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Striated muscle cells display an extremely regular assembly of their actin cytoskeleton that contributes to the contractile elements, the myofibrils. How this assembly is initiated and how these structures are maintained is still unclear. We have recently shown that striated muscle expresses a specific isoform of the formin protein family member FHOD3, which is characterised by the presence of a CK2 phosphorylation site at the C-terminal end of the formin homology domain 2 (FH2). Phosphorylated muscle FHOD3 displays a different subcellular localisation, namely to the myofibrils, and also has increased stability compared to un-phosphorylated or non muscle FHOD3. In addition, we could show that muscle FHOD3 is involved in myofibril maintenance in cultured cardiomyocytes and that its presence dramatically enhances the reconstitution of cardiac actin filaments after depolymerisation. Since FHOD3 expression levels and in particular that of the muscle isoform are also decreased in different types of cardiomyopathy, we postulate a crucial role for this protein in the maintenance of a fully functional cardiac cytoarchitecture.
Striated (cardiac and skeletal) muscle cells are characterised by their extremely regular cytoarchitecture. Actin and myosin are assembled into contractile elements that are arranged into para-crystalline myofibrils by a multitude of associated proteins (Fig. 1A).1,2 Currently it is still unclear, how exactly this precise assembly is regulated and also how protein turnover, either during normal maintenance or during adaptation to different working conditions e.g., by the swapping of isoforms, is achieved.3 Recent work has taken an inspiration from decades of research on the regulation of the actin cytoskeleton in migrating fibroblasts, where two major ways of controlling F-actin assembly were described: the ARP2/3 complex and its activator N-WASP leads to the formation of branched actin filaments, while different members of the formin protein family stimulate the assembly of unbranched actin filaments.4,5
It now begins to emerge that the assembly or maintenance of actin filaments in muscle cells may actually involve well-known players from cell migration.6–8 For example, in a paper that was recently published, we were able to show that a striated muscle specific splice variant of the formin FHOD3 plays a crucial role in the maintenance of actin filaments in cardiomyocytes in culture.6 The muscle isoform of FHOD3 is characterised by the inclusion of an additional exon that encodes for eight highly conserved amino acids at the C-terminal end of the formin homology 2 (FH2) domain and thereby introduces a CK2 phosphorylation site (Fig. 1B). This phosphorylation by CK2, which is a constitutively active and ubiquitously expressed kinase,9 has dramatic consequences on the subcellular localisation of muscle FHOD3, since it gets targeted to the myofibrils, and also on the protein stability, which is increased. Both aspects were also confirmed by using phosphomimicking constructs or constructs that could not be phosphorylated.6 An effect of phosphorylation by CK2 on protein stability and protein-protein interaction and thus subcellular localisation was previously already observed for e.g., the phosphatase PTEN or for beta-catenin,9,10 but this is to our knowledge the first time that a similar effect is seen for a cytoskeletal protein in muscle cells. Apart from its obvious consequences for protein stability and subcellular targeting, it is unclear at the moment, what kind of effect the phosphorylation would have on the function of FHOD3. Structural studies have established that two FH2 domains have to dimerise to a doughnut-like structure to enable actin polymerisation (for review including a very instructive movie see 11) and the CK2 site sits at the C-terminal end of the FH2 domain of FHOD3. CK2 phosphorylation has been suggested as an alpha-helix breaker12 and could have a similar effect in the case of muscle FHOD3. However, even if the alpha-helix is broken, judged by actin polymerization experiments that we carried out in HeLa cells, there is no distinguishable difference on the efficiency of muscle FHOD3 at least in that particular system.6 Due to our inability to express soluble recombinant FH1-FH2 constructs, we were not able to explore any potential differences in in vitro actin polymerisation assays. Therefore the detailed kinetic characterization of muscle versus non muscle FHOD3 in F-actin assembly as well as any effect on the 3D structure of the FH2 dimer doughnut remain to be investigated.
It is extremely intriguing that endogenous FHOD3 seems to be located in different parts of the sarcomere, depending, whether mature cardiomyocytes in situ or neonatal cardiomyocytes in culture are analyzed.6 In both mouse and human heart as well as in freshly isolated adult rat cardiomyocytes FHOD3 is found at the Z-disc, while in neonatal rat cardiomyocytes a broader localization as a doublet is observed, which is also seen for epitope-tagged full length FHOD3 (grey signal in Fig. 1A). A similar broader localisation is detected in embryonic mouse hearts (Iskratsch and Ehler, manuscript in preparation). We speculate that the Z-disc localization may be the default targeting in the “resting state” with only comparatively little thin filament turnover. The broader localization may reflect FHOD3 being busily involved in actin assembly. A more dynamic state of thin filaments in culture may explain the extremely rapid recovery of the actin cytoskeleton in neonatal rat cardiomyocytes (NRC) after disassembly, as apparent in our latrunculin B experiments. Already after 30 minutes reasonably restored actin filaments were seen, although as apparent from our RNAi knockdown experiments, only if FHOD3 was present in the system. Side to side comparison also revealed that at least under these conditions muscle FHOD3 is much more efficient than another formin, mDia-1, in the rescue.6 NRC have to rearrange their myofibrils in order to adapt from the 3D conditions in situ to the more or less 2D conditions in the culture dish and the presence of an extremely rapidly exchanging F-actin population in these cells was also reported by others.13
While the beneficial effect of FHOD3 on myofibril maintenance is obvious from our results and also from previous studies by Taniguchi and colleagues,14 it is still unclear whether FHOD3 or any other formin plays a crucial role during myofibrillogenesis in the embryo. We have observed FHOD3 expression from early on in the mesoderm of chick embryos and in mouse hearts at E9.5, when beating has just started (Iskratsch and Ehler, manuscript in preparation), but currently it is not known whether the presence of FHOD3 is essential for initial myofibril assembly. It may well be that several formins are involved, since recent data on Daam-1 (another member of the formin protein family) deficient mice showed impaired cardiac morphogenesis and also reduced myofibril assembly.15 However, from the preferential subcellular targeting of Daam-1 to the plasma membrane and its effect on the localization of alpha-catenin and N-cadherin in the adherens junctions of the embryonic cardiomyocytes, it appears more likely that Daam-1 plays a crucial role in the organization of the actin membrane cytoskeleton and the myofibril anchorage at these sites than directly in sarcomerogenesis.
While our work has focused on the role in myofibril maintenance of a formin that is highly expressed in the heart, others could show that N-WASP, which is another well known player in the regulation of the actin cytoskeleton via the Arp2/3 complex during cell migration, is also beneficial for myofibril growth, at least in skeletal muscle.8 N-WASP and formins share a similar mechanism of control of activity: in the inactive state an intramolecular interaction between the N-terminus and the C-terminus of the protein prevents access to the active sites in the middle of the molecule. This interaction can be relieved for example by the binding of small GTPases (Cdc42 for N-WASP, Rho/Rac-1 for formins).16 Interestingly, in skeletal muscle N-WASP gets activated by a different way, namely the interaction of its proline-rich domain with the SH3 domain of a striated muscle specific protein, nebulin and its function in this context appears to be independent from the Arp2/3 complex. Also N-WASP appears to be localized to different parts of the sarcomere in skeletal muscle cells, depending on the extent of myofibrillogenesis: at resting state it is found at the Z-disc, while following growth factor stimulation and therefore increased myofibril assembly its localization extends along the thin filament towards the M-band region.8 The Z-disc region of skeletal muscle is also, where gamma-cytoskeletal actin can be found in addition to muscle actin isoforms, apparently playing a role in long term muscle maintenance.17 Both the localization behavior of N-WASP and its intramolecular inhibition are reminiscent of FHOD3 and it seems to be likely that also other stalwarts of actin regulation during cell migration in fibroblasts may be involved in the process of myofibril assembly and maintenance. As with skinning cats, there would seem to be more than one way to maintain thin filaments in a muscle cell.
Work in the laboratory of Elisabeth Ehler was supported by a Medical Research Council Career Establishment Grant. Thomas Iskratsch was the recipient of a King's College London Strategic Investment Ph.D., Fellowship that was awarded to the Cardiovascular Division and a British Heart Foundation Research Excellence Centre Pump Priming Fellowship.