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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Invest Dermatol. Author manuscript; available in PMC 2012 April 1.
Published in final edited form as:
PMCID: PMC3164298
NIHMSID: NIHMS319054

IgE, IgM and IgG4 anti-desmoglein 1 autoantibody profile in endemic pemphigus foliaceus (Fogo Selvagem)

Ye Qian,1 Phillip Prisayanh,1 Eugenio Andraca,2 Bahjat F. Qaqish,2 Valeria Aoki,3 Gunter Hans-Filhio,4 Evandro A. Rivitti,3 and Luis A. Diaz1, for the Cooperative Group on Fogo Selvagem Research

An endemic form of PF (pemphigus foliaceus) exists and is virtually exclusive to certain inland states of Brazil, where it is known as Fogo Selvagem (FS) (Diaz et al., 1989b). FS shows similar clinical, histological and immunological features to those observed in non-endemic PF (Diaz et al., 1989a; Stanley et al., 1986). The etiology of FS is unknown, but blister formation is mediated predominantly by IgG4 (Rock et al., 1989). Moreover, a progression from preclinical to clinical disease is associated with a sharp rise in IgG4 anti-Dsg1 (Warren et al., 2003), and these autoantibodies are predictors of FS (Qaqish et al., 2009). Emergence of self-reactive IgG4 anti-Dsg1 in FS is a mystery; however, there are some promising circumstantial clues that point toward the etiology of the disease. For example, the antigen-selected nature of anti-Dsg1 (Qian et al., 2009) and the presence of IgM anti-Dsg1 in healthy individuals in endemic areas of FS (Diaz et al., 2008) strongly suggest a recent or an ongoing exposure of settlers in these endemic regions to an environmental antigen(s) (Aoki et al., 2004; Diaz et al., 2004; Diaz et al., 2008)

IgG4 and IgE antibodies develop in individuals chronically exposed to environmental allergens or during immunotherapy of patients with allergic diseases (Lichtenstein et al., 1968; Muller, 2005). Little is known about the IgE anti-Dsg1 response in FS, but recently Nagel et al (Nagel et al., 2009) have reported that IgE anti-Dsg3 correlated with disease activity in Pemphigus Vulgaris patients. In this investigation, we have evaluated the IgE, IgM and IgG4 anti-Dsg1 responses in a large cohort of FS patients and control individuals. A total of 558 sera were tested for IgE anti-Dsg1. Patient cohorts included 143 FS patients (Brazil), 39 non-endemic PF patients (USA and Japan), and 59 PV patients (USA and Japan). Healthy control (HC) cohorts included 161 healthy individuals living in endemic areas of FS (Brazil), 57 healthy individuals from non-endemic areas (Brazil), and 99 healthy individuals from USA (n= 76) and Japan (n= 23). Dsg1-specific ELISA were carried out as described (Qian et al., 2007) with a minor modification, i.e. the use of biotin labelled mouse anti-human IgE and HRP conjugated streptavidin (SouthernBiotech, Birmingham, AL). The levels of IgE anti-Dsg1 were expressed as index value units as reported by Amagai et al (Amagai et al., 1999) and Diaz et al (Diaz et al., 2008; Qaqish et al., 2009).

The distribution of IgE index values in sera from FS, PF, and healthy individuals from Brazilian, Japanese and US donors is shown as box plots (Figure 1a). The statistical comparisons of these groups are shown in Table 1. The IgE anti-Dsg1 index values in the FS group were higher than in the other sets, while HC from US and Japan showed the lowest values. The differences in means between the FS group and all other groups tested were statistically significant at the 0.05 level. The difference between HC from endemic regions of Brazil and non-endemic regions of Brazil was not significant. However, the difference between HC from Brazil and USA/Japan was statistically significant. The significantly different IgE anti-Dsg1 levels between the FS group and the PF USA/Japan indicate that the IgE anti-Dsg1 response in FS is a distinctive serological feature of this disease. The significant difference in IgE anti-Dsg1 between HC Brazil (endemic and non-endemic) and HC USA/Japan needs to be explored in the future. The lack of difference between HC endemic Brazil and HC non-endemic is likely explained by the fact that these groups share certain living conditions, and perhaps environmental antigens. These antigens however may not be present in USA and Japan.

Figure 1
IgE and IgG4 anti-Dsg1 in Fogo Selvagem patients and Control groups
Table 1
1 Comparison of the IgE anti-Dsg1 Autoantibodies in FS and Control Groups

We then analyzed the correlation between the IgE and IgG4 anti-Dsg1 responses in 143 FS and 317 HC. Figure 1b shows a plot of IgE against IgG4 anti-Dsg1, with superimposed regression lines, in the FS (red) and HC groups (blue). We found a correlation between IgE and IgG4 in the FS group (Spearman correlation r= 0.32, p<0.001), and less correlation in the HC group (r= 0.15, p= 0.07) suggesting that generation of these autoantibodies in FS may be linked and arising due to sensitizing to a common environmental allergen. These patterns of IgG4 and IgE responses are similar to those observed in allergic patients. Ongoing studies may demonstrate a stronger correlation between IgE and IgG4 anti-Dsg1 systems when testing sera from patients at different clinical and immunological stages of evolution.

To evaluate the humoral IgM, IgG4 and IgE anti-Dsg1 responses in FS and HC, we extracted data on IgM and IgG4 anti-Dsg1 for the same subjects utilized in previous publications (Diaz et al., 2008; Qaqish et al., 2009). An ROC curve for IgM, IgE and IgG4 anti-Dsg1 index values was used to determine a cutoff point for sensitivity and specificity as described (Diaz et al., 2008; Qaqish et al., 2009). The percentages of positive sera for IgM, IgE and IgG4 anti-Dsg1 in the different groups were plotted and shown in Figure 1c. It is apparent that the triple response of IgM, IgG4 and IgE anti-Dsg1 is much higher in FS patients than in HC from the same endemic regions and HC from non-endemic regions of Brazil and USA/Japan (χ2, p<0.05, for IgM, IgE, and IgG4 anti-Dsg1). The percentages of positive individuals for IgM and IgE anti-Dsg1 were higher in the FS group (58% and 81% respectively) than in all other groups (χ2, p<0.05). As expected and also shown in Figure 1c, IgG4 anti-Dsg1 was detected in a large number of FS, PF and PV (90%, 77% and 33% respectively), which is a known feature of these diseases. These findings suggest a persistent antigenic stimulation of the immune system of individuals living in the endemic areas of FS.

In summary, our findings further support the notion that the anti-Dsg1 response in FS patients may be initiated by sensitization to an environmental allergen(s). The cross-reactive IgE, IgM and pathogenic IgG4 anti-Dsg1 response may be the serological markers of these immunological responses.

Acknowledgments

Funding support is provided by NIH grants R01-AR30281, RO1-AR32599, T32 AR07369 (LAD), and K01-AR056378 (YQ). Dr. Qian’s research is also supported by a Dermatology Foundation Research Award and an American Skin Association Alice P. Melly Research Grant.

Abbreviations

Dsg1
desmoglein 1
Dsg3
desmoglein 3
FS
Fogo selvagem
PF
pemphigus foliaceus
PV
pemphigus vulgaris
rDsg1
recombinant Dsg1
ROC
receiver operating characteristic

Footnotes

CONFLICT OF INTEREST

The authors state no conflict of interest.

References

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