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The Hsp70 chaperones (Heat shock protein 70 kDa) are key to cellular protein homeostatis. However, they also have the ability to inhibit tumor apoptosis, and contribute to aberrant accumulation of hyperphosphorylated tau in neuronal cells affected by tauopathies, including Alzheimer’s disease. Hence, Hsp70 are increasingly been identified as targets for therapeutic intervention in these widely abundant diseases. Hsp70 proteins are allosteric machines and offer besides classical active site targets, also opportunities to target the mechanism of allostery. In this work, it is demonstrated that the action of the potent anti-cancer compound MKT-077, is through differential interaction with the Hsp70 allosteric states. MKT-077 (1-ethyl-2-[[3-ethyl-5-(3-methylbenzothiazolin-2-yliden)]-4- oxothiazolidin-2-ylidenemethyl] pyridinium chloride) is therefore an “allosteric drug”. Using NMR spectroscopy, the compound’s binding site on human HSPA8 (Hsc70) is identified. The binding pose is obtained from NMR-restrained docking calculations, subsequently scored by molecular dynamics-based energy and solvation computations. Suggestions for improvement of the compound’s properties are made on the basis of the binding location and pose.
The Hsp70 chaperones (Heat shock protein 70 kDa, systematic name: HSPA) assist in protein folding, protein refolding, protein transport and protein targetting1. The human genome contains 13 HSPA genes which encode 12 distinct proteins2. HspA5 (Bip), HSPA8 (Hsc70) and HSPA9 (mt-Hsp70 or Mortalin) are expressed constitutively. They are resident in the ER, cytosol and mitochondria, respectively. Cells respond to stress such as, but not limited to, elevated temperature, by upregulating the expression of the other HSPAs, which help reverse the stress-induced protein misfolding. The chaperones carry this out by unfolding the misfolded proteins in ATP-driven cycles of binding and release3.
HSPAs are significantly up-regulated in tumors4, and are required for the survival of these cells5,6; 7. Knockdown of HSPA2 by siRNA inhibits human tumor growth in nude mice8. Selective HSPA1 knockdown induces apoptosis in pancreatic cancer cells9. Enhanced expression of HSPAs in tumor cells is likely caused by conditions which mimic stress10; 11. The HSPAs are thought to attempt to neutralize the conformational changes in mutated proteins12,13 which are common in tumorogenic cells. In addition, HSPAs are found to specifically inhibit cell death pathways11; 14; 15; 16; 17; 18. Lastly, HSPA9 may directly inactivate p53 tumor suppressor protein19; 20. Hence, inhibition of HSPA has been recognized as a promising avenue for the prevention or therapy of a wide variety of cancers.
HSPAs are also involved in several CNS disorders. Diseases such as Alzheimer’s, Pick’s disease, progressive supranuclear palsy, corticobasal degeneration and argyrophilic grain disease are characterized by the aberrant accumulation of hyperphosphorylated tau, called tau-tangles21; 22; 23; 24. HSPAs participate in the clearance of tau-tangles through a mechanism that is not well understood as of yet25. Recently, we found that inhibitors of HSPA8 led to a rapid increase in tau ubiquitination and proteasome-dependent degradation, in tau-overexpressing HeLa cells26. Clearance of tau-tangles is being recognized as therapeutic to Alzheimer-affected neuronal cells27. Hence, inhibition of HSPA is also a promising avenue for the prevention or therapy of CNS disorders28.
HSPA proteins are becoming recognized as very druggable29 because they offer so many opportunities for interference. HSPA proteins consist of a nucleotide-binding domain30 (NBD) which competitively binds ATP and ADP31, and substrate-binding domain (SBD) which harbors the hydrophobic substrate-binding cleft32. Compounds that compete for binding in either of these sites would modulate HSPA function. Examples of such compounds are adenosine analogs dibenzyl-8-aminoadenosine33; 34 and VER-15500833, which compete for the ATP binding site. Compounds that modulate HSPAs by competing with substrate binding such as Gentamycin35, geranylgeranylacetone36 and several peptidomimetics37 have been described as well.
In addition, HSPA are regulated by the DnaJ co-chaperone family and by a diverse set of nucleotide exchange factors38. They also interact with HIP39, HOP40 and CHIP41, and some specialized factors such as ZIM (for HSPA9)42. Competing with or enhancing the interaction of these ancillary proteins with HSPA may also be an avenue for HSPA modulation. One of the first HSPA inhibitors, 15-deoxyspergualin, was long known to bind to the C-terminal EEVD sequence43; 44. This C-terminus is now also known to be the CHIP-HSPA interaction site45, so likely 15-deoxyspergualin functions by competition with CHIP. Recently, we discovered an inhibitor of the bacterial HSPA orthologue DnaK, that most likely functions by competing with the J-HSPA protein interaction46 .
Finally, HSPA are allosteric proteins. Substrate binding enhances ATP hydrolysis while ATP binding stimulates substrate release. Recently, much insight in the allosteric mechanism has been gained from comparing the structural properties of HSPA in the ADP-state, bound to substrate (referred to as HSPA-ADP-SUB), and in the HSPA-ATP-APO state. HSPA-ADP-SUB is best described as a dynamic ensemble in which NBD and SBD are loosely tethered47; 48, while the NBD and SBD in HSPA-ATP-APO are docked47. In the HSPA-ADP-SUB state, a protective LID docks to the SBD41, while the LID releases in the HSPA-ATP-APO state. The subdomains of the NBD rearrange and reorient between ADP and ATP state49. A surface cleft on the NBD opens and closes between these states40,49. Compounds that can recognize such allosteric differences and perturb the allosteric equilibrium between the states should also modulate HSPA function.
Recently, we identified a flavonoid called myricetin, which binds to the Hsp70 of E. coli, DnaK, at an non-canonical site on he nucleotide binding domain. This compound blocks the binding of DnaK to DnaJ.50 It is the first example of an allosteric HSPA drug.
Taken together, there are many opportunities for the manipulation of HSPA function. In principle, the protein could be targeted by a combination of compounds that are individually not very potent, but act in synergy. In analogy to tumor irradiation therapy, using several low-intensity beams reinforcing each other at the target, a molecular target-specific combination therapy may also have reduced toxicity. In addition, the potential of addressing several different HSPA functions with small compounds may provide an avenue to HSPA-directed personalized medicine.
In the current work, we investigate the interaction of a well-known HSPA interacting compound, MKT-077. MKT-077 is known to interact with HSPA951 and HSPA852. We show here that MKT-077 affects HSPA8 function through differential interaction with HSPA’s different allosteric states. Hence, MKT-077 is found to be an “allosteric drug”. MKT-077 is a Fuji dye compound with the systematic name (1-ethyl-2-[[3-ethyl-5-(3-methylbenzothiazolin-2-yliden)]-4-oxothiazolidin-2-ylidenemethyl] pyridinium chloride) (see Figure 1). MKT-077 has an IC50 of 0.35-1.2 uM against several human cancer cell lines53. The IC50 of MKT-077 towards these tumor cell lines is more than 100 times lower than the IC50 against healthy cell lines. Because of these very favorable properties, MKT-077 has been evaluated as cancer chemo-therapeutic in a Phase I trial 54. The trial was aborted because of renal toxicity of MKT-077. Despite this finding, interest in MKT-077 and its derivatives have remained strong 55,56; 57.
As mentioned above, we recently found that inhibitors of HSPA8 also lead to a rapid increase in tau ubiquitination and proteasome-dependent degradation, in tau-overexpressing HeLa cells26. We show here that MKT-077 also enhances tau clearance, which makes the compound also of interest for therapy of CNS disorders such as Alzheimer’s. We determine by NMR the binding site of MKT-077 to the ADP-state of HSPA8 (Hsc70). The drug locates itself in a negatively charged pocket close to, but not identical to, the nucleotide binding site. The identification of its binding pocket and binding pose should allow for the design of more potent, more selective, and less toxic MKT-077 derivatives.
Inhibition of the HSPA8 by small molecules such as methylene blue or azure c causes clearance of tau-tangles in transfected HeLa cells26. It was hypothesized that the compounds interfere with the dissociation of HSPA8-tau complexes, leading to clearance through the ubiquitin-proteasome system26. Based on this, we wondered whether the known HSPA951 and HSPA852 inhibitor, MKT-077, would also lead to clearance of hyper-phosphorylated tau. Figure 2 shows that such indeed is the case, suggesting that MKT-077 also interacts and interferes with the function of HSPA8 in these cells. Inhibition of HSPA8 with MKT-077 therefore is a potential avenue for therapeutic intervention with tauopathic diseases such as Alzheimer’s.
In recent work, we have used NMR spectroscopy to locate the binding sites of several compounds to the bacterial Hsp70 chaperone, DnaK46; 50; 58. Here we use the same approach, combined with extensive computer modeling and molecular dynamics calculations, to decipher the binding location, pose and mechanism of MKT-077 with the nucleotide-binding domain (NBD) of human HSPA8.
The 15N-1H TROSY NMR spectrum of HSPA8 NBD in the ADP state is shown in the supplemental material. Many of the resonances in the spectrum of this 383-residue protein have been assigned by hand59 and double-checked by a computer algorithm60. Enlargements of the sections of the spectrum are shown in Figure 3. A select number of resonances show gradual chemical shift changes upon addition of MKT-077 up to a molar ratio of 1:1 (using a concentrated sample of 2H,15N,13C–labeled HSPA8-NBD) after which the chemical shifts do no change any more (using a more dilute sample of 15N–labeled HSPA8-NBD). The observed changes in chemical shifts for these two experiments are shown on the amino-acid sequence in the supplemental material.
Figures 4a and b show the composite chemical shifts observed depicted on a surface rendition of HSPA8 NBD. The majority of the shift changes take place in an area which turns out to be an otherwise unoccupied negatively charged pocket located on the interface of subdomains IA and IIA. As outlined in the introduction, HSPAs are allosteric proteins, which show global conformational and dynamical changes on the NBD between the ADP and ATP state59; 61. Hence, we wondered if MKT-077 would also bind to the ATP-state of the NBD. Figures 4c and d shows a similar display of changes in the TROSY spectrum of HSPA8 NBD in the AMP-PNP state. Significantly, none of the residues affected by MKT-077 binding in the ADP state are affected in AMPPNP state. We conclude that MKT-077 does not bind to Hsc70 NBD in the AMP-PNP state. We also monitored MKT-077 binding to HSPA8 NBD in the true ATP state. No chemical shift changes in the (largely unassigned) NMR spectrum could be discerned, indicating that MKT-077 does not bind to the ATP-state (not shown), indicating that the results for the AMP-PNP state are representative for the ATP state. We also monitored MKT-077 binding to HSPA8 NBD in the apo state. Many changes occurred in the NMR spectrum (not shown) but those were largely uninterpretable because a lack of NMR assignments for this state. Nevertheless, we may conclude that MKT-077 also binds to the apo-state.
The MKT-077-induced changes to the spectrum of HSPA8 NBD in the ADP state are in the fast-exchange limit (see Figure 3), with little or no line broadening. The largest shifts in the spectrum are approximately 20 Hz, which sets a lower limit of 100 s−1 for MKT-077’s off-rate. This sets the limit KD > 1 uM, when assuming an upper limit on the diffusion-controlled on-rate of 108 M−1s−1. The shifts are linear over the titration interval (see Figure 5), and saturate abruptly at equivalence (see Figure 3a and 3c). This is compatible with a KD that is at least order of magnitude smaller than the protein concentration; with Hsc70 concentration of 200 uM, we obtain KD < 10 uM. In combination, thus, we estimate from the NMR titration 1 uM < KD < 10 uM. Attempts to determine the binding affinity of MKT-077 to Hsc70 by ITC have not been successful.
HSPA8 NBD is a flexible molecule, which can rotate its subunits with respect to each other, especially in the ADP and apo states59; 61. Hence, chemical shift changes at the interface between sub-domains should be eyed with some skepticism. Indeed, there is an area of chemical shift changes (residues 140-144; see Fig 2 of supplemental material) which is not contiguous with the primary shift area shown in Figure 4a. The former area is completely buried, and the shifts must be caused by conformational changes due to MKT-077 binding at the primary and solvent-accessible pocket comprised by the residues shown in figure 5.
The entrance to pocket located on the interface of subdomains IA and IIA is hydrophobic, while the interior is negatively charged due to the presence of the sidechains of Glu175, Asp199 and Asp206. As matter of fact, the electrostatic potential of the entire binding area is strongly negative (see Figure 6). Hence, the chemistry of the binding area complements the chemical properties of MKT-077, which is positively charged, very well (see Fig 1). In support of the hypothesis that electrostatics is a important contributor to the binding energy of MKT-077 is the fact that an uncharged MKT-077 derivative does not perturb the NMR spectrum of HSPA8 (results not shown).
Together, the evidence strongly suggests that MKT-077 binds preferentially to the ADP- and not the ATP-bound state of HSPA8. To test whether this compound could stabilize the ADP-like conformation, we performed limited proteolysis experiments. It has been previously shown that trypsin treatment of human Hsc70 yields digestion patterns diagnostic of the chaperone’s nucleotide state62. Consistent with those reports, we confirmed that Hsc70 saturated with ATP was primarily cleaved into three high molecular weight bands, including prominent bands at approximately 69 kDa (band 1) and 66 kDa (band 2) (Figure 7). Conversely, treatment with ADP strongly favors band 2. Addition of the J-domain of prokaryotic DnaJ, which stimulates ATP turnover in Hsc70, converted the ATP-like pattern to an ADP-like pattern. Next, we tested whether MKT-077 could stabilize the ADP-like configuration. Addition of MKT-077 (200 μM) largely blocked formation of band 1, consistent with the NMR data that this compound binds preferentially to the ADP-bound state of Hsc70.
Our key finding is that MKT-077 binds to the ADP state of HSPA8, but not to the ATP state, which are the two most extreme allosteric states that an HSPA can adopt. The compound binds close to, but not at the natural nucleotide binding site. This qualifies MKT-077 as an allosteric effector, acting not unlike 2,3-DPG that modulates hemoglobin function by binding to a non-heme site that is exposed in the T-state and closed in the R-state63. MKT-077 binds to the interface of the four HSPA NBD subdomains. We have recently shown that the HSPA NBD subdomains change their relative orientations between the different allosteric states61 (see Fig 8). In detail, it was found (a) that the relative orientations of the subdomains of the HSPA ortholog DnaK of Thermus thermophilus in the ADP state resemble, in solution, those of HSPA8 complexed with nucleotide exchange factor as seen in a crystal, and (b) that the relative orientations of the subdomains of the HSPA ortholog DnaK of Thermus thermophilus in the AMP-PnP state resemble, in solution, those of HSPA8s NBD in a crystal, irrespective of the latter’s nucleotide state. We defined the ATP state as the “closed” state and the ADP state as the “open” state. In this context, the binding of MKT-077 to the ADP (open) state and not to the ATP (closed) state makes perfect sense: the binding pocket is (more) accessible in the ADP state but (less) not in the ATP state (see Figure 8).
These observations lead us to formulate the following hypothesis of the mechanism of action of MKT-077 (see Figure 9). The figure summarizes a large body of structural, dynamical and interaction characteristics for the HSPA8, based on the work of our48; 59; 61 and other labs, especially those of L. Gierasch47; 64 and M. Mayer/B.Bukau65; 66; 67. Briefly, in the ADP-substrate-bound state, the nucleotide binding cleft is mostly open and the substrate binding domain is loosely tethered. The lid is docked to the substrate domain. In the ATP-apo state, the nucleotide binding cleft is closed, while the substrate binding domain is docked. In this state, the lid is dynamically tethered.
In the current work, we observed that MKT-077 selectively binds to the ADP-allosteric state. By doing so, it likely stalls the HSPA protein-refolding cycle in that state. As a consequence, the HSPA cannot release its substrate. In the context of tauopathies, one hypothesizes68 that the lifetime of the tau-HSPA8 complex becomes long enough to engage in a triple complex with CHIP (C-terminal Hsp70 Interacting Protein). CHIP is an ubiquitin ligase69, which promotes the ubiquitination of the HSPA8-MKT trapped tau, and clearance by the proteasome.
Interference of MKT-077 with HSPA’s substrate binding and release cycle should also directly interfere with HSPA’s abilities to promote survival of tumor cells.
We used AUTODOCK70 to obtain insight in the MKT-077 binding geometry at this pocket. Based on the information that MKT-077 only binds to the ADP state, we used the crystal structure of HSPA8 NBD71 in complex with Hsp110 (3C7N) for the protein coordinates (see figure 8c). The selection of this particular HSPA-NEF complex (there are 5 different ones in the Protein Data Bank) is based on the fact that it is the only one that contains ADP. The crystal coordinates of the NBD were minimized, equilibrated and relaxed to 300 K using a Amber11 72 molecular dynamics protocol (see Methods). The coordinates and degrees of freedom for MKT-077 were optimized and determined from quantum mechanical calculations.
We used an AUTODOCK search box localized around the NMR-determined MKT-077 binding site. The results are shown in Figure 10a. On the one hand, there is satisfactory agreement between the AUTODOCK results and the NMR data: all good docks are located in the area identified by NMR chemical shifts. On the other hand, the AUTODOCK computations yield a wide variety of possible binding poses with AUTODOCK energies ranging between 4 and 7 kcal/mol. The electrostatic interaction does not exceed 0.4 kcal/mol in any of these calculations, which we feel is at odds with the prominent role of electrostatics in MKT-077 action. After culling those dockings which have positive MKT charges contacting positive protein residues, we subdivide the dockings in four families as shown in Figures 10 C-F.
All docked families are in close vicinity / contact with the residues that show chemical shift perturbations. All of them are also close to Glu 175 and Asp 206 that reside deep within the pocket and contribute much to the negative electrostatic potential of the pocket. These residues, as well as the other residues that are in contact with the MKT-077, are 100% conserved between the 13 human HSPA isoforms (see Table 1). Hence, it is more than likely that MKT-077 also binds at this site in the mitochondrial protein, HSPA9, which was earlier identified as prime target of the drug73; 74,51.
One needs a detailed description of the interaction of MKT-077 with HSPA, to suggest where modifications can be made to improve MKT-077. However, our AUTODOCK computations cannot distinguish between different orientations of the compound. For instance, in the AUTODOCK family depicted in Fig 10C, the positive pyridinium ring is reaching into the pocket to contact Glu 175 and Asp 206. In the family depicted in Fig 10D, partial contact occurs. In the other families, the positive pyridinium ring is solvent-exposed. In family 10F, it is in contact with Asp 225, which lines the rim of the pocket (at 2 o’clock in Figure 10F). AUTODOCK slightly favors the family of Fig 10C (pyridinium ring buried), with an energy of −7.03 kcal/mol. However, experimental evidence strongly suggests that the pyridinium ring is not buried: MKT-077 derivatives with larger poly-aromatics in place of the pyridinium moiety are equally or more potent73. In addition, through derivitization of the pyridinium moiety, MKT-077 could be covalently linked to Sepharose and be used to bind to HSPA9 75.
Prima facie, there seems little reason for the methylbenzothiazolin moiety (left in Fig 1) to seek the negatively charged pocket, since it does not carry a formal net charge. However, when using the AM1-BCC method76 to compute the charge distribution on MKT-077, one obtains a sizeable positive charge on this moiety due to polarization (see Fig 1). The charges obtained by the AM1-BCC method are known76 to correspond closely to the “true” quantum mechanical charges. In addition, one may expect MKT-077 to become even more polarized when placed in the vicinity of negative charges.
We became interested if one could distinguish between the different AUTODOCK poses by using the MMGB/PBSA solvation / desolvation computational protocol77 on basis of snapshots of molecular dynamics simulations of the complex (see Materials). We first tested whether we could distinguish between pyridinium “in” and “out” conformations. As detailed in the Materials section, carefully equilibrated 250 ps molecular dynamics runs for the complexes as shown in Figure 10, Panel C, (pyridinium “in”) and in Figure 10, Panel F, (pyridinium “out”) were obtained using Amber 11. We used the Generalized Born / Poisson Boltzmann protocol to estimate the solvation enthalpy of ligand, protein and complex (MMGB/PBSA). For the pyridinium “in” conformation (7.03 kcal/mol binding energy by AUTODOCK) a total AMBER binding enthalpy of −12.1 +/− 4.1 kcal/mol was obtained, while for the pyridinium “out” conformation (5.25 kcal/mol binding energy by AUTODOCK) an AMBER binding enthalpy of −18.3 +/− 2.9 kcal/mol was obtained (Table 2). This indicates a marked preference for the correct pyridinium “out” conformation in AMBER. Moreover, since the pyridinium is the only freely rotatable moiety in MKT-077 which is still relatively free to move in the pyridinium “out” conformation (from checking the trajectories), the pyridinium “out” conformation should also have a substantial entropic advantage as compared to the pyridinium “in” conformation. Having passed this test, we proceeded to try to distinguish between the different pyridinium “out” poses as shown in Figs 10 D, E and F, which have indistinguishable AUTODOCK energies of 6.32, 5.36 and 5.25 kcal/mol, respectively. As is shown in Table 2, the results of the Amber calculations are also too close to make this call, but the family shown in Fig 10F has the best score.
The AMBER results may be used to guide MKT-077 modifications that would allow tighter binding. No modification should be made on non-pyridinium moieties of the molecule since these parts are buried in the pocket. An obvious modification is to add positive charge to the compound, as it binds to an area that has overall strong negative electric potential as is shown in Figure 7. However, positively charged compounds are more toxic to the kidneys than neutral compounds78. Hence, adding extra charge to MKT-077 is likely to exacerbate the toxic side-effects79 which were cause for the termination of the Phase I trial54. MKT-077’s renal toxicity may potentially be reduced by converting MKT-077 to a zwitter-ion, with a negative functionality on the pyridinium moiety to complement the sidechain of His 227. His 227 is very close to the MKT pyridinium moiety in the best pose (see Tables Tables1,1, ,22 and Figure 11). By trying to exploit such an interaction, the compound’s affinity may be enhanced as well. However, the negative charge would likely interfere with MKT-077’s ability to selectively target the strongly negatively charged mitochondria of tumor cells80. Hence, the suggested conversion of MKT-077 to a zwitter-ion will likely take MKT-077 out of contention as an anti-tumor drug, but may allow it to be used in the treatment of tauopathies such as Alzheimer’s, potentially without the toxic renal side-effects found in the phase I trial. In fact, neutralization of the overall charge of MKT-077 could help enhance the compound’s ability to cross the blood-brain barrier81.
The pyridinium moiety is also close to an apolar surface area composed of residues V82 and T226. Modification of the pyridinium moiety to a larger poly-aromatic to exploit additional dispersion and hydrophobic interactions with these residues, may lead to a compound with a higher affinity. This may explain why the larger MKT-derivatives FJ-5744 and FJ-5826 are more potent HSPA inhibitors73.
We have determined that MKT-077 binds to a negatively charged pocket at the interface of the four HSPA8 NBD subdomains. All residues lining the pocket are conserved in all human HSPA isoforms. The compound binds selectively to the ADP state of the protein, and can hence be qualified as an allosteric drug. We suggest modifications that would potentially retain the compound’s binding affinity but which likely would diminish the compound’s renal toxicity.
The plasmid for the expression of bovine HSPA8 NBD domain (100% identical to the human protein) was a generous donation by Dr. D. B. McKay (Stanford School of Medicine, Stanford, CA). It was expressed in Escherichia coli strain BL21(DE3). In order to increase the yield of the induction, the cells were inoculated from a freshly transformed Petri dish a 2ml LB culture, were grown until OD(600)=0.4, transferred to a 100ml flask of M9 media and were grown until OD(600)=0.4. Subsequently the cells were centrifuged and transferred to a 1L M9 medium containing 98% D20, 2g/l protonated [13C glucose], and 1g/l 15NH4Cl. The expression was induced by isopropylthio-B-galactoside to 0.5mM at OD(600)=0.8. Harvested cells were removed by centrifugation and disrupted mechanically by a French press. Cell debris was removed by centrifugation and the supernatant was loaded onto a DEAE52 column and eluted with 150mM KCl. The Hsc70 fractions were dialyzed against 20mM HEPES (pH 7.0)/25mM KCl/10mM EDTA. The EDTA was precipitated by the addition of 25mM MgCl2 in the dialyzed protein pool yielding 5mM free Mg+2. The precipitate of (Mg)EDTA was removed by centrifugation. The supernatant was loaded onto an ATP-agarose affinity column and was eluted with 3mM ADP. The protein was concentrated by using Amicon concentrators. The protein was at all times stabilized with protein inhibitors and was kept at 4C.
For the assignment of the protein’s NMR spectrum, we used a 15N, 13C, 2H labeled 280 μM HSPA8-NBD sample that contained 5mM MgCl2, 25mM KCl, 20mM Tris-HCl, 10mM ADP, 5 mM K3PO4, 0.005% sodium azide and 10% (vol/vol) D20 (pH 7.2). The experiments were performed at 26 °C on a Varian Inova 800 MHz NMR spectrometer equipped with a triple-resonance cold probe. The protein backbone resonances were assigned from 3D HNCA, HN(CO)CA, HNCO, HN(CA)CO, HNCACB, HN(CO)CACB TROSY data. All spectra were processed with NMRPIPE82 and converted into Sparky83. The Sparky program was used to peak-pick the spectra. The peak pick lists were manually curated to obtain consistent NH root labeling throughout the different spectra. Assignments were made from these curated lists using the automatic assignment program SAGA60. Alternative assignments as produced by SAGA were evaluated and further checked using SPARKY. Only assignments of stretches of more then 2 connected spin systems were retained. By enlarge, they corresponded to the previous manual assignments for this protein domain 59.
The dye, originally manufactured by Fuji, Inc, is no longer commercially available. It was synthesized by us (Y.M. and J.E.G). The synthetic protocol will be described elsewhere. The 1H spectrum of MKT-077 in H2O was assigned using DQF-COSY and ROESY acquired on a 500 MHz Bruker Avance spectrometer. The ROESY spectra confirmed the trans configuration of the pyridinium ring and the nitrogen in the oxothiazolidin moiety over the double bond as shown in Fig 1.
HSPA8 NBD samples of 80 – 240 μM in 5mM MgCl2, 25mM KCl, 20mM Tris-HCl,10mM ADP, 5 mM K3PO4, 0.005% sodium azide and 10% (vol/vol) D20 (pH 7.2) were used for the titrations, using 4 mM solutions of MKT-077 in water (neutral pH) as titrant. The results of two titrations are reported here. Using a sample of 80 μM 15N-labeled HSPA8-NBD, MKT-077 was added to ratios of 1:1 and 2:1. Duplicate TROSY spectra of 8 hours each were recorded for each titration step. Using a triple-labeled 270 μM sample of HSPA8 NBD, MKT was titrated to ratios of 0.25:1, 0.5:1, 0.75:1 and 1:1. Duplicate TROSY spectra of 4 hours each were recorded for each titration step. The shifts upon the addition of the drugs were manually recorded in Sparky and were mapped on the crystal structure coordinates of HspA8 NBD (3HSC.pdb) using Pymol84.
The partial proteolysis protocol was adapted from a previously described method85. Briefly, HSPA8 (6 μM) in 40 mM HEPES buffer (20 mM NaCl, 8 mM MgCl2, 20 mM KCl, 0.3 mM EDTA, pH 8.0) was incubated with 1 mM nucleotide (ADP or ATP), either a buffer control or MKT-077 (200 μM) and a J-domain (residues 2-108, 24 μM) when noted. Samples were incubated at room temperature for 30 minutes. The trypsin (SIGMA Ec 184.108.40.206.4) was added to a final concentration of 0.9 μM and the samples were incubated for another 30 minutes at room temperature. The reaction was then quenched with the addition of 25 μl of 3x SDS loading buffer (240 mM Tris, 6 % w/v SDS, 30 % v/v glycerol, and 16 % v/v β-mercaptoethanol, 0.6 mg/ml bromophenol blue, pH 6.8) and heated to 95 °C for 3 minutes. Bands were separated by 12 % SDS-PAGE and stained with Coomassie blue.
AUTODOCK-486 was used for the docking of MKT-077 to HSPA8-NBD. The initial structure for MKT-077 was minimized in Jaguar (Schrödinger, LLC) at the B3LYP/6-31G* level. Standard charging methods within AUTODOCK leave MKT-077 uncharged. To obtain better charging models, we used the Antechamber87 suite in AMBER72. In one round of computations, we use Gasteiger charging as afforded by Antechamber, and just added +1 to the charge of the pyridinium nitrogen. We also used AM1-BCC charges as computed in AMBER. AM1-BCC predicts considerable polarization over the remainder of the conjugated molecule (see Fig 1) which also seems more realistic than the charge pattern in Gasteiger in which polarization is absent. The AMBER-charged models were hand-edited into the AUTODOCK. pdbqt files.
All available crystal structures for the NBD of HSPA8 conform to the closed (ATP) state61. These structures cannot be used for docking because MKT-077 does not bind to this state. In absence of a “true” high-resolution structure for the open ADP state, we chose one of the available crystal structures of HSPA8 in complex with a nucleotide exchange factor. According to the NMR analysis of a bacterial Hsp70 in the ADP and ATP state, HSPA8 in complex with a nucleotide exchange factor is a reasonable representation of the ADP state61. We chose HSPA8 in complex with yeast Hsp110 (3C7N.pdb88) as our model, because it is the only complex that contains ADP and Mg++. We discarded the coordinates of Hsp110 in the docking computations. In order to relieve possible strain exerted by crystal and/or Hsp110, the extracted HSPA8-NBD coordinates were relaxed using restrained minimization and equilibration in AMBER (see below). The coordinates of the equilibrated protein, including ADP and Mg++ were used for the AUTODOCK runs.
The AUTODOCK gridbox was located on the interface of the four subdomains IA-IB-IIA-IIB with a 0.2 Å resolution. We used a Lamarckian genetic algorithm with the following parameters : the GA runs were set to 100, the size of the initial population to 1500, the maximum number of evaluations was set to long, the maximum number of top individuals that automatically survive was set to 1, the rate of gene mutation was set to 0.02, the rate of crossover was 0.8, the GA crossover mode was twopt, the mean of Cauchy distribution for gene mutation was set to 0, the variance of Cauchy distribution for gene mutation was set to 1 and the number of generations for picking worst individuals was set to 10. The calculations were performed on a MacIntosh computer. The docked structures were clustered and evaluated by hand using Pymol84.
The crystal structure coordinates of HSPA8 in complex with yeast Hsp110, ADP, SO42− and Mg++ ions (3C7N.pdb) were used as a starting point. The coordinates of Hsp110 and SO42− ions were removed. The NBD, ADP and Mg coordinates were minimized in 1000 steps using a hybrid forcefield (ff99SB for the protein, gaff for metal ions and a gaff-derived forcefield89 for ADP) in the Amber 11 program suite72. The coordinates were restrained to the X-ray coordinates with a force constant of 5 kcal/mol/Å2. Subsequently we carried out a molecular Langevin dynamics equilibration run in implicit generalized Born solvent with 0.1 M ionic strength, running from 0 - 300 K over 10 ps. The same hybrid forcefield was used. The dynamics equilibration was restrained by 940 CA-CA distances in the 2-5 Å range, 2952 CA-CA distances in the 10-20 Å range, and 742 restraints between protein CA atoms and all atoms of ADP and Mg++ in the 1-15 Å range. The restraints had as lower bounds the actual distances as computed from the PDB file, and as upper bounds the actual distance + 1 Å, enforced by a parabolic potential of 20 kcal/mol/Å2. The calculations were performed on an Apple MacBookPro5.1 computer equipped with a 64-bit 2.4 GHz Inter Core 2 Duo processor, running MacOS X10.6.6.
The AUTODOCK solutions with “energies” of 7.03, 6.32, 5.36 and 5.25 kcal /mol were evaluated using AMBER (vs. 11) in the following way. Hydrogen-atoms were re-attached to the coordinates of the docked MKT-077 molecules and the double and aromatic bonds were reassigned in the Pymol Builder module84. These coordinates were converted to AMBER .prepi and forcefield modification files using the Amber Antechamber suite. We used AM1-BCC charging for MKT-077 as shown in Fig 1.
In order to carry out a proper MD-based binding energy evaluation, it is necessary to run the simulations using explicit solvation. For a molecule the size of HSPA8 NBD (380 residues) such computations become too time consuming for our labs, which do not have access to specialized super computers. Hence, we decided to carry out restrained MD simulations on a docking site consisting of HSPA8 residues 12-13+69-83+143-158+172-177+196-211+219-229+316-323 and the Mg++ ion, which was extracted from the equilibrated pdb files (see Figure S3). Four different docked MKT-077 poses, corresponding to the best members of each of the four families shown in Fig 10, were added the site (not simultaneously). Identical protocols were followed for the four computational series. The “complexes” were solvated with ~6500 TIP3P waters in a 12 Å3 periodic box. After a minimization step, a molecular dynamics temperature ramp running from 0 - 300 K over 50 ps was carried out. The same hybrid force field was used as described above. This run was restrained by 3790 restraints between N, HN, CA, C’ and O backbone atoms of the reduced binding site, 55 Mg++ - CA restraints, and ~2600 MKT - CA restraints based on the minimized structure (depending on family member). All restraints were defined within a 1.0 Å range and enforced with a 20 kcal/mol/Å2 gradient. Subsequently, a density equilibration (for 50 ps, with pressure relaxation time of 1 ps) and general equilization (for 100 ps, with pressure relaxation time of 2 ps) with the same restraints followed. These equilibration steps were followed by a production run of 250 ps restrained MD computations at 300 K. In the production runs, the MKT restraints were removed, while all others remained. The binding enthalpies were calculated using the MM-GB/PBSA protocol77 as implemented in the Amber 11 release, using 100 frames of the last 50 ps of the molecular dynamics production runs. The calculations were performed on an Apple MacBookPro5.1 computer equipped with a 64-bit 2.4 GHz Inter Core 2 Duo processor, running MacOS X10.6.6.
We thank Dr. Dan Weaver for the sample of triple-labeled HSPA8-NBD. We thank Mr. Peter Ung (lab of Dr. Heather Carlson) for tutorials on AMBER and AUTODOCK and Dr. Heather Carlson for the quantum mechanical calculations on isolated MKT-077. Mr Ung also provided parameters for ADP in AUTODOCK. We thank Dr. Daniel Roe (lab of Dr. David Case) for help with the AM1-BCC charging method in AMBER. Support from NIH grants GM63027-S02 (ERPZ), NS059690 (AR, ERPZ and JEG) and NS073899 and AG031291 (CAD) is gratefully acknowledged. CAD also thanks the Alzheimer’s Association for support.
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