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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Nature. Author manuscript; available in PMC 2011 December 9.
Published in final edited form as:
PMCID: PMC3159410
NIHMSID: NIHMS313073

A genetically humanized mouse model for hepatitis C virus infection

Abstract

Hepatitis C virus (HCV) remains a major medical problem. Antiviral treatment is only partially effective and a vaccine does not exist. Development of more effective therapies has been hampered by the lack of a suitable small animal model. While xenotransplantation of immunodeficient mice with human hepatocytes has shown promise, these models are subject to important challenges. Building on the previous observation that CD81 and occludin (OCLN) comprise the minimal human factors required to render mouse cells permissive to HCV entry in vitro, we attempted murine humanization via a genetic approach. Here we show that expression of two human genes is sufficient to allow HCV infection of fully immunocompetent inbred mice. We establish a precedent for applying mouse genetics to dissect viral entry and validate the role of SCARB1 for HCV uptake. We demonstrate that HCV can be blocked by passive immunization, as well as show that a recombinant vaccinia virus (rVV) vector induces humoral immunity and confers partial protection against heterologous challenge. This system recapitulates a portion of the HCV life cycle in an immunocompetent rodent for the first time, opening opportunities for studying viral pathogenesis and immunity and comprising an effective platform for testing HCV entry inhibitors in vivo.

Humans and chimpanzees are the only species permissive to HCV infection. The basis for this highly restricted tropism is not completely understood, but may result from viral dependence on host factors present in only a few cell types. Murine cells are resistant to HCV entry, show inefficient viral replication, and may be blocked at later life cycle steps. HCV enters hepatocytes through the combined action of at least four host molecules: CD811, scavenger receptor type B class I (SCARB1)2, claudin 1 (CLDN1)3 and occludin (OCLN)4. We have previously shown that CD81 and OCLN comprise the minimal human factors required for HCV uptake by rodent cells4. This led to the hypothesis that expression of these human orthologs could render mice susceptible to HCV infection in vivo. We therefore constructed recombinant adenoviruses encoding human CD81, SCARB1, CLDN1 and/or OCLN. Intravenous delivery of these vectors resulted in 100 to 1000-fold overexpression of the corresponding mRNA in the murine liver and strong expression of all four proteins with the expected subcellular distribution (Supplementary Fig. 1). We determined that 18–25% of murine hepatocytes expressed human CD81 and OCLN together, while approximately 5% of cells expressed all four heterologous genes (Supplementary Fig. 2b–d). These results encouraged us to investigate infection of these animals. Unfortunately, HCV replication in mouse cells is inefficient in vitro and in vivo5,6,7,8,9. Consistent with this, challenge of mice expressing all four human factors with a firefly luciferase (Fluc)-encoding HCV genome [Jc1FLAG2(p7Fluc2A)] did not yield bioluminescent signal above background (Supplementary Fig. 3a). Direct measurement of Jc1FLAG2(p7Fluc2A) genome levels by quantitative reverse transcription (qRT)-PCR demonstrated a slight increase in HCV RNA in the serum (at 3h) and liver (at 3 and 24h); at 72h, however, the signal was reduced to background (Supplementary Fig. 3b–d). These data highlight the difficulty of detecting HCV infection in cell types that do not support robust replication. In mouse cells, this defect may result from incompatibility between the viral replication machinery and murine factors and/or from exacerbated murine innate antiviral responses. Furthermore, adenoviral gene delivery strongly induces interferon-stimulated genes, including viperin, IFI44, Mx1, 2’OAS, IP-10 and PKR, creating an environment that mimics recombinant IFNα treatment (Supplementary Fig. 4) and may antagonize HCV replication10.

As an alternate approach, we constructed a bicistronic HCV genome expressing CRE recombinase (Bi-nlsCre-Jc1FLAG2, abbreviated HCV-CRE), which activates a loxP-flanked luciferase reporter in the genome of the Gt(ROSA)26Sortm1(Luc)Kaelin (Rosa26-Fluc) mouse11. Hydrodynamic delivery of HCV-CRE RNA into Rosa26-Fluc mice led to reporter signal in the liver, indicating that CRE recombinase is active in the context of the HCV genome (Supplementary Fig. 5). Delivery of a polymerase-defective HCV-CRE RNA produced similar results, suggesting significant CRE production was derived from initial translation without the need for replication (Supplementary Fig. 5). To test whether mice could be infected by authentic HCV particles, we generated Rosa26-Fluc animals expressing human CD81 and OCLN, or all four human entry factors, and inoculated these mice with cell culture-derived HCV-CRE. In mice expressing all four transgenes, luciferase signals increased longitudinally, peaked at approximately 72 h post-infection, and decreased thereafter; mice lacking the transgenes did not show significant reporter activity (Fig. 1b and Supplementary Fig. 6). All animals expressing at least human CD81 and OCLN could be successfully infected. Loss of signal after 72h was likely due to strong anti-vector immunity, as evidenced by the increased frequencies of natural killer (NK) cells (Supplementary Fig 7a); depletion of NK cells prior to adenovirus injection prolonged luminescence activity (Supplementary Fig. 7c). Bioluminescent signals were dependent on the doses of both adenovirus and HCV-CRE (Supplementary Fig. 6), and infection was observed across a panel of chimeras expressing the structural proteins of diverse HCV genotypes (Fig. 3a). HCV core, NS3 or NS5A could not be detected (data not shown) probably owing to inefficient viral replication in murine hepatocytes. To estimate the number of HCV-infected liver cells, we therefore used an indicator mouse strain in which CRE leads to activation of a nuclear-localized green fluorescent protein/β-galactosidase (GNZ) reporter (B6.129-Gt(ROSA)26Sortm1Joe/J, abbreviated Rosa26-GNZ)12. We observed HCV-CRE infection in approximately 4–5% of murine hepatocytes expressing human CD81 and OCLN (Fig. 1c and Supplementary Fig. 2h) and up to 20% expressing all four factors (Fig 1c). We did not observe HCV uptake in non-parenchymal liver cells. These frequencies are consistent with previous estimates from primary human hepatocyte cultures13,14 and HCV-positive patient tissues15, although our system represents a single cycle infection and does not fully reflect the natural equilibrium. Taken together, these data provide, for the first time, evidence that mice can be rendered susceptible to HCV infection by a defined set of human genes.

Figure 1
Genetic requirements for HCV entry in vivo
Figure 3
Use of genetically humanized mouse model to evaluate vaccines against multiple HCV genotypes

Previous studies in Chinese hamster ovary (CHO) and mouse fibroblast (NIH3T3) cells have shown that CD81 and OCLN are the primary determinants of HCV tropism at the level of entry4. To investigate the in vivo determinants of species restriction, we delivered combinations of human or mouse CD81, SCARB1, CLDN1 and OCLN to Rosa26-Fluc mice. In accordance with the in vitro data, HCV permissivity increased 6–10 fold in mice expressing human CD81 and OCLN, compared to those expressing only murine factors. Notably, addition of exogenous human or mouse SCARB1/CLDN1 (Fig. 1d) boosted permissivity compared to human CD81/OCLN alone (Fig. 1b), suggesting that low endogenous entry factor levels may limit infection. While overexpression of mouse CD81 or OCLN in combination with the other human entry factors permits low-level uptake, human CD81 and OCLN are required for optimal infection. To verify authentic co-receptor function, we delivered entry factors bearing mutations previously shown to disrupt their activities. CD81 mutations F186L and E188K are known to abrogate soluble E2 binding and to reduce HCV uptake by human hepatocytes16. Indeed, expression of mutant human CD81 reduced in vivo entry efficiency by about 50% (Fig. 1e). A human OCLN chimera in which the second extracellular loop is replaced with the murine sequence has been shown to impair HCV entry in vitro by approximately four-fold4. Concordantly, HCV susceptibility of mice expressing the chimeric molecule was reduced by approximately 50% (Fig. 1e). These data indicate that infection of murine hepatocytes in vivo is dependent on the native functions of human CD81 and OCLN.

Unlike the human-restricted entry factors, mouse SCARB1 appears to support HCV uptake in vitro4 and in vivo (Fig. 1b). To validate the importance of this endogenous factor, we crossed mice with a disruption in Scarb1 (SCARB1−/−)17 to the Rosa26-Fluc background. Expression of human CD81, CLDN1 and OCLN in mSCARB1−/− Rosa26-Fluc mice reduced HCV-CRE infection efficiency by ~90%, as compared to SCARB1+/+, and ~50% compared to SCARB1+/− mice (Fig. 1f). This reduction appears to be a direct consequence of murine SCARB1 deficiency, and not transduction efficiency, as expression levels of CD81, CLDN1 and OCLN were similar in wild-type (Supplementary Fig. 1g) and SCARB1 knockout mice (Supplementary Fig. 8). Complementing mSCARB1−/− Rosa26-Fluc mice with human CD81, CLDN1 and OCLN and SCARB1 of either human or mouse origin fully restored infectivity (Fig. 1f). These data provide the first direct evidence that SCARB1 is a bona fide HCV entry factor in vivo. Taken together, these results suggest that infection of humanized mice occurs via authentic entry pathways, and highlight the value of this system for studying HCV co-receptor biology in vivo.

Despite considerable efforts, the lack of preventative or therapeutic HCV vaccines remains a major clinical challenge. Delivery of antibodies via passive immunization might be particularly suitable to block HCV entry, possibly limiting graft re-infection during liver transplantation and potentially boosting treatment success. Antibodies against E2 and CD81 have been shown to block HCV infection in cell culture13,18 and human liver chimeric mice19,20. We investigated passive immunization in the genetically humanized model. Delivery of anti-CD81 antibodies resulted in a dose-dependent inhibition of HCV-CRE infection, whereas isotype control immunoglobulins had no effect (Fig. 2a,d). Similarly, pre-incubation of HCV-CRE with antibodies directed against E2 (clone 3/11), but not an isotype control, significantly inhibited infection (Fig. 2b,e). These data further affirm that HCV is taken up in a glycoprotein-specific fashion in vivo and suggest the utility of this model for evaluation of passive immunization strategies.

Figure 2
HCV entry into murine hepatocytes in vivo can be blocked by antibodies or passive transfer of vaccine induced antiserum

Induction of broad-spectrum adaptive immunity is a key goal of HCV vaccine research. A promising candidate is a recombinant vaccinia virus (rVV) vector expressing HCV proteins, which has been shown to induce adaptive responses in rodents and chimpanzees21. To evaluate this candidate in genetically humanized animals, Rosa26-Fluc mice were immunized intraperitoneally with 1×107 plaque forming units (PFU) of rVV expressing the HCV proteins C-E1-E2-p7-NS2 (strain HCV-1, genotype 1a)22. Robust titers of anti-E2 antibodies (Fig 3b) and decreased susceptibility to heterologous challenge with HCV-CRE expressing the structural proteins of genotypes 1b, 2a, or 4a (Fig. 3c) were observed. Protection directly correlated with the levels of anti-E2 antibodies in the serum. Challenge with genotype 1b (strain Con1) HCV-CRE virus, whose envelope proteins are closely related to those of HCV-1, resulted in complete protection in two out of 10 animals (Fig. 3d, e, f). Pooled sera of immunized, but not non-immune, mice neutralized diverse HCV genotypes in vitro (Supplementary Fig. 9) and reduced infection by 50% when transferred passively (Fig. 2c,f). These data demonstrate the value of the genetically humanized mouse as the first immunocompetent small animal model amenable to HCV-challenge.

This study represents an important step forward in developing a robust small animal model for HCV infection and immunity. To our knowledge, this is the first time that any step in the viral life cycle has been recapitulated in a rodent simply by the expression of human genes. Previously developed liver chimeric mice are susceptible to HCV infection and support the entire viral life cycle2325. Despite recent improvements23, however, these models are hampered by low throughput, intraexperimental variability, and high costs26. Furthermore, the lack of a functional immune system negates their value for vaccination studies. The inbred mouse model presented here is not only susceptible to infection by diverse HCV genotypes, but it is also fully immunocompetent, providing the first small animal platform suitable for combined immunization and challenge studies. While it may eventually be desirable to transgenically express the HCV entry factors, adenoviral expression allows rapid testing of various mutant proteins on diverse backgrounds. The genetically humanized mouse model provides proof-of-principle for such applications and holds promise for assessing the immunogenicity and efficacy of HCV vaccines.

Methods summary

For more detailed methods see Supplementary Methods.

Mice

Gt(ROSA)26Sortm1(Luc)Kaelin11, B6;129-Gt(ROSA)26Sortm1Joe/J27 and B6;129S2-Scarb1tm1Kri/J17 and FVB/NJ wild-type mice were obtained from The Jackson Laboratory (Bar Harbor, ME). Mice were bred and maintained at the Comparative Bioscience Center of The Rockefeller University according to guidelines established by the Institutional Animal Committee.

Recombinant adenoviruses

Adenoviral constructs encoding human and murine homologues of the four HCV entry factors (CD81, SCARB1, CLDN1 and OCLN) and mutants thereof were created using the AdEasy™ Adenoviral Vector System (Agilent Technologies, Santa Clara, CA) according to the manufacturer’s instructions.

Hepatitis C virus

Plasmids encoding chimeric HCV genomes, including Jc1FLAG2(p7Fluc2A) and bicistronic HCV genomes expressing CRE, were linearized with XbaI and transcribed using MEGAscript T7 (Ambion, Austin, Texas). RNA was electroporated into Huh-7.5 cells using a ECM 830 electroporator (BTX Genetronics) and infectious virus was collected from supernatants 48–72 h after transfection.

Supplementary Material

Acknowledgements

We thank J. Sable, E. Castillo, A. Forrest, M. Panis, S. Pouzol, S. Shirley, A. Webson and Erick Giang for laboratory support, L. Chiriboga and H. Yee for excellent technical assistance, Jens Bukh and Apath, LLC for providing the prototype intergenotype HCV chimeras and C. Murray for editing the manuscript. This study was supported in part by award number RC1DK087193 (to C.M.R. and A.P.) from the National Institute of Diabetes and Digestive and Kidney Diseases, R01AI072613 (to C.M.R.), R01AI079031 (to M.L.) and R01AI071084 (to D.R.B.) from the National Institute for Allergy and Infectious Disease, The Starr Foundation and the Greenberg Medical Institute. M.D. was supported by postdoctoral fellowship from the German Research Foundation (Deutsche Forschungsgesellschaft) and M.T.C. by funds from The Rockefeller University’s Women & Science Fellowship Program. J.W.S. and C.T.J are recipients of Ruth L. Kirschstein National Research Service Awards from the National Institute of Health (F32DK082155 to J.W.S., F32DK081193 to C.T.J.).

Footnotes

Author contributions M.D., C.M.R. and A.P. designed the project, analyzed results and wrote the manuscript. M.D., J.A.H., J.B.R., W.B., Q.F., K.M, M.T.C. and M.L. performed the experimental work, J.W.S., C.T.J, and D.R.B. provided reagents.

Competing financial interests. The authors declare the following conflicts of interest, which are managed under University policy: C.M.R. has equity in Apath, LLC, which holds commercial licenses for the Huh-7.5 cell line and the HCV cell culture system.

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