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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Neurosurg. Author manuscript; available in PMC 2011 August 8.
Published in final edited form as:
PMCID: PMC3151560
NIHMSID: NIHMS195076

Magnetic Source Imaging for the Surgical Evaluation of EEG Secondary Bilateral Synchrony in Intractable Epilepsy

Abstract

Objective

Routine scalp EEG cannot always distinguish whether generalized epileptiform discharges are the result of primary bilateral synchrony or secondary bilateral synchrony (SBS) from a focal origin, in which the latter may be amenable to surgical resection. Whole-head magnetoencephalography (MEG) has superior spatial resolution compared to traditional EEG and can potentially elucidate seizure foci in challenging epilepsy patients undergoing evaluation for surgery.

Methods

Sixteen medically intractable epilepsy patients with suspected SBS were referred for magnetic source imaging (MSI). All patients had bilateral synchronous, widespread, and most often generalized spike-wave discharges on scalp EEG, plus some other clinical (e.g. seizure semiology) or MRI feature (e.g. focal lesion) suggesting focal onset and hence possible surgical candidacy. MSI is the combination of whole-head MEG and parametric reconstruction of corresponding electrical brain sources. MEG and simultaneous EEG were recorded with a 275-channel whole-head system. Parameters of a single equivalent current dipole were estimated from the MEG data, and the dipole location and orientation was superimposed on patients’ MRIs.

Results

MSI revealed a focal dipole clusters in 12 of the total 16 patients (75%), of which a single dipole cluster was identified in seven patients (44%). Patient age, seizure type, seizure duration, VET and MRI results were analyzed to determine factors predictive of having focus clusters revealed on MSI. Of these factors, only focal MRI anatomic abnormalities were associated with focal MSI dipole clusters (Chi square test, P=0.03). Selective surgical resections (including the dipole cluster) in seven of eight patients (87%) resulted in seizure-free or rare seizure outcomes (Engels I and II).

Conclusions

These findings suggest an important role for MSI in the localization of focal onset in patients for whom there is a suspicion of SBS (based on clinical or MRI data). Identification of a focal seizure origin has significant implications for both resective and non-resective treatment of intractable epilepsy.

Keywords: magnetoencephalography, epilepsy, secondary bilateral synchrony, magnetic source imaging, epilepsy surgery

INTRODUCTION

Routine scalp EEG cannot always distinguish whether generalized epileptiform discharges are the result of primary bilateral synchrony (PBS) or secondary bilateral synchrony (SBS) from a focal origin. First described by Tukel and Jasper in 1952, SBS is defined as the rapid bilateral spread from a limited cortical area of electrical abnormality17. PBS, in contrast, is thought to have sources that are bilaterally symmetrical, with possible origination in subcortical structures projecting to multiple cortical areas, such as in genetic idiopathic generalized epilepsies 1,2. Misinterpretation of SBS as PBS has significant implications for patient management, as the former is often amenable to surgical remediation.

In SBS, cortico-cortical connections via the corpus callosum are thought to facilitate fast synchronization of epileptiform activity that arises from a focal source 1,2,5. It may be suggested by a consistent temporal relationship between a focal spike and an ensuing bilateral synchronous discharge, with a consistent time lag between the appearances of spike wave discharges in both hemispheres. In PBS, however, there is by definition no time lag between synchronization of activity in bilateral hemispheres 1,2,17. The region of maximum amplitude or of phase reversal may shift within an individual burst or indeed from burst to burst and unless a maximum is consistent, does not reliably differentiate PBS from SBS. Most reported cases of SBS arise from a focal source in the frontal lobe, followed by the temporal and parietal lobes 2. Frequently, medial frontal lobe sources such as a parasagittal meningioma can generate SBS, but cases have been also described in which the most likely sources were far from midline 17.

Methods of differentiating SBS from PBS include the application of independent component analysis of EEG5, estimation of interhemispheric small time difference during spike wave bursts10, and the use of high temporal resolution EEG tomographic source reconstruction12. Recently, whole-head magnetoencephalography (MEG) has been increasingly used to complement EEG in the evaluation of patients with epilepsy6,8,18.

MEG has several theoretical advantages over conventional low-density scalp EEG, including increased spatial resolution and sensitivity. MEG is not limited by conductivity issues, as is EEG, and is thought to be more sensitive to tangential sulcal sources. A few case reports have recently described the utility of MEG and its superimposition on structural MRI, referred to as magnetic source imaging or MSI, in the workup of patients with SBS15,16. Despite these advantages, the role of MEG in surgical evaluation of patients with partial epilepsies is unclear 11.

In this study, we aimed to describe our institution’s experience with MSI in the evaluation of selected patients with suspected SBS.

METHODS

We retrospectively reviewed the records of sixteen patients with medically intractable epilepsy whom underwent MSI between 2001 and 2006. We selected patients whose clinical referral indicated that they had generalized or diffuse ictal and/or interictal discharges on routine scalp EEG but were thought to have focal-onset seizures on the basis of seizure semiology or on the basis of cerebral lesion(s) seen on neuroimaging (i.e. the referring physician intended to use MSI to help distinguish primary from secondary bilateral synchrony). We did not have additional information that would allow us to ascertain patient diagnosis. All patients had a history of uncontrolled seizures despite multiple trials of antiepileptic drugs (AEDs). Protocols for retrospective analysis of patient data were approved by the Institutional Review Board at UCSF.

Patient demographics (age, gender) and pertinent epilepsy-related history (years since seizures began, seizure type, seizure frequency) were obtained from medical chart/records. For patients that underwent resective surgery, postoperative seizure control was also recorded using the Engel criteria: seizure-free (class I), rare seizures (class II), meaningful improvement (class III), and no improvement (class IV).

Interictal M/EEG recordings were done using simultaneous 275-channel whole cortex MEG and 21-channel scalp EEG. The clinical MEG data was band-pass filtered between 1 to 70 or 3 to 70 Hz and analyzed by an experienced MEG technologist (MM) and a board-certified clinical neurophysiologist (HEK). Spikes were chosen for analysis based on duration (<80 ms), morphology, and lack of associated artifact (e.g., ECG, EMG, EOG). Although the MEG readers had access to the simultaneous EEG to help distinguish spikes from artifact, they also selected spikes that met criteria but appeared only on MEG, since frequently individual spikes did not appear on both modalities. Spike onsets were chosen and corresponding dipoles were fit by using the commercial software supplied by the manufacturer (whole-head: CTF Systems, VSM, Port Coquitlam, BC); dipoles with >97% correlation and >95% goodness-of-fit were selected and superimposed on patients’ MRI scans (MSI) to produce magnetic source images (MSI). In several patients, automated interictal spike localization was done using an adaptive spatial filtering software application called Synthetic Aperture Magnetometry (SAM(g2))7.

RESULTS

Overall patient population

See Table 1 for patient demographics and seizure history. The median age was 19.4 years (range 3.5 to 45.1). The median duration from seizure onset was 12.4 years (range 1.8 to 35.1). Most patients had generalized tonic-clonic seizures (8/17) or complex partial seizures (7/17). One patient had simple partial seizures involving his left arm with secondary generalization.

Table one
Study cohort and seizure history

All patients underwent inpatient video EEG telemetry, some preceding their MEG referral and some following MEG referral (Table 2). Most interictal recordings showed simultaneous bihemispheric spikes or generalized spike-wave discharges. In eight patients, interictal spikes were bifrontal with unclear lateralization. In another seven patients, interictal spiking patterns were bilateral with a suggestion of hemispheric predominance but judged by the referring physician(s) to be non-lateralizing overall. Ictal onsets were recorded in 14 patients. These were typically generalized, demonstrating either simultaneous bilateral (mostly bifrontal) initiation or extremely rapid lead in from one side. One patient (case 5) showed interictal high amplitude sharp waves and slowing over the left hemisphere but an ictal pattern that had a slight lead from the right hemisphere.

Table 2
MRI, EEG, MSI, and Surgical Results for Patients with Secondary Bilateral Synchrony

Parametric single dipole source modeling revealed discrete focal dipole clusters in 12 of the total 16 patients (75%) on MSI (Table 2). In seven patients, there was only single dipole cluster. Patient age, seizure type, seizure duration, VET and MRI results were analyzed to determine factors predictive of having discrete localized clusters revealed on MSI (Table 3). Of these factors, only focal MRI anatomic abnormalities were associated with a focal MSI dipole cluster (Chi square test, P=0.03).

Table 3
Analysis of Predictors for Localizing MSI Dipole Clusters

Patients with focal anatomic abnormalities

Of the nine patients with focal MRI abnormalities, three had abnormal hippocampal morphology. Four patients had focal frontal cortical dysplasia. Two had focal post-traumatic encephalomalacia. MEG-derived equivalent current dipoles co-localized to the focal MRI abnormality in eight of nine patients (89%) (Cases 3, 5, 6, 7, 10, 11, 13, 14, and 15)(For examples, see Figure 1-Case 13 and Figure 2-Case 15).One patient exhibited abnormal left hippocampal morphology on MRI, but both EEG and MEG showed generalized spike wave discharges that could not be modeled using a single equivalent dipole model (Case 1).

Figure 1Figure 1
A 35 year-old man presented with long-standing generalized tonic clonic seizures (Table 2, Case 13). Routine EEG showed frequent bursts and runs of generalized spike and slow wave discharges on an otherwise normal background (A). His MRI revealed a subtle ...
Figure 2Figure 2
A 28 year-old man with history of generalized tonic clonic seizures since age 2 (Case 15). His MRI demonstrated abnormal left hippocampal morphology. Routine EEGs showed spike and spike and slow wave discharges that appeared generalized, though occasionally ...

Seven patients underwent surgical resection including areas with co-localized abnormalities on MRI and MSI (Table 2). Engel class I outcome was achieved in five patients at last follow-up following surgery (71%). One patient had rare seizures (Engel II), and another had no improvement (Engel IV) at 2 years after surgery. One patient could not undergo resective surgery since the putative focus was in dominant hemisphere superior temporal gyrus and instead underwent vagal nerve stimulator placement (Case 5). At two year follow-up, this patient had no reduction in seizure frequency or severity.

Patients with no MRI-based anatomic abnormalities

Of seven patients with normal appearing MRIs, four had focal MSI dipole clusters and three had multi-focal scattered dipoles. One underwent a left temporal lobectomy including one of three clearly identified dipole clusters, and had Engel class II outcome at six- to 24- months (Figure 3 – Case 8). Another two had interictal activity in cortical areas that were deemed inoperable given localization to the dominant hemisphere superior temporal gyrus in one patient (Case 14), and to the right frontal insula in the other (Case 5- this patient underwent placement of a vagal nerve stimulator and did not have improvement). The other three patients had multi-focal scattered interictal spike dipoles and no localizing or lateralizing evidence to allow further presurgical workup (Figure 4-Case 4) (Cases 2 and 16).

Figure 3
A 24 year-old man with history of generalized tonic clonic seizures since age 5 (Table 2, Case 8). He had multiple normal MRIs. Routine EEGs were said to show generalized spike and slow wave discharges with a bifrontal emphasis. MEG with simultaneous ...
Figure 4Figure 4
A 15 year-old boy with an eight year history of complex partial seizures consisting of staring spells and unresponsiveness (Table 2, Case 4). MRI was normal and routine EEG was said to show generalized sharp and slow wave discharges with a frontal maximum. ...

DISCUSSION

In this study, we have described a cohort of patients with medically refractory epilepsy that were referred for MEG evaluation with suspicion of SBS. These patients had demonstrated clinical or MRI findings that were suggestive of focal or lateralized epileptiform sources, but their routine scalp EEGs had generalized or bilaterally synchronous discharges. We found that MEG was able to reveal a focal area of interictal spiking in a relatively high proportion (75%) of patients in this selected group with high pretest probability of focality. The presence of an anatomic abnormality on MRI was associated statistically with co-localized dipoles on MSI. MSI results supported the decision to pursue surgical resection in eight patients. Resections included the MSI dipole cluster in all cases, except for two cases. All of patients for whom a single cluster was identified had Engel class I or II outcomes at 12- and 24-months following surgery, confirming that they did have focal epilepsy whose source was identified on MSI.

In 1985, Blume and Pillay laid out strict EEG criteria for SBS in their study of 57 patients1. The criteria required that spikes leading to SBS must last for two seconds, and that the morphology of focal triggering spikes must be different from that of the bisynchronous paroxysm, and similar to other focal spikes from the same region. In this classic scenario, where a focal spike train is followed by a broad synchronous burst, EEG and MEG should, in theory, both have similar abilities to identify the source, since they share similar temporal resolutions. However, this strict definition of SBS is not universally applicable, since absence of these features does not exclude SBS: a cortical focus may lie deep within a sulcus and initial discharges from it may be invisible to routine scalp EEG; or secondary generalization from a focus may be very rapid 1,2,16. Thus, typical cases encountered in practice do not include a localized spike or spikes preceding the generalized burst. In these cases, whole-head MEG using 100-300 sensors offers superior spatial resolution in dipole localization when compared to routine low-density scalp EEG. As pointed out by other authors, the single dipole ECD model that is used in routine MEG analysis (including here), despite or perhaps because of its limiting assumptions, can in practice resolve the generalized burst to a single source9,11.

Demonstration of an anatomic abnormality seen on MRI was the only factor associated with having a focal MSI dipole cluster. The additional confirmation from MSI when the EEG is equivocal is an important factor in the decision-making process to undergo surgery, and in some cases may help to tailor the resection in combination with electrocorticography 4. Many other studies have highlighted the predictive value of MRI abnormality in the surgical outcome for epilepsy; however, it cannot be relied upon exclusively3.

In cases in which the MRI is normal, some recent reports have validated use of the MSI to guide surgery13. The success of this approach was dependent upon close concordance between EEG and MEG. In the relatively infrequent case of SBS, however, the low-density scalp EEG is often too ambiguous to guide surgical planning. However, more sophisticated EEG techniques, including higher-density recording with source localization, as well as simpler modeling algorithms such as sequential voltage mapping have also recently shown promise in source localization 19,20.

Overall, our data suggest that MEG is a useful tool to evaluate difficult cases of epilepsy in which the EEG suggests SBS. The yield of MSI appears to be highly dependent upon whether an anatomic abnormality is seen on MRI, but may also prove useful in some cases where MRI is normal. The sensitivity in this case is impossible to determine, however, as the presence of a lesion on MRI in the face of a generalized EEG pattern was an important determinant of patient referral for MSI.

There are several limitations to our study. Although other papers on MEG evaluation of SBS have been described for one or two patients, our cohort of 16 patients is still relatively limited. In addition, our ability to document and to ascertain the diagnoses of the patients included is limited as we only had the limited clinical information provided to us by the referring physician in many cases; this means that in our group, we can not rule out the possibility that some individuals had both primary generalized and partial epilepsy 14. As source modeling algorithms improve to include multiple simultaneous or rapid sequential dipoles, MEG may offer a fuller explanation of the phenomenon of SBS. Surgical resection confirmed the source in some patients, but there was no independent information to achieve confirmation for those patients that did not undergo surgery.

CONCLUSION

Interictal MEG appears to be a useful noninvasive technique in the evaluation of patients with suspected SBS based on history, routine scalp EEG, and MRI evaluation. Patients with MRI abnormalities appear to be more likely to have a focal MSI dipole cluster adjacent to or co-localized with the abnormality, many of which may be amenable to surgical excision. MSI can help confirm that the sites of anatomic abnormality correlate to sites which generate seizures. Successful identification of epileptogenic foci may lead to surgical resection as a meaningful therapeutic option.

References

1. Blume WT, Pillay N. Electrographic and clinical correlates of secondary bilateral synchrony. Epilepsia. 1985;26:636–641. [PubMed]
2. Daly D. Current practice of clinical electroencephalography. 2. Philadelphia, PA: Lippincott-Raven; 1997. Secondary bilateral synchrony; pp. 310–311.
3. Holmes MD, Wilensky AJ, Ojemann GA, Ojemann LM. Hippocampal or neocortical lesions on magnetic resonance imaging do not necessarily indicate site of ictal onsets in partial epilepsy. Ann Neurol. 1999;45:461–465. [PubMed]
4. Iida K, Otsubo H, Matsumoto Y, Ochi A, Oishi M, Holowka S, et al. Characterizing magnetic spike sources by using magnetoencephalography-guided neuronavigation in epilepsy surgery in pediatric patients. J Neurosurg. 2005;102:187–196. [PubMed]
5. Jung KY, Kim JM, Kim DW, Chung CS. Independent component analysis of generalized spike-and-wave discharges: primary versus secondary bilateral synchrony. Clin Neurophysiol. 2005;116:913–919. [PubMed]
6. Kirsch HE, Mantle M, Nagarajan SS. Concordance between routine interictal magnetoencephalography and simultaneous scalp electroencephalography in a sample of patients with epilepsy. J Clin Neurophysiol. 2007;24:215–231. [PubMed]
7. Kirsch HE, Robinson SE, Mantle M, Nagarajan S. Automated localization of magnetoencephalographic interictal spikes by adaptive spatial filtering. Clin Neurophysiol. 2006;117:2264–2271. [PubMed]
8. Knowlton RC, Laxer KD, Aminoff MJ, Roberts TP, Wong ST, Rowley HA. Magnetoencephalography in partial epilepsy: clinical yield and localization accuracy. Ann Neurol. 1997;42:622–631. [PubMed]
9. Knowlton RC, Shih J. Magnetoencephalography in epilepsy. Epilepsia. 2004;45(Suppl 4):61–71. [PubMed]
10. Kobayashi K, Ohtsuka Y, Oka E, Ohtahara S. Primary and secondary bilateral synchrony in epilepsy: differentiation by estimation of interhemispheric small time differences during short spike-wave activity. Electroencephalogr Clin Neurophysiol. 1992;83:93–103. [PubMed]
11. Lau M, Yam D, Burneo JG. A systematic review on MEG and its use in the presurgical evaluation of localization-related epilepsy. Epilepsy Res. 2008;79:97–104. [PubMed]
12. Morales-Chacon L, Bosch-Bayard J, Valdes-Sosa P, Ortega-Perez MA, Zaldivar M, Sanchez A. Brain electromagnetic tomography distinguishes primary generalised discharges from secondary bilateral synchrony. Rev Neurol. 2003;36:498–499. [PubMed]
13. RamachandranNair R, Otsubo H, Shroff MM, Ochi A, Weiss SK, Rutka JT, et al. MEG predicts outcome following surgery for intractable epilepsy in children with normal or nonfocal MRI findings. Epilepsia. 2007;48:149–157. [PubMed]
14. Sisodiya SM, Free SL, Stevens JM, Fish DR, Shorvon SD. Widespread cerebral structural changes in patients with cortical dysgenesis and epilepsy. Brain. 1995;118(Pt 4):1039–1050. [PubMed]
15. Smith MC. The utility of magnetoencephalography in the evaluation of secondary bilateral synchrony: a case report. Epilepsia. 2004;45(Suppl 4):57–60. [PubMed]
16. Tanaka N, Kamada K, Takeuchi F, Takeda Y. Magnetoencephalographic analysis of secondary bilateral synchrony. J Neuroimaging. 2005;15:89–91. [PubMed]
17. Tukel K, Jasper H. The electroencephalogram in parasagittal lesions. Electroencephalogr Clin Neurophysiol Suppl. 1952;4:481–494. [PubMed]
18. Wheless JW, Willmore LJ, Breier JI, Kataki M, Smith JR, King DW, et al. A comparison of magnetoencephalography, MRI, and V-EEG in patients evaluated for epilepsy surgery. Epilepsia. 1999;40:931–941. [PubMed]
19. Yoshida F, Morioka T, Hashiguchi K, Miyagi Y, Nagata S, Ohshio M, et al. Display of the epileptogenic zone on the frontal cortical surface using dynamic voltage topography of ictal electrocorticographic discharges. Minim Invasive Neurosurg. 2007;50:37–42. [PubMed]
20. Zumsteg D, Andrade DM, Wennberg RA. Source localization of small sharp spikes: low resolution electromagnetic tomography (LORETA) reveals two distinct cortical sources. Clin Neurophysiol. 2006;117:1380–1387. [PubMed]