing high concentrations of phytoestrogens have seldom been raised.120-122 Yet, the results of numerous human studies examining the risks and benefits of adult consumption of soy and phytoestrogens for prevention of PCa have been inconclusive.123-127 In summary, if PCa risk can be reprogrammed in early life, cancer prevention strategies should be directed at the aforementioned early developmental stages.

Estrogen Receptors (ERα and ERβ) as Functionally Divergent Mediators of Estrogen Action in the Prostate

The actions of estrogens are now believed to be mediated primarily by two ER subtypes, ERα and ERβ and their variant forms.28;32;128;129 Both of the ERs have six common domains (A-F). They share a highly homologous DNA-binding domain (97% amino acid homology) but have very dissimilar N- and C-termini.130 In addition, their ligand-binding domains are 56% homologous. These structural differences are the basis of the reported significant functional differences between the two receptor subtypes. Furthermore, it has been reported that the binding of the two receptors to the same ligand can initiate recruitments of different co-regulators and trigger the utilization of different cis-regulatory elements, thus further increasing the functional diversity of these two receptors.130-135 Finally, the recent discovery of variant forms of ERα and ERβ (isoforms) caused by alternative splicing or mutations has added complexity to estrogen action because these variants clearly show distinct functional disparity and patterns of tissue-/cell type-specific distribution.129;132;136;137

Many reports have shown differential expression patterns of the two receptors in the epithelial and stromal compartments of the normal and malignant human prostate (Figure 1).33;137-144 In the normal human prostate, the wild-type ERβ (also known as ERβ1) is localized mainly in the basal epithelial compartment, where ERα is almost never found, while ERα, along with ERβ, is expressed in the stroma.141 Both ER subtypes are absent in the luminal epithelial compartment.141 A gradual loss of ERβ1 expression is apparent during the progression to high-grade PCa in the primary site 115;139;140;145;146 The progressive silencing of ERβ is accompanied by hypermethylation of a CpG island in the proximal promoter of the gene.147 Paradoxically, ERβ, but not ERα, is expressed at high levels in PCa metastasized to the bone and regional lymph nodes,141 with a concomitant loss of methylation in the CpG island of ERβ.147 Fine mapping of the CpG island revealed that this reversible silencing and reactivation of ERβ expression is attributable to cytosine methylation of an AP2 site within the CpG island of the ERβ promoter.148 Moreover, antiestrogen-based therapies involving DES or PC-SPES lowered the level of ERβ1 expression in bone metastases of the treated patients.149

It is now established that ERβ1 exerts an antiproliferative effect on the prostate epithelia131;133 and inhibits epithelial-mesenchymal transition.150 However, less is known about the biological roles of its spliced variants. We recently demonstrated that ERβ2 and ERβ5 promote metastasis and that their expression is associated with shorter metastasis-free survival in patients with PCa.137 In support of a divergent functional role of various ERβ isoforms, when a pan-ERβ antibody was used, the retention of ERβ expression in primary PCa was associated with increased mortality.139 The co-expression of ERβ with endothelial nitric oxide synthase (eNOS) and hypoxia-inducible factor 2α (HIF-2α) suggests that an estrogen-mediated NO-enriched environment may influence the aggressive phenotype of PCa significantly.151 Thus, ERβ appears to play differential roles in human prostate carcinogenesis through differential expression of the various spliced variants and possibly alternative promoter utilization.152 Differential binding of the receptor to different ligands and crosstalk with other transcriptional factor-signaling cascades may also introduce additional divergence in ERβ action.131;153;154

Studies in animal models have provided strong evidence of the antiproliferative role of ERβ in the prostate gland. ERβ knockout mice have been found to develop prostatic hyperplasia in old age, a phenomenon not seen in ERα knockout mice.155 Jiang et al.156 observed an age-dependent decline in ERβ expression in the canine prostate. Chang and Prins157 reported that neonatal exposure of rats to estrogen causes the downregulation of ERβ; the upregulation of ERα; and the development of hyperplastic, dysplastic, and neoplastic lesions in the adult ventral prostates. Consistent with the hypothesis of the antiproliferative role ERβ, Risbriger et al. demonstrated that the administration of ERβ agonists, but not of an ERα agonist, to aromatase knockout mice suppressed the prostate epithelial cell growth and promoted apoptosis158 and that the action of the receptor is mediated via TNF-α signaling.133

Apart from ERβ expression, ERα, which is expressed primarily in the stromal compartment of the prostate, may contribute to the pathogenesis of PCa. Higher levels of ERα were observed in the prostatic stroma of Hispanic and Asian men than in that of Caucasian and African-American men, who are at a higher risk for PCa.159 In a genotyping and allelic frequency analysis of six different polymorphic loci of ERα in a Japanese population, polymorphism in codon 10 was found to be associated with a higher PCa risk.160 These findings are in stark contrast to results from studies of genetic polymorphisms of ERβ161-164 that reported no strong association of various polymorphic loci with PCa risk, with the exception of one promoter single-nucleotide polymorphism (SNP).165;166

Finally, during fetal development of the human prostate, ERβ is the first to appear in the prostate (by the seventh week of gestation) and is the only ER subtype expressed in the epithelial and stromal cells during the early ductal morphogenesis.34;167 ERβ expression begins by week 15 of gestation and is strongly associated with the squamous metaplasia in the distal periurethral ducts and utricle.167 These findings suggested that ERβ, perhaps in concert with the AR, mediates the very early stage of fetal prostate development, followed by the action of ERα. Thus, selective ERβ-activating compounds may play an important role in prenatal estrogen imprinting of PCa risk.

Non-canonical Actions of Estrogen and a New Therapeutic Target (G Protein-Coupled Receptor 30)

Apart from acting as transcription factors, ERα and ERβ can trigger rapid non-genomic signal transduction at the cell membrane level through the activation of specific kinase activity or induction of a calcium influx.168-170 These membrane ER-mediated events can activate gene-transcription activities with or without synergy with the classical genomic actions of nuclear ERs.171 In this regard, membrane ERα has been shown to tether onto the epidermal growth factor receptor (EGFR) signaling pathway for the activation of the MAPK172 and phosphoinositide 3-kinase (PI3K) signaling.173 In addition, ERβ found localized in the mitochondria has been demonstrated to act as a mitochondrial transcription factor that regulates mitochondrial gene expression.174 ERβ has been reported to move from the mitochondria to the nucleus during neoplastic transformation.175 However, the functions of cell membrane or mitochondrial ERα and ERβ in the prostate and PCa remain unknown and need further elucidation.

Estrogen has recently been found to be able to exert its action via the G protein-coupled receptor 30 (GPR30).176 GPR30 has been localized in the cell membrane and endoplasmic recticulum of various estrogen-sensitive tissues and cell lines176-178 and to mediate the non-genomic action of E2 in breast, endometrial, and ovarian cancer cells through the activation of Erk1/2 and cAMP pathways.179;180 GPR30 is expressed in the immortalized normal prostate stroma cell line WPWY-1 but does not appear to mediate the growth response induced by high-dose E2.181 A recent study demonstrated positive staining of GPR30 in both plasma membrane and cytoplasm in the normal and cancerous prostate, and its predominant expression in the luminal epithelium.182 Activation of GPR30 by a non-estrogenic, synthetic, GPR30-specific ligand G-1 inhibited PCa cell growth in vitro by activating Erk1/2 and upregulating p21-mediated G2-M arrest and attenuated the growth of human PCa xenografts in nude mice.183 These new findings suggest that GPR30 is a novel estrogen mediator in the prostate and that a specific GPR30 ligand such as G-1 may have therapeutic potential for PCa.

Estrogen-related receptors (ERRs) are orphan nuclear receptors that are highly homologous to ERs, especially in the DNA- and ligand-binding domains.184 However, they do not bind natural estrogens and may have yet-to-be discovered endogenous ligands. There are at least three EERs, namely ERR-α, -β,185 and -γ.186;187 They regulate gene expression through transactivation via cis-elements such as the ERE, steroidogenic factor-1 response element (SFRE), and estrogen-related receptor response element (ERRE).188-192 Similar to ERs, different ERRs have distinct differential expression patterns in normal and cancerous prostate tissues/cells.193 Finally, although they do not bind natural estrogens, ERRβ and ERRγ do bind DES, tamoxifen, 4-hydroxytamoxifen, flavones, and isoflavone phytoestrogens194-196 and therefore should be considered in devising estrogen- or antiestrogen-based therapies for PCa.

Prevention of and Therapy for PCa with Selective ER Modulators (SERMs), Aromatase Inhibitors, Phytoestrogens, and Other Estrogen-/Antiestrogen-based Treatments

The first effective drug therapy for PCa was found about 70 years ago. DES, a xenoestrogen, was applied for the treatment of metastatic PCa197 but fell out of favor because of its cardiovascular toxicity and other serious adverse effects.33;198;199 Other ER-based treatments for PCa include antiestrogens, selective ER modulators (SERMs), and aromatase inhibitors. SERMs are estrogenic compounds that act as either ER agonists or antagonists according to the presence of different co-regulators in a cell-/tissue-specific manner. Consequently, the ERs can be either stimulated or inhibited in the cell/tissue.200 However, clinical trials have shown that different generations of SERMs, such as tamoxifen, toremifene, raloxifene, and fulvestrant, have limited efficacy as alternatives to DES.33;201-207 Their lack of efficacy could be due to their original design for targeting the transactivation of ERα on an ERE and/or blocking the traditional genomic action of estrogen. With our growing knowledge of the wide range of genomic and non-genomic actions of estrogen and the variable expression of ERβ and its isoforms in the prostate and PCa, as well as of the significance of the non-canonical ER, GPR30, the future design of estrogen/antiestrogen therapies and chemopreventive agents will have to take into account the complex nature of estrogen action in the development and progression of PCa.

In this regard, it is important to recognize that many specificities or off-target effects of estrogenic/antiestrogenic drugs are due to their broad spectrum of activities not restricted to their estrogenicity. Traditionally, estrogen-induced PCa regression is believed to be mediated by its action on the hypothalamic-pituitary axis, thereby inhibiting testosterone synthesis.208 However, it is now known that many estrogens/antiestrogens/phytoestrogens/SERMs including DES, 2-methoxy-E2, genistein, resveratrol, licochalcone, raloxifene, toremifene, fulvestrant, and estramustine, have antitumor effects independent of this pathway.209-225 The ability of these compounds to suppress PCa cell growth has been attributed to a broad range of actions, including direct cytotoxicity,223 interruption of cell-cycle progression,210;221;226 induction of apoptosis,211;221;224;225 depolymerization of microtubules,209 inhibition of DNA synthesis,213 inhibition of topoisomerase II,217 blockade of tyrosine kinase,217;218 disruption of apoptotic regulators,222 and activation of death-domain receptors.214;219 Some of these estrogenic/antiestrogenic compounds are also potent inhibitors of angiogenesis and metastasis, through their actions in upregulating expression of genes related to anti-angiogenesis or anti-metastasis,215 activating the cell adhesion–signaling molecule focal adhesion kinase,216 and reduction in metastatic spreading via the lymphatic system.220

As a recent development in the treatment of PCa, the application of estrogen patches has grown in popularity.227-229 The advantages include reduction in cardiovascular toxicity and the maintenance of adequate hormonal levels for a convenient time period.230 as well as the additional advantage of allevating hot flashes and improving bone density after endocrine treatment of PCa. 231;232 Estrogen patches have recently been tested in a phase II clinical trial as a first-line hormonal therapy in patients with locally advanced or metastatic PCa. Preliminary results produced the levels of testosterone and a PSA response similar to those of castration.228

Aside from treating PCa, the potential of SERMs for preventing PCa has turned out to be a promising proposition. The rationale stems from several lines of investigation. SERMS were shown to decrease testosterone levels by suppressing the hypothalamic-pituitary axis.233 Furthermore, age-related increases in PCa prevalence parallel increases in serum estrogen levels, and the incidence of PCa is low in countries with diets rich in phytoestrogens.234 A multicentered, double-blind randomized study involving 514 men with high-grade PIN and no cancer reported a significant reduction in PCa incidence in patients treated daily with 20 mg of toremifene for 6-12 months as compared with placebo.235 These promising results have prompted the initiation of a large phase III study to examine the potential for toremifene as a chemopreventive agent.

Phytoestrogens are another important class of estrogen-based chemopreventive agents. The most common phytoestrogens, soy isoflavones such as genistein, equol, and daidzein, which are abundant in soy beans and its products, have been found to have estrogenic or antiestrogenic activity.236-239 They have been some of the most popular agents in studies of the potential of PCa chemoprevention and therapy because of the low incidence of PCa, along with the high levels of phytochemicals, in the diets of Asian populations.240 Although there is an abundance of in vitro and in vivo experimental data about the anti-tumor properties of phytoestrogens, the value of their protective or therapeutic effects in cohort studies still remains controversial.241-248 Nevertheless, a number of phase I clinical trials evaluating the use of phytoestrogen supplementation in patients with PCa have generally seen beneficial effects without any toxicity.212;249-252

Summary

Traditionally, androgens have been considered to be the major sex hormones regulating the normal and malignant growth of the prostate. However, recent epidemiologic findings and experimental data suggest that estrogens and their mimics can be responsible for the pathogenesis of PCa. The carcinogenicity of estrogens in the prostate during adulthood is believed to be mediated by the combined effects of the hormone-induced unscheduled cell proliferation and epigenetic silencing of anti-tumor genes, along with the bioactivation of estrogens to genotoxic carcinogens. Thus, individuals or ethnic groups with polymorphisms in genes encoding ERs and/or estrogen-metabolizing enzymes can modify the risk for PCa caused by altered responsiveness to the hormone and exposure to its carcinogenic metabolites over a lifetime. The age-dependent hormonal shift from androgen to estrogen could also be an important contributing factor to increased estrogen bioavailability. Although PCa has a long latency and starts to develop in men around middle age, recent data strongly suggest that PCa risk could be determined even as early as during pre- and perinatal life stages by a process known as the “estrogen imprinting.” Thus, primary PCa prevention should probably begin in early life. Among the various cellular mediators, ERβ appears to be a key determinant in the pathogenesis, progression, and metastasis of PCa. Therapeutic approaches targeting its activation/inactivation may have important ramifications in the prevention and treatment of PCa. Epigenetic mechanisms such as DNA methylation play important roles in regulating the expression of the two ER subtypes. A change in the methylation status of proximal promoters of these genes constitutes an “on/off” switch for reversible gene regulation. Moreover, the differential expression of different ER-spliced variants (isoforms) could explain some conflicting observations related to estrogen action in the initiation and progression of PCa. Apart from the canonical genomic action of ERα and ERβ, the therapeutic potential of ER and its variants that function in multiple non-genomic pathways, such as membrane ERα, mitochondrial ER, ERRs, and GPR30, may further contribute to the pathogenesis of PCa. Finally, various estrogenic/antiestrogenic/SERM-like compounds have demonstrable efficacies in causing PCa regression through the utilization of pathways independent of the hypothalamic-pituitary axis. As the treatment of advanced PCa with transdermal estrogen has gained in popularity, several SERMS, including toremifene, have shown promise as chemopreventive or therapeutic agents in clinical trials. Although data from clinical trials are not conclusive, phytoestrogen supplements, including dietary soy, continue to be used by patients as complementary alternative medicine for PCa. With a greater understanding of the molecular mechanism underlying estrogen carcinogenicity in the prostate, the applicability of estrogen/antiestrogen-based prevention and treatment therapies, as first-line or adjuvant therapies, will gain ground in the clinic. Thus, it is timely in devising a new generation of estrogenic/antiestrogenic therapies with higher specificity against PCa and fewer off-target effects.

Acknowledgements

We thank Nancy K. Voynow for her professional editorial assistance and Dr. Xiang Zhang for helpful critiques of the manuscript. Research supported in part by NIH grants CA112532; ES006096; ES015584; ES018758; and ES018789.

Funding support: NIH grants: DK061084, CA112532, CA015776, ES006096, and ES015584 to Shuk-mei Ho; Department of Defense Prostate Cancer Program grant: PC030595 to Shuk-mei Ho and Department of Defense Breast Cancer Program grant: BC094017 to Ming-tsung Lee

Footnotes

Declaration of interest The authors have nothing to disclose.

Contributor Information

Shuk-Mei Ho, Department of Environmental Health, Center for Environmental Genetics, and the Cancer Institute, College of Medicine, University of Cincinnati, Cincinnati, Ohio.

Ming-tsung Lee, Department of Environmental Health, College of Medicine, University of Cincinnati, Cincinnati, Ohio. Telephone 513-558-0595, Fax 513-558-0071, lee2mg/at/mail.uc.edu.

Hung-Ming Lam, Department of Environmental Health, College of Medicine, University of Cincinnati, Cincinnati, Ohio. Telephone 513-558-0595, Fax 513-558-0071, lamhg/at/uc.edu.

Yuet-Kin Leung, Department of Environmental Health, Center for Environmental Genetics, and The Cancer Institute, College of Medicine, University of Cincinnati, Cincinnati, Ohio. Telephone 513-558-5181, Fax 513-558-0071, ricky.leung/at/uc.edu.

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